pMX57

LOCUS       pMX57           4779 bp DNA     circular SYN 02-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..14
                     /label=NFAT_IL4
                     /label=nonstandard type:  RE
     misc_feature    20..37
                     /label=NFAT_IL4
                     /label=nonstandard type:  RE
     misc_feature    50..67
                     /label=NFAT_IL4
                     /label=nonstandard type:  RE
     misc_feature    80..97
                     /label=NFAT_IL4
                     /label=nonstandard type:  RE
     promoter        111..142
                     /label=P_min
     CDS             182..1741
                     /codon_start=1
                     /label=SEAP (Synthetic Clontech)
                     /label=nonstandard type:  reporter
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPGYSRVGAAGRFE
                     QT"
     primer_bind     complement(230..248)
                     /label=175-WKG-587-R
     polyA_signal    1742..1863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2100..3040
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(3041..3901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3902..4006)
                     /label=AmpR promoter
     rep_origin      4033..4488
                     /label=M13 origin
     CDS             complement(4490..4558)
                     /label=LacZ alpha
     misc_feature    4619..4772
                     /label=Transcription blocker
                     /label=Transcription\blocker
     primer_bind     4671..4694
                     /label=174-WKG-587-F
ORIGIN
        1 ggtacctgag ctcgctagct acattggaaa attttataca cgttctagct acattggaaa
       61 attttataca cgttctagct acattggaaa attttataca cgttagactc tagagggtat
      121 ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaaga attcgcccac
      181 catgctgctg ctgctgctgc tgctgggcct gaggctacag ctctccctgg gcatcatccc
      241 agttgaggag gagaacccgg acttctggaa ccgcgaggca gccgaggccc tgggtgccgc
      301 caagaagctg cagcctgcac agacagccgc caagaacctc atcatcttcc tgggcgatgg
      361 gatgggggtg tctacggtga cagctgccag gatcctaaaa gggcagaaga aggacaaact
      421 ggggcctgag atacccctgg ccatggaccg cttcccatat gtggctctgt ccaagacata
      481 caatgtagac aaacatgtgc cagacagtgg agccacagcc acggcctacc tgtgcggggt
      541 caagggcaac ttccagacca ttggcttgag tgcagccgcc cgctttaacc agtgcaacac
      601 gacacgcggc aacgaggtca tctccgtgat gaatcgggcc aagaaagcag ggaagtcagt
      661 gggagtggta accaccacac gagtgcagca cgcctcgcca gccggcacct acgcccacac
      721 ggtgaaccgc aactggtact cggacgccga cgtgcctgcc tcggcccgcc aggaggggtg
      781 ccaggacatc gctacgcagc tcatctccaa catggacatt gacgtgatcc taggtggagg
      841 ccgaaagtac atgtttcgca tgggaacccc agaccctgag tacccagatg actacagcca
      901 aggtgggacc aggctggacg ggaagaatct ggtgcaggaa tggctggcga agcgccaggg
      961 tgcccggtat gtgtggaacc gcactgagct catgcaggct tccctggacc cgtctgtgac
     1021 ccatctcatg ggtctctttg agcctggaga catgaaatac gagatccacc gagactccac
     1081 actggacccc tccctgatgg agatgacaga ggctgccctg cgcctgctga gcaggaaccc
     1141 ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac catggtcatc atgaaagcag
     1201 ggcttaccgg gcactgactg agacgatcat gttcgacgac gccattgaga gggcgggcca
     1261 gctcaccagc gaggaggaca cgctgagcct cgtcactgcc gaccactccc acgtcttctc
     1321 cttcggaggc taccccctgc gagggagctc catcttcggg ctggcccctg gcaaggcccg
     1381 ggacaggaag gcctacacgg tcctcctata cggaaacggt ccaggctatg tgctcaagga
     1441 cggcgcccgg ccggatgtta ccgagagcga gagcgggagc cccgagtatc ggcagcagtc
     1501 agcagtgccc ctggacgaag agacccacgc aggcgaggac gtggcggtgt tcgcgcgcgg
     1561 cccgcaggcg cacctggttc acggcgtgca ggagcagacc ttcatagcgc acgtcatggc
     1621 cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg gcgccccccg ccggcaccac
     1681 cgacgccgcg cacccgggtt actctagagt cggggcggcc ggccgcttcg agcagacatg
     1741 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1801 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1861 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1921 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat
     1981 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     2041 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     2101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2701 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2941 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3001 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3061 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3121 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3181 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3241 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3301 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3361 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3421 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3481 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3541 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3601 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3661 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3721 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3781 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3841 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3901 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3961 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4021 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4081 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4141 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4201 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4261 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gaatccacgt
     4321 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4381 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4441 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc cattcgccat
     4501 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     4561 ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga gcggccgcaa
     4621 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     4681 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     4741 cagtgcaagt gcaggtgcca gaacatttct ctatcgata
//