| 出品公司: | Agilent |
|---|---|
| 载体名称: | pCMV-3Tag-6 |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | HSV TK, CMV |
| 载体大小: | 5241 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 3xFLAG |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 哺乳动物表达载体，用于标记具有N-末端3xFLAG表位的蛋白质。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-3Tag-6 5241 bp ds-DNA circular SYN 28-11-2013
DEFINITION Mammalian expression vector with a hygromycin resistance marker for
tagging proteins with an N-terminal 3xFLAG epitope.
ACCESSION .
VERSION .
KEYWORDS pCMV-3Tag-6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5241)
AUTHORS Agilent Technologies
TITLE Direct Submission
JOURNAL Exported 2017/2/28 from SnapGene 2.3.2
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5241
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 66..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 620..638
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 679..681
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 682..753
/codon_start=1
/product="three tandem FLAG epitope tags"
/note="3xFLAG"
/translation="DYKDDDDKDYKDDDDKDYKDDDDK"
misc_feature 754..828
/note="MCS"
/note="multiple cloning site"
misc_feature 836..846
/note="stop codons"
/note="stop codons in all three reading frames"
promoter complement(874..892)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 927..1134
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(1141..1596)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1623..1727
/gene="bla"
/note="AmpR promoter"
CDS 1728..2585
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter 2679..2824
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
CDS 2867..3904
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN"
misc_feature 3946..3994
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4545..5133
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataaggacta taaggacgat
721 gatgacaagg actacaaaga tgatgacgat aaagcccggg cgggatcccc cgggctgcag
781 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccttaatt
841 aattaaggta ccaggtaagt gtacccaatt cgccctatag tgagtcgtat tacaattcac
901 tcgatcggct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
961 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
1021 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
1081 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggaacgcgt
1141 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
1201 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
1261 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
1321 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
1381 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
1441 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
1501 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
1561 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
1621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
1681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
1741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
1801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
1861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
1921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
1981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
2041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
2101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
2161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
2221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
2281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
2341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
2401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
2461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
2521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
2581 attggcctca ggattagtgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
2641 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg
2701 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca
2761 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc
2821 ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga
2881 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
2941 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
3001 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
3061 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
3121 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
3181 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
3241 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
3301 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
3361 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
3421 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
3481 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
3541 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
3601 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
3661 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
3721 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
3781 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
3841 cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa
3901 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
3961 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
4021 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
4081 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
4141 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga
4201 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact
4261 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
4321 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
4381 gcggatttct ggcgccgccg gacgaactaa acctgactac gacatctctg ccccttcttc
4441 gctggtacga ggagcgcttt gcctcaggtc atgaccaaaa tcccttaacg tgagttttcg
4501 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
4561 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
4621 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
4681 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
4741 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
4801 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
4861 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
4921 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
4981 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
5041 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
5101 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
5161 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
5221 gtggataacc gtattaccgc c
//
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