pEBVHisB

价格:3000元

联系方式:I47-825O-882O

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pEBVHisB载体基本信息

出品公司: Invitrogen
载体名称: pEBVHisB
质粒类型: 哺乳动物表达载体;附加体载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: RSV
载体大小: 10019 bp
5' 测序引物及序列: pREP Forward 5’ GCTCGATACAATAAACGCC 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: His tag (N-term)
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α 等
宿主细胞(系): 人类细胞;灵长类细胞;犬类细胞
备注:
pEBVHis-lacZ载体是附加体型载体,可以在基因组外自主复制,不需要整合和转座到基因组上,无需包装成病毒,即可实现稳定表达。其它附加体载体有:  
   pREP4
   PCEP4
   pREP7
   pREP10
   pEBNA-DEST
   pEBVHisA
   pEBVHisB
   pEBVHisC
   pREP4/CAT
   pEBVHis-lacZ
 
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pEBVHisB载体质粒图谱和多克隆位点信息

pEBVHis B载体图谱



pEBVHis B多克隆位点

pEBVHisB载体简介


索取pEBVHis A,B,C载体说明书及蛋白表达与纯化的操作手册请E.Mail我们,或者在线留言。

pEBVHisB载体序列

LOCUS       pEBVHisB	10307 bp 	DNA	SYN
DEFINITION  pEBVHisB
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="pEBVHisB"
                     /mol_type="other DNA"
     misc_feature    112..134
                     /label="pGEX_3_primer"
     terminator      279..410
                     /label="SV40_PA_terminator"
     misc_feature    367..386
                     /label="EBV_rev_primer"
     misc_feature    complement(498..521)
                     /label="Xpress_EK"
     misc_feature    complement(525..557)
                     /label="T7_leader"
     misc_feature    complement(540..558)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(624..642)
                     /label="pREP_fwd_primer"
     CDS             1203..1940
                     /label="ORF frame 3"
                     /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
                     NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
                     HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
                     RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
                     VPAPDRTIASHRPCAQAASSKLPSTKL*"
     terminator      complement(1461..1731)
                     /label="TK_PA_terminator"
                     /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
                     NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
                     HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
                     RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
                     VPAPDRTIASHRPCAQAASSKLPSTKL*"
     CDS             complement(1739..2764)
                     /label="ORF frame 2"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            complement(1742..2749)
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             2006..3376
                     /label="ORF frame 2"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     promoter        2883..3031
                     /label="TATA_promoter"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     gene            3038..3376
                     /label="tet (852 - 1190)"
                     /gene="tet (852 - 1190)"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     CDS             3280..3891
                     /label="ORF frame 1"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     gene            complement(4325..5185)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     CDS             complement(4325..5185)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5227..5255)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             complement(5811..7025)
                     /label="ORF frame 1"
                     /translation="MAFYLEGACAVETRMMMTDYWDSWASFLHVHDLSPWLFHDFPPW
                     LFHVLYPGGLHYLLDPGLHYLLDPGLHCLLDPGLHLLLLPLLLLPLLLLLPLLPLLLL
                     PLLPLLLLPLLPLLLLPLLPLLLLLPLLPLLLLLPLLPLLLLPLLPLLLLPLLPLLLL
                     PLLLLPLLLLPLLLLPLLPLLPLLLLLPLLLLPLLPLLPLLLLPLLLLLPLLPLLPLL
                     LLLPLLPLLLLLPLLLLLPLLPLLPLLLLLPLLPLLLLLPLLLLLPLLPLLPLLLLLP
                     LLLLLPLLPLLPLLPLLLLLPLLLLLPLLLLPLPLLLLLLFHRGSLCSQCNLDVFGVS
                     GHHLYVLALILSRPGLLVFRLLVLVLFPVLVHGYHPLFFEVHCRRSLLVQMCLPSLLG
                     HFQVLYLAPRQT*"
     CDS             5813..7738
                     /label="ORF frame 2"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG
                     RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG
                     AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG
                     GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA
                     GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG
                     GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS
                     GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG
                     SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP
                     RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF
                     VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL
                     QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG
                     DDGDEGGDGDEGEEGQE*"
     CDS             5928..7040
                     /label="ORF frame 3"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
                     GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
                     EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
                     GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
                     EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
                     VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
     rep_origin      8042..10212
                     /label="EBV_origin"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
                     GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
                     EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
                     GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
                     EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
                     VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
ORIGIN
    1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
   61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
  121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
  181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
  241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
  301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
  361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
  421 tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
  481 gatctcgagc tcggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca
  541 ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccccat ggaggtgcac
  601 accaatgtgg tgaatggtca aatggcgttt attgtatcga gctaggcact taaatacaat
  661 tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg tctgttgcct
  721 tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt
  781 ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagtgtat
  841 tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat cctgagggga
  901 ctcctaaccg cgtacaaccg aagccccgct gggcgcctaa acacacccta gtcccctcag
  961 atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
 1021 ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
 1081 tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcagca
 1141 gagcagattg tactgagagt gcaccataca agctcaggat ctgcgatgat aagctgtcaa
 1201 acatgagaat tggtcgacca ctgggcgcca gaaatccgcg cggtggtttt tgggggtcgg
 1261 gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg ccagtacatg cggtccatgc
 1321 ccaggccatc caaaaaccat gggtctgtct gctcagtcca gtcgtggacc agaccccacg
 1381 caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg gaccccgtcc
 1441 ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
 1501 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
 1561 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
 1621 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
 1681 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccct
 1741 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
 1801 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
 1861 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
 1921 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
 1981 gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
 2041 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
 2101 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
 2161 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
 2221 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
 2281 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
 2341 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
 2401 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
 2461 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
 2521 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
 2581 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
 2641 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
 2701 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
 2761 tcatatctca ttgcccggga tctgcggcac gctgttgacg ctgttaagcg ggtcgctgca
 2821 gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg acctgggacc
 2881 gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc tgggcggggt
 2941 ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca ccgccagcaa
 3001 acgcgagcaa cgggccacgg ggatgaagca gggcatggcg gccgacgcgc tgggctacgt
 3061 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
 3121 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
 3181 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
 3241 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
 3301 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
 3361 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
 3421 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
 3481 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcagcaaaag gccaggaacc
 3541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
 3601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
 3661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
 3721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
 3781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
 3841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
 3901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
 3961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
 4021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
 4081 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
 4141 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
 4201 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
 4261 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
 4321 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
 4381 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
 4441 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
 4501 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
 4561 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
 4621 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
 4681 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
 4741 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
 4801 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
 4861 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
 4921 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
 4981 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
 5041 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
 5101 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
 5161 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
 5221 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
 5281 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
 5341 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat
 5401 gtttgacagc ttatcatcgc gataagctga tcctcacagg ccgcacccag cttttcttcc
 5461 gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa
 5521 tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct
 5581 caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat
 5641 gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac
 5701 catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat
 5761 tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga
 5821 cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc
 5881 agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg
 5941 gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg
 6001 gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa
 6061 agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc
 6121 aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg
 6181 ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg
 6241 agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc
 6301 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
 6361 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg
 6421 agcaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg
 6481 ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg
 6541 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
 6601 aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg
 6661 ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc
 6721 aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg
 6781 agcaggaggt ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg
 6841 aggccggggt cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg
 6901 gggaagtcgt gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag
 6961 tcccagtagt cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag
 7021 gccatttttc caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc
 7081 agatggtgag cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg
 7141 agaaggccca agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg
 7201 gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga
 7261 aggtttaaga gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg
 7321 ggtcgccggt gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg
 7381 aactgccctt gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat
 7441 ggcccctgga cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat
 7501 ggtcttttta caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt
 7561 tatgacaaag cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg
 7621 agtagatttg cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga
 7681 cggagatgac ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg
 7741 taacttgtta ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga
 7801 ataaatcccc agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca
 7861 ccattttcgt cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc
 7921 tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
 7981 agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
 8041 atgcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca
 8101 aaggatagca ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat
 8161 agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
 8221 agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
 8281 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
 8341 agcctatgct acccagatat agattaggat agcatatgct acccagatat agattaggat
 8401 agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat taggatagca
 8461 tatactaccc taatctctat taggatagca tatgctaccc ggatacagat taggatagca
 8521 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
 8581 tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
 8641 tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
 8701 tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct
 8761 ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg
 8821 tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact
 8881 tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt
 8941 ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca
 9001 gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc
 9061 acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta
 9121 gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata
 9181 gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc
 9241 agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac
 9301 atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat
 9361 gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca
 9421 tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa
 9481 ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt tattctatta
 9541 gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct
 9601 tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc
 9661 ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc
 9721 ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt
 9781 agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt
 9841 aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag
 9901 ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg
 9961 gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca cataaaggca
10021 atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc actcagtgtt
10081 ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc cctttgtgtt
10141 tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta ccaaccaact
10201 gaagggatta catgcactgc cccgaataca aaacaaaagc gctcctcgta ccagcgaaga
10261 aggggcagag atgccgtagt caggtttagt tcgtccggcg gcggggc
//

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