| 质粒名称: | pME-Cas9-T2A-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 63155 |
| 存储实验室: | Leonard Zon |
|
载体骨架基本信息 |
|
| 载体名称: | pME-MCS (Tol2Kit #237) |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 2765 bp |
| 完整质粒大小: | 7685 bp |
| 载体类型: | CRISPR；Tol2网关中间入口向量 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9-T2A-GFP |
| 物种来源: | D. rerio (zebrafish) |
| 插入大小: | 4984 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SalI(未破坏) |
| 3' 克隆位点: | NotI(未破坏) |
| 5' 测序引物 | CCTACTCAGGAGAGCGTTCA |
| 3' 测序引物 | CAGGAAACAGCTATGACCAT |
|
文献引用方法 |
|
| 材料和方法部分: | pME-Cas9-T2A-GFP was a gift from Leonard Zon (Addgene plasmid # 63155 ; http://n2t.net/addgene:63155 ; RRID:Addgene_63155) |
| 参考文献部分: | A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish. Ablain J, Durand EM, Yang S, Zhou Y, Zon LI. Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032. 10.1016/j.devcel.2015.01.032 PubMed 25752963 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pME-Cas9-T2A-GFP 7685 bp ds-DNA circular SYN 29-AUG-2018
DEFINITION Middle Entry clone for Gateway containing a zebrafish
codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP.
Cas9 and GFP are separated by the sequence of a T2A self-cleaving
peptide..
ACCESSION .
VERSION .
KEYWORDS pME-Cas9-T2A-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7685)
AUTHORS Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
TITLE A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in
Zebrafish.
JOURNAL Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi:
10.1016/j.devcel.2015.01.032.
PUBMED 25752963
REFERENCE 2 (bases 1 to 7685)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 29, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7685
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 99..116
/label=L4440
/note="L4440 vector, forward primer"
terminator 275..302
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 394..480
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 529..551
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 544..560
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 576..675
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind 677..693
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 703..721
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 757..766
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 775..795
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 802..4902
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes MGAS15252 Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 4815..4823
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 4918..4938
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4954..5007
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5014..5733
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5059..5080)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5320..5339)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5667..5688
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(5770..5790)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(5770..5788)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
protein_bind complement(5817..5916)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(5934..5952)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5957..5973)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5957..5973)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6075..6094)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 6086..6895
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(6153..6172)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 7042..7630
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7531..7550
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
61 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
121 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
181 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
241 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
301 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
361 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
421 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
481 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
541 gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga
601 tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt
661 acaaaaaagc aggcttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
721 ggcgaattgg gtaccgggcc ccccctcgag gtcgacgcca ccatggcttc tccacctaag
781 aagaagagaa aggtgggaag catggataag aagtatagca tcggcctgga tattggaact
841 aactccgtgg gttgggcagt gattacagac gactacaagg tccctagcaa gaaatttaag
901 gtgctgggta acaccgacag gcacagcatc aagaaaaatc tgattggagc cctgctgttc
961 ggttctggag agactgccga agcaacacgc ctgaaaagaa cagcaagaag gcgctatacc
1021 agaaggaaga atagaatctg ttacctgcag gagattttct ctaacgaaat ggctaaggtg
1081 gacgattcat tctttcatag gctggaggaa agtttcctgg tcgaggaaga taagaaacac
1141 gagcgccatc ctatctttgg aaacattgtg gacgaggtcg cctatcacga aaaataccca
1201 accatctatc atctgcgcaa gaaactggct gactctactg ataaagccga cctgagactg
1261 atctatctgg ctctggccca catgattaag ttcaggggtc attttctgat cgagggcgat
1321 ctgaaccccg acaattccga tgtggacaag ctgttcatcc agctggtcca gatttacaat
1381 cagctgtttg aggaaaaccc tattaatgct tccagagtgg acgcaaaagc tatcctgtca
1441 gccaggctgt ccaagtcacg cagactggag aacctgattg cacagctgcc cggagaaaag
1501 aggaacggtc tgtttggaaa tctgatcgct ctgagtctgg gcctgactcc taacttcaaa
1561 agcaattttg atctggctga ggacgccaaa ctgcagctgt caaaggacac atatgacgat
1621 gacctggata acctgctggc acagatcgga gatcagtacg ctgacctgtt cctggctgcc
1681 aaaaatctgt ccgacgcaat cctgctgtca gatattctga gagtgaacag cgagattaca
1741 aaagcacctc tgagtgccag catgatcaag agatatgacg agcaccatca ggatctgacc
1801 ctgctgaagg ctctggtcag gcagcagctg ccagagaagt acaaggaaat tttctttgat
1861 cagtccaaga acggctacgc cggttatatc gacggaggcg catcacagga ggaattctac
1921 aagtttatca aacctattct ggagaagatg gacggaactg aggaactgct ggtgaaactg
1981 aatagagagg acctgctgag gaagcagcgc acatttgata acggttccat cccacaccag
2041 attcatctgg gagagctgca cgctatcctg aggcgccagg aagacttcta cccctttctg
2101 aaagataacc gcgagaagat cgaaaaaatt ctgaccttca gaatccctta ctatgtgggt
2161 ccactggctc gcggaaacag cagatttgcc tggatgactc gcaaatccga ggaaaccatt
2221 actccttgga acttcgagga agtggtcgat aagggcgcct ctgcacagtc cttcatcgag
2281 agaatgacta attttgacaa aaacctgccc aatgagaaag tgctgcctaa gcactccctg
2341 ctgtacgagt atttcactgt ctataacgaa ctgacaaagg tgaaatacgt caccgagggc
2401 atgagaaagc cagccttcct gtcaggagag cagaagaaag caatcgtgga tctgctgttt
2461 aaaaccaaca ggaaagtgac tgtcaagcag ctgaaggagg actacttcaa gaaaattgaa
2521 tgcttcgatt ccgtggagat cagcggagtc gaagacagat ttaacgcaag cctgggcgct
2581 taccacgatc tgctgaagat cattaaggat aaagacttcc tggacaacga ggaaaatgag
2641 gatatcctgg aagacattgt gctgacactg accctgtttg aggacagagg aatgatcgag
2701 gaaagactga aaacctatgc tcatctgttc gatgacaagg tgatgaaaca gctgaagaga
2761 aggcgctaca ctggctgggg tagactgagc aggaagctga tcaacggcat tagggataaa
2821 cagtcaggaa agacaatcct ggactttctg aaaagtgatg gcttcgccaa ccgcaatttt
2881 atgcagctga ttcacgatga cagtctgacc ttcaaagagg acatccagaa ggctcaggtg
2941 tctggacagg gccactccct gcatgagcag attgcaaacc tggctggaag cccagccatc
3001 aagaaaggca ttctgcagac agtgaaaatc gtcgatgagc tggtgaaagt catgggccat
3061 aagcccgaaa acatcgtgat tgagatggct cgcgaaaatc agacaaccca gaagggtcag
3121 aagaacagta gagagaggat gaaaagaatc gaggaaggca ttaaggagct gggtagccag
3181 atcctgaaag agcacccagt ggaaaacaca cagctgcaga atgagaagct gtatctgtac
3241 tatctgcaga atggaagaga tatgtacgtg gaccaggagc tggatattaa caggctgtct
3301 gattacgacg tggatcatat cgtcccccag agtttcatca aagatgacag cattgacaac
3361 aaggtgctga ccaggtccga caaaaacaga ggaaaatcag ataatgtccc tagtgaggaa
3421 gtggtcaaga aaatgaagaa ctactggaga cagctgctga atgccaaact gatcactcag
3481 aggaagtttg ataacctgac aaaagcagag cgcggtggac tgtcagaact ggacaaagct
3541 ggattcatca agaggcagct ggtggaaaca cgccagatca ctaaacacgt cgcacagatt
3601 ctggatagtc gcatgaacac aaagtacgat gagaatgaca aactgatcag agaagtgaag
3661 gtcattaccc tgaagagtaa actggtcagc gactttagga aagatttcca gttttataag
3721 gtccgcgaga ttaacaatta tcaccatgcc catgacgcat acctgaacgc cgtggtcggt
3781 accgcactga tcaagaaata cccaaaactg gagagcgaat tcgtgtacgg agactataag
3841 gtgtacgatg tcagaaaaat gatcgccaag tccgagcagg aaattggaaa agctactgcc
3901 aagtatttct tttactcaaa catcatgaat ttctttaaga cagagatcac cctggccaat
3961 ggagaaatcc gcaaaaggcc cctgattgag acaaacggag agacaggcga aatcgtgtgg
4021 gacaaaggca gagattttgc aaccgtgagg aaggtcctga gcatgcctca agtgaatatc
4081 gtcaagaaaa ctgaggtgca gacaggcggt ttctcaaaag aaagtattct gccaaaacgc
4141 aactctgata agctgatcgc tagaaagaaa gactgggacc ctaagaagta tggaggcttt
4201 gactctccca ctgtggcata ctccgtcctg gtggtcgcta aggtggagaa gggcaaaagc
4261 aagaaactga aatctgtcaa ggagctgctg ggtatcacaa ttatggagag aagctctttc
4321 gagaagaacc caatcgattt tctggaggcc aaaggttata aggaagtgaa gaaagacctg
4381 atcattaaac tgcccaagta cagtctgttt gagctggaaa acggcaggaa acgcatgctg
4441 gcaagcgctg gagagctgca gaaaggcaat gaactggccc tgccttctaa gtacgtgaac
4501 ttcctgtatc tggcaagcca ctacgagaag ctgaaaggat ctccagagga taacgaacag
4561 aaacagctgt ttgtggagca gcacaagcat tatctggacg agatcattga acagattagc
4621 gagttctcta aaagagtgat cctggccgac gcaaatctgg ataaggtcct gtctgcttac
4681 aacaaacaca gagataagcc catcagggag caggccgaaa atatcattca tctgttcact
4741 ctgacaaacc tgggcgcacc tgcagctttc aagtacttcg acactacaat cgatagaaag
4801 aggtacacct ccactaagga ggtgctggac gctacactga tccatcagag tattaccggc
4861 ctgtacgaaa caaggattga cctgtctcag ctgggtggcg actcaccggt gggatcccct
4921 aagaagaaga gaaaggtgag gtctggtggc ggagagggca gaggcagtct gctgacatgc
4981 ggtgacgtgg aagagaatcc cggcccttct agaatggtga gcaagggcga agagctgttc
5041 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
5101 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
5161 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
5221 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
5281 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
5341 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
5401 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
5461 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
5521 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
5581 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
5641 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
5701 atcactctcg gcatggacga gctgtacaag taagcggccg ccaccgcggt ggagctccag
5761 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagcaccc
5821 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
5881 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
5941 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
6001 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
6061 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
6121 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
6181 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
6241 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
6301 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
6361 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
6421 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
6481 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
6541 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
6601 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
6661 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
6721 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
6781 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
6841 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
6901 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
6961 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
7021 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
7081 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
7141 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
7201 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
7261 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
7321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
7381 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
7441 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
7501 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
7561 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
7621 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
7681 tgctc
//
