pDisplay-NE2m-IRES-mCherry-CAAX

LOCUS       pDisplay-NE2m-IRES-mCherry-CAAX               8543 bp DNA     circular SYN 23-JAN-2024
DEFINITION  Expresses the genetically-encoded fluorescent norepinephrine (NE) 
            sensor GRAB_NE2m and a membrane-localized mcherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDisplay-NE2m-IRES-mCherry-CAAX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  TITLE     Monitoring norepinephrine release in vivo using next-generation 
            GRABNE sensors
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             737..2734
                     /codon_start=1
                     /label=NE2m
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGGSLQPDAGNASWN
                     GTEAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLV
                     SLASADILVATLVIPFSLANEVMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRY
                     WSITQAKEYNLKRTPRRIKAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEIN
                     DQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRP
                     NGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEEGGNVYIKADKQKNG
                     IKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVSGEG
                     EGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGY
                     IQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARW
                     RGRQNREKRFFFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPRTLFKFFFWFGYCNSSL
                     NPVIYTIFNHDFRRAFKKILCRGDRKRIVL"
     sig_peptide     737..799
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1927..1948
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2054..2075)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2315..2334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2742..3327
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2909..2926)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3135..3154
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3328..4035
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3473..3491)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3741..3760
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4093..4122
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4126..4272
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4294..4311)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4300..4524
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4656..5244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4736..4755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5573..5620
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5645..5664
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5852..6646)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5963..5982)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6573..6592
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6745..6764)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6843..7034
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6991..7011
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(7094..7954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7717..7736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7955..8059)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8086..8541
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8173..8192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8383..8404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtggctccct
      841 gcagccggac gcgggcaacg cgagctggaa cgggaccgag gcgccggggg gcggcgcccg
      901 ggccacccct tactccctgc aggtgacgct gacgctggtg tgcctggccg gcctgctcat
      961 gctgctcacc gtgttcggca acgtgctcgt catcatcgcc gtgttcacga gccgcgcgct
     1021 caaggcgccc caaaacctct tcctggtgtc tctggcctcg gccgacatcc tggtggccac
     1081 gctcgtcatc cctttctcgc tggccaacga ggtcatgggc tactggtact tcggcaaggc
     1141 ttggtgcgag atctacctgg cgctcgacgt gctcttctgc acgtcgtcca tcgtgcacct
     1201 gtgcgccatc agcctggacc gctactggtc catcacacag gccaaagagt acaacctgaa
     1261 gcgcacgccg cgccgcatca aggccatcat catcaccgtg tgggtcatct cggccgtcat
     1321 ctccttcccg ccgctcatct ccatcgagaa gaagggcggc ggcggcggcc cgcagccggc
     1381 cgagccgcgc tgcgagatca acgaccagaa gtggtacgtc atctcgtcgt gcatcggctc
     1441 cttcttcgct ccctgcctca tcatgatcct ggtctacgtg cgcatctacc agatcgccaa
     1501 gcgtcgcacc cgcgtgccac ccagccgccg gggtccggac gccgtcgccg cgccgccggg
     1561 gggcaccgag cgcaggccca acggtctggg ccccgagcgc agcgcgggcc cggggggcgc
     1621 agaggccgaa ccgctgccca cccagctcaa cggcgcccct ggcgagcccg cgccggccgg
     1681 gccgcgcgac accgacgcgc tggacctgga ggagggagga aacgtctata tcaaggccga
     1741 caagcagaag aacggcatca aggcgaactt ccacatccgc cacaacatcg aggacggcgg
     1801 cgtgcagctc gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1861 gcccgacaac cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg
     1921 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1981 gctgtacaag ggcggtaccg gagggagcat ggtgagaaag ggcgaggagc tgttcaccgg
     2041 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2101 cggcgagggt gagggcgatg ccaccgaggg caagctgacc ctgaagttca tctgcaccac
     2161 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     2221 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     2281 aggctacatc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2341 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     2401 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacaccggag cagcagcacg
     2461 ctggcgcggg cggcagaacc gcgagaagcg cttcttcttc gtgctggccg tggtcatcgg
     2521 agtgttcgtg gtgtgctggt tccccttctt cttcacctac acgctcacgg ccgtcgggtg
     2581 ctccgtgcca cgcacgctct tcaaattctt cttctggttc ggctactgca acagctcgtt
     2641 gaacccggtc atctacacca tcttcaacca cgatttccgc cgcgccttca agaagatcct
     2701 ctgtcggggg gacaggaagc ggatcgtgtt gtaagctagc gcccctctcc ctcccccccc
     2761 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     2821 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     2881 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     2941 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     3001 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca acgccacgtg
     3061 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     3121 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     3181 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt
     3241 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     3301 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagga taacatggcc
     3361 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     3421 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     3481 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     3541 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     3601 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     3661 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     3721 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     3781 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     3841 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     3901 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     3961 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     4021 gacgagctgt acaaggatgg taaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt
     4081 atgtaagtcg acgaacaaaa actcatctca gaagaggatc tgaatgctgt gggccaggac
     4141 acgcaggagg tcatcgtggt gccacactcc ttgcccttta aggtggtggt gatctcagcc
     4201 atcctggccc tggtggtgct caccatcatc tcccttatca tcctcatcat gctttggcag
     4261 aagaagccac gttaggcggc cgctcgagat cagcctcgac tgtgccttct agttgccagc
     4321 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4381 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4441 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4501 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagtggc ggtaatacgg
     4561 ttatccacag aatcggggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4981 agacgcgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5401 cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc
     5461 gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt
     5521 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac
     5581 gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
     5641 ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc
     5701 atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc
     5761 aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct
     5821 tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     5881 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     5941 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     6001 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     6061 tattcggcaa gcaggcatcg ccataggtca cgacgagatc ctcgccgtcg ggcatgctcg
     6121 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     6181 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     6241 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     6301 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     6361 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     6421 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg
     6481 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     6541 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     6601 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     6661 atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     6721 ttctggaata gctcagaggc cgggcctcgg cctctgcata aataaaaaaa attagtcagc
     6781 catggggcgg agaatgggcg gaactgggcg gagttagggg cggaatgggc ggagttaggg
     6841 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6901 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     6961 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
     7021 tctttccgcc tcaggactct tcctttttca ataaatcaat ctaaagtata tatgagtaaa
     7081 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7141 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7201 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7261 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7321 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7381 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7441 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7501 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7561 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7621 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7681 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7741 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7801 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7861 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7921 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     8101 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8161 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8221 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8281 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8341 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8401 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8461 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     8521 aaatattaac gcttacaatt tac
//