pREP7

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pREP7
质粒类型: 哺乳动物表达载体;附加体载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: RSV
载体大小: 9494 bp
5' 测序引物及序列: pREP Forward 5’ GCTCGATACAATAAACGCC 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α
宿主细胞(系): 人类细胞;灵长类细胞;犬类细胞
备注:
pREP7载体是附加体型载体,可以在基因组外自主复制,类似的载体有:
   pREP4
   PCEP4
   pREP7
   pREP10
   pEBNA-DEST
   pEBVHisA
   pEBVHisB
   pEBVHisC
   pREP4/CAT
   pEBVHis-lacZ
 
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pREP7载体图谱



pREP7多克隆位点

载体简介

载体序列

LOCUS       pREP7	9494 bp 	DNA	SYN
DEFINITION  pREP7
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="pREP7"
                     /mol_type="other DNA"
     misc_feature    583..601
                     /label="pREP_fwd_primer"
     misc_feature    complement(717..736)
                     /label="EBV_rev_primer"
     misc_feature    complement(969..991)
                     /label="pGEX_3_primer"
     CDS             1084..1818
                     /label="ORF frame 1"
                     /translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN
                     HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH
                     PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR
                     VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV
                     PAPDRTIASHRPCAQAASSKLPSTKL*"
     terminator      complement(1339..1609)
                     /label="TK_PA_terminator"
                     /translation="MTGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSKN
                     HGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGLH
                     PNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIAR
                     VPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPTV
                     PAPDRTIASHRPCAQAASSKLPSTKL*"
     CDS             complement(1617..2642)
                     /label="ORF frame 1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            complement(1620..2627)
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             1884..3254
                     /label="ORF frame 3"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     promoter        2761..2909
                     /label="TATA_promoter"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     gene            2916..3254
                     /label="tet (852 - 1190)"
                     /gene="tet (852 - 1190)"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     CDS             3158..3769
                     /label="ORF frame 2"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     gene            complement(4203..5063)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     CDS             complement(4203..5063)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5105..5133)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             5804..6925
                     /label="ORF frame 2"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR
                     GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD
                     VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR
                     ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA
                     PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD
                     GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*"
     rep_origin      7229..9399
                     /label="EBV_origin"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEGGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERAR
                     GRGRGRGEKRPRSPSSQSSSSGSPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPD
                     VPPGAIEQGPADDPGEGPSTGPRGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLR
                     ALLARSHVERTTDEGTWVAGVFVYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMA
                     PGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDD
                     GVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGEEGQE*"
ORIGIN
    1 tctagagtcg accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg
   61 gtgcactctc agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc
  121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
  181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
  241 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg
  301 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg
  361 gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt
  421 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
  481 tggtacgatc gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc
  541 actgaattcc gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc
  601 catttgacca ttcaccacat tggtgtgcac ctccaagctg ggtaccagct gctagcaagc
  661 ttgctagcgg ccgctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg
  721 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
  781 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
  841 gcattcattt tatgtttcag gttcaggggg aggtggggag gttttttaaa gcaagtaaaa
  901 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
  961 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
 1021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
 1081 gccatgaccg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg ggggtcgggg
 1141 gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc
 1201 aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag accccacgca
 1261 acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct
 1321 aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct gcgagccctg
 1381 ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc
 1441 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
 1501 tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
 1561 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccctat
 1621 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
 1681 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
 1741 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
 1801 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
 1861 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
 1921 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
 1981 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
 2041 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
 2101 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
 2161 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
 2221 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
 2281 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
 2341 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
 2401 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
 2461 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
 2521 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
 2581 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
 2641 atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg
 2701 gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc
 2761 gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt
 2821 gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac
 2881 gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg ggctacgtct
 2941 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
 3001 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
 3061 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
 3121 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
 3181 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
 3241 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
 3301 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
 3361 atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc caggaaccgt
 3421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
 3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
 3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
 3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
 3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
 3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
 3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
 3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
 3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
 3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
 4021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
 4081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
 4141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
 4201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
 4261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
 4321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
 4381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
 4441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
 4501 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
 4561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
 4621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
 4681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
 4741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
 4801 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
 4861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
 4921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
 4981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
 5041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
 5101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
 5161 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
 5221 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
 5281 ttgacagctt atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg
 5341 ccccagtagc atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc
 5401 catccctacc gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg
 5461 acgcaaatct tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg
 5521 acgaggatgg ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg
 5581 aaggggatga gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac
 5641 tgagcgtcgt ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag
 5701 gggccaggta caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa
 5761 ggctccggcg gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga
 5821 agaggacgag gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca
 5881 agacatagag atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg
 5941 acccacggtg gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga
 6001 gggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg gaggccgccg
 6061 gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg ggagaggtcg
 6121 tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat ccgggtctcc
 6181 accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg aagccgatta
 6241 ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc cgggagcgat
 6301 agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc ggggtcaggg
 6361 tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc aaggaggttc
 6421 caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta ggagtcacgt
 6481 agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg gaggtagtaa
 6541 gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat gtcgtcttac
 6601 accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac ctggcccgct
 6661 aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat ttgctgaggt
 6721 tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct gcaatatcag
 6781 ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc cacctatggt
 6841 ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag gtgatggaga
 6901 tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca atcgtattaa
 6961 aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg cgtgctgttg
 7021 gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg ggcaattggg
 7081 catacccatg ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca
 7141 ttagcgacat ttacctggtg agcaatcaga catgcgacgg ctttagcctg gcctccttaa
 7201 attcacctaa gaatgggagc aaccagcatg caggaaaagg acaagcagcg aaaattcacg
 7261 cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
 7321 atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
 7381 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
 7441 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
 7501 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
 7561 atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
 7621 gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat
 7681 gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat
 7741 gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat
 7801 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
 7861 gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta
 7921 cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac
 7981 cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc
 8041 gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt
 8101 tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac
 8161 acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac
 8221 tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg
 8281 agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt
 8341 ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc
 8401 ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc
 8461 atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg
 8521 atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag
 8581 gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa
 8641 attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact
 8701 gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag
 8761 attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg
 8821 gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt
 8881 gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc
 8941 ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg
 9001 ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg
 9061 ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag
 9121 cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc
 9181 ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct
 9241 gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca
 9301 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
 9361 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gaatacaaaa
 9421 caaaagcgct cctcgtacca gcgaagaagg ggcagagatg ccgtagtcag gtttagttcg
 9481 tccggcggcg gggc
//

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