pSV2-DHFR

价格:1350元

联系方式:I47-825O-882O

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pSV2DHFR载体基本信息

出品公司: ATCC
载体名称: pSV2DHFR, pSV2-DHFR, ATCC37146, ATCC 37146
质粒类型: 哺乳动物细胞表达载体
启动子: SV40
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4900 bp
5' 测序引物:   SV40pro-F: TATTTATGCAGAGGCCGAGG
3' 测序引物: SV40pA-R: GAAATTTGTGATGCTATTGC
载体标签: C-DHFR ( mouse DHFR)
载体抗性: 氨苄
筛选标记: MTX (甲氨蝶呤)
备注:
pSV2-DHFR载体含有的是小鼠的DHFR基因,同时该载体是哺乳动物细胞和大肠杆菌穿梭质粒,在DHFR细胞系中,可以通过甲氨蝶呤对转染的细胞进行筛选。
产品目录号: 37146
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 非病毒

pSV2DHFR载体质粒图谱和多克隆位点信息

pSV2-DHFR载体图谱
pSV2-DHFR载体图谱

pSV2DHFR载体简介

pSV2DHFR载体序列

LOCUS       pSV2-DHFR	4900 bp 	DNA   circular 	SYN
DEFINITION  pSV2-DHFR
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="pSV2-dhfr"
                     /mol_type="other DNA"
     misc_feature    complement(5..25)
                     /label="pBABE_3_primer"
     misc_feature    complement(11..226)
                     /label="SV40_enhancer"
     promoter        23..291
                     /label="SV40_promoter"
     rep_origin      190..267
                     /label="SV40_origin"
     misc_feature    252..271
                     /label="SV40pro_F_primer"
     CDS             362..925
                     /label="mouse DHFR"
     misc_feature    1081..1096
                     /label="SV40_int"
     misc_feature    1102..1149
                     /label="SV40_3_splice"
     CDS             1226..1699
                     /label="ORF frame 2"
     misc_feature    1277..1297
                     /label="SV40 NLS"
     misc_feature    1283..1297
                     /label="NLS"
     terminator      1725..1856
                     /label="SV40_PA_terminator"
     CDS             complement(1797..2558)
                     /label="ORF frame 3"
     misc_feature    1813..1832
                     /label="EBV_rev_primer"
     promoter        2744..2772
                     /label="AmpR_promoter"
     gene            2814..3674
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2814..3674
                     /label="ORF frame 3"
     rep_origin      3829..4448
                     /label="pBR322_origin"
     misc_feature    4845..4867
                     /label="pGEX_3_primer"
ORIGIN
    1 cTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
   61 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA
  121 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
  181 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
  241 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
  301 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTATCC CCGCTGCCAT
  361 CATGGTTCGA CCATTGAACT GCATCGTCGC CGTGTCCCAA GATATGGGGA TTGGCAAGAA
  421 CGGAGACCTA CCCTGGCCTC CGCTCAGGAA CGAGTTCAAG TACTTCCAAA GAATGACCAC
  481 AACCTCTTCA GTGGAAGGTA AACAGAATCT GGTGATTATG GGTAGGAAAA CCTGGTTCTC
  541 CATTCCTGAG AAGAATCGAC CTTTAAAGGA CAGAATTAAT ATAGTTCTCA GTAGAGAACT
  601 CAAAGAACCA CCACGAGGAG CTCATTTTCT TGCCAAAAGT TTGGATGATG CCTTAAGACT
  661 TATTGAACAA CCGGAATTGG CAAGTAAAGT AGACATGGTT TGGATAGTCG GAGGCAGTTC
  721 TGTTTACCAG GAAGCCATGA ATCAACCAGG CCACCTCAGA CTCTTTGTGA CAAGGATCAT
  781 GCAGGAATTT GAAAGTGACA CGTTTTTCCC AGAAATTGAT TTGGGGAAAT ATAAACTTCT
  841 CCCAGAATAC CCAGGCGTCC TCTCTGAGGT CCAGGAGGAA AAAGGCATCA AGTATAAGTT
  901 TGAAGTCTAC GAGAAGAAAG ACTAACAGGA AGATGCTTTC AAGTTCTCTG CTCCCCTCCT
  961 AAAGCTATGC ATTTTTATAA GACCATGGGA CTTTTGCTGG CTTTAGATCT TTGTGAAGGA
 1021 ACCTTACTTC TGTGGTGTGA CATAATTGGA CAAACTACCT ACAGAGATTT AAAGCTCTAA
 1081 GGTAAATATA AAATTTTTAA GTGTATAATG TGTTAAACTA CTGATTCTAA TTGTTTGTGT
 1141 ATTTTAGATT CCAACCTATG GAACTGATGA ATGGGAGCAG TGGTGGAATG CCTTTAATGA
 1201 GGAAAACCTG TTTTGCTCAG AAGAAATGCC ATCTAGTGAT GATGAGGCTA CTGCTGACTC
 1261 TCAACATTCT ACTCCTCCAA AAAAGAAGAG AAAGGTAGAA GACCCCAAGG ACTTTCCTTC
 1321 AGAATTGCTA AGTTTTTTGA GTCATGCTGT GTTTAGTAAT AGAACTCTTG CTTGCTTTGC
 1381 TATTTACACC ACAAAGGAAA AAGCTGCACT GCTATACAAG AAAATTATGG AAAAATATTC
 1441 TGTAACCTTT ATAAGTAGGC ATAACAGTTA TAATCATAAC ATACTGTTTT TTCTTACTCC
 1501 ACACAGGCAT AGAGTGTCTG CTATTAATAA CTATGCTCAA AAATTGTGTA CCTTTAGCTT
 1561 TTTAATTTGT AAAGGGGTTA ATAAGGAATA TTTGATGTAT AGTGCCTTGA CTAGAGATCA
 1621 TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC
 1681 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT
 1741 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
 1801 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCCCA
 1861 GGAAGCTCCT CTGTGTCCTC ATAAACCCTA ACCTCCTCTA CTTGAGAGGA CATTCCAATC
 1921 ATAGGCTGCC CATCCACCCT CTGTGTCCTC CTGTTAATTA GGTCACTTAA CAAAAAGGAA
 1981 ATTGGGTAGG GGTTTTTCAC AGACCGCTTT CTAAGGGTAA TTTTAAAATA TCTGGGAAGT
 2041 CCCTTCCACT GCTGTGTTCC AGAAGTGTTG GTAAACAGCC CACAAATGTC AACAGCAGAA
 2101 ACATACAAGC TGTCAGCTTT GCACAAGGGC CCAACACCCT GCTCATCAAG AAGCACTGTG
 2161 GTTGCTGTGT TAGTAATGTG CAAAACAGGA GGCACATTTT CCCCACCTGT GTAGGTTCCA
 2221 AAATATCTAG TGTTTTCATT TTTACTTGGA TCAGGAACCC AGCACTCCAC TGGATAAGCA
 2281 TTATCCTTAT CCAAAACAGC CTTGTGGTCA GTGTTCATCT GCTGACTGTC AACTGTAGCA
 2341 TTTTTTGGGG TTACAGTTTG AGCAGGATAT TTGGTCCTGT AGTTTGCTAA CACACCCTGC
 2401 AGCTCCAAAG GTTCCCCACC AACAGCAAAA AAATGAAAAT TTGACCCTTG AATGGGTTTT
 2461 CCAGCACCAT TTTCATGAGT TTTTTGTGTC CCTGAATGCA AGTTTAACAT AGCAGTTACC
 2521 CCAATAACCT CAGTTTTAAC AGTAACAGCT TCCCACATCA AAATATTTCC ACAGGTTAAG
 2581 TCCTCATTTA AATTAGGCAA AGGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT
 2641 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG
 2701 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
 2761 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
 2821 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
 2881 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG
 2941 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC
 3001 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG
 3061 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT
 3121 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG
 3181 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC
 3241 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT
 3301 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG
 3361 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC
 3421 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC
 3481 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA
 3541 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG
 3601 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA
 3661 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC
 3721 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA
 3781 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT
 3841 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
 3901 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
 3961 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC
 4021 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
 4081 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
 4141 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
 4201 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
 4261 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
 4321 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
 4381 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
 4441 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC
 4501 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG
 4561 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC
 4621 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC
 4681 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG
 4741 TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC
 4801 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG
 4861 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG
//

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