| 出品公司: | Addgene |
|---|---|
| 载体名称: | PX390 或 PX390 Flavobacterium columnare Cas9 |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 11801 bp |
| 5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
| 载体标签: | NLS (C ter)；HA、NLS (N ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 昆虫细胞 |
| 备注: | -- |
| 产品目录号: | #68327 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
PX390载体质粒基本信息
PX390质粒图谱载体图谱和PX390载体序列质粒序列多克隆位点信息
PX390质粒载体简介
PX390质粒序列载体序列
LOCUS PX390 Flavobacterium columnare Cas9 11801 bp DNA SYN
DEFINITION PX390 Flavobacterium columnare Cas9
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11801
/organism="PX390 Flavobacterium columnare Cas9"
/mol_type="other DNA"
misc_feature 47..1001
/label="Ac_5_flank"
CDS complement(343..948)
/label="ORF frame 3"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1023..1587
/label="CMV_immearly_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1090..1377
/label="CAG_enhancer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1544..1564
/label="CMV_fwd_primer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1615..1633
/label="T7_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1633..1656
/label="lacO"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1772..1796
/label="attB1"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
CDS 1822..6492
/label="ORF frame 1"
/translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
FQKI*"
misc_feature 1825..1851
/label="HA"
/translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
FQKI*"
misc_feature 1855..1875
/label="SV40 NLS"
/translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
FQKI*"
misc_feature 1861..1875
/label="NLS"
/translation="MYPYDVPDYASPKKKRKVEASMTKILGLDLGTNSIGWAIRDTEN
EGINQILDKGARIFSEGVKSENGKEISRAAERTAYRSARKIKYRRKLRKYETLKVLSI
NGMCPLSVEEVEQWKKSGFKEYPLNPEFLDWLRTNEDKNINPYLFRDKASKQKVTLFE
LGRALYHIAQRRGFLSNRLDQSAEGVFEEHNPQIQNLIEDLDSSNTILNELKEYYINL
GIIDETEKSGFKKDLDEGEKKLKSLYNSLVAITKKNANDIETCKQELIARLNKKEDLG
KVKGKIKDISQAMLDRNFKTLGQYFFSLYNKERIRNQYTSREEHYLEEFIIICQTQGI
EGINTNEKLPEKKFTGLAKDLYRAIFFQRPLKSQKGLIGKCSFEKNKSRCAVSHPDYE
EFRMWSYLNTIKIGTQSEKTLRFLTLEEKLKLVPKFYRKSDFNFEVLAKELVEKGASF
GYYKSSKKNEFFYWFNYKPTDSVSACVVSASLENAIGKDWKIKTFEYQTRNTEKNEVT
KSVDYKDLWHLLSVATSDTYLYDFAIEKLKLEPKNAKAFSKTKLKKDFASLSLAAIAK
ILPYLKQGLLYSHAVFMANIENIVDADIWKDEEQQKFIQSKIVELVDNYIVEKSKLEL
INGLLKIYNTEDKEGRKVYYSKEAESLFEADLRKKLVPFYKANIIIEEHEQEIIFQDL
FPILMDQLKKQEFIKIGRLDKQIEAFLEGENEEGQIFCNHTDKLKKLYHPSDIEVFKK
KTIKDEWGNEKVVLGSPLTTSIKNPMAMRALHQLRKVLNTLIANDQIDEDTRIHIEMA
RELNDANKRKGIQDFQNENKKFREEAIKEIKKLYLEECHKDVNPTEDDILRYQLWLEQ
GKCEIYEEGNNISICDIIGSNPSYDIEHTIPRSISQDNSQMNKTLCSLKFNRSIKKQK
MPVELSNYNDILPRIAHWKKEAEELTRQIETISRSIKSAATKVAKDKNIRKRHYLTLK
RDYILGKYERFTWEEPKVGFKNSQIPDTGIITKYAQAYLKSYFKRVESVKGGAVAEFR
KLWGIQESFIDENWWKHYKDKDRDKHTHHTIDAITIACMPKDKYDLLAHAWRLEDEQD
KKAAKVLIEQAKPWKTFKEDIEKIETEILVSHFTPDNVKKQSKSIIKNRGKKVYVLKN
ELPVNFKNKIEGKDYFKLKFDSKILYKIPKKKEKQTDTFYEELPKNYLNGVEGKDYFK
INTTGKTFYKIPIFNQGDTIRGSLHQETTYGAIKLPDIDIETKKPLHTDKGGFILKKD
IKGNEIVFFVVRKEISKISENDVQNIVDNVVRKKIENAIANSLITFKIVKKKKVAVIK
EGVTIWMREPNIEKGIEGIPIKKVRIITNSVKNPIEIKVHSPLSKSRHKHKQKVYGQN
DENYAMALYELDGKREFELINNFNLAKLLKQSQSYYPLHKEKEIKGKKILVPIEKRNN
KDVILKRGQQVVFYDKTVENPKDISEIIDFRERIYIIEGLTIQRQKDKKTSKVNEYGI
IQLRHFKEARKSEEISKDNFKPDGEFKINENKPTRKMNHNQFTAFVEGIDFKVLPSGK
FQKI*"
CDS complement(3754..5181)
/label="ORF frame 3"
/translation="MAHQDFSLNLFDILLEGFPWFGLLDQHLSGLFVLFILQPPGVCQ
QVVFILGHTGNSDCVDCMVGMFVSIFVLIVLPPVLIDKRFLDAPQFPELSHCSSLDTL
HPFEVRFQICLSVLGNDPSVWDLGVFKAHFRFLPSEPLVFPQNIVSLQCQVMPLSDVL
ILGHLGCSALDAARNGFDLPSQFLGLLFPVCDPGQNVIVVGQLHWHLLLFDGSVELQA
TQCLVHLAVILGDAPRNGMLDVIGGIAANDITNADIVAFLVDLALSLFQPQLIPQDIV
LGGVHVFVAFLQVQLLDLLDGFLPKLLVLVLEVLDAFSLISIVQFSGHLNMDPSILVD
LVVGNQCIQHLPQLVQGSHCHGVLDRCGQWGSQDHLFIPPLIFDSLFLKHLDVAWMVQ
FLQLICVVTKDLSFLIFPLQEGFNLLIQSANFDELLFLQLVHQDRKQILENDFLLVFL
DDDVSLVEGDQFLSQVCLEQAFSLL*"
CDS complement(3810..4295)
/label="ORF frame 1"
/translation="MASSRNFLFSFWKSWMPFRLLASFSSLAISIWIRVSSSIWSLAI
SVFSTFLSWCRALIAMGFLIDVVSGDPRTTFSFPHSSLIVFFLNTSMSLGWYSFFSLS
VWLQKICPSSFSPSRKASICLSSLPILMNSCFFSWSIRIGNRSWKMISCSCSSMMMLA
L*"
CDS complement(5209..5931)
/label="ORF frame 3"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
misc_feature complement(6525..6542)
/label="Sp6_primer"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
misc_feature 6547..6571
/label="attB2"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
terminator 6583..7049
/label="hGH_PA_terminator"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
terminator 7055..7090
/label="T7_terminator"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
rep_origin 7207..7513
/label="f1_origin"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
misc_feature complement(7627..7647)
/label="pBABE_3_primer"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
misc_feature complement(7633..7848)
/label="SV40_enhancer"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
promoter 7645..7913
/label="SV40_promoter"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
rep_origin 7812..7889
/label="SV40_origin"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
misc_feature 7874..7893
/label="SV40pro_F_primer"
/translation="MLVARLGQWTVHLNLDRVFDRIGNDAHFLNRDSLDPLFNVGLPH
PNGDPLFDDGHFLFLHDLKSDQGVCDSILNFLPDHVVDDILHIVLTNFRDFLADHKEH
NLIAFDVFLQNEASLISMQGFLCFNVDIWQLNGSVSCLLVQAPPDCVALVKNWDFVEG
LAGSIDFEVVLAFHSIQVVFRQFLIECIGLLLFLFGDFVQNLAVKFQLEIILPFDLVL
EVHRQLVLQDVHLFAAILDDAL*"
CDS 8028..8822
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 8031..8819
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(8337..8873)
/label="ORF frame 1"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 8999..9118
/label="SV40_PA_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 9087..9106
/label="EBV_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 9309..9344
/label="T7_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature complement(9367..9384)
/label="Baculovirs_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 9374..9451
/label="3_AcPH"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS complement(9481..10098)
/label="ORF frame 3"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
misc_feature 10078..10111
/label="loxP"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
gene complement(10879..11739)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
CDS complement(10879..11739)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatgacc aaaatcctgg ggctggacct ggggactaat
1921 agcatcgggt gggcaatccg cgacacagaa aatgagggca tcaatcagat cctggacaag
1981 ggcgcccgca ttttcagcga gggagtgaag tccgagaatg gcaaagaaat cagtagggca
2041 gctgaacgaa ccgcttaccg gagcgcaaga aagatcaaat atcggagaaa actgcggaag
2101 tacgagacac tgaaggtgct gtcaattaac ggaatgtgcc ccctgagcgt ggaggaagtc
2161 gaacagtgga agaaatctgg cttcaaggaa tatcccctga atcctgagtt tctggattgg
2221 ctgcggacca acgaggacaa gaacatcaat ccttacctgt tcagagataa agctagcaag
2281 cagaaagtga cactgtttga actgggaaga gctctgtatc acatcgcaca gaggcgcggc
2341 ttcctgagta acaggctgga ccagtcagcc gagggcgtct ttgaggaaca taatcctcag
2401 atccagaacc tgattgagga cctggacagc tccaacacaa ttctgaatga gctgaaggaa
2461 tactatatca atctggggat cattgacgag actgaaaagt ccggcttcaa gaaagacctg
2521 gatgaggggg aaaagaaact gaagtcactg tacaacagcc tggtggccat cacaaagaaa
2581 aacgctaacg atatcgaaac ttgcaagcag gagctgatcg ctagactgaa taagaaagag
2641 gacctgggca aggtcaaagg gaagatcaaa gatatttctc aggcaatgct ggacaggaac
2701 tttaagactc tgggacagta tttcttttca ctgtacaaca aggagcgaat ccggaaccag
2761 tataccagcc gcgaggaaca ctacctggag gagttcatca ttatctgtca gacccagggg
2821 atcgagggaa ttaacacaaa tgagaagctg cctgagaaga agttcaccgg cctggcaaaa
2881 gatctgtatc gagccatttt ctttcagcgg ccactgaaat cccagaaggg cctgatcggg
2941 aagtgctctt tcgagaagaa caagtctcgc tgtgccgtga gtcacccaga ctatgaggaa
3001 tttcgaatgt ggagctacct gaacacaatc aaaattggca ctcagtccga gaaaaccctg
3061 cgcttcctga cactggagga aaagctgaaa ctggtgccca agttctaccg aaagagtgat
3121 ttcaacttcg aggtgctggc taaggagctg gtcgaaaaag gagcatcatt cggctactac
3181 aagtctagta agaaaaatga gttcttttac tggttcaact ataagcccac cgattcagtg
3241 agcgcctgcg tggtgagcgc ttctctggag aacgcaatcg gcaaggactg gaagatcaag
3301 actttcgaat atcagactag aaacaccgag aagaatgaag tgaccaaatc cgtcgattac
3361 aaggacctgt ggcacctgct gtccgtggca acatctgata cttacctgta tgactttgcc
3421 atcgaaaagc tgaaactgga gcctaaaaac gcaaaggcct tcagcaagac aaaactgaag
3481 aaagactttg ccagtctgtc actggcagcc atcgctaaaa ttctgccata tctgaagcag
3541 ggcctgctgt actcccacgc cgtgttcatg gctaacatcg agaatattgt cgacgccgat
3601 atctggaagg atgaggaaca gcagaagttc atccagtcca agattgtgga gctggtcgac
3661 aattatatcg tggaaaagtc taaactggag ctgatcaatg ggctgctgaa gatctacaac
3721 accgaggata aggaaggacg gaaagtgtac tattcaaagg aggctgaaag cctgttcgag
3781 gcagacctga gaaagaaact ggtccccttc tacaaggcta acatcatcat cgaggaacac
3841 gagcaggaaa tcattttcca ggatctgttt cctatcctga tggaccagct gaagaaacag
3901 gagttcatca aaattggcag actggataag cagattgaag ccttcctgga gggggaaaat
3961 gaggaaggac agatcttttg taaccacaca gataagctga agaaactgta ccatccaagc
4021 gacatcgagg tgtttaagaa aaagactatc aaagatgagt gggggaatga aaaggtggtc
4081 ctgggatccc cactgaccac atctatcaag aaccccatgg caatgagagc cctgcaccag
4141 ctgaggaagg tgctgaatac actgattgcc aacgaccaga tcgacgagga tactcggatc
4201 catattgaga tggccagaga actgaacgat gctaataagc gaaaaggcat ccaggacttc
4261 cagaacgaga acaagaagtt tcgggaggaa gccatcaagg agatcaagaa gctgtacctg
4321 gaggaatgcc acaaagacgt gaaccccacc gaggacgata tcctgaggta tcagctgtgg
4381 ctggaacagg gaaagtgcga gatctacgag gaaggcaaca atatcagcat ttgtgatatc
4441 attggcagca atccctccta tgacatcgag cataccattc ctcggagcat ctcccaggat
4501 aacagccaga tgaacaagac actgtgtagc ctgaagttca acagatccat caaaaagcag
4561 aagatgccag tggagctgtc caactacaat gacattctgc ccaggatcgc acactggaaa
4621 aaggaggccg aggaactgac taggcagatc gaaaccattt ctcgcagcat caagagcgct
4681 gcaaccaagg tggccaagga taagaacatc agaaagaggc attacctgac actgaagcga
4741 gactatattc tggggaaata cgagcggttc acttgggagg aacctaaagt gggctttaaa
4801 aactcccaga tcccagacac tgggatcatt accaagtacg ctcaggcata tctgaaatct
4861 tacttcaaaa gggtggagag tgtcaaggga ggagcagtgg ctgagttccg gaaactgtgg
4921 ggcatccagg aatcttttat cgatgagaac tggtggaagc actataagga caaagataga
4981 gacaaacata cccaccatac aatcgacgca atcactattg cctgtatgcc caaggataaa
5041 tacgacctgc tggcacacgc ctggaggctg gaggatgaac aggacaaaaa ggccgctaag
5101 gtgctgatcg agcaggccaa accatggaaa accttcaagg aggatatcga aaagattgag
5161 actgaaatcc tggtgagcca ttttaccccc gacaacgtca aaaagcagtc aaagagcatc
5221 atcaagaatc gcggcaaaaa ggtgtacgtc ctgaagaacg agctgcctgt gaacttcaag
5281 aacaagatcg aagggaagga ttatttcaag ctgaaatttg acagcaagat tctgtacaaa
5341 atccccaaaa agaaagagaa gcagaccgat acattctatg aggaactgcc taaaaactac
5401 ctgaatggag tggaaggcaa ggactacttc aaaatcaata ctaccggcaa gaccttctac
5461 aaaatcccaa tttttaacca gggcgacaca atccggggga gcctgcacca ggagacaact
5521 tacggagcca ttaagctgcc agatatcgac attgaaacaa agaaacccct gcatactgat
5581 aagggaggct tcattctgaa gaaagacatc aaaggcaatg agattgtgtt ctttgtggtc
5641 cgcaaggaaa tctctaaaat tagtgagaac gatgtgcaga atatcgtcga caacgtggtc
5701 cggaagaaaa ttgagaatgc tatcgcaaac tccctgatca cttttaagat cgtgaagaaa
5761 aagaaagtgg ccgtcatcaa agagggggtc accatttgga tgagggagcc caacattgaa
5821 aaggggatcg agggaatccc tattaagaaa gtgcgcatca ttaccaattc tgtcaaaaac
5881 cctatcgaga ttaaggtgca cagtccactg tccaagtctc gccacaagca taaacagaag
5941 gtctacggcc agaacgatga gaattatgcc atggctctgt acgaactgga cgggaagcgg
6001 gagttcgaac tgatcaacaa ttttaatctg gccaagctgc tgaaacagag tcagtcatac
6061 tatccactgc ataaagagaa ggaaatcaag ggaaagaaaa ttctggtgcc catcgagaag
6121 agaaacaaca aggatgtcat cctgaagagg ggccagcagg tggtgttcta cgacaaaacc
6181 gtggagaacc ccaaggatat ctctgaaatc attgactttc gcgagcgaat ctacatcatt
6241 gaagggctga ccatccagag acagaaagat aagaaaacat ccaaagtgaa tgagtacgga
6301 atcattcagc tgcgccactt caaggaagct cgaaaaagtg aggaaatctc aaaggataac
6361 ttcaaacctg acggcgagtt caagatcaac gagaataagc caactaggaa aatgaaccat
6421 aatcagttca ccgcctttgt ggaggggatc gacttcaagg tcctgcctag cggaaagttt
6481 cagaaaattt aagaattcgg taccgagctc aagcttgcgg ccgcctatag tgtcacctaa
6541 atcgtaaccc agctttcttg tacaaagtgg tcgggtggca tccctgtgac ccctccccag
6601 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
6661 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
6721 tggtatggag caaggggcaa gttgggatga caacctgtag ggcctgcggg gtctattggg
6781 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
6841 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
6901 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
6961 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
7021 cgtgaaccac tgctcccttc cctgtccttc tagcataacc ccttggggcc tctaaacggg
7081 tcttgagggg ttttttgtct agaagacaat agcaggcatg ctggggatgc ggtgggctct
7141 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
7201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
7261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
7321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
7381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
7441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
7501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
7561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
7621 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
7681 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
7741 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
7801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
7861 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
7921 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
7981 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
8041 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
8101 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
8161 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
8221 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
8281 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
8341 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
8401 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
8461 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
8521 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
8581 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
8641 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
8701 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
8761 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
8821 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
8881 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
8941 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
9001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
9061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
9121 tgtctgttca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
9181 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
9241 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
9301 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagca
9361 tgcggaggaa attctccttg aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga
9421 cgcacctctg ttcactggtc cggcgtatta aaacacgata cattgttatt agtacattta
9481 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
9541 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
9601 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
9661 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
9721 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
9781 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
9841 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata gagcttggac
9901 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
9961 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
10021 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaattg cgatcgcata
10081 acttcgtata gcatacatta tacgaagtta taagctcgga acgctgcgct cggtcgttcg
10141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
10201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
10261 ggccgcgttg ctggcgtttt tccataggct ccgccccctg acgagcatca caaaaatcga
10321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
10381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
10441 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
10501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
10561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
10621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
10681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
10741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
10801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
10861 tctcaagaag atcctttgtt accaatgctt aatcagtgag gcacctatct cagcgatctg
10921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
10981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
11041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
11101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
11161 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
11221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
11281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
11341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
11401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
11461 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
11521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
11581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
11641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
11701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
11761 ttgaagcatt tatcagggtt attgtctcat gtccgcgcgt t
//
