| 质粒名称: | pLTR-G |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17532 |
| 存储实验室: | Jakob Reiser |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 (Clontech) |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 3417 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | VSV-G glycoprotein (Indiana strain) |
| 物种来源: | vesicular stomatitis virus |
| 插入大小: | 2415 bp |
| 基因突变: | -- |
| GenBank编号 | J02428 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLTR-G was a gift from Jakob Reiser (Addgene plasmid # 17532 ; http://n2t.net/addgene:17532 ; RRID:Addgene_17532) |
| 参考文献部分: | Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles. Reiser J, Harmison G, Kluepfel-Stahl S, Brady RO, Karlsson S, Schubert M. Proc Natl Acad Sci U S A. 1996 Dec 24. 93(26):15266-71. 10.1073/pnas.93.26.15266 PubMed 8986799 |
买家导航
pLTRG载体基本信息
pLTRG载体图谱质粒图谱和多克隆位点信息
pLTRG载体简介
pLTRG载体序列
LOCUS pLTR-G 5832 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLTR-G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5832)
AUTHORS Reiser J, Harmison G, Kluepfel-Stahl S, Brady RO, Karlsson S,
Schubert M
TITLE Transduction of nondividing cells using pseudotyped defective
high-titer HIV type 1 particles.
JOURNAL Proc Natl Acad Sci U S A. 1996 Dec 24. 93(26):15266-71.
PUBMED 8986799
REFERENCE 2 (bases 1 to 5832)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5832
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 782..2317
/codon_start=1
/product="vesicular stomatitis virus G glycoprotein"
/label=VSV-G
/note="Indiana strain"
/translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
polyA_signal 2620..2741
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2657..2676)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2711..2730
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2748..3203)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2885..2906)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3097..3116
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3230..3334
/gene="bla"
/label=AmpR promoter
promoter 3336..3693
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3359..3379)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3544..3679
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3606..3625
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3728..4522
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3782..3801)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4392..4411
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4710..4729)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4754..4801
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5130..5718
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5619..5638
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattac cggactcaga tctcgagacc tagaaaaaca tggagcaatc acaagtagca
61 atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag gaagaggtgg
121 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
181 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac
241 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
301 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
361 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
421 ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg aggtttgaca
481 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
541 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
601 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcctgt
661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
721 ccactgctta agcctcaata aagcttctag agatccctcg acctcgagga attctgacac
781 tatgaagtgc cttttgtact tagccttttt attcattggg gtgaattgca agttcaccat
841 agtttttcca cacaaccaaa aaggaaactg gaaaaatgtt ccttctaatt accattattg
901 cccgtcaagc tcagatttaa attggcataa tgacttaata ggcacagcct tacaagtcaa
961 aatgcccaag agtcacaagg ctattcaagc agacggttgg atgtgtcatg cttccaaatg
1021 ggtcactact tgtgatttcc gctggtatgg accgaagtat ataacacatt ccatccgatc
1081 cttcactcca tctgtagaac aatgcaagga aagcattgaa caaacgaaac aaggaacttg
1141 gctgaatcca ggcttccctc ctcaaagttg tggatatgca actgtgacgg atgccgaagc
1201 agtgattgtc caggtgactc ctcaccatgt gctggttgat gaatacacag gagaatgggt
1261 tgattcacag ttcatcaacg gaaaatgcag caattacata tgccccactg tccataactc
1321 tacaacctgg cattctgact ataaggtcaa agggctatgt gattctaacc tcatttccat
1381 ggacatcacc ttcttctcag aggacggaga gctatcatcc ctgggaaagg agggcacagg
1441 gttcagaagt aactactttg cttatgaaac tggaggcaag gcctgcaaaa tgcaatactg
1501 caagcattgg ggagtcagac tcccatcagg tgtctggttc gagatggctg ataaggatct
1561 ctttgctgca gccagattcc ctgaatgccc agaagggtca agtatctctg ctccatctca
1621 gacctcagtg gatgtaagtc taattcagga cgttgagagg atcttggatt attccctctg
1681 ccaagaaacc tggagcaaaa tcagagcggg tcttccaatc tctccagtgg atctcagcta
1741 tcttgctcct aaaaacccag gaaccggtcc tgctttcacc ataatcaatg gtaccctaaa
1801 atactttgag accagataca tcagagtcga tattgctgct ccaatcctct caagaatggt
1861 cggaatgatc agtggaacta ccacagaaag ggaactgtgg gatgactggg caccatatga
1921 agacgtggaa attggaccca atggagttct gaggaccagt tcaggatata agtttccttt
1981 atacatgatt ggacatggta tgttggactc cgatcttcat cttagctcaa aggctcaggt
2041 gttcgaacat cctcacattc aagacgctgc ttcgcaactt cctgatgatg agagtttatt
2101 ttttggtgat actgggctat ccaaaaatcc aatcgagctt gtagaaggtt ggttcagtag
2161 ttggaaaagc tctattgcct cttttttctt tatcataggg ttaatcattg gactattctt
2221 ggttctccga gttggtatcc atctttgcat taaattaaag cacaccaaga aaagacagat
2281 ttatacagac atagagatga accgacttgg aaagtaactc aaatcctgca caacagattc
2341 ttcatgtttg gaccaaatca acttgtgata ccatgctcaa agaggcctca attatatttg
2401 agtttttaat ttttatgaaa aaaaaaaaaa aaaacggaat tcctcgaggg atcttccata
2461 cctaccagtt ctgcgcctgc aggtcgcggc cgatccaccg gatctagata actgatcata
2521 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
2581 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
2641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
2701 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
2761 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
2821 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
2881 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
2941 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
3001 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
3061 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
3121 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
3181 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
3241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
3301 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
3361 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3421 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
3481 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
3541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
3601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
3661 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
3721 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
3781 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
3841 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
3901 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
3961 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
4021 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
4081 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
4141 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
4201 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
4261 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
4321 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
4381 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
4441 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
4501 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
4561 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
4621 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
4681 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
4741 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
4801 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
4861 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
4921 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
4981 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
5041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
5101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
5161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
5221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
5281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
5341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
5401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
5461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
5521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
5581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
5641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
5701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
5761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
5821 accgccatgc at
//
