pAAVLINKv1-pCALM1-Cre-3'-BCOR-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-BCOR-FLAG               6936 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3649
                     /codon_start=1
                     /label=BCOR
                     /translation="VATQQPKTPRCANSAQPTGKG*KEIRTKSQSRSPWRRPLQNRTKV
                     RDASIVLETSTVIPLKPQRTKIFLWRSTLWRGSL*ASLPQTRWPRTCLTAPPSGWTGNA
                     KSQVTAATLRPLRRRCQRTLC*KPNADESLKMTGLRGK*QTVPLTT*KTHIIVS*PT*R
                     CALN*QGSILKNNATCCTLENDGSSRCRQQMANLAGKAGRK*PRPLSLRPFLRGLTSLK
                     RNLAGKGQRPKATEAGRKSLLNPVTMNKACLCSPALRP*RVFHPPVQAAKSRLSQAAHQ
                     PPGRLPNSRKLKKTRRQMCCVQTKKRIARLPPCCRNTPTTARSHPGRDCAKPNT*SLRS
                     PGGDCH*QGNTTWRMPMAR*LSGDSESGRSPVRTMICHQPSRSQSPSTACSNCYQPPSP
                     HSCHAQVPLRRPPSLALCRRKHGDLLSIRTLARPFCSGQPGLAMRKWSCTA*RTRFVM*
                     IIGTTQVTAPCMKLVLGAGSTLCDTSLNMALMSTVVPRMEPGLCTMLLRTITWKLSDYF
                     SLMVLTPPWLRTQVEPS*K*PTVNLWKSS*QII*MTSRVAMMMTPVALGTSMAALFVNQ
                     MMKVAMMF*PTPQDQKTRMMMTMPIAMCLNLNFQRPPSYRVITSKYLWLRGHETGYCFR
                     MSLRN*KCPPAYFAAIFQTWKLSPLQRQNFIGRFLQVSCSLAPKTWKPSTLKVRSC*IW
                     WNSRTKFRLCWAPL*SGSTPVIWPQTTT"
     misc_feature    1587..1592
                     /label=SDS
     misc_feature    1833..1838
                     /label=SDS
     misc_feature    2929..2934
                     /label=SDS
     CDS             3656..3721
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3725..4156
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3869..3874
                     /label=SDS
     repeat_region   4199..4339
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4199..4328
                     /label=AAV2 ITR
     rep_origin      4414..4869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5151..5255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5256..6116
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6287..6875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtggccacc cagcaaccaa
     1501 agactccgag atgtgcaaat tcagcccagc cgactgggaa aggttgaaag gaaatcagga
     1561 caaaaagcca aagtcggtca ccctggagga ggccattgca gaacagaacg aaagtgagag
     1621 atgcgagtat agtgttggaa acaagcaccg tgatcccttt gaagccccag aggacaaaga
     1681 tcttcctgtg gagaagtact ttgtggagag gcagcctgtg agcgagcctc ccgcagacca
     1741 ggtggcctcg gacatgcctc acagccccac cctccgggtg gacaggaaac gcaaagtctc
     1801 aggtgacagc agccacactg agaccactgc ggaggaggtg ccagaggacc ctctgctgaa
     1861 agccaaacgc cgacgagtct ctaaagatga ctggcctgag agggaaatga caaacagttc
     1921 ctctaaccac ttagaagacc cacattatag tgagctgacc aacctgaagg tgtgcattga
     1981 attaacaggg ctccatccta aaaaacaacg ccacttgctg caccttagag aacgatggga
     2041 gcagcaggtg tcggcagcag atggcaaacc tggccggcaa agcaggaagg aagtgaccca
     2101 ggccactcag cctgaggcca ttcctcaggg gactaacatc actgaagaga aacctggcag
     2161 gaaaagggca gaggccaaag gcaacagaag ctggtcggaa gagtctctta aacccagtga
     2221 caatgaacaa ggcttgcctg tgttctccgg ctctccgccc atgaagagtc tttcatccac
     2281 cagtgcaggc ggcaaaaagc aggctcagcc aagctgcgca ccagcctcca ggccgcctgc
     2341 caaacagcag aaaattaaag aaaaccagaa gacagatgtg ctgtgtgcag acgaagaaga
     2401 ggattgccag gctgcctccc tgctgcagaa atacaccgac aacagcgaga agccatccgg
     2461 gaagagactg tgcaaaacca aacacttgat ccctcaggag tccaggcggg gattgccact
     2521 gacaggggaa tactacgtgg agaatgccga tggcaaggtg actgtccgga gattcagaaa
     2581 gcggccggag cccagttcgg actatgatct gtcaccagcc aagcaggagc caaagccctt
     2641 cgaccgcttg cagcaactgc taccagcctc ccagtccaca cagctgccat gctcaagttc
     2701 ccctcaggag accacccagt ctcgccctat gccgccggaa gcacggagac ttattgtcaa
     2761 taagaacgct ggcgagaccc ttctgcagcg ggcagccagg cttggctatg aggaagtggt
     2821 cctgtactgc ttagagaaca agatttgtga tgtaaatcat cgggacaacg caggttactg
     2881 cgccctgcat gaagcttgtg ctaggggctg gctcaacatt gtgcgacacc tccttgaata
     2941 tggcgctgat gtcaactgta gtgcccagga tggaaccagg cctctgcacg atgctgttga
     3001 gaacgatcac ttggaaattg tccgactact tctctcttat ggtgctgacc ccaccttggc
     3061 tacgtactca ggtagaacca tcatgaaaat gacccacagt gaacttatgg aaaagttctt
     3121 aacagattat ttaaatgacc tccagggtcg caatgatgat gacgccagtg gcacttggga
     3181 cttctatggc agctctgttt gtgaaccaga tgatgaaagt ggctatgatg ttttagccaa
     3241 ccccccagga ccagaagacc aggatgatga tgacgatgcc tatagcgatg tgtttgaatt
     3301 tgaattttca gagacccccc tcttaccgtg ttataacatc caagtatctg tggctcaggg
     3361 gccacgaaac tggctactgc tttcggatgt ccttaagaaa ttgaaaatgt cctcccgcat
     3421 atttcgctgc aattttccaa acgtggaaat tgtcaccatt gcagaggcag aattttatcg
     3481 gcaggtttct gcaagtctct tgttctcttg ctccaaagac ctggaagcct tcaaccctga
     3541 aagtaaggag ctgttagatc tggtggaatt cacgaacgaa attcagactc tgctgggctc
     3601 ctctgtagag tggctccacc ccagtgatct ggcctcagac aactactggt gtacagacta
     3661 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     3721 gtaactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3781 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3841 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
     3901 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3961 actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4021 atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4081 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4141 gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag
     4201 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4261 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4321 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4381 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4441 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     4501 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4561 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4621 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4681 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4741 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     4801 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4861 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4921 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4981 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5041 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5101 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6901 ggttcctggc cttttgctgg ccttttgctc acatgt
//