pMETαA

价格:1500元

联系方式:I47-825O-882O

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pMETalpha A载体基本信息

出品公司: Invitrogen
载体名称: pMETalpha A, pMETα A, pMETalphaA, pMETαA
质粒类型: 毕赤酵母蛋白表达载体(methanoloica酵母)
表达水平: 高拷贝
诱导方法: 甲醇诱导
启动子: AUG1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8043 bp
5' 测序引物及序列: AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´;  alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´
3' 测序引物及序列: AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´
载体标签: C-V5, C-His
载体抗性: 氨苄
筛选标记: ADE2
备注: 利用甲醇诱导蛋白在methanoloica酵母中表达。
产品目录号: K1780-01
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pMETalpha A载体质粒图谱和多克隆位点信息

pMETalpha A载体图谱

pMETalpha A多克隆位点

pMETalpha A载体简介

pMETalpha A载体序列

LOCUS       pMET\alpha\A            8043 bp    DNA     circular      1-AUG-2013
DEFINITION  pMETα A.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTNAME|pMET alpha A|
COMMENT     VNTOAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    1106..1130
                     /vntifkey="21"
                     /label=AUG1_fwd_primer
     misc_feature    complement(1662..1683)
                     /vntifkey="21"
                     /label=AUG1_rev_primer
     CDS             3085..3660
                     /vntifkey="4"
                     /label=ORF\frame\3
     CDS             3666..4799
                     /vntifkey="4"
                     /label=ORF\frame\2
     CDS             4963..5961
                     /vntifkey="4"
                     /label=ORF\frame\3
     gene            complement(6774..7634)
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             complement(6774..7634)
                     /vntifkey="4"
                     /label=ORF\frame\2
     promoter        complement(7676..7704)
                     /vntifkey="30"
                     /label=AmpR_promoter
     sig_peptide     1197..1463
                     /vntifkey="94"
                     /label=Alpha\Factor\Secretion\Signal
BASE COUNT     2360 a      1741 c      1568 g      2374 t 
ORIGIN
        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag 
       61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt 
      121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac 
      181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca 
      241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac 
      301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa 
      361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc 
      421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa 
      481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg 
      541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc 
      601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa 
      661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct 
      721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct 
      781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg 
      841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa 
      901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat 
      961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct 
     1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt 
     1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac 
     1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga 
     1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag 
     1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact 
     1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg 
     1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc 
     1441 tcgagaaaag agaggctgaa gctgaattca cgtggcccag ccggccgtgg atccacgcgt 
     1501 cgtcgacccg cggcggccgc cagcttccta ggggtaagcc tatccctaac cctctcctcg 
     1561 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgaactagt atacaattct 
     1621 agggctgcct gtttggatat ttttataatt tttgagagtt tgccaactaa tgtttttctc 
     1681 ttctatgata tttatcatgt agttggatat ctttttttct ttgaaaagtt tttatttagt 
     1741 tctttctctc ttttttataa tgttttcagt ttctttcctt tatattgaaa atacgatgtt 
     1801 ctttgtcttt cttttttaaa tcctcaatta taatgtgtac cgtttgatca acttgtaaaa 
     1861 ttgtttgtag tcctgtaatc ttaattgacg attggttgat agtttacccc agtatattgt 
     1921 gcggctgaga tattacccga ttcggaagat tgattttttc aacagacata aacccatagg 
     1981 agatttagta cagcgacata tgctaattgc ctgataatgc tcactaacat tttctccttt 
     2041 taatctggta tatgcataca ggtatgttga tatggccttg ttgatcaaag ctacgaaagt 
     2101 gtagactatt tcccaaacag cccccaacca ccacctcaca cgcgcacact gcgaagtgaa 
     2161 agcgctccct tattttcttc tccgctggtt caaaacaaaa aagaatctaa tctgtagaca 
     2221 cacgccccgt aaaacccaat cttcatcatc gtaattttca gtgacggacg atcgatcaac 
     2281 catatagttt gttagtgact gagcttcagt agttgacctc ttgaagaaat aacgtcttga 
     2341 acaacatact ttatacctac caacattcgc catcaacaac agtgtgacac tgacataact 
     2401 ttacatccat tcatcgacaa aaaaatgtct ctagttcgga ttcatgctta tgggttagct 
     2461 atttcgccca atgtgtccat ctgacattac tattttgcat tttaatttaa ttagaacttg 
     2521 actagcgcac taccagcata tcatctcatt tccgtaaata ccaaatgtat tatatattga 
     2581 aagcttttga ccaggttatt ataaaagaaa cttcatgctc gaaaaagatc atttcgaaaa 
     2641 gttgcctagt ttcatgaaat tttaaagcag tttatataaa ttttaccttt tgatgcggaa 
     2701 ttgacttttt cttgaataat acataacttt tcttaaaaga atcaaagaca gataaaattt 
     2761 aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat aacactgaca 
     2821 tctttaacaa cttttaatta tgatacattt cttacgtcat gattgattat tacagctatg 
     2881 ctgacaaatg actcttgttg catggctacg aaccgggtaa tactaagtga ttgactcttg 
     2941 ctgacctttt attaagaact aaatggacaa tattatggag catttcatgt ataaattggt 
     3001 gcgtaaaatc gttggatctc tcttctaagt acatcctact ataacaatca agaaaaacaa 
     3061 gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt aggaggggga 
     3121 caattgggac gtatgattgt tgaggcagca aacaggctca acattaagac ggtaatacta 
     3181 gatgctgaaa attctcctgc caaacaaata agcaactcca atgaccacgt taatggctcc 
     3241 ttttccaatc ctcttgatat cgaaaaacta gctgaaaaat gtgatgtgct aacgattgag 
     3301 attgagcatg ttgatgttcc tacactaaag aatcttcaag taaaacatcc caaattaaaa 
     3361 atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca aaaagagcat 
     3421 ttaatcaaaa atggtatagc agttacccaa agtgttcctg tggaacaagc cagtgagacg 
     3481 tccctattga atgttggaag agatttgggt tttccattcg tcttgaagtc gaggactttg 
     3541 gcatacgatg gaagaggtaa cttcgttgta aagaataagg aaatgattcc ggaagctttg 
     3601 gaagtactga aggatcgtcc tttgtcgccg aaaaatgggc accatttact aaagaattag 
     3661 cagtcatgat tgtgagatct gttaacggtt tagtgttttc ttacccaatt gtagagacta 
     3721 tccacaagga caatatttgt gacttatgtt atgcgcctgc tagagttccg gactccgttc 
     3781 aacttaaggc gaagttgttg gcagaaaatg caatcaaatc ttttcccggt tgtggtatat 
     3841 ttggtgtgga aatgttctat ttagaaacag gggaattgct tattaacgaa attgccccaa 
     3901 ggcctcacaa ctctggacat tataccattg atgcttgcgt cacttctcaa tttgaagctc 
     3961 atttgagatc aatattggat ttgccaatgc caaagaattt cacatctttc tccaccatta 
     4021 caacgaacgc cattatgcta aatgttcttg gagacaaaca tacaaaagat aaagagctag 
     4081 aaacttgcga aagagcattg gcgactccag gttcctcagt gtacttatat ggaaaagagt 
     4141 ctagacctaa cagaaaagta ggtcacataa atattattgc ctccagtatg gcggaatgtg 
     4201 aacaaaggct gaactacatt acaggtagaa ctgatattcc aatcaaaatc tctgtcgctc 
     4261 aaaagttgga cttggaagca atggtcaaac cattggttgg aatcatcatg ggatcagact 
     4321 ctgacttgcc ggtaatgtct gccgcatgtg cggttttaaa agattttggc gttccatttg 
     4381 aagtgacaat agtctctgct catagaactc cacataggat gtcagcatat gctatttccg 
     4441 caagcaagcg tggaattaaa acaattatcg ctggagctgg tggggctgct cacttgccag 
     4501 gtatggtggc tgcaatgaca ccacttcctg tcatcggtgt gcccgtaaaa ggttcttgtc 
     4561 tagatggagt agattcttta cattcaattg tgcaaatgcc tagaggtgtt ccagtagcta 
     4621 ccgtcgctat taataatagt acgaacgctg cgctgttggc tgtcagactg cttggcgctt 
     4681 atgattcaag ttatacaacg aaaatggaac agtttttatt aaagcaagaa gaagaagttc 
     4741 ttgtcaaagc acaaaagtta gaaactgtcg gttacgaagc ttatctagaa aacaagtaat 
     4801 atataagttt attgatatac ttgtacagca aataattata aaatgatata cctatttttt 
     4861 aggctttgtt atgattacat caaatgtgga cttcatacat agaaatcaac gcttacaggt 
     4921 gtcctttttt aagaatttca tacataagat ctactagaat caatgttaaa cctgttatca 
     4981 ttagaacagc catctccaga aggttctatc caagtgaaaa ctttggtctt caagccaaag 
     5041 actgctaaaa cacaaacccc agtcccaatt attgttgttg ctgaaacttc tacttctact 
     5101 ccgggtggtg ttatagccaa agcttcgggc actaaagagc caagattagc aaacgatgaa 
     5161 ttgaccaacc aattctttgg ttctcctgct aaagagtttt ctattgccag cttatcaaac 
     5221 gaacacaaag acggtaaagt caagatcatt attgatgctg ggatcaaaga tgatgactcg 
     5281 actaattacg ttatttccaa cactgttgct aagttagttt taaccggtaa gcaattatta 
     5341 ggtttcttat ccaactacga accaaccatc gttgatttta ctgctgaagc tgcaaccgct 
     5401 gccccaactc cagttgttaa gaaagaacct gctgcatctg gtaagcttga tgatgccaaa 
     5461 ttaattggtg ttactgctga caaggaaaaa gatttctcca actggtactc ccaaattcta 
     5521 atgaagggtg aaatgttaga ctactacgat gtctctggtt gttacattat gagaccagct 
     5581 tcttacacaa tttgggaaaa gattcaatct tggttcgatt cgaagattaa agaagatggt 
     5641 gttactaatg cttatttccc aatgtttgtc tcatccaagg ttttagaaaa ggaaaaggac 
     5701 cacatcgatg gtttcgctcc tgaagttgct tgggttacca gagctggtaa atctgaatta 
     5761 gaagaaccaa ttgctattag accaacttct gaaactgtta tgggtaccct gcaggggccg 
     5821 gccttaatta aggcgcgccc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 
     6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
     7741 acatttcccc gaaaagtgcc acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga 
     7801 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 
     7861 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 
     7921 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 
     7981 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcaagggc gatggcccac 
     8041 tac 
//

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