pH-nCas9-PBE

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: pH-nCas9-PBE 
出品公司: 爱迪基因
目录编号: 98163
存储实验室: Caixia Gao

载体骨架基本信息
载体名称: pHUE411
载体出品公司: --
载体抗性: 卡那霉素
空载体大小: --
完整质粒大小: --
载体修饰: --
载体类型: 植物表达;水稻
筛选标记: 潮霉素
高拷贝/低拷贝: --
生长条件: 37 ℃

插入基因信息
插入基因名称: NLS-APOBEC1-XTEN-nCas9-UGI-NLS
物种来源: --
插入大小: --
基因突变: --
GenBank编号 --
启动子 Ubi
标签/融合蛋白: --
克隆方法: Gibson克隆
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: pH-nCas9-PBE was a gift from Caixia Gao (Addgene plasmid # 98163 ; http://n2t.net/addgene:98163 ; RRID:Addgene_98163)
参考文献部分: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. 10.1038/nbt.3811 PubMed 28244994

载体图谱质粒图谱和多克隆位点信息

pH-nCas9-PBE质粒图谱

载体简介

载体序列

LOCUS       pH-nCas9-PBE          19438 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Expresses CRISPR base editor in rice.
ACCESSION   .
VERSION     .
KEYWORDS    pH-nCas9-PBE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19438)
  AUTHORS   Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C
  TITLE     Precise base editing in rice, wheat and maize with a Cas9-cytidine 
            deaminase fusion.
  JOURNAL   Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 
            2017 Feb 27.
  PUBMED    28244994
REFERENCE   2  (bases 1 to 19438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19438
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             999..1019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1020..1703
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             1755..5855
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5856..5903
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5925..6173
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6186..6206
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6864..6880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6864..6880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6877..6899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6888..6904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6912..6942)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6957..6978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7169..7845
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     7782..7802
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             7912..8937
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    8978..9152
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9230..9254
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     mobile_element  9688..10455
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     CDS             10456..11250
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11337..11925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11826..11845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12079..12096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12111..12251
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12177..12196)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12337..12359
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12595..12789
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12855..13928)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14357..14986)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    16287..16311
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     16499..16521
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16513..16530
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16514..16530
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        16597..16976
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             17256..18047
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        18199..18274
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ccccccctct ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc
       61 tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc
      121 gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc
      181 tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt
      241 tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca
      301 cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg
      361 gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt
      421 aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga
      481 tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca
      541 gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg
      601 ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact
      661 gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct
      721 aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag
      781 catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta
      841 taattatttt gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt
      901 ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc
      961 accctgttgt ttggtgttac ttctgcagcc ctaggatgcc aaagaagaag aggaaggttt
     1021 catcggagac cggccctgtt gctgttgacc ccaccctgcg gcggagaatc gagccacacg
     1081 agttcgaggt gttcttcgac ccaagggagc tccgcaagga gacgtgcctc ctgtacgaga
     1141 tcaactgggg cggcaggcac tccatctgga ggcacaccag ccaaaacacc aacaagcacg
     1201 tggaggtcaa cttcatcgag aagttcacca ccgagaggta cttctgccca aacacccgct
     1261 gctccatcac ctggttcctg tcctggagcc catgcggcga gtgctccagg gccatcaccg
     1321 agttcctcag ccgctaccca cacgtcaccc tgttcatcta catcgccagg ctctaccacc
     1381 acgccgaccc aaggaacagg cagggcctcc gcgacctgat ctccagcggc gtgaccatcc
     1441 aaatcatgac cgagcaggag tccggctact gctggaggaa cttcgtcaac tactccccaa
     1501 gcaacgaggc ccactggcca aggtacccac acctctgggt gcgcctctac gtgctcgagc
     1561 tgtactgcat catcctcggc ctgccaccat gcctcaacat cctgaggcgc aagcaaccac
     1621 agctgacctt cttcaccatc gccctccaaa gctgccacta ccagaggctc ccaccacaca
     1681 tcctgtgggc taccggcctc aagtccggca gcgagacgcc aggcacctcc gagagcgcta
     1741 cgcctgaact taaggacaag aagtactcga tcggcctcgc catcgggacg aactcagttg
     1801 gctgggccgt gatcaccgac gagtacaagg tgccctctaa gaagttcaag gtcctgggga
     1861 acaccgaccg ccattccatc aagaagaacc tcatcggcgc tctcctgttc gacagcgggg
     1921 agaccgctga ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga
     1981 acaggatctg ctacctccaa gagattttct ccaacgagat ggccaaggtt gacgattcat
     2041 tcttccaccg cctggaggag tctttcctcg tggaggagga taagaagcac gagcggcatc
     2101 ccatcttcgg caacatcgtg gacgaggttg cctaccacga gaagtaccct acgatctacc
     2161 atctgcggaa gaagctcgtg gactccaccg ataaggcgga cctcagactg atctacctcg
     2221 ctctggccca catgatcaag ttccgcggcc atttcctgat cgagggggat ctcaacccag
     2281 acaacagcga tgttgacaag ctgttcatcc aactcgtgca gacctacaac caactcttcg
     2341 aggagaaccc gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct
     2401 cgaagtccag gaggctggag aacctgatcg ctcagctccc aggcgagaag aagaacggcc
     2461 tgttcgggaa cctcatcgct ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg
     2521 atctcgctga ggacgccaag ctgcaactct ccaaggacac ctacgacgat gacctcgata
     2581 acctcctggc ccagatcggc gatcaatacg cggacctgtt cctcgctgcc aagaacctgt
     2641 cggacgccat cctcctgtca gatatcctcc gcgtgaacac cgagatcacg aaggctccac
     2701 tctctgcctc catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg
     2761 cgctggtccg ccaacagctc ccggagaagt acaaggagat tttcttcgat cagtcgaaga
     2821 acggctacgc tgggtacatc gacggcgggg cctcacaaga ggagttctac aagttcatca
     2881 agccaatcct ggagaagatg gacggcacgg aggagctcct ggtgaagctc aacagggagg
     2941 acctcctgcg gaagcagaga accttcgata acggcagcat cccccaccaa atccatctcg
     3001 gggagctgca cgccatcctg agaaggcaag aggacttcta ccctttcctc aaggataacc
     3061 gggagaagat cgagaagatc ctgaccttca gaatcccata ctacgtcggc cctctcgcgc
     3121 gggggaactc aagattcgct tggatgaccc gcaagtctga ggagaccatc acgccgtgga
     3181 acttcgagga ggtggtggac aagggcgcta gcgctcagtc gttcatcgag aggatgacca
     3241 acttcgacaa gaacctgccc aacgagaagg tgctccctaa gcactcgctc ctgtacgagt
     3301 acttcaccgt ctacaacgag ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc
     3361 cagcgttcct gtccggggag cagaagaagg ctatcgtgga cctcctgttc aagaccaacc
     3421 ggaaggtcac ggttaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgatt
     3481 cggtcgagat cagcggcgtt gaggaccgct tcaacgccag cctcgggacc taccacgatc
     3541 tcctgaagat catcaaggat aaggacttcc tggacaacga ggagaacgag gatatcctgg
     3601 aggacatcgt gctgaccctc acgctgttcg aggacaggga gatgatcgag gagcgcctga
     3661 agacgtacgc ccatctcttc gatgacaagg tcatgaagca actcaagcgc cggagataca
     3721 ccggctgggg gaggctgtcc cgcaagctca tcaacggcat ccgggacaag cagtccggga
     3781 agaccatcct cgacttcctc aagagcgatg gcttcgccaa caggaacttc atgcaactga
     3841 tccacgatga cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg agcggccagg
     3901 gggactcgct gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc aagaagggca
     3961 tcctccagac cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg cacaagcctg
     4021 agaacatcgt catcgagatg gccagagaga accaaaccac gcagaagggg caaaagaact
     4081 ctagggagcg catgaagcgc atcgaggagg gcatcaagga gctggggtcc caaatcctca
     4141 aggagcaccc agtggagaac acccaactgc agaacgagaa gctctacctg tactacctcc
     4201 agaacggcag ggatatgtac gtggaccaag agctggatat caaccgcctc agcgattacg
     4261 acgtcgatca tatcgttccc cagtctttcc tgaaggatga ctccatcgac aacaaggtcc
     4321 tcaccaggtc ggacaagaac cgcggcaagt cagataacgt tccatctgag gaggtcgtta
     4381 agaagatgaa gaactactgg aggcagctcc tgaacgccaa gctgatcacg caaaggaagt
     4441 tcgacaacct caccaaggct gagagaggcg ggctctcaga gctggacaag gccggcttca
     4501 tcaagcggca gctggtcgag accagacaaa tcacgaagca cgttgcgcaa atcctcgact
     4561 ctcggatgaa cacgaagtac gatgagaacg acaagctgat cagggaggtt aaggtgatca
     4621 ccctgaagtc taagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcgcg
     4681 agatcaacaa ctaccaccat gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc
     4741 tgatcaagaa gtacccaaag ctggagtccg agttcgtgta cggggactac aaggtttacg
     4801 atgtgcgcaa gatgatcgcc aagtcggagc aagagatcgg caaggctacc gccaagtact
     4861 tcttctactc aaacatcatg aacttcttca agaccgagat cacgctggcc aacggcgaga
     4921 tccggaagag accgctcatc gagaccaacg gcgagacggg ggagatcgtg tgggacaagg
     4981 gcagggattt cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga
     5041 agaccgaggt ccaaacgggc gggttctcaa aggagtctat cctgcctaag cggaacagcg
     5101 acaagctcat cgccagaaag aaggactggg acccaaagaa gtacggcggg ttcgacagcc
     5161 ctaccgtggc ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc
     5221 tcaagagcgt gaaggagctc ctggggatca ccatcatgga gaggtccagc ttcgagaaga
     5281 acccaatcga cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca
     5341 agctcccgaa gtactctctc ttcgagctgg agaacggcag gaagagaatg ctggcttccg
     5401 ctggcgagct ccagaagggg aacgagctcg cgctgccaag caagtacgtg aacttcctct
     5461 acctggcttc ccactacgag aagctcaagg gcagcccgga ggacaacgag caaaagcagc
     5521 tgttcgtcga gcagcacaag cattacctcg acgagatcat cgagcaaatc tccgagttca
     5581 gcaagcgcgt gatcctcgcc gacgcgaacc tggataaggt cctctccgcc tacaacaagc
     5641 accgggacaa gcccatcaga gagcaagcgg agaacatcat ccatctcttc accctgacga
     5701 acctcggcgc tcctgctgct ttcaagtact tcgacaccac gatcgatcgg aagagataca
     5761 cctccacgaa ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg
     5821 agacgaggat cgacctctca caactcggcg gggataagag acccgcagca accaagaagg
     5881 cagggcaagc aaagaagaag aagacgcgtg actccggcgg cagcaccaac ctgtccgaca
     5941 tcatcgagaa ggagacgggc aagcaactcg tgatccagga gagcatcctc atgctgccag
     6001 aggaggtgga ggaggtcatc ggcaacaagc cagagtccga catcctggtg cacaccgcct
     6061 acgacgagtc caccgacgag aacgtcatgc tcctgaccag cgacgcccca gagtacaagc
     6121 catgggccct cgtcatccag gacagcaacg gggagaacaa gatcaagatg ctgtcggggg
     6181 ggagcccaaa gaagaagcgg aaggtgtaga gctcagagct ttcgttcgta tcatcggttt
     6241 cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg cacacaccag aatcctactg
     6301 agtttgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     6361 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     6421 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     6481 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     6541 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacacttg
     6601 tagttgtacc attatgctta ttcactaggc aacaaatata ttttcagacc tagaaaagct
     6661 gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta gacatttatg aactttcctt
     6721 tatgtaattt tccagaatcc ttgtcagatt ctaatcattg ctttataatt atagttatac
     6781 tcatggattt gtagttgagt atgaaaatat tttttaatgc attttatgac ttgccaattg
     6841 attgacaacg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6901 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6961 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7021 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc
     7081 tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
     7141 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
     7201 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
     7261 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
     7321 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     7381 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
     7441 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
     7501 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     7561 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
     7621 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     7681 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     7741 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     7801 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc
     7861 tctacaaatc tatctctctc gagctttcgc agatccgggg ggcaatgaga tatgaaaaag
     7921 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     7981 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     8041 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     8101 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggagttt
     8161 agcgagagcc tgacctattg catctcccgc cgttcacagg gtgtcacgtt gcaagacctg
     8221 cctgaaaccg aactgcccgc tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct
     8281 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     8341 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     8401 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     8461 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     8521 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     8581 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     8641 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc acgactccgg
     8701 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     8761 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     8821 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     8881 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa gaaatagagt
     8941 agatgccgac cgggatctgt cgatcgacaa gctcgagttt ctccataata atgtgtgagt
     9001 agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa
     9061 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     9121 taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt
     9181 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     9241 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     9301 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     9361 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     9421 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     9481 tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     9541 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     9601 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     9661 agaggaagga aataataaat ggctaaaggt aatgactcca acttattgat agtgttttat
     9721 gttcagataa tgcccgatga ctttgtcatg cagctccacc gattttgaga acgacagcga
     9781 cttccgtccc agccgtgcca ggtgctgcct cagattcagg ttatgccgct caattcgctg
     9841 cgtatatcgc ttgctgatta cgtgcagctt tcccttcagg cgggattcat acagcggcca
     9901 gccatccgtc atccatatca ccacgtcaaa gggtgacagc aggctcataa gacgccccag
     9961 cgtcgccata gtgcgttcac cgaatacgtg cgcaacaacc gtcttccgga gactgtcata
    10021 cgcgtaaaac agccagcgct ggcgcgattt agccccgaca tagccccact gttcgtccat
    10081 ttccgcgcag acgatgacgt cactgcccgg ctgtatgcgc gaggttaccg actgcggcct
    10141 gagtttttta agtgacgtaa aatcgtgttg aggccaacgc ccataatgcg ggctgttgcc
    10201 cggcatccaa cgccattcat ggccatatca atgattttct ggtgcgtacc gggttgagaa
    10261 gcggtgtaag tgaactgcag ttgccatgtt ttacggcagt gagagcagag atagcgctga
    10321 tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca ggagggacag
    10381 ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact aaatcagtaa
    10441 gttggcagca tcaccatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
    10501 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
    10561 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
    10621 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
    10681 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
    10741 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
    10801 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
    10861 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
    10921 tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta
    10981 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
    11041 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
    11101 gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa
    11161 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
    11221 tattatattt tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa
    11281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    11341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    11401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    11461 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
    11521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11941 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    12001 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    12061 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
    12121 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
    12181 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
    12241 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
    12301 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
    12361 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
    12421 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
    12481 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
    12541 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
    12601 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
    12661 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
    12721 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
    12781 ctatccacag gaaacagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
    12841 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
    12901 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
    12961 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
    13021 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
    13081 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
    13141 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
    13201 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
    13261 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
    13321 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
    13381 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
    13441 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
    13501 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
    13561 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
    13621 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
    13681 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
    13741 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
    13801 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
    13861 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
    13921 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcaggcaa
    13981 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    14041 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    14101 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    14161 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    14221 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    14281 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    14341 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    14401 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    14461 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    14521 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    14581 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    14641 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    14701 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    14761 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    14821 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    14881 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    14941 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    15001 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    15061 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    15121 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    15181 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    15241 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    15301 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    15361 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    15421 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    15481 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    15541 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    15601 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    15661 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    15721 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    15781 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    15841 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    15901 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    15961 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    16021 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    16081 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    16141 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    16201 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
    16261 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
    16321 agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct ctagcattcg
    16381 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    16441 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    16501 cagtcacgac gttgtaaaac gacggccagt gccaagctta gtaattcatc caggtctcca
    16561 agttctagga ttttcagaac tgcaacttat tttatcaagg aatctttaaa catacgaaca
    16621 gatcacttaa agttcttctg aagcaactta aagttatcag gcatgcatgg atcttggagg
    16681 aatcagatgt gcagtcaggg accatagcac aagacaggcg tcttctactg gtgctaccag
    16741 caaatgctgg aagccgggaa cactgggtac gttggaaacc acgtgatgtg aagaagtaag
    16801 ataaactgta ggagaaaagc atttcgtagt gggccatgaa gcctttcagg acatgtattg
    16861 cagtatgggc cggcccatta cgcaattgga cgacaacaaa gactagtatt agtaccacct
    16921 cggctatcca catagatcaa agctgattta aaagagttgt gcagatgatc cgtggcgtga
    16981 gaccaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
    17041 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
    17101 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
    17161 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
    17221 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
    17281 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
    17341 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
    17401 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
    17461 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
    17521 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
    17581 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
    17641 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
    17701 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
    17761 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
    17821 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
    17881 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
    17941 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
    18001 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
    18061 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
    18121 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
    18181 cataataagc cggtctcggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
    18241 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt ttttcgtttt gcattgagtt
    18301 ttctccgtcg catgtttgca gttttatttt ccgttttgca ttgaaatttc tccgtctcat
    18361 gtttgcagcg tgttcaaaaa gtacgcagct gtatttcact tatttacggc gccacatttt
    18421 catgccgttt gtgccaacta tcccgagcta gtgaatacag cttggcttca cacaacactg
    18481 gtgacccgct gacctgctcg tacctcgtac cgtcgtacgg cacagcattt ggaattaaag
    18541 ggtgtgatcg atactgcttg ctgctaagct tgcatgcctg cagtgcagcg tgacccggtc
    18601 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
    18661 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
    18721 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata
    18781 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac
    18841 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata
    18901 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata
    18961 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa
    19021 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga
    19081 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt
    19141 ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag
    19201 cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc
    19261 ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca
    19321 gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct
    19381 cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcct
//

载体图谱质粒图谱pdf版和相关资料下载

载体质粒应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng 沪ICP备2021037978号-1
欢迎您的访问和咨询....