| 质粒名称: | pLV-egfp-U3-Osp.gRNA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 166034 |
| 存储实验室: | Yujia Cai |
|
载体骨架基本信息 |
|
| 载体名称: | pCCL-PGK-egfp |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒，CRISPR |
| 筛选标记: | EGFP |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLV-egfp-U3-Osp.gRNA was a gift from Yujia Cai (Addgene plasmid # 166034 ; http://n2t.net/addgene:166034 ; RRID:Addgene_166034) |
| 参考文献部分: | Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice. Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J, Sun X, Yang H, Paludan SR, Cai Y. Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi: 10.1038/s41551-020-00656-y. 10.1038/s41551-020-00656-y PubMed 33398131 |
买家导航
pLV-egfp-U3-Osp.gRNA载体质粒基本信息
pLV-egfp-U3-Osp.gRNA质粒图谱载体图谱和pLV-egfp-U3-Osp.gRNA载体序列质粒序列多克隆位点信息
pLV-egfp-U3-Osp.gRNA质粒载体简介
pLV-egfp-U3-Osp.gRNA质粒序列载体序列
LOCUS pLV-egfp-U3-Osp.gRNA 8119 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Empty backbone for expression of gRNA-expressing integration
deficient lentiviral particles..
ACCESSION .
VERSION .
KEYWORDS pLV-egfp-U3-Osp.gRNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8119)
AUTHORS Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J,
Sun X, Yang H, Paludan SR, Cai Y
TITLE Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting
guide RNA prevents wet age-related macular degeneration in mice.
JOURNAL Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi:
10.1038/s41551-020-00656-y.
PUBMED 33398131
REFERENCE 2 (bases 1 to 8119)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8119
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 219..399
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 446..571
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1063..1296
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1481..1525
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1792..1909
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 1948..2447
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(1983..2006)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2380..2399
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 2471..2480
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2477..3196
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2477..3193
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2522..2543)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2783..2802)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3130..3151
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 3220..3808
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3273..3293)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3691..3702)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 3929..4169
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 3929..3949
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 4100..4119
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
LTR 4333..4513
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(4542..4560)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4543..4560)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4806..4825)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4807..4825)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4835..4852)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4835..4851)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4844..4866)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 4993..5448
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5080..5099)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5290..5311
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 5474..5578
/gene="bla"
/label=AmpR promoter
CDS 5579..6439
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5797..5816)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6610..7198
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7099..7118
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7352..7369
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7486..7507
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7522..7552
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7560..7576
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7565..7587
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7584..7600
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7584..7600
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 7619..7639
/label=T3
/note="T3 promoter, forward primer"
promoter 7621..7639
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
enhancer 7740..8119
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg ttagaccaga
241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
421 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
481 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
541 ctagaaggag agagatgggt gcgagagcgt cagtattaag cggggagaat tagatcgcga
601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1741 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
1801 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
1861 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga
1921 gactagcctc gacgatggtc gagtaccggg taggggaggc gcttttccca aggcagtctg
1981 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
2041 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
2101 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
2161 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
2221 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
2281 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
2341 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
2401 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tctagcggga
2461 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
2521 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
2581 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
2641 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
2701 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
2761 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
2821 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
2881 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
2941 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
3001 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
3061 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
3121 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
3181 cgagctgtac aagtaaagcg gcctcgaggg aattccgata atcaacctct ggattacaaa
3241 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
3301 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
3361 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
3421 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
3481 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
3541 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
3601 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
3661 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
3721 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
3781 cggatctccc tttgggccgc ctccccgcat cgggaattcg agctcggtac ctttaagacc
3841 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
3901 agggctaatt cactcccaac gaagacaaga gggcctattt cccatgattc cttcatattt
3961 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
4021 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
4081 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
4141 cttggcttta tatatcttgt ggaaaggacg aaacaccaaa agcaggtgga catacaagtc
4201 gacaatagca cctgcaaaag tttcagagct atgctggaaa cagcatagca agttgaaata
4261 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgatctgctt
4321 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
4381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
4441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
4501 aaaatctcta gcagcatcta gctagaatta attccgtgta ttctatagtg tcacctaaat
4561 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
4621 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
4681 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
4741 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagcctagg gacgtaccca
4801 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
4861 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
4921 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
4981 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
5041 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
5101 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
5161 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
5221 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
5281 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
5341 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
5401 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
5461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
5521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
5581 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
5641 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
5701 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5761 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
5821 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
5881 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
5941 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
6001 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
6061 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
6121 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
6181 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6241 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6301 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
6361 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
6421 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
6481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
6541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
6601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
6961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7201 cgccagcaac gcggtctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
7261 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
7321 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
7381 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
7441 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
7501 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
7561 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
7621 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttggccat tgcatacgtt
7681 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
7741 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
7801 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
7861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
7921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
7981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
8041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
8101 agtcatcgct attaccatg
//
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