| 出品公司: | Clontech |
|---|---|
| 载体名称: | pGRE-luc |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5.7kb |
| 5' 测序引物及序列: | RVprimer3: CTA GCA AAA TAG GCT GTC CC |
| 3' 测序引物及序列: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳定 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGRE-luc载体质粒基本信息
pGRE-luc质粒图谱载体图谱和pGRE-luc载体序列质粒序列多克隆位点信息
pGRE-luc质粒载体简介
pGRE-luc(报告基因质粒)是用于检测糖皮质激素响应元件(glucocorticoid response element, GRE)转录激 活水平的报告基因质粒。pGRE-luc是以pGL6质粒为模板，在其多克隆位点插入了多个GRE位点，可以高灵敏度 地检测GRE的激活水平，检测糖皮质激素介导的信号途径(glucocorticoid-mediated signaling pathway)的激活水平。
pGL6质粒是用于在哺乳动物细胞中进行萤火虫荧光素酶(firefly luciferase)报告基因检测的新一代质粒。该报告基因质粒比 Promega公司的pGL3系列有了全面的改进，一方面对于luciferase的编码进行了改进，确保能更好地在哺乳动物细胞中进行 表达，同时对整个质粒中所有可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理，在保持原有功能不 变的情况下，使各种转录因子在质粒上的非特异性结合降到最低。
萤光素、萤光素酶、萤火虫萤光素酶和海肾萤光素酶也经常被称作荧光素、荧光素酶、萤火虫荧光素酶和海肾荧光素酶。
pGRE-luc质粒序列载体序列
Snapgene格式序列下载: pGRE-Luc Sequence
LOCUS pGRE-Luc 5615 bp DNA circular SYN 10-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5615)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 10, 2025 from SnapGene 8.1.0
https://www.snapgene.com
COMMENT Sequence Label: pGRE-Luc
FEATURES Location/Qualifiers
source 1..5615
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 110..119
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 116..1768
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1812..1933
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2127..2484
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2127..2318
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
rep_origin 2335..2470
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2515..3309
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3334..3382
/note="synthetic polyadenylation signal"
rep_origin complement(3709..4297)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(join(4497..5288,5289..5357))
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 5462..5510
/note="synthetic polyadenylation signal"
misc_feature 5524..5615
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtaccg ctagcggtac attttgttct agaacaaaat gtaccggtac
61 attttgttct agatctgcag aagcttggca atccggtact gttggtaaag ccaccatgga
121 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
181 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
241 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
301 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
361 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
421 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
481 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
541 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
601 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
661 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
721 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
781 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
841 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
901 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
961 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
1021 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
1081 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
1141 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
1201 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
1261 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
1321 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
1381 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
1441 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
1501 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
1561 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
1621 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
1681 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
1741 caagaagggc ggcaagatcg ccgtgtaata attctagagt cggggcggcc ggccgcttcg
1801 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
1861 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
1921 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
1981 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
2041 gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga aataacctct
2101 gaaagaggaa cttggttagc taccttctga ggcggaaaga accagctgtg gaatgtgtgt
2161 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
2221 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
2281 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
2341 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
2401 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
2461 tttggaggcc taggcttttg caaaaagctc gattcttctg acactagcgc caccatgatc
2521 gaacaagacg gcctccatgc tggcagtccc gcagcttggg tcgaacgctt gttcgggtac
2581 gactgggccc agcagaccat cggatgtagc gatgcggccg tgttccgtct aagcgctcaa
2641 ggccggcccg tgctgttcgt gaagaccgac ctgagcggcg ccctgaacga gcttcaagac
2701 gaggctgccc gcctgagctg gctggccacc accggcgtac cctgcgccgc tgtgttggat
2761 gttgtgaccg aagccggccg ggactggctg ctgctgggcg aggtccctgg ccaggatctg
2821 ctgagcagcc accttgcccc cgctgagaag gtttctatca tggccgatgc aatgcggcgc
2881 ctgcacaccc tggaccccgc tacctgcccc ttcgaccacc aggctaagca tcggatcgag
2941 cgtgctcgga cccgcatgga ggccggcctg gtggaccagg acgacctgga cgaggagcat
3001 cagggcctgg cccccgctga actgttcgcc cgactgaaag cccgcatgcc ggacggtgag
3061 gacctggttg tcacacacgg agatgcctgc ctccctaaca tcatggtcga gaatggccgc
3121 ttctccggct tcatcgactg cggtcgccta ggagttgccg accgctacca ggacatcgcc
3181 ctggccaccc gcgacatcgc tgaggagctt ggcggcgagt gggccgaccg cttcttagtc
3241 ttgtacggca tcgcagctcc cgacagccag cgcatcgcct tctaccgctt gctcgacgag
3301 ttcttttaat gatctagaac cggtcatggc cgcaataaaa tatctttatt ttcattacat
3361 ctgtgtgttg gttttttgtg tgttcgaact agatgctgtc gaccgatgcc cttgagagcc
3421 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
3481 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc
3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
4141 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
4441 tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac
4501 cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg
4561 gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc
4621 gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag
4681 ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct
4741 atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt
4801 gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac
4861 tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc
4921 agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg
4981 gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg
5041 accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct
5101 tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc
5161 atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt
5221 tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt
5281 tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga
5341 aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac
5401 tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg
5461 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
5521 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
5581 ccccagtgca agtgcaggtg ccagaacatt tctct
//
pGRE-luc载体图谱质粒图谱pdf版和pGRE-luc载体序列质粒序列等相关资料下载
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