pDR195

价格:1500元

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载体基本信息

出品公司: Addgene
载体名称: pDR195
质粒类型: 酵母菌表达载体
高拷贝/低拷贝: 高拷贝
启动子: PMA1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6318bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: URA3
备注: --
产品目录号: 36028
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pDR195载体图谱



pDR195多克隆位点

pDR195载体特征

载体简介

载体序列

LOCUS       pDR195	6318 bp 	DNA	SYN
DEFINITION  pDR195
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="pDR195"
                     /mol_type="other DNA"
     terminator      39..350
                     /label="yADH2_terminator"
     misc_feature    complement(43..66)
                     /label="pJG4_5_rev_primer"
     terminator      164..355
                     /label="yADH1_terminator"
     misc_feature    complement(219..243)
                     /label="pHybLex_rev_primer"
     misc_feature    360..515
                     /label="lacZ_a"
     promoter        complement(363..379)
                     /label="M13_forward20_primer"
     misc_feature    complement(372..394)
                     /label="M13_pUC_fwd_primer"
     rep_origin      complement(815..1979)
                     /label="2micron2_origin"
     CDS             complement(2272..3075)
                     /label="ORF frame 1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     gene            complement(2275..3075)
                     /label="URA3"
                     /gene="URA3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             2564..3040
                     /label="ORF frame 2"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        3437..3465
                     /label="AmpR_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     gene            3507..4367
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             3507..4367
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4522..5141
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5450..5479
                     /label="lac_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5493..5515
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5514..5532
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cccagcctcg agcggccgcg cggatccagc tttggacttc ttcgccagag gtttggtcaa
   61 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa
  121 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt
  181 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
  241 ctcttaggtt ttaaaacgar aattcttatt cttgagtaac tctttcctgt aggtcaggtt
  301 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccaat
  361 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
  421 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
  481 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
  541 cttacgcatc tgtgcggtat ttcacaccgc ataatcggat cgtacttgtt acccatcatt
  601 gaattttgaa catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt
  661 ataataatat atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga
  721 aactattgca tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg
  781 tttccatctt gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt
  841 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
  901 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
  961 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
 1021 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
 1081 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
 1141 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
 1201 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
 1261 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
 1321 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
 1381 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
 1441 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
 1501 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
 1561 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
 1621 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
 1681 ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt
 1741 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagcta
 1801 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
 1861 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
 1921 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta
 1981 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc
 2041 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct
 2101 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat
 2161 cgatccgatg ataagctgtc aaacatgaga attgggtaat aactgatata attaaattga
 2221 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
 2281 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
 2341 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
 2401 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
 2461 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
 2521 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
 2581 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
 2641 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
 2701 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
 2761 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
 2821 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
 2881 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
 2941 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
 3001 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
 3061 tgtagctttc gacatgattt atcttcgttt cctgcatgtt tttgttctgt gcagttgggt
 3121 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
 3181 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
 3241 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaagctaatt
 3301 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
 3361 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
 3421 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
 3481 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
 3541 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
 3601 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
 3661 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
 3721 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
 3781 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
 3841 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
 3901 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
 3961 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
 4021 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
 4081 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
 4141 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
 4201 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
 4261 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
 4321 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
 4381 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
 4441 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
 4501 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
 4561 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
 4621 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
 4681 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
 4741 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
 4801 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
 4861 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
 4921 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
 4981 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
 5041 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
 5101 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
 5161 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
 5221 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
 5281 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
 5341 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
 5401 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
 5461 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
 5521 gctatgacca tgattacgcc aagcttcctg aaacggagaa acataaacag gcattgctgg
 5581 gatcacccat acatcactct gttttgcctg accttttccg gtaatttgaa aacaaacccg
 5641 gtctcgaagc ggagatccgg cgataattac cgcagaaata aacccataca cgagacgtag
 5701 aaccagccgc acatggccgg agaaactcct gcgagaattt cgtaaactcg cgcgcattgc
 5761 atctgtattt cctaatgcgg cacttccagg cctcgatcga gaccgtttat ccattgcttt
 5821 tttgttgtct ttttccctcg ttcacagaaa gtctgaagaa gctatagtag aactatgagc
 5881 tttttttgtt tctgttttcc tttttttttt ttttacctct gtggaaattg ttactctcac
 5941 actctttagt tcgtttgttt gttttgttta ttccaattat gaccggtgac gaaacgtggt
 6001 cgatggtggg taccgcttat gctcccctcc attagtttcg attatataaa aaggccaaat
 6061 attgtattat tttcaaatgt cctatcatta tcgtctaaca tctaatttct cttaaatttt
 6121 ttctctttct ttcctataac accaatagtg aaaatctttt tttcttctat atctacaaaa
 6181 actttttttt tctatcaacc tcgttgataa attttttctt taacaatcgt taataattaa
 6241 ttaattggaa aataaccatt ttttctctct tttatacaca cattcaaaag aaagaaaaaa
 6301 aatatacccc agcctcga
//

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