pCEP4

价格:1100元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pCEP4
质粒类型: 哺乳动物细胞表达载体
启动子: CMV
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 10410 bp
5' 测序引物:   CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物: EBV-R:5'-GTGGTTTGTCCAAACTCATC-3'
载体标签:
载体抗性: 氨苄青霉素
筛选标记: 潮霉素
备注:
pREP7载体是附加体型载体,可以在基因组外自主复制,类似的载体有:
   pREP4
   PCEP4
   pREP7
   pREP10
   pEBNA-DEST
   pEBVHisA
   pEBVHisB
   pEBVHisC
   pREP4/CAT
   pEBVHis-lacZ
 
产品目录号: V044-50
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCEP4载体图谱

pCEP4载体多克隆位点

载体简介

pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.

载体序列

LOCUS       pCEP4    10410 bp     DNA  circular    SYN
DEFINITION  pCEP4
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="pCEP4"
                     /mol_type="other DNA"
     misc_feature    112..134
                     /label="pGEX_3_primer"
     terminator      279..410
                     /label="SV40_PA_terminator"
     misc_feature    367..386
                     /label="EBV_rev_primer"
     promoter        complement(474..555)
                     /label="CMV_promoter"
     misc_feature    complement(474..498)
                     /label="LNCX_primer"
     misc_feature    complement(481..500)
                     /label="pCEP_fwd_primer"
     misc_feature    complement(524..544)
                     /label="CMV_fwd_primer"
     CDS             1305..2045
                     /label="ORF frame 3"
     terminator      complement(1566..1836)
                     /label="TK_PA_terminator"
     CDS             complement(1844..2869)
                     /label="ORF frame 3"
     gene            complement(1847..2854)
                     /label="hygroB"
                     /gene="hygroB"                  
     CDS             2111..3481
                     /label="ORF frame 2"                   
     promoter        2988..3136
                     /label="TATA_promoter"
     gene            3143..3481
                     /label="tet (852 - 1190)"
                     /gene="tet (852 - 1190)"
     CDS             3385..3996
                     /label="ORF frame 1"
     gene            complement(4430..5290)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4430..5290)
                     /label="ORF frame 3"
     promoter        complement(5332..5360)
                     /label="AmpR_promoter"
     CDS             complement(5914..7128)
                     /label="ORF frame 1"
     CDS             5916..7841
                     /label="ORF frame 3"
     CDS             6031..7143
                     /label="ORF frame 1"
     rep_origin      8145..10315
                     /label="EBV_origin"
ORIGIN
    1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
   61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
  121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
  181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
  241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
  301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
  361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
  421 ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttct agagatctga
  481 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
  541 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
  601 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
  661 tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
  721 acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag
  781 gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga
  841 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
  901 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
  961 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
 1021 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
 1081 catggcggtc atattggaca tgagccaata taaatgtaca tattatgata tagatacaac
 1141 gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct
 1201 aattgccaat attgattcaa tgtatagatc ttccatacct accagttctg cgcctgcagc
 1261 aatgcaacaa cgttgcccgg atctgcgatg ataagctgtc aaacatgaga attggtcgac
 1321 tagcttggca cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg tttggcagcc
 1381 acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg ccatccaaaa
 1441 accatgggtc tgtctgctca gtccagtcgt ggaccagacc ccacgcaacg cccaaaataa
 1501 taacccccac gaaccataaa ccattcccca tgggggaccc cgtccctaac ccacggggcc
 1561 agtggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
 1621 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
 1681 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
 1741 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
 1801 gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccctattcc tttgccctcg
 1861 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
 1921 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
 1981 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
 2041 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
 2101 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
 2161 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
 2221 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
 2281 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
 2341 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
 2401 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
 2461 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
 2521 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
 2581 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
 2641 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
 2701 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
 2761 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
 2821 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
 2881 cgggatctgc ggcacgctgt tgacgctgtt aagcgggtcg ctgcagggtc gctcggtgtt
 2941 cgaggccaca cgcgtcacct taatatgcga agtggacctg ggaccgcgcc gccccgactg
 3001 catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga
 3061 actaaagaca tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc
 3121 cacggggatg aagcagggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
 3181 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
 3241 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
 3301 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
 3361 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
 3421 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
 3481 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
 3541 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
 3601 gccatctcca gcagccgcac gcggcgcagc aaaaggccag gaaccgtaaa aaggccgcgt
 3661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
 3721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
 3781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
 3841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
 3901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
 3961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
 4021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
 4081 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
 4141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
 4201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
 4261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
 4321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
 4381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
 4441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
 4501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
 4561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
 4621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
 4681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
 4741 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
 4801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
 4861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
 4921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
 4981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
 5041 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
 5101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
 5161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
 5221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
 5281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
 5341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
 5401 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
 5461 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc
 5521 atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc cagtagcatc
 5581 tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat ccctaccgtc
 5641 cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg caaatcttga
 5701 ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg aggatggttc
 5761 ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag gggatgaggg
 5821 tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga gcgtcgtcta
 5881 ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg ccaggtacag
 5941 gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc tccggcggca
 6001 gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga ggacgaggac
 6061 gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga catagagatg
 6121 gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc cacggtggaa
 6181 caggagcagg agcaggagcg ggaggggcag gagcaggagg ggcaggagca ggaggagggg
 6241 caggagcagg aggaggggca ggaggggcag gaggggcagg aggggcagga gcaggaggag
 6301 gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag
 6361 gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
 6421 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag
 6481 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag
 6541 gggcaggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga ggggcaggag
 6601 gggcaggagc aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag
 6661 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg
 6721 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
 6781 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
 6841 gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga ggtggaggcc
 6901 ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg ggtcgaggag
 6961 gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt cgtgaaagag
 7021 ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt agtcagtcat
 7081 catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt ttccaccctg
 7141 taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt gagcctgacg
 7201 tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc ccaagcactg
 7261 gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt ggaaagcatc
 7321 gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta agagctctcc
 7381 tggctaggag tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc ggtgtgttcg
 7441 tatatggagg tagtaagacc tccctttaca acctaaggcg aggaactgcc cttgctattc
 7501 cacaatgtcg tcttacacca ttgagtcgtc tcccctttgg aatggcccct ggacccggcc
 7561 cacaacctgg cccgctaagg gagtccattg tctgttattt catggtcttt ttacaaactc
 7621 atatatttgc tgaggttttg aaggatgcga ttaaggacct tgttatgaca aagcccgctc
 7681 ctacctgcaa tatcagggtg actgtgtgca gctttgacga tggagtagat ttgcctccct
 7741 ggtttccacc tatggtggaa ggggctgccg cggagggtga tgacggagat gacggagatg
 7801 aaggaggtga tggagatgag ggtgaggaag ggcaggagtg atgtaacttg ttaggagacg
 7861 ccctcaatcg tattaaaagc cgtgtattcc cccgcactaa agaataaatc cccagtagac
 7921 atcatgcgtg ctgttggtgt atttctggcc atctgtcttg tcaccatttt cgtcctccca
 7981 acatggggca attgggcata cccatgttgt cacgtcactc agctccgcgc tcaacacctt
 8041 ctcgcgttgg aaaacattag cgacatttac ctggtgagca atcagacatg cgacggcttt
 8101 agcctggcct ccttaaattc acctaagaat gggagcaacc agcatgcagg aaaaggacaa
 8161 gcagcgaaaa ttcacgcccc cttgggaggt ggcggcatat gcaaaggata gcactcccac
 8221 tctactactg ggtatcatat gctgactgta tatgcatgag gatagcatat gctacccgga
 8281 tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
 8341 tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga
 8401 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
 8461 tatagattag gatagcatat gctacccaga tatagattag gatagcatat gctatccaga
 8521 tatttgggta gtatatgcta cccagatata aattaggata gcatatacta ccctaatctc
 8581 tattaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
 8641 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
 8701 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
 8761 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta tccagatatt
 8821 tgggtagtat atgctaccca tggcaacatt agcccaccgt gctctcagcg acctcgtgaa
 8881 tatgaggacc aacaaccctg tgcttggcgc tcaggcgcaa gtgtgtgtaa tttgtcctcc
 8941 agatcgcagc aatcgcgccc ctatcttggc ccgcccacct acttatgcag gtattccccg
 9001 gggtgccatt agtggttttg tgggcaagtg gtttgaccgc agtggttagc ggggttacaa
 9061 tcagccaagt tattacaccc ttattttaca gtccaaaacc gcagggcggc gtgtgggggc
 9121 tgacgcgtgc ccccactcca caatttcaaa aaaaagagtg gccacttgtc tttgtttatg
 9181 ggccccattg gcgtggagcc ccgtttaatt ttcgggggtg ttagagacaa ccagtggagt
 9241 ccgctgctgt cggcgtccac tctctttccc cttgttacaa atagagtgta acaacatggt
 9301 tcacctgtct tggtccctgc ctgggacaca tcttaataac cccagtatca tattgcacta
 9361 ggattatgtg ttgcccatag ccataaattc gtgtgagatg gacatccagt ctttacggct
 9421 tgtccccacc ccatggattt ctattgttaa agatattcag aatgtttcat tcctacacta
 9481 gtatttattg cccaaggggt ttgtgagggt tatattggtg tcatagcaca atgccaccac
 9541 tgaacccccc gtccaaattt tattctgggg gcgtcacctg aaaccttgtt ttcgagcacc
 9601 tcacatacac cttactgttc acaactcagc agttattcta ttagctaaac gaaggagaat
 9661 gaagaagcag gcgaagattc aggagagttc actgcccgct ccttgatctt cagccactgc
 9721 ccttgtgact aaaatggttc actaccctcg tggaatcctg accccatgta aataaaaccg
 9781 tgacagctca tggggtggga gatatcgctg ttccttagga cccttttact aaccctaatt
 9841 cgatagcata tgcttcccgt tgggtaacat atgctattga attagggtta gtctggatag
 9901 tatatactac tacccgggaa gcatatgcta cccgtttagg gttaacaagg gggccttata
 9961 aacactattg ctaatgccct cttgagggtc cgcttatcgg tagctacaca ggcccctctg
10021 attgacgttg gtgtagcctc ccgtagtctt cctgggcccc tgggaggtac atgtccccca
10081 gcattggtgt aagagcttca gccaagagtt acacataaag gcaatgttgt gttgcagtcc
10141 acagcatgca aagtctgctc caggatgaaa gccactcagt gttggcaaat gtgcacatcc
10201 atttataagg atgtcaacta cagtcagaga acccctttgt gtttggtccc cccccgtgtc
10261 acatgtggaa cagggcccag ttggcaagtt gtaccaacca actgaaggga ttacatgcac
10321 tgccccgaat acaaaacaaa agcgctcctc gtaccagcga agaaggggca gagatgccgt
10381 agtcaggttt agttcgtccg gcggcggggc
//

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