| 质粒名称: | pSi |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 67884 |
| 存储实验室: | Indu Maiti |
|
载体骨架基本信息 |
|
| 载体名称: | N/A |
| 载体出品公司: | Stratagene |
| 完整质粒大小: | 6445bp |
| 载体类型: | 植物表达 |
| 筛选标记: | Kanamycin |
| 载体抗性: | Ampicillin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3') |
| 3’测序引物 | NOSterm-R (5'-attgccaaatgtttgaacga-3') |
|
文献引用方法 |
|
| 材料和方法部分: | pSi was a gift from Indu Maiti (Addgene plasmid # 67884 ; http://n2t.net/addgene:67884 ; RRID:Addgene_67884) |
| 参考文献部分: | pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. Sahoo DK, Dey N, Maiti IB. PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014. PONE-D-14-03371 [pii] PubMed 24897541 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSi 6445 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Derivative of pSiM24 plant expression vector without the M24
promoter.
ACCESSION .
VERSION .
KEYWORDS pSi
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6445)
AUTHORS Sahoo DK, Dey N, Maiti IB
TITLE pSiM24 is a novel versatile gene expression vector for transient
assays as well as stable expression of foreign genes in plants.
JOURNAL PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
eCollection 2014.
PUBMED 24897541
REFERENCE 2 (bases 1 to 6445)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6445
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 67..91
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 283..924
/label=E9 terminator
/note="terminator and polyadenylation signal from the pea
rbcS-E9 gene"
promoter complement(2004..2183)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature 2418..2442
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(2557..3145)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2637..2656)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3316..4176)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3939..3958
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4177..4281)
/gene="bla"
/label=AmpR promoter
rep_origin 4409..4940
/label=oriV
/note="incP origin of replication"
ORIGIN
1 ggcgcgcggt acccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
61 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
121 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccactaga ccagtacctt
181 ggaggcctgt gcgcatccca gggtgggtcc cgtatacccc ggggaattca agcttggatc
241 cctcgaggtt aacacgcgtg tcgacgagct cctgcagtct agagctttcg ttcgtatcat
301 cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca caccagaatc
361 ctactgagtt cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc
421 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
481 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
541 ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt
601 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
661 cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt cagacctaga
721 aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca tttatgaact
781 ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt ataattatag
841 ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt tatgacttgc
901 caattgattg acaacatgca tcaatcgata ctagtctagt aacatagatg acaccgcgag
961 cgataattta tcctagtttg cgagctatat tttgttttct atcgcgtatt aaatgtataa
1021 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
1081 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
1141 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgattcagaa gaactcatcg
1201 aggagcctgt agaaagcaat cctttgggaa tctggagcag caattccgta aagcacaagg
1261 aatctatcag cccactctcc accaagttct tcagcaatat ccctagtagc gagagcaata
1321 tcctgatacc tatcagcaac tccaagcctt ccgcaatcaa tgaatccaga aaaccttccg
1381 ttctccacca taatgtttgg aaggcaagca tcaccatgag taacaacaag atcctcacca
1441 tctggcattc tagccttgag cctagcaaaa agttcagctg gagccaatcc ttgatgttcc
1501 tcatcaagat catcctgatc cacaagtcca gcttccatcc tagttctagc cctttcaatt
1561 ctgtgcttag cttggtgatc aaatgggcaa gtagcaggat caagagtgtg aagccttctc
1621 atagcatcag ccataatgga aaccttctca gctggagcaa ggtgtgaaga aagcaaatct
1681 tgtcctggaa cctctcccaa aagaagccaa tctcttccag cctcagtaac aacatcaaga
1741 acagcagcgc atggaacacc agtagtagca agccaagaaa gtctagcagc ctcatcttga
1801 agctcattaa gagcaccaga aagatcagtc ttaacgaaaa gaactggcct tccctgagca
1861 gaaagcctaa aaacagcagc atcagagcat ccaatagtct gttgagccca atcgtatcca
1921 aaaagcctct caacccaagc agcaggactt ccagcatgaa gtccatcttg ctcaattccc
1981 atttgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
2041 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
2101 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctcag
2161 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
2221 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcagat ctggtgaccc
2281 ctatgtaagg ggccggccat ttaaattcgc gattaattaa cctgtggttg gcatgcacat
2341 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
2401 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
2461 tgaaggcggg aaacgacaat ctgatatcgc gcgcggtacc tgagcaaaag gccagcaaaa
2521 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
2581 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
2641 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
2701 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
2761 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
2821 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
2881 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
2941 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
3001 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
3061 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
3121 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
3181 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
3241 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
3301 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
3361 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
3421 cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga
3481 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
3541 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
3601 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
3661 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
3721 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
3781 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
3841 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
3901 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
3961 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
4021 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
4081 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
4141 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
4201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
4261 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacgggccc cgctcaccgg
4321 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
4381 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa
4441 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg
4501 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc
4561 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag
4621 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg
4681 ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac
4741 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt
4801 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt
4861 taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc
4921 tcgaaccctc ccggcccgct gtcgactacc tagatgtggc gcaacgatgc cggcgacaag
4981 caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc
5041 aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac
5101 gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg
5161 gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc
5221 ggggcaatcc cgcaaggagg gtgaatgaat cgcaccttcg atcgcaaggc gtatcgccag
5281 gaactgatcg acgccggctt ctcggccgaa gatgccgaaa cgatcgcctc gcgcaccgtc
5341 atgcgtgccc cgcgcgaaac cttccagtcg gtcggctcga tggtccagca ggccaccgcc
5401 aagatcgaac gcgattcggt ccagctggcc ccgccggccc ttccggcccc gtcggccgcc
5461 gtcgaacgct cgcgccggct ggaacaggaa gccgccggcc tggccaagtc gatgaccatc
5521 gacacccgcg gcaccatgac caccaagaag cgcaagaccg ccggcgagga tctggccaag
5581 caggtcagcg aagccaagca ggccgccctg ctgaagcata ccaagcagca gatcaaggaa
5641 atgcagctgt cgctgttcga catcgccccg tggccggata ccatgcgggc catgccgaat
5701 gataccgccc gctcggccct gttcaccacc cgcaataaga agatcccgcg cgaagccctc
5761 cagaataagg tcatcttcca cgtcaacaag gatgtcaaga tcacctatac cggcgtcgaa
5821 ctgcgggccg atgatgatga actggtctgg cagcaggtcc tggaatatgc caagcgcacc
5881 ccgatcggcg aaccgatcac cttcaccttc tatgaactgt gccaggatct gggctggtcg
5941 atcaatggcc gctattatac caaggccgaa gaatgcctgt cgcgcctcca ggcgaccgcg
6001 atgggcttca cctcggatcg cgtcggccat ctggaatcgg tcagcctgct gcaccgcttc
6061 cgcgtcctgg atcgcggcaa gaagacctcg cgctgccagg tcctgatcga cgaagaaatc
6121 gtcgtcctgt tcgccggcga tcattatacc aagttcatct gggagaagta tcgcaagctg
6181 tcgccgaccg cccgccgcat gttcgattat ttctcgtcgc accgcgaacc gtatccgctg
6241 aagctggaaa ccttccgcct gatgtgcggc tcggattcga cccgcgtcaa gaagtggcgc
6301 gaacaggtcg gcgaagcctg cgaagaactg cgcggctcgg gcctggtcga acatgcctgg
6361 gtcaatgatg atctggtcca ttgcaagcgc tagggccttg tggggtcagt tccggctggg
6421 ggttcagcca gcgctttacg atatc
//
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