| 质粒名称: | dClover2-N1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 54538 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | dClover2-N1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4750bp |
| 完整质粒大小: | 4732bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
| 启动子: | CMV |
| 标签/融合蛋白: | dClover2 (C terminal on backbone) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | dClover2-N1 was a gift from Michael Davidson (Addgene plasmid # 54538 ; http://n2t.net/addgene:54538 ; RRID:Addgene_54538) |
| 参考文献部分: | Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J. Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. 10.1038/srep20889 PubMed 26879144 |
买家导航
dClover2-N1载体质粒基本信息
dClover2-N1质粒图谱载体图谱和dClover2-N1载体序列质粒序列多克隆位点信息
dClover2-N1质粒载体简介
dClover2-N1质粒序列载体序列
LOCUS dClover2-N1 4732 bp DNA circular SYN 14-APR-2025
DEFINITION Localization: N1 Cloning Vector, Excitation: 505, Emission: 515.
This plasmid encodes dClover2 A206K, H231L based on publication..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4732)
AUTHORS Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW,
Lin MZ, Chu J
TITLE Improving brightness and photostability of green and red fluorescent
proteins for live cell imaging and FRET reporting.
JOURNAL Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
PUBMED 26879144
REFERENCE 2 (bases 1 to 4732)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep.";
date: "2016-02-16"; volume: "6:20889. doi"; pages: "
10.1038/srep20889"
COMMENT Sequence Label: dClover2-N1
FEATURES Location/Qualifiers
source 1..4732
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 598..678
/label=MCS
/note="multiple cloning site"
regulatory 680..689
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 686..1405
/codon_start=1
/product="monomeric derivative of the bright green-yellow
fluorescent protein Clover"
/label=mClover2
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(731..752)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind 1339..1360
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1527..1648
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1564..1583)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1618..1637
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1655..2110)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1792..1813)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2004..2023
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2137..2241
/gene="bla"
/label=AmpR promoter
promoter 2243..2600
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2243..2434
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2266..2286)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2451..2586
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2635..3429
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2635..3429
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2689..2708)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3299..3318
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3617..3636)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3661..3708
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4037..4625
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4526..4545
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
661 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
721 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtccgcgg
781 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg
841 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tggcctgctt
901 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
961 ctacgtccag gagcgcacca tctctttcaa ggacgacggt acctacaaga cccgcgccga
1021 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
1081 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc actacgtcta
1141 tatcacggcc gacaagcaga agaacagcat caaggctaac ttcaagatcc gccacaacgt
1201 tgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
1261 ccccgtgctg ctgcccgaca accactacct gagccatcag tccaagctga gcaaagaccc
1321 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
1381 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
1441 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
1501 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
1561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
1621 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
1681 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
1741 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
1801 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
1861 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
1921 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
1981 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
2041 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
2101 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
2161 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
2221 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
2281 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
2341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
2401 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
2461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
2521 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
2581 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
2641 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
2701 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
2761 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
2821 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
2881 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
2941 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
3001 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
3061 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
3121 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
3181 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
3241 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
3301 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
3361 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
3421 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
3481 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
3541 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
3601 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
3661 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
3721 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
3781 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
3841 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
3901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
3961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
4021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
4081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
4141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
4201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
4261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
4321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
4381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
4441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
4501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
4561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
4621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
4681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gc
//
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