| 质粒名称: | pCI-neo-cOVA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 25097 |
| 存储实验室: | Maria Castro |
|
载体骨架基本信息 |
|
| 载体名称: | pCI-neo |
| 载体出品公司: | Clontech |
| 空载体大小: | 5472bp |
| 完整质粒大小: | 6273bp |
| 载体类型: | 哺乳细胞表达、细菌表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | cytoplasmic ovalbumin / OVA |
| 物种来源: | G. gallus (chicken) |
| 插入大小: | 789 bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (destroyed during cloning) |
| 3’克隆位点: | EcoRI (destroyed during cloning) |
| 5’测序引物 | T7 EEV |
|
文献引用方法 |
|
| 材料和方法部分: |
pCI-neo-cOVA was a gift from Maria Castro (Addgene plasmid # 25097 ; http://n2t.net/addgene:25097 ; RRID:Addgene_25097)
|
| 参考文献部分: | Kupfer-type immunological synapse characteristics do not predict anti-brain tumor cytolytic T-cell function in vivo. Yang J, Sanderson NS, Wawrowsky K, Puntel M, Castro MG, Lowenstein PR. Proc Natl Acad Sci U S A. 2010 Mar 9. 107(10):4716-21. 10.1073/pnas.0911587107 PubMed 20133734 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCI-neo-cOVA 6273 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCI-neo-cOVA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6273) AUTHORS Yang J, Sanderson NS, Wawrowsky K, Puntel M, Castro MG, Lowenstein PR TITLE Kupfer-type immunological synapse characteristics do not predict anti-brain tumor cytolytic T-cell function in vivo. JOURNAL Proc Natl Acad Sci U S A. 2010 Mar 9. 107(10):4716-21. PUBMED 20133734 REFERENCE 2 (bases 1 to 6273) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..6273 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 79..458 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 459..670 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 621..641 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 667..691 /label=LNCX /note="Human CMV promoter, forward primer" intron 831..963 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 946..965 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 1008..1026 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1442..1465 /codon_start=1 /product="antigenic CD8+ T cell epitope from chicken ovalbumin" /label=OVA peptide /translation="SIINFEKL" promoter complement(1883..1900) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 1918..2039 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1929..1948) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1983..2002 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin 2225..2680 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2312..2331) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2522..2543 /label=F1ori-F /note="F1 origin, forward primer" promoter 2797..3154 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2820..2840) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3005..3140 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3067..3086 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3205..3999 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3259..3278) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3869..3888 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4063..4111 /note="synthetic polyadenylation signal" primer_bind complement(4152..4171) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4271..4293 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4331..4349) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4417..4521 /gene="bla" /label=AmpR promoter CDS 4522..5382 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4740..4759) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5553..6141 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6042..6061 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc 61 caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa tcaattacgg 121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 301 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg 361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt 421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 481 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 601 gcgatcgccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 661 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca 721 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag 781 tgactctctt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca 841 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac 901 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca 961 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac 1021 tataggctag cctcgagaat tcgtcgacat gggctccatc ggcgcagcaa gcatggaatt 1081 ttgttttgat gtattcaagg agctcatcaa ttcctgggta gaaagtcaga caaatggaat 1141 tatcagaaat gtccttcagc caagctccgt ggattctcaa actgcaatgg ttctggttaa 1201 tgccattgtc ttcaaaggac tgtgggagaa aacatttaag gatgaagaca cacaagcaat 1261 gcctttcaga gtgactgagc aagaaagcaa acctgtgcag atgatgtacc agattggttt 1321 atttagagtg gcatcaatgg cttctgagaa aatgaagatc ctggagcttc catttgccag 1381 tgggacaatg agcatgttgg tgctgttgcc tgatgaagtc tcaggccttg agcagcttga 1441 gagtataatc aactttgaaa aactgactga atggaccagt tctaatgtta tggaagagag 1501 gaagatcaaa gtgtacttac ctcgcatgaa gatggaggaa aaatacaacc tcacatctgt 1561 cttaatggct atgggcatta ctgacgtgtt tagctcttca gccaatctgt ctggcatctc 1621 ctcagcagag agcctgaaga tatctcaagc tgtccatgca gcacatgcag aaatcaatga 1681 agcaggcaga gaggtggtag ggtcagcaga ggctggagtg gatgctgcaa gcgtctctga 1741 agaatttagg gctgaccatc cattcctctt ctgtatcaag cacatcgcaa ccaacgccgt 1801 tctcttcttt ggcagatgtg tttcccctta agtcgacgaa ttcacgcgtg gtacctctag 1861 agtcgacccg ggcggccgct tccctttagt gagggttaat gcttcgagca gacatgataa 1921 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 1981 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 2041 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt 2101 aaagcaagta aaacctctac aaatgtggta aaatccgata aggatcgatc cgggctggcg 2161 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 2221 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 2281 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 2341 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 2401 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 2461 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 2521 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 2581 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 2641 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc 2701 ttacgcatct gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga 2761 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg 2821 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2881 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 2941 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 3001 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 3061 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 3121 aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt 3181 ctcgaactta aggctagagc caccatgatt gaacaagatg gattgcacgc aggttctccg 3241 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 3301 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 3361 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 3421 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 3481 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 3541 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 3601 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 3661 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 3721 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 3781 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 3841 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 3901 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 3961 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 4021 tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt 4081 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat ccgcgtatgg 4141 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 4201 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 4261 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 4321 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 4381 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 4441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 4501 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 4561 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 4621 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 4681 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 4741 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 4801 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 4861 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 4921 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 4981 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 5041 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 5101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 5161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 5221 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 5281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 5341 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 5401 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 5461 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 5521 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 5581 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 5641 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 5701 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 5761 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 5821 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 5881 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 5941 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 6001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 6061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 6121 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 6181 tgctggcctt ttgctcacat ggctcgacag atcttcaata ttggccatta gccatattat 6241 tcattggtta tatagcataa atcaatattg gct //
