| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pCEP4 |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 10186 bp |
| 5' 测序引物: | CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3' |
| 3' 测序引物: | EBV-R:5'-GTGGTTTGTCCAAACTCATC-3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素 |
| 备注: |
pREP7载体是附加体型载体，可以在基因组外自主复制，类似的载体有： pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
| 产品目录号: | V044-50 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
载体序列
LOCUS pCEP4 10186 bp ds-DNA circular SYN 19-OCT-2017 DEFINITION Episomal mammalian vector with a CMV promoter and a hygromycin resistance marker, for high-level expression in primate and canine cells. ACCESSION . VERSION . KEYWORDS pCEP4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10186) AUTHORS Thermo Fisher (Invitrogen) TITLE Direct Submission JOURNAL Exported Thursday, July 9, 2020 from SnapGene Viewer 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10186 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 4..383 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 384..587 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 620..676 /note="MCS" /note="multiple cloning site" polyA_signal 672..806 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 1529..3318 /note="oriP" /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(3620..5545) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /note="EBNA1" /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" promoter 6066..6170 /gene="bla" /note="AmpR promoter" CDS 6171..7031 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7202..7790 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 8221..8366 /note="HSV TK promoter" /note="herpes simplex virus thymidine kinase promoter" CDS 8409..9446 /codon_start=1 /product="hygromycin B phosphotransferase" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE MGEAN" polyA_signal 9488..9536 /note="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" ORIGIN 1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat 421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc 541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc 601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgaggc 661 cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa accacaacta 721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa 781 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg 841 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg 901 ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 1021 tcaggcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga 1081 tcgatgcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg 1141 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac 1201 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat 1261 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt 1321 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca 1381 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt 1441 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag 1501 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta 1561 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct 1621 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct 1681 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac 1741 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt 1801 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat 1861 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg 1921 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa 1981 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg 2041 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata 2101 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag 2161 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca 2221 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg 2281 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc 2341 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg 2401 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc 2461 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg 2521 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct 2581 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta 2641 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct 2701 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct 2761 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct 2821 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct 2881 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca 2941 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc 3001 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc 3061 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc 3121 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc 3181 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag 3241 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg 3301 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct 3361 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag 3421 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt 3481 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat 3541 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg 3601 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt 3661 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc 3721 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag 3781 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat 3841 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg 3901 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg 3961 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata 4021 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca 4081 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac 4141 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc 4201 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca 4261 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta 4321 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg 4381 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg 4441 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac 4501 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc 4561 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc 4621 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg 4681 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg 4741 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc 4801 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc 4861 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc 4921 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc 4981 ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg 5041 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc 5101 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc 5161 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg 5221 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg 5281 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac 5341 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc 5401 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac 5461 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc 5521 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat 5581 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca 5641 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc 5701 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc 5761 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg 5821 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga 5881 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat 5941 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt 6001 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 6061 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 6121 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 6181 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 6241 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 6301 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 6361 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg 6421 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 6481 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 6541 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 6601 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 6661 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa 6721 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 6781 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 6841 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 6901 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 6961 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 7021 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 7081 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 7141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 7201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 7261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 7321 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 7381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 7441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 7501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 7561 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 7621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 7681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 7741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 7801 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt 7861 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga 7921 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc 7981 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga 8041 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc 8101 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt 8161 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg 8221 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg 8281 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc 8341 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc 8401 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa 8461 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca 8521 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct 8581 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc 8641 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg 8701 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg 8761 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac 8821 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc 8881 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc 8941 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg 9001 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga 9061 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt 9121 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag 9181 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct 9241 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc 9301 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga 9361 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg 9421 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 9481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa 9541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 9601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 9661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta 9721 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt 9781 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg 9841 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc 9901 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc 9961 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag 10021 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc 10081 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat 10141 cataatatgt acatttatat tggctcatgt ccaatatgac cgccat //
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载体质粒应用举例
pCEP4-YFP
2023-07-12pCEP4-DsRed2
2023-07-12pCEP4-Flag-N
2023-07-12
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