pYES2-NTA

价格:1000元

联系方式:I47-825O-882O

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载体基本信息


出品公司: Invitrogen
载体名称: pYES2-NTA, pYES2/NT A
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6038 bp
5' 测序引物及序列: T7: TAATACGACTCACTATAGGG
3' 测序引物及序列: CYC1 Terminator: GTGACATAACTAATTACATGATG
载体标签: N-His, N-Xpress, C-His, C-V5
载体抗性: 氨苄
筛选标记: URA3
备注: 利用半乳糖诱导蛋白在酿酒酵母中表达。
产品目录号: V8252-20
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYES2-NTA载体图谱

pYES2-NTA多克隆位点

载体简介

pYES2 NT A, B, C的是一个6.0 kb的载体,设计用来在酿酒酵母(Saccharomyces cerevisiae)中诱导表达重组蛋白。

该载体包含以下元素:

1. 酵母GAL1启动子,能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白,同时能够被葡萄糖抑制表达

2.多克隆位点可以使用的很多限制酶切位点,便于基因插入。

3.CYC1终止子能够有效终止mRNA的转录。

4.能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。

5.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。

6. 载体本身带有N端His和Xpress标签,同时载体C端也带有V5抗原表位和His纯化标签。

8. 在酵母中,利用载体上的2μ复制子维持质粒的高拷贝。
 

载体序列

LOCUS       pYES2/NTA	6038 bp 	DNA     circular	SYN
DEFINITION  pYES2/NTA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="pYES2/NT A"
                     /mol_type="other DNA"
     promoter        1..451
                     /label="GAL1_promoter"
     misc_feature    414..437
                     /label="GAL1_primer"
     promoter        475..493
                     /label="T7_promoter"
     misc_feature    542..560
                     /label="Xpress_fwd_primer"
     misc_feature    543..575
                     /label="T7_leader"
     misc_feature    579..602
                     /label="Xpress_EK"
     terminator      791..1036
                     /label="CYC1_terminator"
     misc_feature    complement(800..818)
                     /label="CYC1_primer"
     misc_feature    complement(800..818)
                     /label="pYESTrp_rev_primer"
     rep_origin      complement(1264..1883)
                     /label="pBR322_origin"
     CDS             complement(2038..2898)
                     /label="ORF frame 3"
     CDS             complement(2994..3797)
                     /label="ORF frame 1"
     gene            complement(2997..3797)
                     /label="URA3"
                     /gene="URA3"
     CDS             3286..3762
                     /label="ORF frame 1"
     promoter        complement(3799..4024)
                     /label="URA3_promoter"
     rep_origin      4027..5498
                     /label="2micron_origin"
ORIGIN
    1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
   61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
  121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
  181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
  241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
  301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
  361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
  421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
  481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
  541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
  601 AGGTACCTAG GATCCAGTGT GGTGGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC
  661 GAGTCTAGAG GGCCCTTCGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT
  721 ACGCGTACCG GTCATCATCA CCATCACCAT TGAGTTTAAA CCCGCTGATC CTAGAGGGCC
  781 GCATCATGTA ATTAGTTATG TCACGCTTAC ATTCACGCCC TCCCCCCACA TCCGCTCTAA
  841 CCGAAAAGGA AGGAGTTAGA CAACCTGAAG TCTAGGTCCC TATTTATTTT TTTATAGTTA
  901 TGTTAGTATT AAGAACGTTA TTTATATTTC AAATTTTTCT TTTTTTTCTG TACAGACGCG
  961 TGTACGCATG TAACATTATA CTGAAAACCT TGCTTGAGAA GGTTTTGGGA CGCTCGAAGG
 1021 CTTTAATTTG CAAGCTGCGG CCCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG
 1081 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
 1141 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
 1201 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGCCCAGGA ACCGTAAAAA
 1261 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
 1321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
 1381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
 1441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
 1501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
 1561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
 1621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
 1681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
 1741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
 1801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
 1861 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
 1921 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
 1981 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
 2041 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
 2101 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG CGCTTACCAT CTGGCCCCAG
 2161 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
 2221 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
 2281 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
 2341 TGTTGGCATT GCTACAGGCA TCGTGGTGTC ACTCTCGTCG TTTGGTATGG CTTCATTCAG
 2401 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
 2461 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
 2521 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
 2581 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
 2641 TTGCCCGGCG TCAATACGGG ATAATAGTGT ATCACATAGC AGAACTTTAA AAGTGCTCAT
 2701 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
 2761 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
 2821 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
 2881 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATGGGTA ATAACTGATA TAATTAAATT
 2941 GAAGCTCTAA TTTGTGAGTT TAGTATACAT GCATTTACTT ATAATACAGT TTTTTAGTTT
 3001 TGCTGGCCGC ATCTTCTCAA ATATGCTTCC CAGCCTGCTT TTCTGTAACG TTCACCCTCT
 3061 ACCTTAGCAT CCCTTCCCTT TGCAAATAGT CCTCTTCCAA CAATAATAAT GTCAGATCCT
 3121 GTAGAGACCA CATCATCCAC GGTTCTATAC TGTTGACCCA ATGCGTCTCC CTTGTCATCT
 3181 AAACCCACAC CGGGTGTCAT AATCAACCAA TCGTAACCTT CATCTCTTCC ACCCATGTCT
 3241 CTTTGAGCAA TAAAGCCGAT AACAAAATCT TTGTCGCTCT TCGCAATGTC AACAGTACCC
 3301 TTAGTATATT CTCCAGTAGA TAGGGAGCCC TTGCATGACA ATTCTGCTAA CATCAAAAGG
 3361 CCTCTAGGTT CCTTTGTTAC TTCTTCTGCC GCCTGCTTCA AACCGCTAAC AATACCTGGG
 3421 CCCACCACAC CGTGTGCATT CGTAATGTCT GCCCATTCTG CTATTCTGTA TACACCCGCA
 3481 GAGTACTGCA ATTTGACTGT ATTACCAATG TCAGCAAATT TTCTGTCTTC GAAGAGTAAA
 3541 AAATTGTACT TGGCGGATAA TGCCTTTAGC GGCTTAACTG TGCCCTCCAT GGAAAAATCA
 3601 GTCAAGATAT CCACATGTGT TTTTAGTAAA CAAATTTTGG GACCTAATGC TTCAACTAAC
 3661 TCCAGTAATT CCTTGGTGGT ACGAACATCC AATGAAGCAC ACAAGTTTGT TTGCTTTTCG
 3721 TGCATGATAT TAAATAGCTT GGCAGCAACA GGACTAGGAT GAGTAGCAGC ACGTTCCTTA
 3781 TATGTAGCTT TCGACATGAT TTATCTTCGT TTCCTGCAGG TTTTTGTTCT GTGCAGTTGG
 3841 GTTAAGAATA CTGGGCAATT TCATGTTTCT TCAACACTAC ATATGCGTAT ATATACCAAT
 3901 CTAAGTCTGT GCTCCTTCCT TCGTTCTTCC TTCTGTTCGG AGATTACCGA ATCAAAAAAA
 3961 TTTCAAAGAA ACCGAAATCA AAAAAAAGAA TAAAAAAAAA ATGATGAATT GAATTGAAAA
 4021 GCTAGCTTAT CGATGATAAG CTGTCAAAGA TGAGAATTAA TTCCACGGAC TATAGACTAT
 4081 ACTAGATACT CCGTCTACTG TACGATACAC TTCCGCTCAG GTCCTTGTCC TTTAACGAGG
 4141 CCTTACCACT CTTTTGTTAC TCTATTGATC CAGCTCAGCA AAGGCAGTGT GATCTAAGAT
 4201 TCTATCTTCG CGATGTAGTA AAACTAGCTA GACCGAGAAA GAGACTAGAA ATGCAAAAGG
 4261 CACTTCTACA ATGGCTGCCA TCATTATTAT CCGATGTGAC GCTGCAGCTT CTCAATGATA
 4321 TTCGAATACG CTTTGAGGAG ATACAGCCTA ATATCCGACA AACTGTTTTA CAGATTTACG
 4381 ATCGTACTTG TTACCCATCA TTGAATTTTG AACATCCGAA CCTGGGAGTT TTCCCTGAAA
 4441 CAGATAGTAT ATTTGAACCT GTATAATAAT ATATAGTCTA GCGCTTTACG GAAGACAATG
 4501 TATGTATTTC GGTTCCTGGA GAAACTATTG CATCTATTGC ATAGGTAATC TTGCACGTCG
 4561 CATCCCCGGT TCATTTTCTG CGTTTCCATC TTGCACTTCA ATAGCATATC TTTGTTAACG
 4621 AAGCATCTGT GCTTCATTTT GTAGAACAAA AATGCAACGC GAGAGCGCTA ATTTTTCAAA
 4681 CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAAAGCG CTATTTTACC
 4741 AACGAAGAAT CTGTGCTTCA TTTTTGTAAA ACAAAAATGC AACGCGACGA GAGCGCTAAT
 4801 TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG AAATGCAACG CGAGAGCGCT
 4861 ATTTTACCAA CAAAGAATCT ATACTTCTTT TTTGTTCTAC AAAAATGCAT CCCGAGAGCG
 4921 CTATTTTTCT AACAAAGCAT CTTAGATTAC TTTTTTTCTC CTTTGTGCGC TCTATAATGC
 4981 AGTCTCTTGA TAACTTTTTG CACTGTAGGT CCGTTAAGGT TAGAAGAAGG CTACTTTGGT
 5041 GTCTATTTTC TCTTCCATAA AAAAAGCCTG ACTCCACTTC CCGCGTTTAC TGATTACTAG
 5101 CGAAGCTGCG GGTGCATTTT TTCAAGATAA AGGCATCCCC GATTATATTC TATACCGATG
 5161 TGGATTGCGC ATACTTTGTG AACAGAAAGT GATAGCGTTG ATGATTCTTC ATTGGTCAGA
 5221 AAATTATGAA CGGTTTCTTC TATTTTGTCT CTATATACTA CGTATAGGAA ATGTTTACAT
 5281 TTTCGTATTG TTTTCGATTC ACTCTATGAA TAGTTCTTAC TACAATTTTT TTGTCTAAAG
 5341 AGTAATACTA GAGATAAACA TAAAAAATGT AGAGGTCGAG TTTAGATGCA AGTTCAAGGA
 5401 GCGAAAGGTG GATGGGTAGG TTATATAGGG ATATAGCACA GAGATATATA GCAAAGAGAT
 5461 ACTTTTGAGC AATGTTTGTG GAAGCGGTAT TCGCAATGGG AAGCTCCACC CCGGTTGATA
 5521 ATCAGAAAAG CCCCAAAAAC AGGAAGATTG TATAAGCAAA TATTTAAATT GTAAACGTTA
 5581 ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACGAATAGC
 5641 CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG
 5701 TTCCAGTTTC CAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA
 5761 AAAGGGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG
 5821 GGTCGAGGTG CCGTAAAGCA GTAAATCGGA AGGGTAAACG GATGCCCCCA TTTAGAGCTT
 5881 GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGGG
 5941 CTAGGGCGGT GGGAAGTGTA GGGGTCACGC TGGGCGTAAC CACCACACCC GCCGCGCTTA
 6001 ATGGGGCGCT ACAGGGCGCG TGGGGATGAT CCACTAGT
//

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载体应用举例

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