| 质粒名称: | SaABE8e |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 138500 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV with a BR322 origin |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 7275bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | iRFP713 |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 951 bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | JN247409 |
| 启动子: | CMV |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | |
买家导航
SaABE8e载体质粒基本信息
SaABE8e质粒图谱载体图谱和SaABE8e载体序列质粒序列多克隆位点信息
SaABE8e质粒载体简介
SaABE8e质粒序列载体序列
LOCUS SaABE8e 7275 bp DNA circular SYN 25-AUG-2024
DEFINITION A-to-G base editor.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7275)
AUTHORS Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW,
Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR
TITLE Phage-assisted evolution of an adenine base editor with improved Cas
domain compatibility and activity.
JOURNAL Nat Biotechnol. 2020 Jul;38(7):883-891. doi:
10.1038/s41587-020-0453-z. Epub 2020 Mar 16.
PUBMED 32433547
REFERENCE 2 (bases 1 to 7275)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41587-020-0453-z"; journalName: "Nat Biotechnol"; date:
"2020-07"; volume: "38"; issue: "7"; pages: "883-891"
FEATURES Location/Qualifiers
source 1..7275
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 445..465
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4261..4281
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4290..4307
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(4330..4347)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4336..4560
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(4631..4647)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4631..4647)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4644..4666)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4655..4671
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4679..4709)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4724..4745
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4862..4879)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5033..5621)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5113..5132)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5792..6652)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6415..6434
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6653..6757)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6836..6855)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer join(7027..7275,1..131)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg ggatgagagg
541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
781 aggaactcaa aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
901 gatttctatc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
961 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag ggaagcgaaa ttacattctg
1081 gggctggcca ttggcattac atcagtgggc tatggcatca ttgactacga gacaagggac
1141 gtgatcgacg ccggcgtgag actgttcaag gaggccaacg tggagaacaa tgagggccgg
1201 agatccaaga ggggagcaag gcgcctgaag cggagaaggc gccacagaat ccagagagtg
1261 aagaagctgc tgttcgatta caacctgctg accgaccact ccgagctgtc tggcatcaat
1321 ccttatgagg ccagagtgaa gggcctgtcc cagaagctgt ctgaggagga gtttagcgcc
1381 gccctgctgc acctggcaaa gaggagaggc gtgcacaacg tgaatgaggt ggaggaggac
1441 accggcaacg agctgtccac aaaggagcag atcagccgca attccaaggc cctggaggag
1501 aagtatgtgg ccgagctgca gctggagcgg ctgaagaagg atggcgaggt gaggggctcc
1561 atcaatcgct tcaagacctc tgactacgtg aaggaggcca agcagctgct gaaggtgcag
1621 aaggcctacc accagctgga tcagtccttt atcgatacat atatcgacct gctggagaca
1681 aggcgcacat actatgaggg accaggagag ggctctccct tcggctggaa ggacatcaag
1741 gagtggtacg agatgctgat gggccactgc acctattttc cagaggagct gagaagcgtg
1801 aagtacgcct ataacgccga tctgtacaac gccctgaatg acctgaacaa cctggtcatc
1861 accagggatg agaacgagaa gctggagtac tatgagaagt tccagatcat cgagaacgtg
1921 ttcaagcaga agaagaagcc tacactgaag cagatcgcca aggagatcct ggtgaacgag
1981 gaggacatca agggctaccg cgtgacctcc acaggcaagc cagagttcac caatctgaag
2041 gtgtatcacg atatcaagga catcacagcc cggaaggaga tcatcgagaa cgccgagctg
2101 ctggatcaga tcgccaagat cctgaccatc tatcagagct ccgaggacat ccaggaggag
2161 ctgaccaacc tgaatagcga gctgacacag gaggagatcg agcagatcag caatctgaag
2221 ggctacaccg gcacacacaa cctgagcctg aaggccatca atctgatcct ggatgagctg
2281 tggcacacaa acgacaatca gatcgccatc tttaaccggc tgaagctggt gccaaagaag
2341 gtggacctgt cccagcagaa ggagatccca accacactgg tggacgattt catcctgtct
2401 cccgtggtga agcggagctt catccagagc atcaaagtga tcaacgccat catcaagaag
2461 tacggcctgc ccaatgatat catcatcgag ctggccaggg agaagaactc caaggacgcc
2521 cagaagatga tcaatgagat gcagaagagg aaccgccaga ccaatgagcg gatcgaggag
2581 atcatcagaa ccacaggcaa ggagaacgcc aagtacctga tcgagaagat caagctgcac
2641 gatatgcagg agggcaagtg tctgtattct ctggaggcca tccctctgga ggacctgctg
2701 aacaatccat tcaactacga ggtggatcac atcatccccc ggagcgtgag cttcgacaat
2761 tcttttaaca ataaggtgct ggtgaagcag gaggagaaca gcaagaaggg caataggacc
2821 cctttccagt acctgtctag ctccgattct aagatcagct acgagacatt caagaagcac
2881 atcctgaatc tggccaaggg caagggccgc atcagcaaga ccaagaagga gtacctgctg
2941 gaggagcggg acatcaacag attctccgtg cagaaggact tcatcaaccg gaatctggtg
3001 gacaccagat acgccacacg cggcctgatg aatctgctgc ggtcttattt cagagtgaac
3061 aatctggatg tgaaggtgaa gagcatcaac ggcggcttca cctcctttct gcggagaaag
3121 tggaagttta agaaggagcg caacaagggc tataagcacc acgccgagga tgccctgatc
3181 atcgccaatg ccgacttcat ctttaaggag tggaagaagc tggacaaggc caagaaagtg
3241 atggagaacc agatgttcga ggagaagcag gccgagagca tgcccgagat cgagacagag
3301 caggagtaca aggagatttt catcacacct caccagatca agcacatcaa ggacttcaag
3361 gactacaagt attctcacag ggtggataag aagcccaacc gcgagctgat caatgacacc
3421 ctgtatagca cacggaagga cgataagggc aataccctga tcgtgaacaa tctgaacggc
3481 ctgtacgaca aggataatga caagctgaag aagctgatca acaagtctcc cgagaagctg
3541 ctgatgtacc accacgatcc tcagacatat cagaagctga agctgatcat ggagcagtac
3601 ggcgacgaga agaacccact gtataagtac tatgaggaga caggcaacta cctgacaaag
3661 tatagcaaga aggataatgg ccccgtgatc aagaagatca agtactatgg caacaagctg
3721 aatgcccacc tggacatcac cgacgattac cctaactctc gcaataaggt ggtgaagctg
3781 agcctgaagc cataccggtt cgacgtgtac ctggacaacg gcgtgtataa gtttgtgaca
3841 gtgaagaatc tggatgtgat caagaaggag aactactatg aggtgaacag caagtgctac
3901 gaggaggcca agaagctgaa gaagatcagc aaccaggccg agttcatcgc ctctttttac
3961 aacaatgacc tgatcaagat caatggcgag ctgtatagag tgatcggcgt gaacaatgat
4021 ctgctgaaca gaatcgaagt gaatatgatc gacatcacct acagggagta tctggagaac
4081 atgaatgata agaggccccc tcgcatcatc aagaccatcg cctctaagac acagagcatc
4141 aagaagtaca gcacagacat cctggggaac ctgtatgaag tcaagagcaa gaaacatcct
4201 cagattatca agaaaggctc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
4261 cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc
4321 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
4381 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
4441 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
4501 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
4561 cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg
4621 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
4681 acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca
4741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
4801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
4861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
4921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
4981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
5041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
5101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
5161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
5221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
5281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
5341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
5401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
5461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
5521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
5581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
5641 ctacggggtc tgacactcag tggaacgaaa actcacgtta agggattttg gtcatgagat
5701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
5761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
5821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
5881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
5941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
6001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
6061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
6121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
6181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
6241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
6301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
6361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
6421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
6481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
6541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
6601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
6661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
6721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
6781 ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa
6841 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
6901 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
6961 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
7021 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
7081 atagcccata tattgagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
7141 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
7201 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
7261 tacatcaagt gtatc
//
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