pHSE-Luc

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pHSE-Luc
质粒类型: 信号通路报告系统载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: --
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号:  #K2049-1
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pHSE-Luc载体图谱



载体简介

pHSE-Luc is designed to monitor the activation of heat shock factor (HSF) and heat shock-mediatedsignal transduction pathways. 
pHSE-Luc contains the firefly luciferase (luc) gene from Photinuspyralis (1). This vector also contains multiple copies of the 
HSE consensus sequence fused to aTATA-like promoter (PTAL) region from the Herpes simplex virus thymidine kinase (HSV-TK)promoter. After endogenous HSF binds the heat-shock element (HSE; 2), transcription is inducedand the reporter gene is activated.
The luciferase coding sequence is followed by the SV40 late polyadenylation signal to ensureproper, efficient processing of the luc transcript in eukaryotic cells. Located upstream of HSE is asynthetic transcription blocker (TB), which is composed of 
adjacent polyadenylation and transcrip-tion pause sites for reducing background transcription (3). The vector backbone also 
contains an f1origin for single-stranded DNA production, a pUC origin of replication, and an ampicillin resistancegene for 
propagation and selection in E. coli.

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