| 质粒名称: | pCAP01 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 59981 |
| 存储实验室: | Bradley Moore |
|
载体骨架基本信息 |
|
| 载体名称: | SuperCos1 |
| 载体出品公司: | Agilent |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 7600 bp |
| 载体修饰: | 将RP4起始点和attP位点插入到异源表达放线菌生物合成基因簇的载体中。该载体还含有TRP1基因和CEN6\u-ARS4，用于在S。酿酒酵母色氨酸缺乏突变体。 |
| 载体类型: | 细菌表达、酵母表达、合成生物学；转化相关重组克隆 |
| 筛选标记: | TRP1, URA3 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 克隆方法: | Ligation Independent克隆 |
| 5' 测序引物 | TATGTCCTGCGGGTAAATAG |
| 3' 测序引物 | TCGGGGAAATGTGCGCGGAA |
|
文献引用方法 |
|
| 材料和方法部分: | pCAP01 was a gift from Bradley Moore (Addgene plasmid # 59981 ; http://n2t.net/addgene:59981 ; RRID:Addgene_59981) |
| 参考文献部分: | Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Yamanaka K, Reynolds KA, Kersten RD, Ryan KS, Gonzalez DJ, Nizet V, Dorrestein PC, Moore BS. Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1957-62. doi: 10.1073/pnas.1319584111. Epub 2014 Jan 21. 10.1073/pnas.1319584111 PubMed 24449899 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAP01 9022 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION The function of this material is to facilitate the capture and
expression of bacterial gene clusters..
ACCESSION .
VERSION .
KEYWORDS pCAP01
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9022)
AUTHORS Yamanaka K, Reynolds KA, Kersten RD, Ryan KS, Gonzalez DJ, Nizet V,
Dorrestein PC, Moore BS
TITLE Direct cloning and refactoring of a silent lipopeptide biosynthetic
gene cluster yields the antibiotic taromycin A.
JOURNAL Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1957-62. doi:
10.1073/pnas.1319584111. Epub 2014 Jan 21.
PUBMED 24449899
REFERENCE 2 (bases 1 to 9022)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9022
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 84..587
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
primer_bind 629..647
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(685..707)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 807..826
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 847..1126
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
CDS 1127..1801
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
primer_bind complement(2041..2062)
/label=F1ori-F
/note="F1 origin, forward primer"
rep_origin 2309..2897
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2798..2817
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3062..3079
/label=L4440
/note="L4440 vector, forward primer"
polyA_signal 3293..3427
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3317..3336)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3371..3390
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(3852..3872)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 4003..4068
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS complement(4488..5282)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4599..4618)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5209..5228
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
CDS complement(6066..6437)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 6470..6579
/note="incP origin of transfer"
CDS complement(6817..6828)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 6899..6998
/label=phage phi-C31 attP
/bound_moiety="phage phi-C31 integrase"
/note="attachment site of phage phi-C31"
CDS 7008..8825
/codon_start=1
/product="integrase from phage phi-C31"
/label=phage phi-C31 integrase
/translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
1 acttgaggta cctgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
61 aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa taattataat ttaaattttt
121 taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt
181 tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc
241 ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac
301 cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt
361 taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt
421 taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt
481 tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt
541 ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt cctaagaaac
601 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
661 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
721 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
781 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
841 tagatcacga cattactata tatataatat aggaagcatt taatagacag catcgtaata
901 tatgtgtact ttgcagttat gacgccagat ggcagtagtg gaagatattc tttattgaaa
961 aatagcttgt caccttacgt acaatcttga tccggagctt ttcttttttt gccgattaag
1021 aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt
1081 gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa
1141 tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc
1201 agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa
1261 gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa
1321 tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt
1381 tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg
1441 gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga
1501 ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga
1561 ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg
1621 aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa
1681 tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga
1741 gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata
1801 ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgccgat ctatgcggtg
1861 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
1921 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
1981 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
2041 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
2101 accgtctatc agggcgatgg cccactacgt gaacctaact gtcagaccaa gtttactcat
2161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
2221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
2281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
2341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
2401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
2461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
2521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cctaccgggt
2581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
2641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
2701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
2761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
2821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
2881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
2941 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
3001 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
3061 cagcgagtca gtgagcgagg aagcggaaga gcgctgactt ccgcgtttcc agactttacg
3121 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
3181 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
3241 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt cagatccaga
3301 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
3361 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
3421 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
3481 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc
3541 aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa gctaaaggta
3601 cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg agtaagaaaa
3661 aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga atatttttcc
3721 ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca
3781 agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc
3841 ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta
3901 gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca
3961 ttccaccact gctcccattc atcagtttcc ataggttgga atctaaaata cacaaacaat
4021 tagaatcagt agtttaacac attatacact taaaaatttt atatttacct tagagcttta
4081 aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa
4141 gatccggacc aaagcggcca tcgtgcctcc ccactcctgc agttcggggg catggatgcg
4201 cggatagccg ctgctggttt cctggatgcc gacggatttg cactgccggt agaactccgc
4261 gaggtcgtcc agcctcaggc agcagctgaa ccaactcgcg aggggatcga gcccggggtg
4321 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
4381 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
4441 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
4501 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
4561 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
4621 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
4681 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
4741 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
4801 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
4861 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
4921 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
4981 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
5041 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
5101 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
5161 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
5221 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
5281 atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc
5341 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
5401 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
5461 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
5521 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
5581 gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgagagctca
5641 tgagcggaga acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg
5701 tggagcggat cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc
5761 gtttggcctc cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg
5821 acggcgaatg gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact
5881 caccgcgacg tatcgggccc tggccagcta gctagagtcg acctgcaggt ccccggggat
5941 cggtcttgcc ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga
6001 tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa
6061 aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc
6121 tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
6181 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
6241 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
6301 ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc
6361 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
6421 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
6481 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
6541 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
6601 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
6661 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt
6721 cgacctgcag gcatgcaagc tctagcgatt ccagacgtcc cgaaggcgtg gcgcggcttc
6781 cccgtgccgg agcaatcgcc ctgggtgggt tacacgacgc ccctctatgg cccgtactga
6841 cggacacacc gaagccccgg cggcaaccct cagcggatgc cccggggctt cacgttttcc
6901 caggtcagaa gcggttttcg ggagtagtgc cccaactggg gtaacctttg agttctctca
6961 gttgggggcg tagggtcgcc gacatgacac aaggggttgt gaccggggtg gacacgtacg
7021 cgggtgctta cgaccgtcag tcgcgcgagc gcgaaaattc gagcgcagca agcccagcga
7081 cacagcgtag cgccaacgaa gacaaggcgg ccgaccttca gcgcgaagtc gagcgcgacg
7141 ggggccggtt caggttcgtc gggcatttca gcgaagcgcc gggcacgtcg gcgttcggga
7201 cggcggagcg cccggagttc gaacgcatcc tgaacgaatg ccgcgccggg cggctcaaca
7261 tgatcattgt ctatgacgtg tcgcgcttct cgcgcctgaa ggtcatggac gcgattccga
7321 ttgtctcgga attgctcgcc ctgggcgtga cgattgtttc cactcaggaa ggcgtcttcc
7381 ggcagggaaa cgtcatggac ctgattcacc tgattatgcg gctcgacgcg tcgcacaaag
7441 aatcttcgct gaagtcggcg aagattctcg acacgaagaa ccttcagcgc gaattgggcg
7501 ggtacgtcgg cgggaaggcg ccttacggct tcgagcttgt ttcggagacg aaggagatca
7561 cgcgcaacgg ccgaatggtc aatgtcgtca tcaacaagct tgcgcactcg accactcccc
7621 ttaccggacc cttcgagttc gagcccgacg taatccggtg gtggtggcgt gagatcaaga
7681 cgcacaaaca ccttcccttc aagccgggca gtcaagccgc cattcacccg ggcagcatca
7741 cggggctttg taagcgcatg gacgctgacg ccgtgccgac ccggggcgag acgattggga
7801 agaagaccgc ttcaagcgcc tgggacccgg caaccgttat gcgaatcctt cgggccccgc
7861 gtattgcggg cttcgccgct gaggtgatct acaagaagaa gccggacggc acgccgacca
7921 cgaagattga gggttaccgc attcagcgcg acccgatcac gctccggccg gtcgagcttg
7981 attgcggacc gatcatcgag cccgctgagt ggtatgagct tcaggcgtgg ttggacggca
8041 gggggcgcgg caaggggctt tcccgggggc aagccattct gtccgccatg gacaagctgt
8101 actgcgagtg tggcgccgtc atgacttcga agcgcgggga agaatcgatc aaggactctt
8161 accgctgccg tcgccggaag gtggtcgacc cgtccgcacc tgggcagcac gaaggcacgt
8221 gcaacgtcag catggcggca ctcgacaagt tcgttgcgga acgcatcttc aacaagatca
8281 ggcacgccga aggcgacgaa gagacgttgg cgcttctgtg ggaagccgcc cgacgcttcg
8341 gcaagctcac tgaggcgcct gagaagagcg gcgaacgggc gaaccttgtt gcggagcgcg
8401 ccgacgccct gaacgccctt gaagagctgt acgaagaccg cgcggcaggc gcgtacgacg
8461 gacccgttgg caggaagcac ttccggaagc aacaggcagc gctgacgctc cggcagcaag
8521 gggcggaaga gcggcttgcc gaacttgaag ccgccgaagc cccgaagctt ccccttgacc
8581 aatggttccc cgaagacgcc gacgctgacc cgaccggccc taagtcgtgg tgggggcgcg
8641 cgtcagtaga cgacaagcgc gtgttcgtcg ggctcttcgt agacaagatc gttgtcacga
8701 agtcgactac gggcaggggg cagggaacgc ccatcgagaa gcgcgcttcg atcacgtggg
8761 cgaagccgcc gaccgacgac gacgaagacg acgcccagga cggcacggaa gacgtagcgg
8821 cgtagcgaga cacccgggaa gcctgatcta cgtctgtcga gaagtttctg atcgaaaagt
8881 tcgacagcgt ctccgacctg atgcagctct cgcagggcga agaatctcgt gctttcagct
8941 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
9001 aagatcgact agtaacctcg ag
//
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