| 质粒名称: | pCytERM-StayGold-N1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000262 |
| 产品用途: | Expresses CytERM-StayGold in mammalian cell culture |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | CytERM-StayGold |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
|
pCytERM-StayGold-N1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCytERM-StayGold-N1载体质粒基本信息
pCytERM-StayGold-N1质粒图谱载体图谱和pCytERM-StayGold-N1载体序列质粒序列多克隆位点信息
pCytERM-StayGold-N1质粒载体简介
pCytERM-StayGold-N1质粒序列载体序列
LOCUS pCytERM-StayGold-N1 4740 bp DNA circular SYN 01-NOV-2023
DEFINITION Vector for fusing EGFP to the C-terminus of a partner protein. For
other reading frames, use pEGFP-N2 or pEGFP-N3.
ACCESSION U55762
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4740)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Jul 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4740
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 615..701
/codon_start=1
/product="cytochrome P-450 IIC1, partial [Oryctolagus
cuniculus]
Sequence ID: AAA31436.1Length: 56Number of Matches: 1"
/label=cytochrome P-450
/translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
CDS 753..1403
/codon_start=1
/label=StayGold
/translation="MASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKL
PMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFM
KNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVQHIPRDDGVECPVTLLYPLLSDKS
KCVEAHQNTICKPLHNQPAPDVPYHWIRKQYTQSKDDTEERDHICQSETLEAHL"
primer_bind complement(1377..1429)
/label=0708-CytERM-4567-R
polyA_signal 1528..1649
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1656..2111)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2138..2242
/gene="bla"
/label=AmpR promoter
promoter 2244..2601
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2452..2587
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2636..3430
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3662..3709
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4038..4626
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctatggac cctgtggtgg tgctggggct ctgtctctcc tgtttgcttc
661 tcctttcact ctggaaacag agctatgggg gagggaagct tcgaattctg cagtcgacgg
721 taccgcgggc ccgggatcca ccggtcgcca ccatggctag tacaccattt aaatttcaac
781 ttaaaggaac catcaatggc aaatcgttta ccgttgaagg cgaaggtgaa ggaaattcac
841 atgaaggttc tcataaagga aaatatgttt gtacaagtgg aaaactaccg atgtcatggg
901 cagcacttgg aacatccttt ggttatggaa tgaaatatta taccaaatat cctagtggac
961 tgaagaactg gtttcatgaa gtaatgcctg aaggctttac ctacgatcgt catattcaat
1021 ataaaggcga tgggagtatc catgcaaaac accaacactt tatgaaaaat gggacttatc
1081 acaacattgt tgaatttact ggtcaggatt ttaaagaaaa tagtccagtc ttaactggag
1141 atatgaatgt ctcattaccg aatgaagttc aacatatacc cagagatgat ggagtagaat
1201 gcccagtgac cttgctttat cctttattat cggataaatc aaaatgcgtt gaggctcacc
1261 aaaatacaat ctgcaagcct cttcataatc aaccagcacc tgatgtccca tatcactgga
1321 ttcgtaaaca atacacacaa agcaaagatg ataccgagga acgtgatcat atttgtcaat
1381 cagagactct cgaagcacac ttataagcgg ccgcgactct agatcataat cagccatacc
1441 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
1501 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
1561 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
1621 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
1681 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
1741 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
1801 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
1861 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
1921 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
1981 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
2041 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
2101 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
2161 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
2221 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
2281 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2341 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2401 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2461 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2521 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
2581 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
2641 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
2701 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
2761 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
2821 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
2881 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
2941 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
3001 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
3061 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
3121 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
3181 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
3241 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
3301 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
3361 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
3421 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
3481 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
3541 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
3601 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
3661 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
3721 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
3781 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
3841 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
3901 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
3961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
4021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
4081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
4141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
4201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
4261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
4321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
4381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
4441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
4501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
4561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
4621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
4681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
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