| 质粒名称: | pCK53 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000586 |
| 产品用途: | Mammalian cAMP-responsive SEAP expression vector (PCRE-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | pCRE |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pCK53
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCK53载体质粒基本信息
pCK53质粒图谱载体图谱和pCK53载体序列质粒序列多克隆位点信息
pCK53质粒载体简介
pCK53质粒序列载体序列
LOCUS pCK53 4893 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION KF528989
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4893)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4893
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 207..255
/note="synthetic polyadenylation signal"
misc_feature 269..360
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
misc_feature 394..633
/label=CRE promoter
CDS 637..2222
/codon_start=1
/label=SEAP CDS
/translation="SFESRIRPPCCCCCCCWA*GYSSPWASSQLRRRTRTSGTARQPRP
WVPPRSCSLHRQPPRTSSSSWAMGWGCLR*QLPGS*KGRRRTNWGLRYPWPWTASHMWL
CPRHTM*TNMCQTVEPQPRPTCAGSRATSRPLA*VQPPALTSATRHAATRSSP**IGPR
KQGSQWEW*PPHECSTPRQPAPTPTR*TATGTRTPTCLPRPARRGARTSLRSSSPTWTL
T*S*VEAESTCFAWEPQTLSTQMTTAKVGPGWTGRIWCRNGWRSARVPGMCGTALSSCR
LPWTRL*PISWVSLSLET*NTRSTETPHWTPP*WR*QRLPCAC*AGTPAASSSSWRVVA
STMVIMKAGLTGH*LRRSCSTTPLRGRASSPARRTR*ASSLPTTPTSSPSEATPCEGAP
SSGWPLARPGTGRPTRSSYTETVQAMCSRTAPGRMLPRARAGAPSIGSSQQCPWTKRPT
QARTWRCSRAARRRTWFTACRSRPS*RTSWPSPPAWSPTPPATWRPPPAPPTPRTRVTL
ESGRPAASSRH"
CDS 637..2165
/codon_start=1
/product="secreted alkaline phosphatase from human
placenta"
/label=SEAP
/note="The natural enzyme has a C-terminal transmembrane
sequence that is replaced by a GPI anchor."
/translation="SFESRIRPPCCCCCCCWA*GYSSPWASSQLRRRTRTSGTARQPRP
WVPPRSCSLHRQPPRTSSSSWAMGWGCLR*QLPGS*KGRRRTNWGLRYPWPWTASHMWL
CPRHTM*TNMCQTVEPQPRPTCAGSRATSRPLA*VQPPALTSATRHAATRSSP**IGPR
KQGSQWEW*PPHECSTPRQPAPTPTR*TATGTRTPTCLPRPARRGARTSLRSSSPTWTL
T*S*VEAESTCFAWEPQTLSTQMTTAKVGPGWTGRIWCRNGWRSARVPGMCGTALSSCR
LPWTRL*PISWVSLSLET*NTRSTETPHWTPP*WR*QRLPCAC*AGTPAASSSSWRVVA
STMVIMKAGLTGH*LRRSCSTTPLRGRASSPARRTR*ASSLPTTPTSSPSEATPCEGAP
SSGWPLARPGTGRPTRSSYTETVQAMCSRTAPGRMLPRARAGAPSIGSSQQCPWTKRPT
QARTWRCSRAARRRTWFTACRSRPS*RTSWPSPPAWSPTPPATWRPPPAPP"
CDS 638..663
/codon_start=1
/label=SEAP CDS
/translation="FESRIRPP"
CDS 638..663
/codon_start=1
/product="secreted alkaline phosphatase from human
placenta"
/label=SEAP
/note="The natural enzyme has a C-terminal transmembrane
sequence that is replaced by a GPI anchor."
/translation="FESRIRPP"
misc_feature 727..732
/label=SDS
misc_feature 1252..1257
/label=SDS
misc_feature 1753..1758
/label=SDS
misc_feature 1883..1888
/label=SDS
polyA_signal 2223..2344
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 2581..3521
/label=ColE1
/note="ColE1"
rep_origin complement(2763..3351)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3522..4382)
/label=ampR CDS
promoter complement(4383..4487)
/gene="bla"
/label=AmpR promoter
rep_origin join(4514..4893,1..76)
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
61 ttaacgttta caatttccca ttcgccattc aggctgcgca actgttggga agggcgatcg
121 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag
181 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg
241 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
301 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
361 atcgataggt accgagctct tacgcgtgct agcgcaccag acagtgacgt cagctgccag
421 atcccatggc cgtcatactg tgacgtcttt cagacacccc attgacgtca atgggagaac
481 agatctgccg ccccgactgc atctgcgtgt tcgaattcgc caatgacaag acgctgggcg
541 gggtttgtgt catcatagaa ctaaagacat gcaaatatat ttcttccggg gacaccgcca
601 gcaaacgcga gcaacgggcc acggggatga agcagaagct tcgaatcgcg aattcgccca
661 ccatgctgct gctgctgctg ctgctgggcc tgaggctaca gctctccctg ggcatcatcc
721 cagttgagga ggagaacccg gacttctgga accgcgaggc agccgaggcc ctgggtgccg
781 ccaagaagct gcagcctgca cagacagccg ccaagaacct catcatcttc ctgggcgatg
841 ggatgggggt gtctacggtg acagctgcca ggatcctaaa agggcagaag aaggacaaac
901 tggggcctga gatacccctg gccatggacc gcttcccata tgtggctctg tccaagacat
961 acaatgtaga caaacatgtg ccagacagtg gagccacagc cacggcctac ctgtgcgggg
1021 tcaagggcaa cttccagacc attggcttga gtgcagccgc ccgctttaac cagtgcaaca
1081 cgacacgcgg caacgaggtc atctccgtga tgaatcgggc caagaaagca gggaagtcag
1141 tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc agccggcacc tacgcccaca
1201 cggtgaaccg caactggtac tcggacgccg acgtgcctgc ctcggcccgc caggaggggt
1261 gccaggacat cgctacgcag ctcatctcca acatggacat tgacgtgatc ctaggtggag
1321 gccgaaagta catgtttcgc atgggaaccc cagaccctga gtacccagat gactacagcc
1381 aaggtgggac caggctggac gggaagaatc tggtgcagga atggctggcg aagcgccagg
1441 gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc ttccctggac ccgtctgtga
1501 cccatctcat gggtctcttt gagcctggag acatgaaata cgagatccac cgagactcca
1561 cactggaccc ctccctgatg gagatgacag aggctgccct gcgcctgctg agcaggaacc
1621 cccgcggctt cttcctcttc gtggagggtg gtcgcatcga ccatggtcat catgaaagca
1681 gggcttaccg ggcactgact gagacgatca tgttcgacga cgccattgag agggcgggcc
1741 agctcaccag cgaggaggac acgctgagcc tcgtcactgc cgaccactcc cacgtcttct
1801 ccttcggagg ctaccccctg cgagggagct ccatcttcgg gctggcccct ggcaaggccc
1861 gggacaggaa ggcctacacg gtcctcctat acggaaacgg tccaggctat gtgctcaagg
1921 acggcgcccg gccggatgtt accgagagcg agagcgggag ccccgagtat cggcagcagt
1981 cagcagtgcc cctggacgaa gagacccacg caggcgagga cgtggcggtg ttcgcgcgcg
2041 gcccgcaggc gcacctggtt cacggcgtgc aggagcagac cttcatagcg cacgtcatgg
2101 ccttcgccgc ctgcctggag ccctacaccg cctgcgacct ggcgcccccc gccggcacca
2161 ccgacgccgc gcacccgggt tactctagag tcggggcggc cggccgcttc gagcagacat
2221 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
2281 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
2341 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
2401 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga
2461 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
2521 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
2581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
2641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
2701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
2761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
2821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
2881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
2941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
3001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
3061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
3121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
3181 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
3241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
3301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
3361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
3421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
3481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
3541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
3601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
3661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
3721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
3781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
3841 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
3901 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
3961 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
4021 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
4081 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
4141 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
4201 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
4261 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
4321 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
4381 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
4441 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
4501 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4561 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
4621 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4681 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
4741 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4801 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
4861 tcttttgatt tataagggat tttgccgatt tcg
//
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