| 质粒名称: | pPSU2 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 89566 |
| 存储实验室: | Song Tan |
|
载体骨架基本信息 |
|
| 载体名称: | pUC9 |
| 空载体大小: | 2635 bp |
| 完整质粒大小: | 7750 bp |
| 载体类型: | Bacterial Expression |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | 750 bp EcoRV fragment/lambda DNA position 650-1399 |
| 物种来源: | phage lambda |
| 插入大小: | 750 bp |
| GenBank编号： | NC_001416.1 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | HindIII(未破坏) |
| 3’克隆位点: | EcoRI(未破坏) |
| 5’测序引物 | N/A |
| 插入基因名称2: | 3000 bp EcoRV fragment、lambda DNA position 25345-26210 |
| 物种来源: | phage lambda |
| 插入大小: | 3000dp |
| GenBank编号： | NC_001416.1 |
| 插入基因名称3: | 4000 bp EcoRV fragment/lambda DNA position 22948-24306 |
| 物种来源: |
phage lambda
Insert Size (bp)4000
|
| 插入大小: | NC_001416.1 |
| 插入基因名称4: | 50 bp PstI fragment/lambda DNA position 26172-26210 |
| 物种来源: | phage lambda |
| 插入大小: | 50bp |
| GenBank编号： | NC_001416.1 |
| 插入基因名称5: | 100 bp PstI fragment/lambda DNA position 2554-2656 |
| 物种来源: | phage lambda |
| 插入大小: | 100cp |
| GenBank编号： | NC_001416.1 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NgoMIV (not destroyed) |
| 3’克隆位点: | None (not destroyed) |
| 5’测序引物 | N/A |
| 3’测序引物 | N/A |
| 插入基因名称6: | 200 bp PstI fragment/lambda DNA 37262-37455 |
| 物种来源: | phage lambda |
| 插入大小: | 200bp |
| GenBank编号： | NC_001416.1 |
| 插入基因名称7: | 300 bp PstI fragment/lambda DNA postion 3856-4149 |
| 物种来源: | phage lambda |
| 插入大小: | 300bp |
| GenBank编号： | NC_001416.1 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | None (not destroyed) |
| 3’克隆位点: | BsrGi (not destroyed) |
| 5’测序引物 | N/A |
| 3’测序引物 | N/A |
| 插入基因名称8: | 400 bp PstI fragment/lambda DNA position 19833-20232 |
| 物种来源: | phage lambda |
| 插入大小: | 400bp |
| GenBank编号： | NC_001416.1 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BglII (not destroyed) |
| 3’克隆位点: | None (not destroyed) |
| 5’测序引物 | N/A |
| 3’测序引物 | N/A |
| 插入基因名称9: | 500 bp PstI fragment/lambda DNA position 11835-12334 |
| 物种来源: | phage lambda |
| 插入大小: | 500bp |
| GenBank编号： | NC_001416.1 |
| 5’测序引物 | N/A |
| 3’测序引物 | N/A |
| 插入基因名称10: | 600 bp PstI fragment/lambda DNA position 2858-3447 |
| 物种来源: | phage lambda |
| 插入大小: | 600bp |
| GenBank编号： | NC_001416.1 |
| 5’克隆位点: | None (not destroyed) |
| 3’克隆位点: | BsrGi (not destroyed) |
| 5’测序引物 | N/A |
| 3’测序引物 | N/A |
| 插入基因名称11: | 1500 bp Psti fragment/lambda DNA position 25340-26170 and 743-1399 |
| 物种来源: | phage lambda |
| 插入大小: | 1500bp |
| GenBank编号： | NC_001416.1 |
| 插入基因名称12: | 4100 bp PstI fragment/lambda DNA position 22948-24306 |
| 物种来源: | phage lambda |
| 插入大小: | 4100bp |
| GenBank编号： | NC_001416.1 |
|
文献引用方法 |
|
| 材料和方法部分: | pPSU2 was a gift from Song Tan (Addgene plasmid # 89566 ; http://n2t.net/addgene:89566 ; RRID:Addgene_89566) |
| 参考文献部分: | The pPSU Plasmids for Generating DNA Molecular Weight Markers. Henrici RC, Pecen TJ, Johnston JL, Tan S. Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1. 10.1038/s41598-017-02693-1 [pii] Pu |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pPSU2 7750 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION High copy number plasmid 2 to prepare Penn State DNA molecular
weight ladders.
ACCESSION .
VERSION .
KEYWORDS pPSU2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7750)
AUTHORS Henrici RC, Pecen TJ, Johnston JL, Tan S
TITLE The pPSU Plasmids for Generating DNA Molecular Weight Markers.
JOURNAL Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.
PUBMED 28550309
REFERENCE 2 (bases 1 to 7750)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7750
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(35..52)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(206..794)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(286..305)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(965..1825)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1588..1607
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1826..1930)
/gene="bla"
/label=AmpR promoter
primer_bind 1998..2016
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2054..2076)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2176..2195
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2389..2411
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2403..2420
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2404..2420
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7554..7570)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7554..7570)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7567..7589)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7578..7594
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7602..7632)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7647..7668
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
1921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
1981 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
2041 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
2101 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
2161 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
2221 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
2281 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
2341 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
2401 gttgtaaaac gacggccagt gaattctcat agatggtcgg tgggaggtgg tacaaattct
2461 ctcatgcaaa aaatatgtaa aatcggtagc aactggaaat cattcaacac ccgcactatc
2521 ggaagttcac cagccagcca cagcacgttc ctgcatacga cgtgtctgcg gctctaccat
2581 atctcctatg agcaacgtgt tagcagagcc aagccacaac tctaatttta atacataatg
2641 aatgataata ataatattaa aaatttcctg tgtaactaat ttactatatg gtttctgata
2701 agaatcattg caaagatcaa acaacttgta ttacattgac agttaagcag ttaattttat
2761 cacctctaaa atatatcagc atctagcatg caacctatca aaatggagag ttttatgact
2821 aaaaaaccat gggaaagaag acttaaagat ttatcgcact tgctcaaatg ctgcattgat
2881 acatattttg accctgaatt atttcgcttg aatttgaatc aattcctcca aaccgcaaga
2941 acagtaacat ttattattca aaaaaacaaa aaccagatta taggatatga catttggtat
3001 aacaataatg ttattgaaaa atggaaaaat gatccattaa tggcttgggc taaaaattct
3061 cgcaatacga tagaaaaaca aggcgattta gaaatgtata gcgaggcaaa ggctactctt
3121 atttcatctt acattgaaga aaatgacatt gagtttatta caaatgaaag tatgttaaac
3181 attggtataa aaaagttagt cagacttgca caaaagaaat taccttcata tttaactgaa
3241 tcatctatta ttaaatcaga aagacgatgg gtcgctaata cgctaaaaga ttacgaatta
3301 ttacatgcct tagctataat ctatggcaga atgtataact gctgtaactc tcttggcata
3361 caaataaaca atccaatggg tgacgatgtg atttcgccaa catcattcga ctctttattt
3421 gatgaagcca ggagaataac ttatttaaaa ttaaaagatt actccataag caaattgtca
3481 tttagcatga tacaatatga caataaaata attcctgaag atattaaaga gcgtctaaaa
3541 ctggtagata agcctaaaaa tatcacttcg acagaagagt tagttgacta tacagccaag
3601 cttgcagaaa cgactttttt aaaggacggt tatcacattc aaacattaat tttttatgat
3661 aaacaattcc atccaattga tttaatcaat acaacatttg aagatcaagc agataaatat
3721 attttttggc gttatgcagc tgacagagcc aaaataacaa atgcctatgg cttcatttgg
3781 atatctgtac actgcagatg tcccagcgcc gcaccgctgt cattaatctg cacaccggca
3841 cggcagttcc ggctgtcgcc ggtattgttc gggttgctga tgcgcttcgg gctgaccatc
3901 cggaactgtg tccggaaaag ccgcgacgaa ctggtatccc aggtggcctg aacgaacagt
3961 tcaccgttaa aggcgtgcat ggccacacct tcccgaatca tcatggtaaa cgtgcgtttt
4021 cgctcaacgt caatgcagca gcagtcatcc tcggcaaact ctttccatgc cgcttcaacc
4081 tcgcgggaaa aggcacgggc ttcttcctcc ccgatgccca gatagcgcca gcttgggcga
4141 tgactgagcc ggaaaaaaga cccgacgata tgatcctgat gcagctggat ggcgttggcg
4201 gcatagccgt tattgcgtac cagatcgtct gcgcgggcat tgccacgggt aaagttgggc
4261 aacagggctg catccacact ttcactcggt gggttccacg accgcaactg ccctccaaat
4321 ccgctgccac cgccgtgata accggcatat tcgcgcagcg atgtcatgcc gtccggcccc
4381 agaagggatc cctgcagttc aggcgctgtt ccgtggcttc ccgttctgcc tgccggtcag
4441 tcagcccccg gcttttcgca tcaatggcgg cccgttttgc ccgttgctgc tgtgcgaatt
4501 tatccgcctg ctgcgccagc gcgttcaggc gctcctgata cgtaaccttg tcgccaagtg
4561 cagccagctg gcgtttgtac tccagcgtct catctttatg cgccagcagg gatttctcct
4621 gtgcagacag ctggcgacgt tgcgccgcct cctccagtac cgcgaactga ctctccgcct
4681 tccacaaatc ccggcgctgc tggctgattt tctcatttgc tccgacatgc ttctccagcg
4741 tccggagttc tgcctgaagc gtcagcaggg cagcatgagc actgtcttcc tgacgatcgc
4801 ccgcagacac cttcacgctg gactgtttcg gctttttcag cgtcgcttca taatcctttt
4861 tcgccgccgc catcagcgtg ttgtaatccg cctgcagttt ttgaggactc tgcggctgcg
4921 gcactttttt ccgcttcagt ggcctttgct gatgccgctt ctgcgccgga ggacgcttcc
4981 tgagctgacg atgcagcctg tccggcggac gtgctggcgg cgcgtgctga gtcagttgca
5041 tcagtcacaa gggccgcgac ctgagcagct gatgcactgg catcgccggc tgatttcttc
5101 gcgtctgccg tactctgtgc caccacggac gcgttacgcg ccacctcttc caccatcagt
5161 tcaagacgac gcagcacctc cggccgggca tcatcctccg tcatggcaca gagaaaatca
5221 ttcagcgtcc ccggttgtga atcttcatac acggtgatgg tcccggcgtg cgatggtgga
5281 aaaccgtcaa cctgcagaga tctggcttcc tgaaaactga agcgcgcttt tgaaggtaac
5341 gtcaccacgc ggcgaacgat ggcctcttcc agccagcaca gaaacatctg gctcgcctga
5401 cgggatgcga cgaattttcg ccgccccata aagtacgccc acgactcgtt cgcactggcc
5461 cgtgccgtgg agtagctcat ctgggcgtaa ttccgggaaa gctgctcata cgagacaccc
5521 agcccggcag cgatataccg cagcagtgac tgctcaaaca cggagtagcc gttatccgta
5581 tcctgagccg tctgcagtga cggaatgtta attctcgttg accctgagca ggctgttgag
5641 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
5701 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
5761 aatgagagtt gttccgttgt ggggaaagtt actgcaggca agttcttcct gtcgcgtcat
5821 tcatcctctc cggataaggc acgggcgtaa tctgccagtg ttttcttgtt ggttgctgca
5881 ccatcctctt cctgcaggcc ggctaaagaa caacctgacc cagcaaaagg tacacaatac
5941 tctgcagttg gactcaagaa tgctgccagc aatagtttaa aatcactagg cgatctccgc
6001 ttagaattca tttcagcatt tattggttgt atgagagtag atagaaaaag acaactctgg
6061 cttgaagcta tcaaaaaact aagtagtgat gaaaactttt caaatatgga actcatcagc
6121 ctcatttcta aatatgaaga gttaagacgt aatgaaccac agattcaagt ggacgatgat
6181 aaattcacta aattgtttta tgacaatatc cagaaatatc tgcttcgaat gagctctgga
6241 catgcaattg ttttatttac tatcacaaga ttagtagatg tcgttggcga aaagtcatta
6301 gttttattcg atgaaccaga ggttcatctg catccacctt tgctctctgc ttttttacga
6361 acattaagcg acttactcga tgcacgcaat ggtgtagcaa taattgcaac tcattcccca
6421 gtagtactgc aagaggttcc aaaatcctgc atgtggaaag tcctacggtc aagagaagca
6481 ataaatatta tccgtccgga tattgagaca ttcggtgaga acttaggtgt tttaactcgt
6541 gaggtgtttt tacttgaagt gacaaattct ggataccacc acttattatc gcagtccgtt
6601 gattcagagc tttcttatga aaccattcta aaaaattata atggtcagat aggattagaa
6661 ggtcgaaccg ttttaaaagc gatgataatg aacagagatg aaggtaaagt acaatgaaaa
6721 aactacctct tccagcgaga acttatagcg aaatgcttaa taaatgctcg gaaagatccg
6781 atatcgcttc actggctgca cgctcaatct gacaggtgcc tctcactttt ggcgtggagc
6841 cacggatgga ctttggccag accgagcctt caatacgctt gtcacccagg aacgtcggag
6901 agccttcctg ttcaatatca tcatcaaaag cagcaagttc atcataaccc gccacatcca
6961 ccgacttttc acggtagttt tttgccgctt taccgcccag gcaccagaag ccacgcccat
7021 tagtgaaacg cttcatggtg agcgtgttat cccggtgctt tttgccatac cacggggcca
7081 gcgccagcag cgacggaata tcacgaatag tcggctcaac gtgggttttc ataaagttct
7141 cggcatcacc atccgttggc aaccagataa gggtgttgcg ctgcttatgc tctataaagt
7201 aggcataaac acccagcagc attttggaat aaccgacacg ggcagacttc accacattca
7261 cctcacggat gtagtcgctg cccatcgcat tcatgatggc ccgctgaaag ggcagtgttt
7321 cccagcgccc ttcctggtat gcggattctt tcgggagata gtaattagca tccgcccatt
7381 caacggcggt ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc
7441 gctgcaggtt aacctgactg ttcgatatat tcactcagca accccggtat cagttcatcc
7501 agcgcggctg ctttgttcat ggctttgatg atatcaagct tggcgtaatc atggtcatag
7561 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
7621 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
7681 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
7741 cgcgcgggga
//
