| 出品公司: | Addgene |
|---|---|
| 载体名称: | PX385 或 PX385 Streptococcus pasteurianus Cas9 |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 10586 bp |
| 5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
| 载体标签: | NLS (C ter)；HA、NLS (N ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 昆虫细胞 |
| 备注: | -- |
| 产品目录号: | #68322 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PX385 Streptococcus pasteurianus Cas9 10586 bp DNA SYN
DEFINITION PX385 Streptococcus pasteurianus Cas9
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10586
/organism="PX385 Streptococcus pasteurianus Cas9"
/mol_type="other DNA"
misc_feature 47..1001
/label="Ac_5_flank"
CDS complement(343..948)
/label="ORF frame 3"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1023..1587
/label="CMV_immearly_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1090..1377
/label="CAG_enhancer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1544..1564
/label="CMV_fwd_primer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1615..1633
/label="T7_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1633..1656
/label="lacO"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1772..1796
/label="attB1"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
CDS 1822..5277
/label="ORF frame 1"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature 1825..1851
/label="HA"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature 1855..1875
/label="SV40 NLS"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature 1861..1875
/label="NLS"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature complement(5310..5327)
/label="Sp6_primer"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature 5332..5356
/label="attB2"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
terminator 5368..5834
/label="hGH_PA_terminator"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
terminator 5840..5875
/label="T7_terminator"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
rep_origin 5992..6298
/label="f1_origin"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature complement(6412..6432)
/label="pBABE_3_primer"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature complement(6418..6633)
/label="SV40_enhancer"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
promoter 6430..6698
/label="SV40_promoter"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
rep_origin 6597..6674
/label="SV40_origin"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
misc_feature 6659..6678
/label="SV40pro_F_primer"
/translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
KNNKK*"
CDS 6813..7607
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 6816..7604
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(7122..7658)
/label="ORF frame 1"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 7784..7903
/label="SV40_PA_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 7872..7891
/label="EBV_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 8094..8129
/label="T7_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature complement(8152..8169)
/label="Baculovirs_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 8159..8236
/label="3_AcPH"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS complement(8266..8883)
/label="ORF frame 3"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
misc_feature 8863..8896
/label="loxP"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
gene complement(9664..10524)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
CDS complement(9664..10524)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatgact aacggcaaga ttctggggct ggacattggc
1921 atcgcaagcg tgggggtggg gattattgag gcaaaaactg gaaaggtggt gcatgccaat
1981 tcccggctgt tctctgccgc taacgctgag aacaatgcag aacggagagg gtttagggga
2041 tctaggcgcc tgaatcgacg gaagaaacac cgcgtgaagc gagtccggga tctgttcgag
2101 aaatacggaa tcgtcaccga ctttcgcaac ctgaatctga acccttatga gctgcgagtg
2161 aagggcctga ccgaacagct gaaaaacgag gaactgttcg cagccctgag aacaatctct
2221 aagagaaggg ggattagtta cctggacgat gccgaggacg atagtaccgg atcaacagac
2281 tatgctaaga gcatcgatga gaatcgccga ctgctgaaaa acaagacacc aggccagatt
2341 cagctggaga ggctggaaaa gtacggccag ctgcgcggga atttcaccgt ctatgacgag
2401 aacggggaag cccatcgcct gatcaatgtg tttagtacat cagattacga gaaagaagca
2461 cggaagatcc tggagacaca ggccgactac aacaagaaaa tcacagctga gttcattgac
2521 gattatgtgg aaatcctgac ccagaaacga aagtactatc acggccccgg gaacgaaaag
2581 agccggactg actacggacg gttccggacc gatgggacca cactggagaa tattttcgga
2641 atcctgattg gcaagtgcaa cttttaccct gatgaatatc gagcaagcaa ggccagctac
2701 accgcacagg agtataattt cctgaacgac ctgaacaatc tgaaggtgag caccgaaaca
2761 gggaagctgt caacagagca gaaagaaagc ctggtggagt ttgccaagaa tactgctacc
2821 ctgggacccg ctaaactgct gaaggagatc gcaaaaattc tggactgtaa ggtggatgag
2881 atcaaaggat acagagagga cgataaaggc aagccagatc tgcatacctt cgagccctat
2941 aggaaactga agtttaatct ggaaagcatc aacattgacg atctgtcccg cgaagtgatc
3001 gacaagctgg ctgatattct gactctgaac accgagagag aaggaatcga ggacgcaatt
3061 aagaggaatc tgccaaacca gttcacagag gaacagatca gcgagatcat caaggtgcgg
3121 aagagccagt ccactgcttt caataagggc tggcactctt ttagtgcaaa actgatgaac
3181 gagctgatcc ccgaactgta cgccacctcc gacgagcaga tgacaattct gactcggctg
3241 gaaaaattca aggtcaataa gaaaagctcc aaaaacacaa agactatcga cgagaaggaa
3301 gtcactgatg agatctacaa tcctgtggtc gccaagagcg tgagacagac catcaaaatc
3361 attaacgctg cagtcaagaa atatggcgac ttcgataaga tcgtgattga aatgccacgg
3421 gataaaaatg ctgacgatga gaagaagttc atcgacaaga gaaataagga gaacaagaag
3481 gaaaaggacg atgccctgaa aagggccgct tacctgtata attctagtga caagctgccc
3541 gatgaggtgt tccacggcaa caagcagctg gaaaccaaaa tccgactgtg gtatcagcag
3601 ggggagcggt gcctgtatag tggaaagccc atctcaattc aggagctggt gcataactct
3661 aacaatttcg aaatcgatca cattctgcct ctgtcactga gctttgacga tagtctggcc
3721 aataaggtgc tggtctacgc ttggacaaac caggagaaag gccagaaaac cccttatcag
3781 gtcatcgact ccatggatgc agcctggtct ttcagggaga tgaaggacta cgtgctgaaa
3841 cagaagggac tgggcaagaa aaagcgcgac tatctgctga ctaccgagaa catcgataag
3901 attgaagtga agaagaagtt catcgagagg aatctggtgg atactcgcta cgcatctcga
3961 gtggtcctga actctctgca gagtgccctg agagagctgg ggaaagacac taaggtgtct
4021 gtggtcaggg gacagttcac cagtcagctg cggagaaaat ggaagatcga taagagccgc
4081 gagacatacc accatcacgc agtggacgcc ctgatcattg ctgcatcaag ccagctgaaa
4141 ctgtgggaga agcaggacaa tcccatgttt gtggattatg gcaagaacca ggtggtcgac
4201 aaacagactg gggagatcct gtccgtgtct gacgatgagt acaaggaact ggtgttccag
4261 cccccttatc agggctttgt gaataccatc tcctctaaag ggttcgagga cgaaattctg
4321 tttagctacc aggtggattc caaatataac cggaaggtca gtgacgcaac catctactca
4381 acaagaaaag ccaagattgg caaggataag aaagaggaaa cctacgtgct gggaaaaatc
4441 aaggacatct actcccagaa tggcttcgat accttcatca agaagtacaa caaagataag
4501 actcagttcc tgatgtatca gaaggactct ctgacatggg agaacgtgat cgaagtcatt
4561 ctgagggact acccaacaac taagaaaagc gaggacggca aaaatgatgt gaagtgcaac
4621 ccctttgagg aatacaggcg cgagaatggg ctgatctgta agtattccaa gaaagggaaa
4681 ggaactccca tcaagagcct gaagtactat gacaagaaac tggggaactg catcgatatt
4741 accccagagg aatcacgcaa taaggtcatc ctgcagagca ttaacccttg gcgagccgac
4801 gtgtacttca atccagagac actgaagtac gaactgatgg gcctgaaata ttcagatctg
4861 agctttgaaa agggcactgg gaactaccat atcagccagg agaaatatga cgctatcaaa
4921 gagaaggaag gaattggcaa gaaatccgag ttcaagttta cactgtaccg caacgacctg
4981 atcctgatca aggatatcgc cagtggcgag caggaaatct acagattcct gtcaagaact
5041 atgcccaatg tgaaccacta cgtcgagctg aagccttacg acaaggaaaa gttcgataac
5101 gtgcaggagc tggtcgaagc actgggagag gcagataaag tgggacgatg tatcaaagga
5161 ctgaataagc caaacatcag catctacaag gtgagaaccg acgtcctggg aaacaaatat
5221 ttcgtgaaga aaaagggcga caaacccaag ctggatttta agaacaacaa gaagtaagaa
5281 ttcggtaccg agctcaagct tgcggccgcc tatagtgtca cctaaatcgt aacccagctt
5341 tcttgtacaa agtggtcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
5401 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
5461 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
5521 ggcaagttgg gatgacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
5581 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
5641 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
5701 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
5761 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
5821 ccttccctgt ccttctagca taaccccttg gggcctctaa acgggtcttg aggggttttt
5881 tgtctagaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
5941 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
6001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
6061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
6121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
6181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
6241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
6301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
6361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
6421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
6481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
6541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
6601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
6661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
6721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
6781 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
6841 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
6901 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
6961 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
7021 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
7081 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
7141 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
7201 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
7261 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
7321 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
7381 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
7441 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
7501 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
7561 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
7621 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
7681 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
7741 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
7801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gttcatgaag
7921 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
7981 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
8041 cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact agcataaccc
8101 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct
8161 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
8221 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
8281 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
8341 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
8401 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
8461 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
8521 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
8581 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
8641 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg
8701 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
8761 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
8821 cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata
8881 cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt
8941 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
9001 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
9061 gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
9241 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
9481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
9541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
9601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
9661 ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
9721 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
9781 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
9841 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
9901 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
9961 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
10021 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
10081 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
10141 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
10201 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
10261 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
10321 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
10381 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
10441 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
10501 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
10561 gggttattgt ctcatgtccg cgcgtt
//
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