| 质粒名称: | pTK095 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 59560 |
| 存储实验室: | Ron Weiss |
|
载体骨架基本信息 |
|
| 载体名称: | SIN |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTK095 was a gift from Ron Weiss (Addgene plasmid # 59560 ; http://n2t.net/addgene:59560 ; RRID:Addgene_59560) |
| 参考文献部分: | Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, Weiss R. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301. 10.1038/nbt.3301 PubMed 26237515 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTK095 11412 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2.
ACCESSION .
VERSION .
KEYWORDS pTK095
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11412)
AUTHORS Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H,
Weiss R
TITLE Mammalian synthetic circuits with RNA binding proteins for RNA-only
delivery.
JOURNAL Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
PUBMED 26237515
REFERENCE 2 (bases 1 to 11412)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11412
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 7984..8040
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 8041..8760
/codon_start=1
/product="enhanced YFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEYFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(8086..8107)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(8347..8366)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 8694..8715
/label=EGFP-C
/note="EGFP, forward primer"
misc_RNA 8901..8919
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 8940..8958
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 8980..8998
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 9019..9037
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 9075..9093
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 9114..9132
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 9154..9172
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 9193..9211
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
primer_bind complement(9497..9515)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 9583..9687
/gene="bla"
/label=AmpR promoter
CDS 9688..10548
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9906..9925)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10719..11307
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11208..11227
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 11395..1
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 11395..11412
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta
361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca
3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca
3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
3841 tgaattgcct taactcagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
4621 atgagttagt atggatccat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tgccaccatg
7621 tacgtgagat ttgaggttcc tgaggacatg cagaacgaag ctctgagtct gctggagaag
7681 gttagggaga gcggtaaggt aaagaaaggt accaacgaga cgacaaaggc tgtggagagg
7741 ggactggcaa agctcgttta catcgcagag gatgttgacc cgcctgagat cgttgctcat
7801 ctgcccctcc tctgcgagga gaagaatgtg ccgtacattt acgttaaaag caagaacgac
7861 cttggaaggg ctgtgggcat tgaggtgcca tgcgcttcgg cagcgataat caacgaggga
7921 gagctgagaa aggagcttgg aagccttgtg gagaagatta aaggccttca gaagggatct
7981 ggcgccacca acttctctct gctgaagcag gccggcgacg tggaggagaa cccaggccca
8041 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
8101 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
8161 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
8221 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
8281 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
8341 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
8401 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
8461 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
8521 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
8581 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
8641 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
8701 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
8761 ggcgcgcccc gcttgaagtc tttaattaaa ccgcttgaag tctttaatta aaccgcttga
8821 agtctttaat taaaccgctt gaagtcttta attaaaacta gttaaagaac gagcagtaat
8881 tctaggcgat cgctcgaaaa acatgaggat cacccatgtc tgcaggtcga ctctagaaaa
8941 catgaggatc acccatgtcc tgcaggtcga ctctagaaaa catgaggatc acccatgtct
9001 gcaggtcgac tctagaaaac atgaggatca cccatgtcct cgagtacaag taattctagg
9061 cgatcgctcg aaaaacatga ggatcaccca tgtctgcagg tcgactctag aaaacatgag
9121 gatcacccat gtcctgcagg tcgactctag aaaacatgag gatcacccat gtctgcaggt
9181 cgactctaga aaacatgagg atcacccatg tcctcgaggt gtgcggccgc tggcctcgag
9241 gaagcgcagt acataatgct gcgcagtgtt gccacataac cactatatta accatttatc
9301 tagcggacgc caaaaactca atgtatttct gaggaagcgt ggtgcataat gccacgcagc
9361 gtctgcataa cttttattat ttcttttatt aatcaacaaa attttgtttt taacatttca
9421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaagggga attcgagctc gggaattaat
9481 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
9541 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
9601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
9661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
9721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
9781 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
9841 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
9901 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
9961 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
10021 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
10081 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
10141 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
10201 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
10261 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
10321 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
10381 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
10441 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
10501 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
10561 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
10621 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
10681 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
10741 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
10801 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
10861 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
10921 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
10981 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
11041 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
11101 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
11161 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
11221 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
11281 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cgagctcgta tggacatatt
11341 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
11401 gtgacactat ag
//
