| 质粒名称: | pHDM-Hgpm2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 164441 |
| 存储实验室: | Alejandro Balazs |
|
载体骨架基本信息 |
|
| 载体名称: | HDM |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素,100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Codon Optimized HIV-1 GagPol |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHDM-Hgpm2 was a gift from Alejandro Balazs (Addgene plasmid # 164441 ; http://n2t.net/addgene:164441 ; RRID:Addgene_164441) |
| 参考文献部分: | COVID-19-neutralizing antibodies predict disease severity and survival. Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG, Iafrate AJ, Balazs AB. Cell. 2021 Jan 21;184(2):476-488.e11. doi: 10.1016/j.cell.2020.12.015. Epub 2020 Dec 15. 10.1016/j.cell.2020.12.015 PubMed 33412089 |
买家导航
pHDMHgpm2载体基本信息
pHDMHgpm2载体图谱质粒图谱和多克隆位点信息
pHDMHgpm2载体简介
pHDMHgpm2载体序列
LOCUS pHDM-Hgpm2 8910 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Encodes codon-optimized HIV gagpol for pseudovirus production.
ACCESSION .
VERSION .
KEYWORDS pHDM-Hgpm2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8910)
TITLE COVID-19 neutralizing antibodies predict disease severity and
survival
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8910)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8910
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 114..589
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
primer_bind complement(5015..5031)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5016..5032)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 5125..5519
/label=-beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
primer_bind complement(5853..5871)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5939..6043
/gene="bla"
/label=AmpR promoter
CDS 6044..6904
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6262..6281)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7075..7663
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7564..7583
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(8206..8225)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
enhancer 8327..8706
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8707..8910
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8861..8881
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 8907..21
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
61 agacaccggg accgatccag cctcccctcg aagctgatcc tgagaacttc agggtgagtc
121 tatgggaccc ttgatgtttt ctttcccctt cttttctatg gttaagttca tgtcatagga
181 aggggagaag taacagggta cacatattga ccaaatcagg gtaattttgc atttgtaatt
241 ttaaaaaatg ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc
301 ctaatctctt tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct
361 aaagaataac agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat
421 ttctgcatat aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc
481 cagctaccat tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag
541 ctaggccctt ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa
601 cgtgctggtc tgtgtgctgg cccatcactt tggcaaagaa ttctagactg ccatgggcgc
661 ccgcgcctcc gtgctgtccg gcggcgagct ggacaagtgg gagaagatcc gcctgcgccc
721 cggcggcaag aagcagtaca agctgaagca catcgtgtgg gcctcccgcg agctggagcg
781 cttcgccgtg aaccccggcc tgctggagac ctccgagggc tgccgccaga tcctgggcca
841 gctgcagccc tccctgcaaa ccggctccga ggagctgcgc tccctgtaca acaccatcgc
901 cgtgctgtac tgcgtgcacc agcgcatcga cgtgaaggac accaaggagg ccctggacaa
961 gatcgaggag gagcagaaca agtccaagaa gaaggcccag caggccgccg ccgacaccgg
1021 caacaactcc caggtgtccc agaactaccc catcgtgcag aacctgcagg gccagatggt
1081 gcaccaggcc atctcccccc gcaccctgaa cgcctgggtg aaggtggtgg aggagaaggc
1141 cttctccccc gaagtcatcc ccatgttctc cgccctgtcc gagggcgcca ccccccagga
1201 cctgaacacc atgctgaaca ccgtgggcgg ccaccaggcc gccatgcaga tgctgaagga
1261 gaccatcaac gaggaggccg ccgagtggga ccgcctgcac cccgtgcacg ccggccccat
1321 cgcccccggc cagatgcgcg agccccgcgg ctccgacatc gccggcacca cctccaccct
1381 gcaagagcag atcggctgga tgacccacaa cccccccatc cccgtgggcg agatctacaa
1441 gcgctggatc atcctgggcc tgaacaagat cgtgcgcatg tactccccca cctccatcct
1501 ggacatccgc cagggcccca aggagccctt ccgcgactac gtggaccgct tctacaagac
1561 cctgcgcgcc gagcaggcct cccaggaggt aaagaactgg atgaccgaga ccctgctggt
1621 gcagaacgcc aaccccgact gcaagaccat cctgaaggcc ctgggccccg gcgccaccct
1681 ggaggagatg atgaccgcct gccagggcgt gggcggcccc ggccacaagg cccgcgtgct
1741 ggccgaggcc atgtcccaag tcaccaaccc cgccaccatc atgatccaga agggcaactt
1801 ccgcaaccag cgcaagaccg tgaagtgctt caactgcggc aaggagggcc acatcgccaa
1861 gaactgccgc gccccccgca agaagggctg ctggaagtgc ggcaaggagg gccaccagat
1921 gaaagattgt actgagagac aggctaattt tttagggaag atctggcctt cccacaaggg
1981 aaggccaggg aattttcttc agagcagacc agagccaaca gccccaccag aagagagctt
2041 caggtttggg gaagagacaa caactccctc tcagaagcag gagccgatag acaaggaact
2101 gtatccttta gcttccctca gatcactctt tggcagcgac ccctcgtcac aataaagatc
2161 ggtggccagc tgaaggaggc cctgctggac accggcgccg acgacaccgt gctggaggag
2221 atgaacctgc ccggccgctg gaagcccaag atgatcggcg gcatcggcgg cttcatcaaa
2281 gtccgccagt acgaccagat cctgatcgag atctgcggcc acaaggccat cggcaccgtg
2341 ctggtgggcc ccacccccgt gaacatcatc ggccgcaacc tgctgaccca gatcggctgc
2401 accctgaact tccccatctc ccccatcgag accgtgcccg tgaagctgaa gcccggcatg
2461 gacggcccca aagtcaagca gtggcccctg accgaggaga agatcaaggc cctggtggag
2521 atctgcaccg agatggagaa ggagggcaag atctccaaga tcggccccga gaacccctac
2581 aacacccccg tgttcgccat caagaagaag gactccacca agtggcgcaa gctggtggac
2641 ttccgcgagc tgaacaagcg cacccaggac ttctgggagg tgcagctggg catcccccac
2701 cccgccggcc tgaagcagaa gaagtccgtg accgtgctgg acgtgggcga cgcctacttc
2761 tccgtgcccc tggacaagga cttccgcaag tacaccgcct tcaccatccc ctccatcaac
2821 aacgagaccc ccggcatccg ctaccagtac aacgtgctgc cccagggctg gaagggctcc
2881 cccgccatct tccagtgctc catgaccaag atcctggagc ccttccgcaa gcagaacccc
2941 gacatcgtga tctaccagta catggacgac ctgtacgtgg gctccgacct ggagatcggc
3001 cagcaccgca ccaagatcga ggagctgcgc cagcacctgc tgcgctgggg cttcaccacc
3061 cccgacaaga agcaccagaa ggagcccccc ttcctgtgga tgggctacga gctgcacccc
3121 gacaagtgga ccgtgcagcc catcgtgctg cccgagaagg actcctggac cgtgaacgac
3181 atccagaagc tggtgggcaa gctgaactgg gcctcccaga tctacgccgg catcaaagtc
3241 cgccagctgt gcaagctgct gcgcggcacc aaggccctga ccgaggtggt gcccctgacc
3301 gaggaggccg agctggagct ggccgagaac cgcgagatcc tgaaggagcc cgtgcacggc
3361 gtgtactacg acccctccaa ggacctgatc gccgagatcc agaagcaggg ccagggccag
3421 tggacctacc agatctacca ggagcccttc aagaacctga agaccggcaa atacgcccgc
3481 atgaagggcg cccacaccaa cgacgtgaag cagctgaccg aggccgtgca gaagatcgcc
3541 accgagtcca tcgtgatctg gggcaagact cccaagttca agctgcccat ccagaaggag
3601 acctgggagg cctggtggac cgagtactgg caggccacct ggatccccga gtgggagttc
3661 gtgaacaccc cccccctggt gaagctgtgg taccagctgg agaaggagcc catcatcggc
3721 gccgagacct tctacgtgga cggcgccgcc aaccgcgaga ccaagctggg caaggccggc
3781 tacgtgaccg accgcggccg ccagaaggtg gtgcccctga ccgacaccac caaccagaag
3841 accgagctgc aggccatcca cctggccctg caagactccg gcctggaggt gaacatcgtg
3901 accgactccc agtatgcatt gggcatcatc caggcccagc ccgacaagtc cgagtccgag
3961 ctggtgtccc agatcatcga gcagctgatc aagaaggaga aggtgtacct ggcctgggtg
4021 cccgcccaca agggcatcgg cggcaacgag caggtggaca agctggtgtc cgccggcatc
4081 cgcaaggtgc tgttcctgga cggcatcgac aaggcccagg aggagcacga gaagtaccac
4141 tccaactggc gcgccatggc ctccgacttc aacctgcccc ccgtggtggc caaggagatc
4201 gtggcctcct gcgacaagtg ccagctgaag ggcgaggcca tgcacggcca ggtggactgc
4261 tcccccggca tctggcagct ggactgcacc cacctggagg gcaaggtgat cctggtggcc
4321 gtgcacgtgg cctccggcta catcgaggcc gaggtgatcc ccgccgagac cggccaggag
4381 accgcctact tcctgctgaa gctggccggc cgctggcccg tgaagaccgt gcacaccgac
4441 aacggctcca acttcacctc caccaccgtg aaggccgcct gctggtgggc cggcatcaag
4501 caggagttcg gcatccccta caacccccag tcccagggcg tgatcgagtc catgaacaag
4561 gagctgaaga agatcatcgg ccaagtccgc gaccaggccg agcacctgaa gaccgccgtg
4621 cagatggccg tgttcatcca caacttcaag cgcaagggcg gcatcggcgg ctactccgcc
4681 ggcgagcgca tcgtggacat catcgccacc gacatccaga ccaaggagct gcagaagcag
4741 atcaccaaga tccagaactt ccgcgtgtac taccgcgact cccgcgaccc cgtgtggaag
4801 ggccccgcca agctgctgtg gaagggcgag ggcgccgtgg tgatccagga caactccgac
4861 atcaaggtgg tgccccgccg caaggccaag atcatccgcg actacggcaa gcagatggcc
4921 ggcgacgact gcgtggcctc ccgccaggac gaggactaac acatggaaaa gattagtaaa
4981 acaccatagg ccgctctaga ggatccaagc ttatcgatac cgtcgacctc gagggcccag
5041 atctaattca ccccaccagt gcaggctgcc tatcagaaag tggtggctgg tgtggctaat
5101 gccctggccc acaagtatca ctaagctcgc tttcttgctg tccaatttct attaaaggtt
5161 cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct
5221 ggattctgcc taataaaaaa catttatttt cattgcaatg atgtatttaa attatttctg
5281 aatattttac taaaaaggga atgtgggagg tcagtgcatt taaaacataa agaaatgaag
5341 agctagttca aaccttggga aaatacacta tatcttaaac tccatgaaag aaggtgaggc
5401 tgcaaacagc taatgcacat tggcaacagc ccctgatgcc tatgccttat tcatccctca
5461 gaaaaggatt caagtagagg cttgatttgg aggttaaagt tttgctatgc tgtattttac
5521 attacttatt gttttagctg tcctcatgaa tgtcttttca ctacccattt gcttatcctg
5581 catctctcag ccttgactcc actcagttct cttgcttaga gataccacct ttcccctgaa
5641 gtgttccttc catgttttac ggcgagatgg tttctcctcg cctggccact cagccttagt
5701 tgtctctgtt gtcttataga ggtctacttg aagaaggaaa aacagggggc atggtttgac
5761 tgtcctgtga gcccttcttc cctgcctccc ccactcacag tgacccggaa tccctcgaca
5821 tggcagtcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
5881 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
5941 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
6001 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
6061 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
6121 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
6181 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
6241 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
6301 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
6361 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
6421 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
6481 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
6541 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
6601 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
6661 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
6721 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
6781 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
6841 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
6901 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
6961 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
7021 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
7081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
7141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
7201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
7261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
7321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
7381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
7441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
7501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
7561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
7621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacggaga
7681 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
7741 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
7801 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
7861 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
7921 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
7981 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
8041 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
8101 aatggggaag gccatccagc ctcgcgtcgg ggagcttttt gcaaaagcct aggcctccaa
8161 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
8221 aaataaaaaa aattagtcag ccatgagctt ggcccattgc atacgttgta tccatatcat
8281 aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg
8341 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
8401 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
8461 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
8521 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
8581 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
8641 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
8701 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
8761 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
8821 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
8881 gtacggtggg aggtctatat aagcagagct
//
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