| 质粒名称: | pGBW-m4092677 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 153883 |
| 存储实验室: | Ginkgo Bioworks, Benjie Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pG9m-2 backbone |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 1968 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fragment SARS-CoV-2 orf1a stop to orf1b stop |
| 物种来源: | Severe acute respiratory syndrome coronavirus 2 |
| 插入大小: | 8071 bp |
| 基因突变: | wt;handful of SNPs as watermarks |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | Promoter | t7 consensus from NEB, G is +1 |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGBW-m4092677 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153883 ; http://n2t.net/addgene:153883 ; RRID:Addgene_153883) |
买家导航
pGBWm4092677载体基本信息
pGBWm4092677载体图谱质粒图谱和多克隆位点信息
pGBWm4092677载体简介
pGBWm4092677载体序列
LOCUS pGBW-m4092677 10107 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to
orf1b stop.
ACCESSION .
VERSION .
KEYWORDS pGBW-m4092677
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10107)
TITLE Cloned SARS-CoV-2 viral DNA fragments
REFERENCE 2 (bases 1 to 10107)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10107
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 7648..7695
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
terminator 7738..7769
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
promoter 7770..7872
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 7873..8733
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8091..8110)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS 8734..8925
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
terminator 8949..8976
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(8988..9575)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 9597..9626
/label=T3Te terminator
/note="phage T3 early transcription terminator"
promoter 9665..9683
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ttcaataaaa aggactggta tgattttgta gaaaacccag atatattacg cgtatacgcc
61 aacttaggtg aacgtgtacg ccaagctttg ttaaaaacag tacaattctg tgatgccatg
121 cgaaatgctg gtattgttgg tgtactgaca ttagataatc aagatctcaa tggtaactgg
181 tatgatttcg gtgatttcat acaaaccacg ccaggtagtg gagttcctgt tgtagattct
241 tattattcat tgttaatgcc tatattaacc ttgaccaggg ctttaactgc agagtcacat
301 gttgacactg acttaacaaa gccttacatt aagtgggatt tgttaaaata tgacttcacg
361 gaagagaggt taaaactctt tgaccgttat tttaaatatt gggatcagac ataccaccca
421 aattgtgtta actgtttgga tgacagatgc attctgcatt gtgcaaactt taatgtttta
481 ttctctacag tgttcccacc tacaagtttt ggaccactag tgagaaaaat atttgttgat
541 ggtgttccat ttgtagtttc aactggatac cacttcagag agctaggtgt tgtacataat
601 caggatgtaa acttacatag ctctagactt agttttaagg aattacttgt gtatgctgct
661 gaccctgcta tgcacgctgc ttctggtaat ctattactag ataaacgcac tacgtgcttt
721 tcagtagctg cacttactaa caatgttgct tttcaaactg tcaaacccgg taattttaac
781 aaagacttct atgactttgc tgtgtctaag ggtttcttta aggaaggaag ttctgttgaa
841 ttaaaacact tcttctttgc tcaggatggt aatgctgcta tcagcgatta tgactactat
901 cgttataatc taccaacaat gtgtgatatc agacaactac tatttgtagt tgaagttgtt
961 gataagtact ttgattgtta cgatggtggc tgtattaatg ctaaccaagt catcgtcaac
1021 aacctagaca aatcagctgg ttttccattt aataaatggg gtaaggctag actttattat
1081 gattcaatga gttatgagga tcaagatgca cttttcgcat atacaaaacg taatgtcatc
1141 cctactataa ctcaaatgaa tcttaagtat gccattagtg caaagaatag agctcgcacc
1201 gtagctggtg tctctatctg tagtactatg accaatagac agtttcatca aaaattattg
1261 aaatcaatag ccgccactag aggagctact gtagtaattg gaacaagcaa attctatggt
1321 ggttggcaca acatgttaaa aactgtttat agtgatgtag aaaaccctca ccttatgggt
1381 tgggattatc ctaaatgtga tagagccatg cctaacatgc ttagaattat ggcctcactt
1441 gttcttgctc gcaaacatac aacgtgttgt agcttgtcac accgtttcta tagattagct
1501 aatgagtgtg ctcaagtatt gagtgaaatg gtcatgtgtg gcggttcact atatgttaaa
1561 ccaggtggaa cctcatcagg agatgccaca actgcttatg ctaatagtgt ttttaacatt
1621 tgtcaagctg tcacggccaa tgttaatgca cttttatcta ctgatggtaa caaaattgcc
1681 gataagtatg tccgcaattt acaacacaga ctttatgagt gtctctatag aaatagagat
1741 gttgacacag actttgtgaa tgagttttac gcatatttgc gtaaacattt ctcaatgatg
1801 atactctctg acgatgctgt tgtgtgtttc aatagcactt atgcatctca aggtctagtg
1861 gctagcataa agaactttaa gtcagttctt tattatcaaa acaatgtttt tatgtctgaa
1921 gcaaaatgtt ggactgagac tgaccttact aaaggacctc atgaattttg ctctcaacat
1981 acaatgctag ttaaacaggg tgatgattat gtgtaccttc cttacccaga tccatcaaga
2041 atcctagggg ccggctgttt tgtagatgat atcgtaaaaa cagatggtac acttatgatt
2101 gaacggttcg tgtctttagc tatagatgct tacccactta ctaaacatcc taatcaggag
2161 tatgctgatg tctttcattt gtacttacaa tacataagaa agctacatga tgagttaaca
2221 ggacacatgt tagacatgta ttctgttatg cttactaatg ataacacttc aaggtattgg
2281 gaacctgagt tttatgaggc tatgtacaca ccgcatacag tcttacaggc tgttggggct
2341 tgtgttcttt gcaattcaca gacttcatta agatgtggtg cttgcatacg tagaccattc
2401 ttatgttgta aatgctgtta cgaccatgtc atatcaacat cacataaatt agtcttgtct
2461 gttaatccgt atgtttgcaa tgctccaggt tgtgatgtca cagatgtgac tcaactttac
2521 ttaggaggta tgagctatta ttgtaaatca cataaaccac ccattagttt tccattgtgt
2581 gctaatggac aagtttttgg tttatataaa aatacatgtg ttggtagcga taatgttact
2641 gactttaatg caattgcaac atgtgactgg acaaatgctg gtgattacat tttagctaac
2701 acctgtactg aaagactcaa gctttttgca gcagaaacgc tcaaagctac tgaggagaca
2761 tttaaactgt cttatggtat tgctactgta cgtgaagtgc tgtctgacag agaattacat
2821 ctttcatggg aagttggtaa acctagacca ccacttaacc gaaattatgt ctttactggt
2881 tatcgtgtaa ctaaaaacag taaagtacaa ataggagagt acacctttga aaaaggtgac
2941 tatggtgatg ctgttgttta ccgaggtaca acaacttaca aattaaatgt tggtgattat
3001 tttgtgctga catcacatac agtaatgcca ttaagtgcac ctacactagt gccacaagag
3061 cactatgtta gaattactgg cttataccca acactcaata tctcagatga gttttctagc
3121 aatgttgcaa attatcaaaa ggttggtatg caaaagtatt ctacactcca gggaccacct
3181 ggtactggta agagtcattt tgctattggc ctagctctct actacccttc tgctcgcata
3241 gtgtatacag cttgctctca tgccgctgtt gatgcactat gtgagaaggc attaaaatat
3301 ttgcctatag ataaatgtag tagaattata cctgcacgtg ctcgtgtaga gtgttttgat
3361 aaattcaaag tgaattcaac attagaacag tatgtctttt gtactgtaaa tgcattgcct
3421 gagacgacag cagatatagt tgtctttgat gaaatttcaa tggccacaaa ttatgatttg
3481 agtgttgtca atgccagatt acgtgctaag cactatgtgt acattggcga ccctgctcaa
3541 ttacctgcac cacgcacatt gctaactaag ggcacactag aaccagaata tttcaattca
3601 gtgtgtagac ttatgaaaac tataggtcca gacatgttcc tcggaacttg tcggcgttgt
3661 cctgctgaaa ttgttgacac tgtgagtgct ttggtttatg ataataagct taaagcacat
3721 aaagacaaat cagctcaatg ctttaaaatg ttttataagg gtgttatcac gcatgatgtt
3781 tcatctgcaa ttaacaggcc acaaataggc gtggtaagag aattccttac acgtaaccct
3841 gcttggagaa aagctgtctt tatttcacct tataattcac agaatgctgt agcctcaaag
3901 attttgggac taccaactca aactgttgat tcatcacagg gctcagaata tgactatgtc
3961 atattcactc aaaccactga aacagctcac tcttgtaatg taaacagatt taatgttgct
4021 attaccagag caaaagtagg catactttgc ataatgtctg atagagacct ttatgacaag
4081 ttgcaattta caagtcttga aattccacgt aggaatgtgg caactttaca agctgaaaat
4141 gtaacaggac tctttaaaga ttgtagtaag gtaatcactg ggttacatcc tacacaggca
4201 cctacacacc tcagtgttga cactaaattc aaaactgaag gtttatgtgt tgacatacct
4261 ggcataccta aggacatgac ctatagaaga ctcatctcta tgatgggttt taaaatgaat
4321 tatcaagtta atggttaccc taacatgttt atcacccgcg aagaagctat aagacatgta
4381 cgtgcatgga ttggcttcga tgtcgagggg tgtcatgcta ctagagaagc tgttggtacc
4441 aatttacctt tacagctagg tttttctaca ggtgttaacc tagttgctgt acctacaggt
4501 tatgttgata cacctaataa tacagatttt tccagagtta gtgctaaacc accgcctgga
4561 gatcaattta aacacctcat accacttatg tacaaaggac ttccttggaa tgtagtgcgt
4621 ataaagattg tacaaatgtt aagtgacaca cttaaaaatc tctctgacag agtcgtattt
4681 gtcttatggg cacatggctt tgagttgaca tctatgaagt attttgtgaa aataggacct
4741 gagcgcacct gttgtctatg tgatagacgt gccacatgct tttccactgc ttcagacact
4801 tatgcctgtt ggcatcattc tattggattt gattacgtct ataatccgtt tatgattgat
4861 gttcaacaat ggggttttac aggtaaccta caaagcaacc atgatctgta ttgtcaagtc
4921 catggtaatg cacatgtagc tagttgtgat gcaatcatga ctaggtgtct agctgtccac
4981 gagtgctttg ttaagcgtgt tgactggact attgaatatc ctataattgg tgatgaactg
5041 aagattaatg cggcttgtag aaaggttcaa cacatggttg ttaaagctgc attattagca
5101 gacaaattcc cagttcttca cgacattggt aaccctaaag ctattaagtg tgtacctcaa
5161 gctgatgtag aatggaagtt ctatgatgca cagccttgta gtgacaaagc ttataaaata
5221 gaagaattat tctattctta tgccacacat tctgacaaat tcacagatgg tgtatgccta
5281 ttttggaatt gcaatgtcga tagatatcct gctaattcca ttgtttgtag atttgacact
5341 agagtgctat ctaaccttaa cttgcctggt tgtgatggtg gcagtttgta tgtaaataaa
5401 catgcattcc acacaccagc ttttgataaa agtgcttttg ttaatttaaa acaattacca
5461 tttttctatt actctgacag tccatgtgag tctcatggaa aacaagtagt gtcagatata
5521 gattatgtac cactaaagtc tgctacgtgt ataacacgtt gcaatttagg tggtgctgtc
5581 tgtagacatc atgctaatga gtacagattg tatctcgatg cttataacat gatgatctca
5641 gctggcttta gcttgtgggt ttacaaacaa tttgatactt ataacctctg gaacactttt
5701 acaagacttc agagtttaga aaatgtggct tttaatgttg taaataaggg acactttgat
5761 ggacaacagg gtgaagtacc agtttctatc attaataaca ctgtttacac aaaagttgat
5821 ggtgttgatg tagaattgtt tgaaaataaa acaacattac ctgttaatgt agcatttgag
5881 ctttgggcta agcgcaacat taaaccagta ccagaggtga aaatactcaa taatttgggt
5941 gtggacattg ctgctaatac tgtgatctgg gactacaaaa gagatgctcc agcacatata
6001 tctactattg gtgtttgttc tatgactgac atagccaaga aaccaactga aacgatttgt
6061 gcaccactca ctgtcttttt tgatggtaga gttgatggtc aagtagactt atttagaaat
6121 gcccgtaatg gtgttcttat tacagaaggt agtgttaaag gtttacaacc atctgtaggt
6181 cccaaacaag ctagtcttaa tggagtcaca ttaattggag aagccgtaaa aacacagttc
6241 aattattata agaaagttga tggtgttgtc caacaattac ctgaaactta ctttactcag
6301 agtagaaatt tacaagaatt taaacccagg agtcaaatgg aaattgattt cttagaatta
6361 gctatggatg aattcattga acggtataaa ttagaaggct atgccttcga acatatcgtt
6421 tatggagatt ttagtcatag tcagttaggt ggtttacatc tactgattgg actagctaaa
6481 cgttttaagg aatcaccttt tgaattagaa gattttattc ctatggacag tacagttaaa
6541 aactatttca taacagatgc gcaaacaggt tcatctaagt gtgtgtgttc tgttattgat
6601 ttattacttg atgattttgt tgaaataata aaatcccaag atttatctgt agtttctaag
6661 gttgtcaaag tgactattga ctatacagaa atttcattta tgctttggtg taaagatggc
6721 catgtagaaa cattttaccc aaaattacaa tctagtcaag cgtggcaacc gggtgttgct
6781 atgcctaatc tttacaaaat gcaaagaatg ctattagaaa agtgtgacct tcaaaattat
6841 ggtgatagtg caacattacc taaaggcata atgatgaatg tcgcaaaata tactcaactg
6901 tgtcaatatt taaacacatt aacattagct gtaccctata atatgagagt tatacatttt
6961 ggtgctggtt ctgataaagg agttgcacca ggtacagctg ttttaagaca gtggttgcct
7021 acgggtacgc tgcttgtcga ttcagatctt aatgactttg tctctgatgc agattcaact
7081 ttgattggtg attgtgcaac tgtacataca gctaataaat gggatctcat tattagtgat
7141 atgtacgacc ctaagactaa aaatgttaca aaagaaaatg actctaaaga gggttttttc
7201 acttacattt gtgggtttat acaacaaaag ctagctcttg gaggttccgt ggctataaag
7261 ataacagaac attcttggaa tgctgatctt tataagctca tgggacactt cgcatggtgg
7321 acagcctttg ttactaatgt gaatgcgtca tcatctgaag catttttaat tggatgtaat
7381 tatcttggca aaccacgcga acaaatagat ggttatgtca tgcatgcaaa ttacatattt
7441 tggaggaata caaatccaat tcagttgtct tcctattctt tatttgacat gagtaaattt
7501 ccccttaaat taaggggtac tgctgttatg tctttaaaag aaggtcaaat caatgatatg
7561 attttatctc ttcttagtaa aggtagactt ataattagag aaaacaacag agttgttatt
7621 tctagtgatg ttcttgttaa caactaacta gcataacccc ttggggcctc taaacgggtc
7681 ttgaggggtt ttttgcatca ctgaccattt aaatcatacc tgacctccat agcagaaagt
7741 caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac
7801 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
7861 aaggaagcta aaatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
7921 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
7981 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
8041 agtttacgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
8101 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
8161 cagaatgact tggttgagta ctcaccagtc acagaaaagc atctcacgga tggcatgaca
8221 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
8281 ctggcaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
8341 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
8401 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
8461 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
8521 tcacttctgc gctcggccct cccggctggc tggtttattg ctgataaatc tggagccggt
8581 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgcatc
8641 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
8701 gagataggtg cctcactgat taagcattgg taagtgacca aacaggaaaa aaccgccctt
8761 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
8821 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
8881 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgatgagg gcccaaatgt
8941 aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc
9001 cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca
9061 ggactataaa gataccaggc gtttacccct ggaagctccc tcgtgcgctc tcctgttccg
9121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
9181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
9241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
9301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
9361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
9421 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag
9481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
9541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa
9601 gaaaggccca cccgtgaagg tgagccagtg agttgattgc agtccagtta cgctggagtc
9661 aagctaatac gactcactat aggtgcagcc cgtcttacac cgtgcggcac aggcactagt
9721 actgatgtcg tatacagggc ttttgacatc tacaatgata aagtagctgg ttttgctaaa
9781 ttcctaaaaa ctaattgttg tcgcttccaa gaaaaggacg aagatgacaa tttaattgat
9841 tcttactttg tagttaagag acacactttc tctaactacc aacatgaaga aacaatttat
9901 aatttactta aggattgtcc agctgttgct aaacatgact tctttaagtt tagaatagac
9961 ggtgacatgg taccacatat atcacgtcaa cgtcttacta aatacacaat ggcagacctc
10021 gtctatgctt taaggcattt tgatgaaggt aattgtgaca cattaaaaga aatacttgtc
10081 acatacaatt gttgtgatga tgattat
//
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