pCLXSN

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品人: Addgene
载体名称: pCLXSN
质粒类型: 逆病毒载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 7819 bp
5' 测序引物及序列: pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3'
3' 测序引物及序列: --
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: DH5α、HB101等
宿主细胞(系): 常规哺乳动物细胞系
备注:

推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: 12343
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pCLXSN载体图谱



pCLXSN多克隆位点

载体简介

载体序列

LOCUS       pCLXSN	7819 bp 	DNA	SYN
DEFINITION  pCLXSN
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="pCLXSN"
                     /mol_type="other DNA"
     promoter        8..582
                     /label="CMV_immearly_promoter"
     misc_feature    72..764
                     /label="5_LTR"
     misc_feature    87..373
                     /label="CAG_enhancer"
     misc_feature    539..559
                     /label="CMV_fwd_primer"
     misc_feature    834..1643
                     /label="psi_plus_pack"
     misc_feature    1230..1637
                     /label="gag"
     misc_feature    1573..1595
                     /label="pLXSN_5_primer"
     misc_feature    1573..1595
                     /label="MSCV_primer"
     misc_feature    1611..1627
                     /label="pBABE_5_primer"
     misc_feature    complement(1685..1705)
                     /label="pBABE_3_primer"
     misc_feature    complement(1691..1906)
                     /label="SV40_enhancer"
     promoter        1703..1971
                     /label="SV40_promoter"
     rep_origin      1870..1947
                     /label="SV40_origin"
     misc_feature    1932..1951
                     /label="SV40pro_F_primer"
     CDS             2079..2873
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            2082..2870
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    2933..3526
                     /label="3MoMuLV_LTR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    2975..3526
                     /label="5_LTR2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    complement(3629..3651)
                     /label="pGEX_3_primer"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    3872..3887
                     /label="SV40_int"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    3893..3940
                     /label="SV40_3_splice"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     terminator      4362..4481
                     /label="SV40_PA_terminator"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    4450..4469
                     /label="EBV_rev_primer"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    5388..5407
                     /label="SV40pro_F_primer"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     rep_origin      complement(5681..6300)
                     /label="pBR322_origin"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(6455..7313)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(6455..7021)
                     /label="ORF frame 1"
                     /translation="MTWLSSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGG
                     PKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLA
                     SRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVV
                     IYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(7355..7383)
                     /label="AmpR_promoter"
                     /translation="MTWLSSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGG
                     PKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLA
                     SRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVV
                     IYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    complement(7542..7564)
                     /label="pGEX_3_primer"
                     /translation="MTWLSSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGG
                     PKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLA
                     SRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVV
                     IYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
    1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
   61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
  121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttccatag taacgccaat
  181 agggactttc cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt
  241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
  301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
  361 cgtattagtc atcgctatta ccatgtgatg cggttttggc agtacatcaa tgggcgtgga
  421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
  481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
  541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcaGagctcA ATAAAAGAGC
  601 CCACAACCCC TCACTCGGCG CGCCAGTCTT CCGATAGACT GCGTCGCCCG GGTACCCGTA
  661 TTCCCAATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG TCTCGCTGTT CCTTGGGAGG
  721 GTCTCCTCTG AGTGATTGAC TACCCACGAC GGGGGTCTTT CATTTGGGGG CTCGTCCGGG
  781 ATTTGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC TGGCCAGCAA
  841 CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGTTTGA TGTTATGCGC CTGCGTCTGT
  901 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA
  961 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA
 1021 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG
 1081 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA
 1141 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG
 1201 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT
 1261 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA
 1321 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG
 1381 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC
 1441 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC
 1501 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG
 1561 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG
 1621 CCCTCACTCC TTCTCTAGGC GCCGGAATTG CGGCCGCAAT TCGTTAACTC GAGGATCCGG
 1681 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
 1741 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA
 1801 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
 1861 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
 1921 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
 1981 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGGGCT GCAGGTCGAG
 2041 GCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
 2101 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
 2161 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
 2221 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
 2281 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
 2341 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
 2401 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
 2461 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
 2521 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
 2581 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
 2641 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
 2701 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
 2761 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
 2821 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
 2881 TCTGGGGTTC GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG
 2941 ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA
 3001 ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT
 3061 ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG
 3121 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
 3181 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC TCAGCAGTTT CTAGAGAACC
 3241 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA
 3301 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA
 3361 GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG
 3421 TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG
 3481 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATTTGGG GGCTCGTCCG
 3541 GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC CGGGAGGTAA GCTGGCTGCC
 3601 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
 3661 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
 3721 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACct
 3781 agctaggtag ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
 3841 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
 3901 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
 3961 aatgggagca gtggtccaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
 4021 ctctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
 4081 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
 4141 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
 4201 gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa
 4261 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
 4321 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
 4381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
 4441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctga tcccgccatg
 4501 gtatcaacgc catatttcta tttacagtag ggacctcttc gttgtgtagg taccgctgta
 4561 ttcctaggga aatagtagag gcaccttgaa ctgtctgcat cagccatata gcccccgctg
 4621 ttcgacttac aaacacaggc acagtactga caaacccata cacctcctct gaaataccca
 4681 tagttgctag ggctgtctcc gaactcatta caccctccaa agtcagagct gtaatttcgc
 4741 catcaagggc agcgagggct tctccagata aaatagcttc tgccgagagt cccgtaaggg
 4801 tagacacttc agctaatccc tcgatgaggt ctactagaat agtcagtgcg gctcccattt
 4861 tgaaaattca cttacttgat cagcttcaga agatggcgga gggcctccaa cacagtaatt
 4921 ttcctcccga ctcttaaaat agaaaatgtc aagtcagtta agcaggaagt ggactaactg
 4981 acgcagctgg ccgtgcgaca tcctctttta attagttgct aggcaacgcc ctccagaggg
 5041 cgtgtggttt tgcaagagga agcaaaagcc tctccaccca ggcctagaat gtttccaccc
 5101 aatcattact atgacaacag ctgttttttt tagtattaag cagaggccgg ggacccctgg
 5161 cccgcttact ctggagaaaa aaaacattgt agaggcttcc agaggcaact tgtcaaaaca
 5221 ggactgcttc tatttctgtc acactgtctg gccctgtcac aaggtccagc acctccatac
 5281 cccctttaat aagcagtttg ggaacgggtg cgggtcttac tccgcccatc cgcccctaac
 5341 tccgcccagt tccgcccatt ctccgcccca tgctgactaa ttttttttat ttatgcagag
 5401 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggC
 5461 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
 5521 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
 5581 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
 5641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
 5701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
 5761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
 5821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
 5881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
 5941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
 6001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
 6061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
 6121 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
 6181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
 6241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
 6301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
 6361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
 6421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
 6481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
 6541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
 6601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
 6661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
 6721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
 6781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
 6841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
 6901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
 6961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgaACTC AACCAAGTCA
 7021 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
 7081 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
 7141 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
 7201 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
 7261 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
 7321 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
 7381 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
 7441 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
 7501 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
 7561 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
 7621 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
 7681 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
 7741 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
 7801 GCCTCTTCGC TATTACGCC
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng