pGL4.80[hRlucP/Neo]

价格:1500元

联系方式:I47-825O-882O

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pGL4.80载体基本信息

出品公司: Promega
载体名称: pGL4.80 ; pGL4.80[hRlucP/Neo]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 5010 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRlucP
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.80[hRlucP/Neo]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRlucP。
产品目录号: E6981
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.80载体质粒图谱和多克隆位点信息

pGL4.80载体图谱



pGL4.80 多克隆位点

pGL4.80 载体特征

pGL4.80载体简介

pGL4.80载体简介
The pGL4.80[hRlucP/Neo] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.80[hRlucP/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucP reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with a greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

pGL4.80载体序列

LOCUS       DQ188844                5010 bp    DNA     circular SYN 16-NOV-2005
DEFINITION  Synthetic Renilla luciferase reporter vector pGL4.80[hRlucP/Neo],
            complete sequence.
ACCESSION   DQ188844
VERSION     DQ188844.1  GI:76364306
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.80[hRlucP/Neo]
  ORGANISM  Renilla luciferase reporter vector pGL4.80[hRlucP/Neo]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Renilla luciferase reporter vector
                     pGL4.80[hRlucP/Neo]"
                     /mol_type="other DNA"
                     /db_xref="taxon:346091"
     misc_feature    1..70
                     /note="multiple cloning region"
     CDS             100..1158
                     /note="synthetic Renilla luciferase; contains hPEST
                     destabilization sequence"
                     /codon_start=1
                     /product="hRlucP"
                     /protein_id="ABA41674.1"
                     /db_xref="GI:76364307"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
                     ESGMDRHPAACASARINV"
     regulatory      1198..1419
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
     regulatory      1467..1885
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter"
     CDS             1910..2704
                     /note="neomycin resistance"
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /protein_id="ABA41675.1"
                     /db_xref="GI:76364308"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      2729..2777
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal"
     primer_bind     2844..2863
                     /note="Reporter Vector primer 4 (RVprimer4) binding
                     region"
     rep_origin      3101
                     /note="ColEI-derived plasmid replication origin"
     gene            complement(3892..4752)
                     /gene="bla"
     CDS             complement(3892..4752)
                     /gene="bla"
                     /note="ampicillin resistance"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="ABA41676.1"
                     /db_xref="GI:76364309"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4857..5010
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal/transcriptional pause
                     site"
     primer_bind     4959..4978
                     /note="Reporter Vector primer 3 (RVprimer3) binding
                     region"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
     1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
     1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
     1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
     1441 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
     1501 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     1561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     1621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     1741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     1861 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgatcgaaca
     1921 agacggcctc catgctggca gtcccgcagc ttgggtcgaa cgcttgttcg ggtacgactg
     1981 ggcccagcag accatcggat gtagcgatgc ggccgtgttc cgtctaagcg ctcaaggccg
     2041 gcccgtgctg ttcgtgaaga ccgacctgag cggcgccctg aacgagcttc aagacgaggc
     2101 tgcccgcctg agctggctgg ccaccaccgg cgtaccctgc gccgctgtgt tggatgttgt
     2161 gaccgaagcc ggccgggact ggctgctgct gggcgaggtc cctggccagg atctgctgag
     2221 cagccacctt gcccccgctg agaaggtttc tatcatggcc gatgcaatgc ggcgcctgca
     2281 caccctggac cccgctacct gccccttcga ccaccaggct aagcatcgga tcgagcgtgc
     2341 tcggacccgc atggaggccg gcctggtgga ccaggacgac ctggacgagg agcatcaggg
     2401 cctggccccc gctgaactgt tcgcccgact gaaagcccgc atgccggacg gtgaggacct
     2461 ggttgtcaca cacggagatg cctgcctccc taacatcatg gtcgagaatg gccgcttctc
     2521 cggcttcatc gactgcggtc gcctaggagt tgccgaccgc taccaggaca tcgccctggc
     2581 cacccgcgac atcgctgagg agcttggcgg cgagtgggcc gaccgcttct tagtcttgta
     2641 cggcatcgca gctcccgaca gccagcgcat cgccttctac cgcttgctcg acgagttctt
     2701 ttaatgatct agaaccggtc atggccgcaa taaaatatct ttattttcat tacatctgtg
     2761 tgttggtttt ttgtgtgttc gaactagatg ctgtcgaccg atgcccttga gagccttcaa
     2821 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     2881 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
     2941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3841 atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg
     3901 cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg
     3961 actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc
     4021 aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc
     4081 agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag
     4141 ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc
     4201 cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg
     4261 ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc
     4321 cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat
     4381 ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg
     4441 cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc
     4501 ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg
     4561 gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat
     4621 atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg
     4681 gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg
     4741 ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta
     4801 cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata
     4861 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
     4921 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
     4981 gtgcaagtgc aggtgccaga acatttctct
//

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