pREP10

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pREP10
质粒类型: 哺乳动物表达载体;附加体载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: RSV
载体大小: 9287bp
5' 测序引物及序列: pREP Forward 5’ GCTCGATACAATAAACGCC 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α
宿主细胞(系): 人类细胞;灵长类细胞;犬类细胞
备注:
pREP7载体是附加体型载体,可以在基因组外自主复制,类似的载体有:
   pREP4
   PCEP4
   pREP7
   pREP10
   pEBNA-DEST
   pEBVHisA
   pEBVHisB
   pEBVHisC
   pREP4/CAT
   pEBVHis-lacZ
 
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pREP10载体图谱



pREP10多克隆位点

载体简介

载体序列

LOCUS       Exported                9287 bp ds-DNA     circular SYN 12-AUG-2016
DEFINITION  synthetic circular DNA
KEYWORDS    pREP10
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Biofeng Lab.
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    275..409
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(476..737)
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     polyA_signal    1503..1551
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1593..2630)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(2673..2818)
                     /note="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(3249..3837)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4008..4868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4869..4973)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      7049..8837
                     /note="oriP"
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
       61 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag
      181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg gtaccagctg
      421 aagcttgcta gcggccgctc gaggccggca aggccggatc cgtacccagc ttggaggtgc
      481 acaccaatgt ggtgaatggt caaatggcgt ttattgtatc gagctaggca cttaaataca
      541 atatctctgc aatgcggaat tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc
      601 ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca cggtgctttt
      661 tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac tacaagagta
      721 ttgcataaga ctacatttcc ccctccctat gcaaaagcga aactactata tcctgagggg
      781 actcctaacc gcgtacaacc gaagccccgc ttttcgccta aacacaccct agtcccctca
      841 gatacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag
      901 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca
      961 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc
     1021 agagcagatt gtactgagag tgcaccatac ggatctgcga tgataagctg tcaaacatga
     1081 gaattggtcg actagcttgg cacgccagaa atccgcgcgg tggtttttgg gggtcggggg
     1141 tgtttggcag ccacagacgc ccggtgttcg tgtcgcgcca gtacatgcgg tccatgccca
     1201 ggccatccaa aaaccatggg tctgtctgct cagtccagtc gtggacctga ccccacgcaa
     1261 cgcccaaaat aataaccccc acgaaccata aaccattccc catgggggac cccgtcccta
     1321 acccacgggg ccagtggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     1381 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     1441 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     1501 atgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc
     1561 gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccgatcc
     1621 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     1681 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     1741 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     1801 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct
     1861 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     1921 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     1981 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     2041 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     2101 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     2161 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     2221 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     2281 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
     2341 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc
     2401 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
     2461 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
     2521 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
     2581 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc
     2641 cccccgggat ctgcggcacg ctgttgacgc tgttaagcgg gtcgctgcag ggtcgctcgg
     2701 tgttcgaggc cacacgcgtc accttaatat gcgaagtgga cctcggaccg cgccgccccg
     2761 actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca
     2821 tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac
     2881 gggccacggg gatgaagcag ggcggcacct cgctaacgga ttcaccactc caagaattgg
     2941 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
     3001 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcggggccga cgcgctgggc
     3061 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     3121 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     3181 gaccatcagg gacagcttca aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3661 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4321 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4621 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4981 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     5041 aataggcgta tcacgaggcc ctttcgtctt caagaattct catgtttgac agcttatcat
     5101 cgataagctg atcctcacag gccgcaccca gcttttcttc cgttgcccca gtagcatctc
     5161 tgtctggtga ccttgaagag gaagaggagg ggtcccgaga atccccatcc ctaccgtcca
     5221 gcaaaaaggg ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca aatcttgagg
     5281 atgttcagcg ggagttttcc gggctgcgag taattggtga tgaggacgag gatggttcgg
     5341 aggatgggga attttcagac ctggatctgt ctgacagcga ccatgaaggg gatgagggtg
     5401 ggggggctgt tggagggggc aggagtctgc actccctgta ttcactgagc gtcgtctaat
     5461 aaagatgtct attgatctct tttagtgtga atcatgtctg acgaggggcc aggtacagga
     5521 cctggaaatg gcctaggaga gaagggagac acatctggac cagaaggctc cggcggcagt
     5581 ggacctcaaa gaagaggggg tgataaccat ggacgaggac ggggaagagg acgaggacga
     5641 ggaggcggaa gaccaggagc cccgggcggc tcaggatcag ggccaagaca tagagatggt
     5701 gtccggagac cccaaaaacg tccaagttgc attggctgca aagggaccca cggtggaaca
     5761 ggagcaggag caggagcggg aggggcagga gcaggaggtg gaggccgggg tcgaggaggc
     5821 agtggaggcc ggggtcgagg aggtagtgga ggccggggtc gaggaggtag tggaggccgc
     5881 cggggtagag gacgtgaaag agccaggggg ggaagtcgtg aaagagccag ggggagaggt
     5941 cgtggacgtg gagaaaagag gcccaggagt cccagtagtc agtcatcatc atccgggtct
     6001 ccaccgcgca ggccccctcc aggtagaagg ccatttttcc accctgtagg ggaagccgat
     6061 tattttgaat accaccaaga aggtggccca gatggtgagc ctgacgtgcc cccgggagcg
     6121 atagagcagg gccccgcaga tgacccagga gaaggcccaa gcactggacc ccggggtcag
     6181 ggtgatggag gcaggcgcaa aaaaggaggg tggtttggaa agcatcgtgg tcaaggaggt
     6241 tccaacccga aatttgagaa cattgcagaa ggtttaagag ctctcctggc taggagtcac
     6301 gtagaaagga ctaccgacga aggaacttgg gtcgccggtg tgttcgtata tggaggtagt
     6361 aagacctccc tttacaacct aaggcgagga actgcccttg ctattccaca atgtcgtctt
     6421 acaccattga gtcgtctccc ctttggaatg gcccctggac ccggcccaca acctggcccg
     6481 ctaagggagt ccattgtctg ttatttcatg gtctttttac aaactcatat atttgctgag
     6541 gttttgaagg atgcgattaa ggaccttgtt atgacaaagc ccgctcctac ctgcaatatc
     6601 agggtgactg tgtgcagctt tgacgatgga gtagatttgc ctccctggtt tccacctatg
     6661 gtggaagggg ctgccgcgga gggtgatgac ggagatgacg gagatgaagg aggtgatgga
     6721 gatgagggtg aggaagggca ggagtgatgt aacttgttag gagacgccct caatcgtatt
     6781 aaaagccgtg tattcccccg cactaaagaa taaatcccca gtagacatca tgcgtgctgt
     6841 tggtgtattt ctggccatct gtcttgtcac cattttcgtc ctcccaacat ggggcaattg
     6901 ggcataccca tgttgtcacg tcactcagct ccgcgctcaa caccttctcg cgttggaaaa
     6961 cattagcgac atttacctgg tgagcaatca gacatgcgac ggctttagcc tggcctcctt
     7021 aaattcacct aagaatggga gcaaccagca ggaaaaggac aagcagcgaa aattcacgcc
     7081 cccttgggag gtggcggcat atgcaaagga tagcactccc actctactac tgggtatcat
     7141 atgctgactg tatatgcatg aggatagcat atgctacccg gatacagatt aggatagcat
     7201 atactaccca gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct
     7261 atgctaccca gatataaatt aggatagcat atactaccca gatatagatt aggatagcat
     7321 atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt aggatagcat
     7381 atgctaccca gatatagatt aggatagcat atgctatcca gatatttggg tagtatatgc
     7441 tacccagata taaattagga tagcatatac taccctaatc tctattagga tagcatatgc
     7501 tacccggata cagattagga tagcatatac tacccagata tagattagga tagcatatgc
     7561 tacccagata tagattagga tagcctatgc tacccagata taaattagga tagcatatac
     7621 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc
     7681 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc
     7741 catggcaaca ttagcccacc gtgctctcag cgacctcgtg aatatgagga ccaacaaccc
     7801 tgtgcttggc gctcaggcgc aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc
     7861 ccctatcttg gcccgcccac ctacttatgc aggtattccc cggggtgcca ttagtggttt
     7921 tgtgggcaag tggtttgacc gcagtggtta gcggggttac aatcagccaa gttattacac
     7981 ccttatttta cagtccaaaa ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc
     8041 cacaatttca aaaaaaagag tggccacttg tctttgttta tgggccccat tggcgtggag
     8101 ccccgtttaa ttttcggggg tgttagagac aaccagtgga gtccgctgct gtcggcgtcc
     8161 actctctttc cccttgttac aaatagagtg taacaacatg gttcacctgt cttggtccct
     8221 gcctgggaca catcttaata accccagtat catattgcac taggattatg tgttgcccat
     8281 agccataaat tcgtgtgaga tggacatcca gtctttacgg cttgtcccca ccccatggat
     8341 ttctattgtt aaagatattc agaatgtttc attcctacac tagtatttat tgcccaaggg
     8401 gtttgtgagg gttatattgg tgtcatagca caatgccacc actgaacccc ccgtccaaat
     8461 tttattctgg gggcgtcacc tgaaaccttg ttttcgagca cctcacatac accttactgt
     8521 tcacaactca gcagttattc tattagctaa acgaaggaga atgaagaagc aggcgaagat
     8581 tcaggagagt tcactgcccg ctccttgatc ttcagccact gcccttgtga ctaaaatggt
     8641 tcactaccct cgtggaatcc tgaccccatg taaataaaac cgtgacagct catggggtgg
     8701 gagatatcgc tgttccttag gaccctttta ctaaccctaa ttcgatagca tatgcttccc
     8761 ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta ctacccggga
     8821 agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt gctaatgccc
     8881 tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt ggtgtagcct
     8941 cccgtagtct tcctgggccc ctgggaggta catgtccccc agcattggtg taagagcttc
     9001 agccaagagt tacacataaa ggcaatgttg tgttgcagtc cacagactgc aaagtctgct
     9061 ccaggatgaa agccactcag tgttggcaaa tgtgcacatc catttataag gatgtcaact
     9121 acagtcagag aacccctttg tgtttggtcc ccccccgtgt cacatgtgga acagggccca
     9181 gttggcaagt tgtaccaacc aactgaaggg attacatgca ctgccccgcg aagaaggggc
     9241 agagatgtcg tagtcaggtt tagttcgtcc ggggcgggga tcgatcc
//

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