| 质粒名称: | pAAV-AscI-CAG-NES-NeMeHg |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000572 |
| 产品用途: | Expresses NeMeHg in mammalian cell culture |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | NeMeHg |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
NeMeHg, genetically encoded indicator for mercury ions based on mNeonGreen green fluorescent protein and merP protein from Shigella flexneri
Subach O.M., Piatkevich K.D., Subach F.V.
Front. Bioeng. Biotechnol. 12, 1407874 (2024)
|
pAAV-AscI-CAG-NES-NeMeHg
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-AscI-CAG-NES-NeMeHg载体质粒基本信息
pAAV-AscI-CAG-NES-NeMeHg质粒图谱载体图谱和pAAV-AscI-CAG-NES-NeMeHg载体序列质粒序列多克隆位点信息
pAAV-AscI-CAG-NES-NeMeHg质粒载体简介
pAAV-AscI-CAG-NES-NeMeHg质粒序列载体序列
LOCUS pAAV-AscI-CAG-NES-NeMeHg 5762 bp DNA circular SYN 24-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5762)
AUTHORS 1
TITLE Direct Submission
JOURNAL Exported Jul 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..5762
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
misc_feature 2660..2674
/label=AscI-OxE insert
/label=AscI-OxE\insert
/note="/vntifkey=21"
misc_feature 2675..2918
/label=CMV enhancer
/label=CMV\enhancer
/note="/vntifkey=21"
misc_feature 2925..3202
/label=chicken b-actin promoter
/label=chicken\b-actin\promoter
/note="/vntifkey=21"
promoter 2925..3202
/label=chicken beta-actin promoter
primer_bind complement(3575..3601)
/label=172-WKG-572-R
misc_feature 3626..3661
/label=NES
/note="/vntifkey=21"
CDS 3626..3661
/codon_start=1
/label=NES
/note="/vntifkey=21"
/translation="LQLPPLERLTLS"
misc_feature 3635..3640
/label=SDS
misc_feature 3668..4639
/label=NeMeHg
/note="/vntifkey=21"
misc_feature 4664..5252
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
misc_feature 4800..4805
/label=SDS
CDS complement(5135..5146)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5295..5416
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature join(5762,1..940)
/label=ColE1
/note="ColE1"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagtcgtag gttaattaag gcgcgccgcc taggccattg acgtcaataa tgacgtatgt
2701 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
2761 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
2821 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
2881 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
2941 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
3001 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg
3061 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
3121 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
3181 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
3241 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
3301 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
3361 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
3421 gagcggctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
3481 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
3541 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
3601 gcaaagaatt ggatccgaca ccatgcttca acttcctcct cttgaacgtc ttactctttc
3661 gagatctatg gctacccaga ccgtcacact agcggttccc ggcatgactt gcgccgcctg
3721 cccgatcaca gtcaagaaag cgctctccaa ggtcgaaggc gtgagcaagg tcgatgtggg
3781 cttcgagaat cctgctgact ggcgtatatc caagaaggct tgccccaatg gtaagactct
3841 catttccaca cttaagtggt cttataccac cggcaacggc aaacgctacc ggtctactgc
3901 tcgcaccaca tacacattcg ccaaaccaat ggccgcaaat tacctcaaaa atcagccgat
3961 gtatgtcttt cggaaaacag agctgaaaca ttctaagacc gaaatggatt ttaaagagtg
4021 gcaaaaggcc tttacggacg tgatgggcat ggacgagctg tataaggtgg atggcggcag
4081 cggtggcacc ggcatggtct cagagggaga gggggacaac atggctagtc tgcctgctac
4141 ccacgaactg catatttttg gctccataaa tggcgtcgat ttcgacatgg taggccaagg
4201 ctccggtaat ccaaacgtcg gttatgagga gctgaacctg aagtccacca agggcgatct
4261 gcagttcagt ccctggatcc tggtgccgca cattgggtac gggtttcatc agtatctccc
4321 ctacccggac gggatgtcac catttcaggc tgcaatggtc gacgggtccg gatatcaggt
4381 gcaccgcact gtgcagtttg aggacggtgc ttcactgacc gtgaattaca gatacaccta
4441 cgaaggatca catatcaaag gggaagctca ggtgaagggc actgggttcc cggcagacgg
4501 ccctgtgatg acaaattccc tgacagcatt tgtttgtaag cgcgaggtca tcgtcacttt
4561 tgacgacacc aaggccagcg tacagaagct gaccagggcc accgcagacg ccggctatcc
4621 gtccagcgtc aagcagtgag aattcgatat caagcttatc gataatcaac ctctggatta
4681 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
4741 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
4801 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
4861 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
4921 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
4981 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
5041 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
5101 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
5161 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
5221 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct
5281 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
5341 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
5401 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
5461 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
5521 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
5581 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
5641 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
5701 cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
5761 ct
//
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