pEBVHisC

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pEBVHisC
质粒类型: 哺乳动物表达载体;附加体载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: RSV
载体大小: 10029 bp
5' 测序引物及序列: pREP Forward 5’ GCTCGATACAATAAACGCC 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: His tag (N-term)
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α
宿主细胞(系): 人类细胞;灵长类细胞;犬类细胞
备注: pEBVHisC载体是附加体型载体,可以在基因组外自主复制,不需要整合和转座到基因组上,无需包装成病毒,即可实现稳定表达。其它附加体载体有:  
   pREP4
   PCEP4
   pREP7
   pREP10
   pEBNA-DEST
   pEBVHisA
   pEBVHisB
   pEBVHisC
   pREP4/CAT
   pEBVHis-lacZ
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEBVHisC载体图谱



pEBVHisC多克隆位点

pEBVHis载体特征

载体简介

载体序列

LOCUS       pEBVHisC	10315 bp 	DNA	SYN
DEFINITION  pEBVHisC
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="pEBVHisC"
                     /mol_type="other DNA"
     misc_feature    112..134
                     /label="pGEX_3_primer"
     terminator      279..410
                     /label="SV40_PA_terminator"
     misc_feature    367..386
                     /label="EBV_rev_primer"
     misc_feature    complement(506..529)
                     /label="Xpress_EK"
     misc_feature    complement(533..565)
                     /label="T7_leader"
     misc_feature    complement(548..566)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(632..650)
                     /label="pREP_fwd_primer"
     CDS             1211..1948
                     /label="ORF frame 2"
                     /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
                     NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
                     HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
                     RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
                     VPAPDRTIASHRPCAQAASSKLPSTKL*"
     terminator      complement(1469..1739)
                     /label="TK_PA_terminator"
                     /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
                     NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
                     HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
                     RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
                     VPAPDRTIASHRPCAQAASSKLPSTKL*"
     CDS             complement(1747..2772)
                     /label="ORF frame 2"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            complement(1750..2757)
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             2014..3384
                     /label="ORF frame 1"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     promoter        2891..3039
                     /label="TATA_promoter"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     gene            3046..3384
                     /label="tet (852 - 1190)"
                     /gene="tet (852 - 1190)"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     CDS             3288..3899
                     /label="ORF frame 3"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     gene            complement(4333..5193)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     CDS             complement(4333..5193)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5235..5263)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             complement(5819..7033)
                     /label="ORF frame 1"
                     /translation="MAFYLEGACAVETRMMMTDYWDSWASFLHVHDLSPWLFHDFPPW
                     LFHVLYPGGLHYLLDPGLHYLLDPGLHCLLDPGLHLLLLPLLLLPLLLLLPLLPLLLL
                     PLLPLLLLPLLPLLLLPLLPLLLLLPLLPLLLLLPLLPLLLLPLLPLLLLPLLPLLLL
                     PLLLLPLLLLPLLLLPLLPLLPLLLLLPLLLLPLLPLLPLLLLPLLLLLPLLPLLPLL
                     LLLPLLPLLLLLPLLLLLPLLPLLPLLLLLPLLPLLLLLPLLLLLPLLPLLPLLLLLP
                     LLLLLPLLPLLPLLPLLLLLPLLLLLPLLLLPLPLLLLLLFHRGSLCSQCNLDVFGVS
                     GHHLYVLALILSRPGLLVFRLLVLVLFPVLVHGYHPLFFEVHCRRSLLVQMCLPSLLG
                     HFQVLYLAPRQT*"
     CDS             5821..7746
                     /label="ORF frame 1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG
                     RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG
                     AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG
                     GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA
                     GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG
                     GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS
                     GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG
                     SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP
                     RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF
                     VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL
                     QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG
                     DDGDEGGDGDEGEEGQE*"
     CDS             5936..7048
                     /label="ORF frame 2"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
                     GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
                     EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
                     GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
                     EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
                     VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
     rep_origin      8050..10220
                     /label="EBV_origin"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
                     GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
                     EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
                     GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
                     EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
                     VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
ORIGIN
    1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
   61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
  121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
  181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
  241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
  301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
  361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
  421 tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
  481 gatctcgagg tcggatccat cgatccttat cgtcatcgtc gtacagatcc cgacccattt
  541 gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa ccccccatgg
  601 aggtgcacac caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta
  661 aatacaatta tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc
  721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
  781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
  841 aagtgtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
  901 tgaggggact cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt
  961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
 1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
 1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
 1141 cggcagcaga gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa
 1201 gctgtcaaac atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg
 1261 ggggtcgggg gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg
 1321 gtccatgccc aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag
 1381 accccacgca acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga
 1441 ccccgtccct aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct
 1501 gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg
 1561 gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
 1621 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
 1681 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
 1741 tctcccctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
 1801 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
 1861 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
 1921 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
 1981 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
 2041 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
 2101 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
 2161 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
 2221 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
 2281 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
 2341 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
 2401 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
 2461 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
 2521 gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
 2581 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
 2641 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
 2701 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
 2761 aggctttttc atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg
 2821 tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac
 2881 ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg
 2941 ggcggggttt gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc
 3001 gccagcaaac gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg
 3061 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
 3121 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
 3181 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
 3241 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
 3301 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
 3361 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
 3421 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
 3481 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc
 3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
 3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
 3661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
 3721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
 3781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
 3841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
 3901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
 3961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
 4021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
 4081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
 4141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
 4201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
 4261 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
 4321 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
 4381 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
 4441 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
 4501 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
 4561 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
 4621 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
 4681 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
 4741 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
 4801 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
 4861 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
 4921 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
 4981 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
 5041 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
 5101 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
 5161 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
 5221 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
 5281 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
 5341 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
 5401 attctcatgt ttgacagctt atcatcgcga taagctgatc ctcacaggcc gcacccagct
 5461 tttcttccgt tgccccagta gcatctctgt ctggtgacct tgaagaggaa gaggaggggt
 5521 cccgagaatc cccatcccta ccgtccagca aaaaggggga cgaggaattt gaggcctggc
 5581 ttgaggctca ggacgcaaat cttgaggatg ttcagcggga gttttccggg ctgcgagtaa
 5641 ttggtgatga ggacgaggat ggttcggagg atggggaatt ttcagacctg gatctgtctg
 5701 acagcgacca tgaaggggat gagggtgggg gggctgttgg agggggcagg agtctgcact
 5761 ccctgtattc actgagcgtc gtctaataaa gatgtctatt gatctctttt agtgtgaatc
 5821 atgtctgacg aggggccagg tacaggacct ggaaatggcc taggagagaa gggagacaca
 5881 tctggaccag aaggctccgg cggcagtgga cctcaaagaa gagggggtga taaccatgga
 5941 cgaggacggg gaagaggacg aggacgagga ggcggaagac caggagcccc gggcggctca
 6001 ggatcagggc caagacatag agatggtgtc cggagacccc aaaaacgtcc aagttgcatt
 6061 ggctgcaaag ggacccacgg tggaacagga gcaggagcag gagcgggagg ggcaggagca
 6121 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg
 6181 gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga
 6241 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
 6301 ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg
 6361 gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
 6421 ggggcaggag caggaggggc aggaggggca ggaggggcag gagcaggagg ggcaggagca
 6481 ggaggagggg caggaggggc aggaggggca ggagcaggag gggcaggagc aggaggggca
 6541 ggagcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg ggcaggaggg
 6601 gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
 6661 ggaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
 6721 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
 6781 ggggcaggag caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga
 6841 ggtagtggag gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga
 6901 gccagggggg gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg
 6961 cccaggagtc ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca
 7021 ggtagaaggc catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa
 7081 ggtggcccag atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat
 7141 gacccaggag aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa
 7201 aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac
 7261 attgcagaag gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa
 7321 ggaacttggg tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta
 7381 aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc
 7441 tttggaatgg cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt
 7501 tatttcatgg tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag
 7561 gaccttgtta tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt
 7621 gacgatggag tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag
 7681 ggtgatgacg gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag
 7741 gagtgatgta acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc
 7801 actaaagaat aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg
 7861 tcttgtcacc attttcgtcc tcccaacatg gggcaattgg gcatacccat gttgtcacgt
 7921 cactcagctc cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt
 7981 gagcaatcag acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag
 8041 caaccagcat gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg
 8101 catatgcaaa ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc
 8161 atgaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag
 8221 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa
 8281 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
 8341 attaggatag cctatgctac ccagatatag attaggatag catatgctac ccagatatag
 8401 attaggatag catatgctat ccagatattt gggtagtata tgctacccag atataaatta
 8461 ggatagcata tactacccta atctctatta ggatagcata tgctacccgg atacagatta
 8521 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
 8581 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
 8641 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
 8701 ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc
 8761 accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg
 8821 cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc
 8881 cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg
 8941 accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca
 9001 aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa
 9061 gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg
 9121 gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt
 9181 tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta
 9241 ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg
 9301 agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata
 9361 ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat
 9421 tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc
 9481 acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta
 9541 ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc
 9601 ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa
 9661 tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct
 9721 taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct
 9781 attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt
 9841 ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
 9901 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
 9961 gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca
10021 taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac
10081 tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc
10141 tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc
10201 aaccaactga agggattaca tgcactgccc cgaatacaaa acaaaagcgc tcctcgtacc
10261 agcgaagaag gggcagagat gccgtagtca ggtttagttc gtccggcggc ggggc

//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng