| 质粒名称: | pRN227 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 127222 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pTRANS_201 |
| 载体出品公司: | Cambia |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9, WUS2, AtSTM, sgRNA |
| 物种来源: | S. Pyogenes, Maize, Arabidopsis, synthetic |
| 插入大小: | -- |
| 基因突变: | codon optimized CDSs |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pRN227 was a gift from Daniel Voytas (Addgene plasmid # 127222 ; http://n2t.net/addgene:127222 ; RRID:Addgene_127222) |
| 参考文献部分: | Plant gene editing through de novo induction of meristems. Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF. Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 10.1038/s41587-019-0337-2. 10.1038/s41587-019-0337-2 PubMed 31844292 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRN227 20260 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS.
ACCESSION .
VERSION .
KEYWORDS pRN227
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 20260)
AUTHORS Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
TITLE Plant gene editing through de novo induction of meristems.
JOURNAL Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi:
10.1038/s41587-019-0337-2.
PUBMED 31844292
REFERENCE 2 (bases 1 to 20260)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..20260
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..25
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 103..277
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(334..1131)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(445..464)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 1055..1074
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(1194..1870)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 2061..2082
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2097..2127
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2135..2151
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2140..2162
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2159..2175
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2159..2175
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 3080..3425
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 3459..7562
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7575..7595
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_RNA 8349..8424
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 8527..8706
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 8559..8742
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
polyA_signal 12125..12299
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 13981..14005
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 15306..15935
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 16364..17437
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 17503..17697
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(17933..17955)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 18041..18181
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 18096..18115
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(18196..18213)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(18367..18955)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(18447..18466)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(19042..19836)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta
301 gctagaggat cgatccgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
361 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
421 ccattcgccg ccaagttctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
481 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
541 gatattcggc aagcaggcat cgccatgtgt cacgacgaga tcctcgccgt cgggcatgcg
601 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgttctt cgtccagatc
661 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
721 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
781 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
841 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
901 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga gttcattcag
961 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccgaaa
1021 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
1081 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcccca tggtcgatcg
1141 acagatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
1201 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
1261 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
1321 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
1381 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
1441 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
1501 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
1561 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
1621 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
1681 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
1741 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
1801 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
1861 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
1921 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc
1981 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
2041 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
2101 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
2161 ggaaacagct atgacatgat tacgaattcg agctcaaagt ttaacgcgtt agcagaaggc
2221 atgttgttgt gactccgagg ggttgcctca aactctatct tataaccggc gtggaggcat
2281 ggaggcaggg gtattttggt cattttaata gatagtggaa aatgacgtgg aatttactta
2341 aagacgaagt ctttgcgaca agggggggcc cacgccgaat ttaatattac cggcgtggcc
2401 cccccttatc gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aatttcccgc
2461 ccactagggt taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg
2521 agcgtatatt gtatcaggta tttccgttgg atacgaatta ttcgtacgac cctcggtacc
2581 gatcggcgcg ccagatttgc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
2641 gagaggctta cgcagcaggt atcatcaaga cgatctaccc gagcaataat ctccaggaaa
2701 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
2761 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
2821 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
2881 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
2941 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
3001 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
3061 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
3121 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
3181 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
3241 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
3301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
3361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
3421 gaacacgggg gactcctgca ggtagatcgc tcgtcgacat ggataagaag tactctatcg
3481 gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag tacaaggtgc
3541 catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag aaaaacctta
3601 tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc aagagaaccg
3661 ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag atcttctcta
3721 acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca ttcctcgtgg
3781 aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat gaggtggcat
3841 accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat tctactgata
3901 aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc agaggacact
3961 tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg ttcatccagc
4021 tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca ggtgtggatg
4081 ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac ctcattgctc
4141 agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc tctctcggac
4201 tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc cagctctcaa
4261 aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat cagtacgctg
4321 atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat atcctcagag
4381 tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga tacgatgagc
4441 accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca gagaagtaca
4501 aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat ggtggtgcat
4561 ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat ggaaccgagg
4621 aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc ttcgataacg
4681 gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga aggcaagagg
4741 atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc accttcagaa
4801 tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg atgaccagaa
4861 agtctgagga aaccatcacc ccttggaact tcgaagaggt ggtggataag ggtgctagtg
4921 ctcagtcttt catcgagagg atgaccaact tcgataagaa ccttccaaac gagaaggtgc
4981 tccctaagca ctctttgctc tacgagtact tcaccgtgta caacgagttg accaaggtta
5041 agtacgtgac cgagggaatg aggaagcctg cttttttgtc aggtgagcaa aagaaggcta
5101 tcgttgatct cttgttcaag accaacagaa aggtgaccgt gaagcagctc aaagaggatt
5161 acttcaagaa aatcgagtgc ttcgattcag ttgagatttc tggtgttgag gataggttca
5221 acgcatctct cggaacctac cacgatctcc tcaagatcat taaggataag gatttcttgg
5281 ataacgagga aaacgaggat atcttggagg atatcgttct taccctcacc ctctttgaag
5341 atagagagat gattgaagaa aggctcaaga cctacgctca tctcttcgat gataaggtga
5401 tgaagcagtt gaagagaaga agatacactg gttggggaag gctctcaaga aagctcatta
5461 acggaatcag ggataagcag tctggaaaga caatccttga tttcctcaag tctgatggat
5521 tcgctaacag aaacttcatg cagctcatcc acgatgattc tctcaccttt aaagaggata
5581 tccagaaggc tcaggtttca ggacagggtg atagtctcca tgagcatatc gctaacctcg
5641 ctggatctcc tgcaatcaag aagggaatcc tccagactgt gaaggttgtg gatgagttgg
5701 tgaaggtgat gggaaggcat aagcctgaga acatcgtgat cgaaatggct agagagaacc
5761 agaccactca gaagggacag aagaactcta gggaaaggat gaagaggatc gaggaaggta
5821 tcaaagagct tggatctcag atcctcaaag agcaccctgt tgagaacact cagctccaga
5881 atgagaagct ctacctctac tacctccaga acggaaggga tatgtatgtg gatcaagagt
5941 tggatatcaa caggctctct gattacgatg ttgatcatat cgtgccacag tcattcttga
6001 aggatgattc tatcgataac aaggtgctca ccaggtctga taagaacagg ggtaagagtg
6061 ataacgtgcc aagtgaagag gttgtgaaga aaatgaagaa ctattggagg cagctcctca
6121 acgctaagct catcactcag agaaagttcg ataacttgac taaggctgag aggggaggac
6181 tctctgaatt ggataaggca ggattcatca agaggcagct tgtggaaacc aggcagatca
6241 ctaagcacgt tgcacagatc ctcgattcta ggatgaacac caagtacgat gagaacgata
6301 agttgatcag ggaagtgaag gttatcaccc tcaagtcaaa gctcgtgtct gatttcagaa
6361 aggatttcca attctacaag gtgagggaaa tcaacaacta ccaccacgct cacgatgctt
6421 accttaacgc tgttgttgga accgctctca tcaagaagta tcctaagctc gagtcagagt
6481 tcgtgtacgg tgattacaag gtgtacgatg tgaggaagat gatcgctaag tctgagcaag
6541 agatcggaaa ggctaccgct aagtatttct tctactctaa catcatgaat ttcttcaaga
6601 ccgagattac cctcgctaac ggtgagatca gaaagaggcc actcatcgag acaaacggtg
6661 aaacaggtga gatcgtgtgg gataagggaa gggatttcgc taccgttaga aaggtgctct
6721 ctatgccaca ggtgaacatc gttaagaaaa ccgaggtgca gaccggtgga ttctctaaag
6781 agtctatcct ccctaagagg aactctgata agctcattgc taggaagaag gattgggacc
6841 ctaagaaata cggtggtttc gattctccta ccgtggctta ctctgttctc gttgtggcta
6901 aggttgagaa gggaaagagt aagaagctca agtctgttaa ggaacttctc ggaatcacta
6961 tcatggaaag gtcatctttc gagaagaacc caatcgattt cctcgaggct aagggataca
7021 aagaggttaa gaaggatctc atcatcaagc tcccaaagta ctcactcttc gaactcgaga
7081 acggtagaaa gaggatgctc gcttctgctg gtgagcttca aaagggaaac gagcttgctc
7141 tcccatctaa gtacgttaac tttctttacc tcgcttctca ctacgagaag ttgaagggat
7201 ctccagaaga taacgagcag aagcaacttt tcgttgagca gcacaagcac tacttggatg
7261 agatcatcga gcagatctct gagttctcta aaagggtgat cctcgctgat gcaaacctcg
7321 ataaggtgtt gtctgcttac aacaagcaca gagataagcc tatcagggaa caggcagaga
7381 acatcatcca tctcttcacc cttaccaacc tcggtgctcc tgctgctttc aagtacttcg
7441 atacaaccat cgataggaag agatacacct ctaccaaaga agtgctcgat gctaccctca
7501 tccatcagtc tatcactgga ctctacgaga ctaggatcga tctctcacag ctcggtggtg
7561 attcaagggc tgatcctaag aagaagagga aggtttgacg tcgacgatat gaagatgaag
7621 atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg
7681 gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta
7741 taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca
7801 gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa gataagccag
7861 agctctggtg acggacccat ggcttcgttg aacaacggaa actcgacttg ccttccgcac
7921 aatacatcat ttcttcttag ctttttttct tcttcttcgt tcatacagtt tttttttgtt
7981 tatcagctta cattttcttg aaccgtagct ttcgttttct tctttttaac tttccattcg
8041 gagtttttgt atcttgtttc atagtttgtc ccaggattag aatgattagg catcgaacct
8101 tcaagaattt gattgaataa aacatcttca ttcttaagat atgaagataa tcttcaaaag
8161 gcccctggga atctgaaaga agagaagcag gcccatttat atgggaaaga acaatagtat
8221 ttcttatata ggcccattta agttgaaaac aatcttcaaa agtcccacat cgcttagata
8281 agaaaacgaa gctgagttta tatacagcta gagtcgaagt agtgattgtt ggtagtagcg
8341 actccatggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
8401 gaaaaagtgg caccgagtcg gtgctttttt tcccgggcgc gccgatcatg agcggagaat
8461 taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa
8521 ctgacagaac cgcaacgttg aaggagccac tcagccgcgg gtttctggag tttaatgagc
8581 taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag
8641 ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc
8701 caattagagt ctcatattca ctctcaatcc aaataatctg caccgtacct gcagggtccg
8761 agctaggtca cagaagcgct caggaaggcc gctgagatag aggcatggcg gccaatgcgg
8821 gcggcggtgg agcgggagga ggcagcggca gcggcagcgt ggctgcgccg gcggtgtgcc
8881 gccccagcgg ctcgcggtgg acgccgacgc cggagcagat caggatgctg aaggagctgt
8941 actacggctg cggcatccgg tcgcccagct cggagcagat ccagcgcatc accgccatgc
9001 tgcggcagca cggcaagatc gagggcaaga acgtcttcta ctggttccag aaccacaagg
9061 cccgcgagcg ccagaagcgc cgcctcacca gcctcgacgt gaacgtgccc gccgccggcg
9121 cggccgacgc caccaccagc caactcggcg tcctctcgct gtcgtcgccg ccgccttcag
9181 gcgcggcgcc tccctcgccc accctcggct tctacgccgc cggcaatggc ggcggatcgg
9241 ctgtgctgct ggacacgagt tccgactggg gcagcagcgg cgctgcgatg gccaccgaga
9301 catgcttcct ccaggactac atgggcgtga cggacacggg cagctcgtcg cagtggccac
9361 gcttctcgtc gtcggacacg ataatggcgg cggccgcggc gcgggcggcg acgacgcggg
9421 cgcccgagac tctccctctc ttcccgacct gcggcgacga cggcggcagc ggtagcagca
9481 gctacttgcc gttctggggt gccgcgtcca caactgccgg cgccacttct tccgttgcga
9541 tccagcagca acaccagctg caggagcagt acagctttta cagcaacagc aacagcaccc
9601 agctggccgg caccggcaac caagacgtat cggcaacagc agcagcagcc gccgccctgg
9661 agctgagcct cagctcatgg tgctcccctt accctgctgc agggagtatg tgagagcaac
9721 gcgagctgcc actgctcttc acttatgtct ctggaatgga aggaggagga agtgagcata
9781 gcgttggtgc gttgctgtca ttgtcctagg ttagtagcta gtgccagtta ctagtaagca
9841 tcaggcatag gagtatgtag tagaagcatg cacgttgccg gccagccagg ctttagacgg
9901 gaaaagaatt tggtgcagcc ggctgcaaaa caggatgttt acagcccccc cctcgagccc
9961 tagacttgtc catcttctgg attggccaag ttaattaatg tatgaaataa aaggatgcac
10021 acatagtgac atgctaatca ctataatgtg ggcatcaaag ttgtgtgtta tgtgtaatta
10081 ctaattatct gaataagaga aagagatcat ccatatttct tatcctaaat gaatgtcacg
10141 tgtctttata attctttgat gaaccagatg cattttatta accaattcca tatacatata
10201 aatattaatc atatataatt aatatcaatt gggttagcaa aacaaatcta gtctaggtgt
10261 gttttgctaa ttattggggg atagtgcaaa aagaaatcta cgttctcaat aattcagata
10321 gaaaacttaa taaagtgaga taatttacat agattgcttt tatcctttga tatatgtgaa
10381 accatgcatg atataaggaa aatagataga gaaataattt tttacatcgt tgaatatgta
10441 aacaatttaa ttcaagaagc taggaatata aatattgagg agtttatgat tagagctctc
10501 ccgctggcag acatactgtc ccacaaatga agatggaatc tgtaaaagaa aacgcgtgaa
10561 ataatgcgtc tgacaaaggt taggtcggct gcctttaatc aataccaaag tggtccctac
10621 cacgatggaa aaactgtgca gtcggtttgg ctttttctga cgaacaaata agattcgtgg
10681 ccgacaggtg ggggtccacc atgtgaaggc atcttcagac tccaataatg gagcaatgac
10741 gtaagggctt acgaaataag taagggtagt ttgggaaatg tccactcacc cgtcagtcta
10801 taaatactta gcccctccct cattgttaag ggagcaaaat ctcagagaga tagtcctaga
10861 gagagaaaga gagcaagtag cctagaagta gtcaaggcgg cgaagtattc aggcacgtgg
10921 ccaggaagaa gaaaagccaa gacgacgaaa acaggtaaga gctaagctta tggagagtgg
10981 ttccaacagc acttcttgtc caatggcttt tgccggggat aatagtgatg gtccgatgtg
11041 tcctatgatg atgatgatgc cgcccatcat gacatcacat caacatcatg gtcatgatca
11101 tcaacatcaa caacaagaac atgatggtta tgcatatcag tcacaccacc aacaaagtag
11161 ttcccttttt cttcaatcac tagctcctcc ccaaggaact aagaacaaag ttgcttcttc
11221 ttcttctcct tcctcttgtg ctcctgccta ttctctaatg gagatccatc ataacgaaat
11281 cgttgcagga ggaatcaacc cttgctcctc ttcctcttct tcagcctctg tcaaggccaa
11341 gatcatggct catcctcact accaccgcct cttggccgct tatgtcaatt gtcagaaggt
11401 tggagcacca ccggaggttg tggcgaggct agaggaggca tgctcgtctg ccgcagccgc
11461 tgccgcatct atgggaccaa caggatgtct aggtgaagat ccagggcttg atcaattcat
11521 ggaagcttac tgtgaaatgc tcgttaagta tgagcaagag ctctccaaac ctttcaagga
11581 agctatggtc ttccttcaac gtgtcgagtg tcaattcaaa tccctctctc tatcctcacc
11641 ttcctctttc tccggttatg gagagacagc aattgatagg aacaataatg ggtcatccga
11701 ggaagaagtc gatatgaaca atgaatttgt agatccacaa gctgaggata gagagcttaa
11761 aggacagctc ttgcgcaagt acagtggtta cttagggagc ctcaagcaag agttcatgaa
11821 gaagaggaag aaaggaaagc tccctaaaga agctcgtcaa caactgcttg attggtggag
11881 ccgtcactac aaatggcctt acccttcgga gcaacaaaag ctcgcccttg cggaatcaac
11941 ggggctggac cagaaacaga taaacaattg gttcataaac cagaggaaac ggcattggaa
12001 gccgtcggag gacatgcagt ttgtagtaat ggacgcaaca catcctcacc attacttcat
12061 ggataatgtc ttgggcaatc ctttcccaat ggatcacatc tcctccacca tgctttgact
12121 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
12181 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
12241 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc
12301 cccgaattaa gtgtttgatc gccggcggta ccgagtgtac ttcaagtcag tgggaaatca
12361 ataaaatgat tattttatga atatatttca ttgtgcaagt agatagaaat tacatatgtt
12421 acataacaca cgaaataaac aaaaaaagac aatccaaaaa caaacacccc aaaaaaaata
12481 atcactttag ataaactcgt atgaggagag gcacgttcag tgactcgacg attcccgagc
12541 aaaaaaagtc tccccgtcac acatgtagtg ggtgacgcaa ttatctttaa agtaatcctt
12601 ctgttgactt gtcattgata acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg
12661 gatcccacct tttattttct tcttttttcc atatttaggg ttgacagtga aatcagactg
12721 gcaacctatt aattgcttcc acaatgggac gaacttgaag gggatgtcgt cgatgatatt
12781 ataggtggcg tgttcatcgt agttggtgaa atcgatggta ccgttccaat agttgtgtcg
12841 tccgagactt ctagcccagg tggtctttcc ggtacgagtt ggtccgcaga tgtagaggct
12901 ggggtgtcgg attccattcc ttccattgtc cttgttaaat cggccatcca ttcaaggtca
12961 gattgagctt gttggtatga gacaggatgt atgtaagtat aagcgtctat gcttacatgg
13021 tatagatggg tttccctcca ggagtgtaga tcttcgtggc agcgaagatc tgattctgtg
13081 aagggcgaca catacggttc aggttgtgga gggaataatt tgttggctga atattccagc
13141 cattgaagct ttgttgccca ttcatgaggg aattcttcct tgatcatgtc aagatattcc
13201 tccttagacg ttgcagtctg gataatagtt ctccatcgtg cgtcagattt gcgaggagaa
13261 accttatgat ctcggaaatc tcctctggtt ttaatatctc cgtcctttga tatgtaatca
13321 aggacttgtt tagagtttct agctggctgg atattagggt gatttccttc aaaatcgaaa
13381 aaagaaggat ccctaataca aggtttttta tcaagctgga gaagagcatg atagtgggta
13441 gtgccatctt gatgaagctc agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt
13501 ttctcccaga gaaactggaa taaatcatct ctttgagatg agcacttggg ataggtaagg
13561 aaaacatatt tagattggag tctgaagttc ttactagcag aaggcatgtt gttgtgactc
13621 cgaggggttg cctcaaactc tatcttataa ccggcgtgga ggcatggagg caggggtatt
13681 ttggtcattt taatagatag tggaaaatga cgtggaattt acttaaagac gaagtctttg
13741 cgacaagggg gggcccacgc cgaatttaat attaccggcg tggccccccc ttatcgcgag
13801 tgctttagca cgagcggtcc agatttaaag tagaaaattt cccgcccact agggttaaag
13861 gtgttcacac tataaaagca tatacgatgt gatggtattt gatggagcgt atattgtatc
13921 aggtatttcc gttggatacg aattattcgt acgaccctca tagtttaaac tatcagtgtt
13981 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
14041 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
14101 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
14161 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac
14221 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
14281 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
14341 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
14401 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
14461 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
14521 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
14581 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
14641 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
14701 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
14761 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
14821 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
14881 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
14941 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
15001 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
15061 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg
15121 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
15181 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
15241 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
15301 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
15361 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
15421 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
15481 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
15541 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
15601 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
15661 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
15721 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
15781 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
15841 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
15901 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
15961 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
16021 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
16081 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
16141 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
16201 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
16261 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc
16321 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc
16381 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
16441 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
16501 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
16561 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
16621 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
16681 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
16741 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
16801 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
16861 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
16921 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
16981 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
17041 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
17101 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
17161 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
17221 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
17281 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
17341 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
17401 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
17461 aattgcccta gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat
17521 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
17581 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
17641 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
17701 gtctggccag cgcacagccc aagagctgca aaaagcgcct acccttcggt cgctgcgctc
17761 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
17821 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
17881 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
17941 acatgcagct cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
18001 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
18061 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
18121 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
18181 gccctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
18241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
18301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
18361 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
18421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
18481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
18541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
18601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
18661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
18721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
18781 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
18841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
18901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
18961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
19021 tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat attttatttt
19081 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
19141 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc
19201 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc
19261 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc
19321 atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc
19381 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt
19441 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc
19501 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc
19561 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac
19621 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt
19681 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt
19741 atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag
19801 ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta
19861 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
19921 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
19981 gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg
20041 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
20101 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
20161 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
20221 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
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