| 质粒名称: | pVS1-SAH2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 134746 |
| 存储实验室: | Qi-Jun Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pVS1 |
| 完整质粒大小: | 13322bp |
| 载体类型: | Helper plasmid |
| 载体抗性: | Spectinomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | trfA-trfB-parDE |
| 物种来源: | 未知 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pVS1-SAH2 was a gift from Qi-Jun Chen (Addgene plasmid # 134746 ; http://n2t.net/addgene:134746 ; RRID:Addgene_134746) |
| 参考文献部分: | A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. 10.1104/pp.19.00767 PubMed 31558579 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVS1-SAH2 13322 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Replication helper.
ACCESSION .
VERSION .
KEYWORDS pVS1-SAH2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13322)
AUTHORS Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
TITLE A Novel Ternary Vector System United with Morphogenic Genes Enhances
CRISPR/Cas Delivery in Maize.
JOURNAL Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi:
10.1104/pp.19.00767.
PUBMED 31558579
REFERENCE 2 (bases 1 to 13322)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..13322
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 278..1069
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1325..1913
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1814..1833
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2067..2084
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2099..2239
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2165..2184)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2325..2347
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 2583..2777
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(2843..3916)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(4345..4974)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 8025..9173
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
1 aattcgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
1201 tgcataataa gcccaaggcg cgcctacaca aattgggaga tatatcatgc atgaccaaaa
1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
2521 agcacagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
3901 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
3961 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
4021 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
4081 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
4141 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
4201 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
4261 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
4321 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
4381 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
4441 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
4501 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
4561 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
4621 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
4681 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
4741 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
4801 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
4861 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
4921 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
4981 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
5041 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
5101 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
5161 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
5221 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
5281 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
5341 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
5401 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
5461 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
5521 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
5581 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
5641 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
5701 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
5761 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
5821 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
5881 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
5941 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
6001 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
6061 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
6121 cgactgcact atagcttaat tcatcgcgtc ccccttggtc aaattgggta tacccatttg
6181 ggcctagtct agccggcatg gcgcattaca gcaatacgca atttaaatgc gcctagcgca
6241 ttttcccgac cttaatgcgc ctcgcgctgt agcctcacgc ccacatatgt gctaatgtgg
6301 ttacgtgtat tttatggagg ttatccaatg agccgcctga caatcgacat gacggaccag
6361 cagcaccaga gcctgaaagc cctggccgcc ttgcagggca agaccattaa gcaatacgcc
6421 ctcgaacgtc tgttccccgg tgacgctgat gccgatcagg catggcagga actgaaaacc
6481 atgctgggga accgcatcaa cgatgggctt gccggcaagg tgtccaccaa gagcgtcggc
6541 gaaattcttg atgaagaact cagcggggat cgcgcttgac ggcctacatc ctcacggctg
6601 aggccgaagc cgatctacgc ggcatcatcc gctacacgcg ccgggagtgg ggcgcggcgc
6661 aggtgcgccg ctatatcgct aagctggaac agggcatagc caggcttgcc gccggcgaag
6721 gcccgtttaa ggacatgagc gaactctttc ccgcgctgcg gatggcccgc tgcgaacacc
6781 actacgtttt ttgcctgccg cgtgcgggcg aacccgcgtt ggtcgtggcg atcctgcatg
6841 agcgcatgga cctcatgacg cgacttgccg acaggctcaa gggctgattt cagccgctaa
6901 aaatcgcgcc actcacaacg tcctgatggc gtacttaatt aacgacttta ggtccaagct
6961 taatcgaacc gcacgacgta ggggttctga tagaaaacaa ctgtcaaagc gcacccgccc
7021 gatgccattc gcggcacggc ttccgttgag gatgtcgata tgatgcgcga gccgacggcc
7081 cgcagagaag gggccgtttt agcggctaaa gaaggaagtg caagccctaa cccttggcgt
7141 cagagccttc cacgcagctt ttttcgggtg tcgtcgcccc atttctttac gataaacgcc
7201 ttatgtgacg gcaaaaccac actgatgcgt tcgtatccgg gcggcacgct gctcttgaaa
7261 ggatgacccg caatctccgc gagtgcctcg cggtcaaggt cggtggactc caggagaaga
7321 ggtaggggag tttccagggc gtcggcaatg gcctccatca ccttcaacga ggggttggcc
7381 ttaccgttgg ttaagtctga taaaaacgaa attgaaaccc ctgccctctc cgacagctca
7441 tgtttcgtca tgccccgctc atcgagcaga cgaaggatgt tggtgaaaaa tatctggttg
7501 tacacagcgg aagccgcccc tcgcaccttt ggtcgcggcc cgcaaaattt tagccgctaa
7561 agttcttgac agcggaacca atgtttagct aaactagagt ctcctttctc aaggagactt
7621 tcgatatgag ccataatcag ttccagttta tcggtaatct tacccgtgac accgaggtac
7681 gtcatggcaa ttctaacaag ccgcaagcaa ttttcgatat agcggttaat gaagagtggc
7741 gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct
7801 ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca
7861 agattcggaa taccaagtac gggaaggacg gccagacggt ctacgggacc gacttcattg
7921 ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc
7981 acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg
8041 accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa
8101 ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga
8161 tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc
8221 tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt
8281 tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg
8341 ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca
8401 cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca
8461 cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga
8521 aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga
8581 tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt
8641 tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt
8701 gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt
8761 cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg
8821 tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg
8881 tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat
8941 gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc
9001 accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca
9061 cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg
9121 gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg
9181 tggggtcagt tccggctggg ggttcagcag ccagcgcttt actggcattt caggaacaag
9241 cgggcactgc tcgacgcact tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta
9301 cgaactgccg ataaacagag gattaaaatt gacaattgtg attaaggctc agattcgacg
9361 gcttggagcg gccgacgtgc aggatttccg cgagatccga ttgtcggccc tgaagaaagc
9421 tccagagatg ttcgggtccg tttacgagca cgaggagaaa aagcccatgg aggcgttcgc
9481 tgaacggttg cgagatgccg tggcattcgg cgcctacatc gacggcgaga tcattgggct
9541 gtcggtcttc aaacaggagg acggccccaa ggacgctcac aaggcgcatc tgtccggcgt
9601 tttcgtggag cccgaacagc gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc
9661 ggcgggttta ttgctcgtga tgatcgtccg acagattcca acgggaatct ggtggatgcg
9721 catcttcatc ctcggcgcac ttaatatttc gctattctgg agcttgttgt ttatttcggt
9781 ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt
9841 gttcatctct gccgctctgc taggtagccc gatacgattg atggcggtcc tgggggctat
9901 ttgcggaact gcgggcgtgg cgctgttggt gttgacacca aacgcagcgc tagatcctgt
9961 cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg ttcggaaccg tgctgacccg
10021 caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc tggcaactgg cggccggagg
10081 acttctgctc gttccagtag ctttagtgtt tgatccgcca atcccgatgc ctacaggaac
10141 caatgttctc ggcctggcgt ggctcggcct gatcggagcg ggtttaacct atctagactg
10201 catgatggtg atagccgtca gcatgatttt cgtgtcgtcg aacttggtga agggcattct
10261 cggcggcgat cacgacgccg gccctgcgga gccttcgccg cgtgcgcgat tcatggcggc
10321 cgtggaggcc aaggatttcg cgcgagtgca agagctgatc gaggcgcgtg gagccaagtc
10381 ggcggctgat tatgtccttg cgcagctcgc cgtggccgaa ggtctggacc gcaagcctgg
10441 tgcgcgcgtc gtggtcggga aagcggcggg cagcatggca atgccgcctg cggcgctggg
10501 ttttacgcca aggggagaag cggcatacgc catcgagcgg tcagcctatg gtgagccgag
10561 gtccagcatt gcgaagcagt accagcagga atggaaccgg aaggcggcga cctggtgggc
10621 gatggccggt gtggccggca tcatcggcgc gatcctggcg gcggcggcaa ccggctttgt
10681 tgggctggca gtgtcgatcc gcaaccgagt gaagcgcgtg cgcgacctgt tggtgatgga
10741 gccgggtgca gagccataag cggcaagaga cgaaagcccg gtttccgggc ttttgttttg
10801 ttacgccaag gacgagtttt agcggctaaa ggtgttgacg tgcgagaaat gtttagctaa
10861 acttctctca tgtgctggcg gctgtcaccg ctatgttcaa ccaaggcgcg gagcaaatta
10921 tgggtgttat ccatgaagaa acggcttacc gaaagccagt tccaggaggc gatccagggg
10981 ctggaagtgg ggcagcagac catcgagata gcgcggggcg tcttagtcga tgggaagcca
11041 caggcgacgt tcgcaacgtc gctgggactg accaggggcg cagtgtcgca agcggtgcat
11101 cgcgtgtggg ccgcgttcga ggacaagaac ttgcccgagg ggtacgcgcg ggtaacggcg
11161 gttctgccgg aacatcaggc gtacatcgtc cggaagtggg aagcggacgc caagaaaaaa
11221 caggaaacca aacgatgaaa actttggtca cggccaacca gaaaggcggc gtcggcaaga
11281 cttcgaccct tgtgcatctt gccttcgact ttttcgagcg cggcttgcgg gttgccgtga
11341 tcgacctgga cccccagggc aatgcgtcct acacgctcaa ggactttgct accggcctgc
11401 atgcaagcaa gctgttcggc gctgtccctg ccggcggctg gaccgaaacc gcacccgcag
11461 ccggcgacgg ccaggccgcg cgcctcgccc tcatcgagtc caacccggta ctggcgaatg
11521 ccgaacggct gtcgctggac gacgcccgcg agctgttcgg ggcgaacatc aaggccctgg
11581 cgaaccaagg cttcgacgtg tgcctgatcg acacggcccc gacccttggc gtcggcctgg
11641 cggccgccct cttcgcggcc gactatgtgc tgtcccccat cgagcttgag gcgtacagca
11701 tccagggcat caagaagatg gtcacgacca ttgcgaacgt gcgccagaag aacgccaagc
11761 tgcaattcct tggcatggtg cccagcaagg tcgatgcgcg gaatccgcgc cacgcgcgcc
11821 accaagccga gctgctggcc gcgtacccca agatgatgat tccggccacc gttggcctgc
11881 gcagcagcat cgccgatgcc ctcgcatccg gtgtgccggt ctggaagatc aagaaaacgg
11941 ccgcgcgcaa ggcatcgaaa gaggttcgcg ccctggctga ttacgtgttc acgaagatgg
12001 agatttccca atgactgcgg ctcaagccaa gaccaccaag aaaaacaccg ctgcggccgc
12061 tcaggaagcc gcaggcgcgg cgcagccgtc cggcctgggg ttggatagca tcggcgacct
12121 gtcgagcctc ctggacgctc ctgcggcgtc tcagggcggt tccggcccta tcgagctgga
12181 cctggacctg atcgacgaag atccgcatca gccgcggacg gccgacaacc ccggcttttc
12241 cccggagagc atcgcggaaa tcggtgccac gatcaaagag cgcggggtga agtcacccat
12301 ttcggtgcgc gagaaccagg agcagccggg ccgctatatc atcaatcacg gcgcccgccg
12361 ctaccgtggc tcgaagtggg ccggcaagaa gtccatcccg gcgttcatcg acaacgacta
12421 caacgaagcc gaccaggtta tcgagaacct gcaacgcaac gagctgaccc cgcgcgaaat
12481 tgccgacttc attggccgcg agctggcgaa gggcaagaag aaaggcgata tcgccaagga
12541 aatcggcaag tcgccggcgt tcatcaccca gcacgtcacg ctgctggacc tgccggagaa
12601 gatcgccgat gcgttcaaca ccggccgcgt gcgcgacgtg accgtggtga acgagctggt
12661 gacggccttc aagaagcgcc cggaggaagt cgaggcgtgg cttgacgacg acacccagga
12721 aatcacgcgc ggcacggtca agctgctgcg cgagttcctg gacgagaagg gccgcgatcc
12781 caacaccgtc gatgccttca acggccagac tgatgccgag cgtgacgcgg aggccggcga
12841 cggccaggac ggcgaggacg gcgaccagga cggtaaggac gccaaggaaa agggcgcgaa
12901 ggagccggac ccggacaagc tgaaaaaggc catcgtccag gtcgagcacg acgagcgccc
12961 tgcccgcctt atcctcaacc gtcggccgcc ggcggaaggc tatgcctggt tgaagtacga
13021 ggacgacggc caggagttcg aggcgaacct tgccgacgtg aaactggtcg cgctcatcga
13081 gggctgatcc ccaaagacag cggcgcgggc cacccgcgcc gcacagacaa cggttccgct
13141 acaaggagga ccgaagaatg aatccgatgc tgttctacat cgcgggaggc gtaggcgcgg
13201 cgttgctgct ggtttccgcg atcatgctgt tcaagctgcg cgagccgaag aaggaacacc
13261 gaccgcagcg caaggcggcg gccccgacgc cgcagccggt cgataacgag ctgctgcgca
13321 cg
//
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