RNAi-Ready pSIREN-RetroQ-DsRed-Express

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: RNAi-Ready pSIREN-RetroQ-DsRed-Express
质粒类型: 哺乳动物细胞自失活基因沉默逆病毒载体,shRNA
高拷贝/低拷贝: 低拷贝
启动子: Human U6
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6543 bp
5' 测序引物及序列: U6-F: ATGGACTATCATATGCTTACCGTA (Clontech )
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
备注: 载体表达DsRed红色荧光蛋白作为基因沉默标志。
产品目录号: 632487
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

RNAi-Ready pSIREN-RetroQ-DsRed-Express载体图谱



RNAi-Ready pSIREN-RetroQ-DsRed-Express多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

载体简介

载体序列

LOCUS       RNAi-Ready_pSIREN-RetroQ-DsRed-Express   6543 bp    DNA                       12-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 632487
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    4710..5436
                     /label=5'-LTR\(CMV/MSV)
     misc_feature    5466..6275
                     /label=Extended\packaging\signal
     misc_feature    1415..1840
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(3487..4347)
                     /label=Amp(R)
     misc_feature    2120..2406
                     /label=SV40\origin/promoter
     promoter        6291..6543
                     /label=Human\U6\promoter
     rep_origin      complement(2726..3325)
                     /label=pUC\ori
     rep_origin      2341..2406
                     /label=SV40\origiin
     promoter        6..594
                     /label=CMV\IE\Promoter
     CDS             618..1295
                     /label=DsRed-Express
                     /note="CDS dsRedExpress"
     misc_feature    6543..1
                     /label=shRNA\Cloning\Site
BASE COUNT     1567 a      1744 c      1630 g      1602 t 
ORIGIN
        1 aattctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 
      541 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag 
      601 cgctaccggt cgccaccatg gcctcctccg aggacgtcat caaggagttc atgcgcttca 
      661 aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 
      721 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc 
      781 ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc 
      841 accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc 
      901 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg 
      961 acggctcctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg 
     1021 taatgcagaa gaagactatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg 
     1081 gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa ggacggcggc cactacctgg 
     1141 tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg 
     1201 tggactccaa gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg 
     1261 agcgcgccga gggccgccac cacctgttcc tgtagctcga gatatcagtg gtccaggctc 
     1321 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat 
     1381 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg 
     1441 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag 
     1501 agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag aaccatcaga 
     1561 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc 
     1621 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac 
     1681 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc 
     1741 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc 
     1801 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg 
     1861 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc tgcctcgcgc 
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 
     2041 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag atccggctgt 
     2101 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     2161 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 
     2221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 
     2281 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     2341 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     2401 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta actatgcggc 
     2461 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 
     2521 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     2581 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     2641 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     2701 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     2761 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     2821 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     2881 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     2941 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     3001 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     3061 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     3121 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     3181 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     3241 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     3301 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     3361 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     3421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     3481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     3541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     3601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     3661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     3721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     3781 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     3841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     3901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     3961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     4021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     4081 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 
     4141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     4201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     4261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     4321 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     4381 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     4441 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 
     4501 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattagc 
     4561 ttggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc 
     4621 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 
     4681 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
     4741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
     4801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
     4861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 
     4921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 
     4981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat 
     5041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
     5101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 
     5161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
     5221 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cttccgatag actgcgtcgc 
     5281 ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg tggtctcgct 
     5341 gttccttggg agggtctcct ctgagtgatt gactacccac gacgggggtc tttcatttgg 
     5401 gggctcgtcc gggatttgga gacccctgcc cagggaccac cgacccacca ccgggaggta 
     5461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgtt tgatgttatg 
     5521 cgcctgcgtc tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact 
     5581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt 
     5641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg 
     5701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg 
     5761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt 
     5821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc 
     5881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta 
     5941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc 
     6001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc 
     6061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc 
     6121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct 
     6181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc 
     6241 tccctttatc cagccctcac tccttctcta ggcgccggaa ttgaagatct gggcaggaag 
     6301 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga 
     6361 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa 
     6421 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata 
     6481 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac 
     6541 gag 
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng