pLKO.1-blast

价格:1200元

联系方式:I47-825O-882O

买家导航

载体基本信息

质粒名称: pLKO.1-blast 
出品公司: Addgene
目录编号: 26655
存储实验室: Keith Mostov

质粒骨架基本信息
质粒名称: pLKO.1
质粒出品公司: David Root
质粒抗性: 氨苄霉素
空质粒大小: 7000 bp
质粒类型: 哺乳细胞表达
筛选标记: 稻瘟
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
克隆方法: 限制性内切酶
5' 克隆位点: AgeI(未破坏)
3' 克隆位点: EcoRI(未破坏)
5' 测序引物 LKO.1 5'
3' 测序引物  

文献引用方法
材料和方法部分: pLKO.1-blast was a gift from Keith Mostov (Addgene plasmid # 26655)
参考文献部分: A molecular network for de novo generation of the apical surface and lumen. Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, Martin-Belmonte F, Mostov KE. Nat Cell Biol. 2010 Oct 3. ():. 10.1038/ncb2106 PubMed 20890297

载体质粒图谱和多克隆位点信息

pLKO.1-blast载体图谱

载体简介

载体序列

LOCUS       pLKO.1-blast Sequencing Result	6783 bp 	DNA	SYN
DEFINITION  pLKO.1-blast Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="pLKO.1-blast Sequencing Result"
                     /mol_type="other DNA"
     gene            519..917
                     /label="blast"
                     /gene="blast"
     misc_feature    972..993
                     /label="U3PPT"
     misc_feature    972..987
                     /label="cPPT"
     misc_feature    989..1041
                     /label="delta_U3"
     misc_feature    1042..1222
                     /label="HIV-1_5_LTR"
     misc_feature    1042..1222
                     /label="truncHIV-1_3_LTR"
     terminator      1297..1416
                     /label="SV40_PA_terminator"
     misc_feature    1385..1404
                     /label="EBV_rev_primer"
     rep_origin      1434..1532
                     /label="SV40_origin"
     misc_feature    1517..1536
                     /label="SV40pro_F_primer"
     promoter        complement(1611..1629)
                     /label="T7_promoter"
     promoter        complement(1639..1655)
                     /label="M13_forward20_primer"
     misc_feature    complement(1648..1670)
                     /label="M13_pUC_fwd_primer"
     misc_feature    1648..1796
                     /label="lacZ_a"
     rep_origin      1814..2120
                     /label="f1_origin"
     promoter        2313..2341
                     /label="AmpR_promoter"
     gene            2383..3243
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2383..3243
                     /label="ORF frame 1"
     rep_origin      3398..4017
                     /label="pBR322_origin"
     promoter        4326..4355
                     /label="lac_promoter"
     misc_feature    4369..4391
                     /label="M13_pUC_rev_primer"
     promoter        4390..4408
                     /label="M13_reverse_primer"
     promoter        4425..4444
                     /label="T3_promoter"
     misc_feature    4698..4878
                     /label="truncHIV-1_3_LTR"
     misc_feature    4698..4878
                     /label="HIV-1_5_LTR"
     misc_feature    4989..5033
                     /label="HIV-1_psi_pack"
     CDS             5421..6278
                     /label="ORF frame 3"
     misc_feature    5543..5776
                     /label="RRE"
     promoter        6312..6544
                     /label="hU6_promoter"
     misc_feature    6474..6493
                     /label="LKO1_5_primer"
     misc_feature    6618..6633
                     /label="cPPT"
     CDS             complement(6699..821)
                     /label="ORF frame 2"
ORIGIN
    1 TTGGGGTTGC GCCTTTTCCA AGGCAGCCCT GGGTTTGCGC AGGGACGCGG CTGCTCTGGG
   61 CGTGGTTCCG GGAAACGCAG CGGCGCCGAC CCTGGGTCTC GCACATTCTT CACGTCCGTT
  121 CGCAGCGTCA CCCGGATCTT CGCCGCTACC CTTGTGGGCC CCCCGGCGAC GCTTCCTGCT
  181 CCGCCCCTAA GTCGGGAAGG TTCCTTGCGG TTCGCGGCGT GCCGGACGTG ACAAACGGAA
  241 GCCGCACGTC TCACTAGTAC CCTCGCAGAC GGACAGCGCC AGGGAGCAAT GGCAGCGCGC
  301 CGACCGCGAT GGGCTGTGGC CAATAGCGGC TGCTCAGCAG GGCGCGCCGA GAGCAGCGGC
  361 CGGGAAGGGG CGGTGCGGGA GGCGGGGTGT GGGGCGGTAG TGTGGGCCCT GTTCCTGCCC
  421 GCGCGGTGTT CCGCATTCTG CAAGCCTCCG GAGCGCACGT CGGCAGTCGG CTCCCTCGTT
  481 GACCGAATCA CCGACCTCTC TCCCCAGGGG GATCCACCAT GGCCAAGCCT TTGTCTCAAG
  541 AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC ATCTCTGAAG
  601 ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT GGTGTCAATG
  661 TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT GCTGGGCACT GCTGCTGCTG
  721 CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG GGCATCTTGA
  781 GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA TCCTGGGATC AAAGCCATAG
  841 TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG CCCTCTGGTT
  901 ATGTGTGGGA GGGCTAAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT
  961 AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA
 1021 GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
 1081 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
 1141 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
 1201 TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA
 1261 CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT
 1321 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT
 1381 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC
 1441 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG
 1501 GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC
 1561 AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGGACGTA CCCAATTCGC CCTATAGTGA
 1621 GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
 1681 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
 1741 AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGACGC
 1801 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC
 1861 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT
 1921 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC
 1981 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC
 2041 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT
 2101 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG
 2161 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC
 2221 GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG AAATGTGCGC
 2281 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
 2341 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC
 2401 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA
 2461 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA
 2521 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG
 2581 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA
 2641 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
 2701 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC
 2761 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA
 2821 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG
 2881 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA
 2941 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
 3001 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
 3061 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
 3121 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA
 3181 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
 3241 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
 3301 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
 3361 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
 3421 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
 3481 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
 3541 GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
 3601 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
 3661 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
 3721 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
 3781 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
 3841 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
 3901 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
 3961 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
 4021 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
 4081 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC
 4141 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGagcgccc aatacgcaaa
 4201 ccgcctctcc ccgcgcgttg gccgattcat taatgCAGCT GGCACGACAG GTTTCCCGAC
 4261 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC
 4321 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA
 4381 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA
 4441 AGGGAACAAA AGCTGGAGCT GCAAGCTTAA TGTAGTCTTA TGCAATACTC TTGTAGTCTT
 4501 GCAACATGGT AACGATGAGT TAGCAACATG CCTTACAAGG AGAGAAAAAG CACCGTGCAT
 4561 GCCGATTGGT GGAAGTAAGG TGGTACGATC GTGCCTTATT AGGAAGGCAA CAGACGGGTC
 4621 TGACATGGAT TGGACGAACC ACTGAATTGC CGCATTGCAG AGATATTGTA TTTAAGTGCC
 4681 TAGCTCGATA CATAAACGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG
 4741 GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG
 4801 TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG
 4861 TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG GGAAACCAGA
 4921 GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG GCGAGGGGCG
 4981 GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA GAGATGGGTG
 5041 CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA TTCGGTTAAG
 5101 GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA GGGAGCTAGA
 5161 ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC AAATACTGGG
 5221 ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT
 5281 AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA
 5341 CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG CCGCTGATCT
 5401 TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG
 5461 TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA
 5521 GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA
 5581 GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA
 5641 TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC
 5701 TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA
 5761 AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG
 5821 TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT
 5881 GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG
 5941 AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA
 6001 GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA
 6061 TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG
 6121 TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG
 6181 ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT
 6241 TAGTGAACGG ATCTCGACGG TATCGATCAC GAGACTAGCC TCGAGCGGCC GCCCCCTTCA
 6301 CCGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG
 6361 AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA
 6421 GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC
 6481 ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG
 6541 GACGAAACAC CGGTCCGCAG GTATGCACGC GTGAATTCTC GACCTCGAGA CAAATGGCAG
 6601 TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA
 6661 TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA
 6721 TTCAAAATTT TCGGGTTTAT TACAGGGACA GCAGAGATCC ACTTTGGCCG CGGCTCGAGG
 6781 GGG
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng