pEBVHis-lacZ

价格:3000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pEBVHis-lacZ
质粒类型: 哺乳动物表达载体;附加体载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: RSV
载体大小: 13328 bp
5' 测序引物及序列: pREP Forward 5’ GCTCGATACAATAAACGCC 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: His tag (N-term)
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α
宿主细胞(系): 人类细胞;灵长类细胞;犬类细胞
备注:
pEBVHis-lacZ载体是附加体型载体,可以在基因组外自主复制,不需要整合和转座到基因组上,无需包装成病毒,即可实现稳定表达。其它附加体载体有:  
   pREP4
   PCEP4
   pREP7
   pREP10
   pEBNA-DEST
   pEBVHisA
   pEBVHisB
   pEBVHisC
   pREP4/CAT
   pEBVHis-lacZ
 
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEBVHis-lacZ载体图谱

载体简介

载体序列

LOCUS       Exported               13328 bp ds-DNA     circular SYN 13-AUG-2016
DEFINITION  synthetic circular DNA
KEYWORDS    pEBVHis-lacz
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Biofeng Lab.
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    276..410
                     /note="SV40 poly(A)"
                     /note="SV40 polyadenylation signal"
     CDS             complement(453..3500)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /note="lacZ"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     CDS             complement(3519..3542)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /note="Xpress(TM) tag"
                     /translation="DLYDDDDK"
     CDS             complement(3546..3578)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(3582..3599)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     promoter        complement(3615..3877)
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     polyA_signal    4647..4695
                     /note="HSV TK poly(A)"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4760..5785)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5825..5970)
                     /note="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(6587..7175)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7346..8206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8207..8311)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      11065..12854
                     /note="oriP"
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
       61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
      181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
      241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
      421 tgccggcctc gatcgagcgg ccgctagcaa gcttattttt gacaccagac caactggtaa
      481 tggtagcgac cggcgctcag ctggaattcc gccgatactg acgggctcca ggagtcgtcg
      541 ccaccaatcc ccatatggaa accgtcgata ttcagccatg tgccttcttc cgcgtgcagc
      601 agatggcgat ggctggtttc catcagttgc tgttgactgt agcggctgat gttgaactgg
      661 aagtcgccgc gccactggtg tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg
      721 ttttcgctcg ggaagacgta cggggtatac atgtctgaca atggcagatc ccagcggtca
      781 aaacaggcgg cagtaaggcg gtcgggatag ttttcttgcg gccctaatcc gagccagttt
      841 acccgctctg ctacctgcgc cagctggcag ttcaggccaa tccgcgccgg atgcggtgta
      901 tcgctcgcca cttcaacatc aacggtaatc gccatttgac cactaccatc aatccggtag
      961 gttttccggc tgataaataa ggttttcccc tgatgctgcc acgcgtgagc ggtcgtaatc
     1021 agcaccgcat cagcaagtgt atctgccgtg cactgcaaca acgctgcttc ggcctggtaa
     1081 tggcccgccg ccttccagcg ttcgacccag gcgttagggt caatgcgggt cgcttcactt
     1141 acgccaatgt cgttatccag cggtgcacgg gtgaactgat cgcgcagcgg cgtcagcagt
     1201 tgttttttat cgccaatcca catctgtgaa agaaagcctg actggcggtt aaattgccaa
     1261 cgcttattac ccagctcgat gcaaaaatcc atttcgctgg tggtcagatg cgggatggcg
     1321 tgggacgcgg cggggagcgt cacactgagg ttttccgcca gacgccactg ctgccaggcg
     1381 ctgatgtgcc cggcttctga ccatgcggtc gcgttcggtt gcactacgcg tactgtgagc
     1441 cagagttgcc cggcgctctc cggctgcggt agttcaggca gttcaatcaa ctgtttacct
     1501 tgtggagcga catccagagg cacttcaccg cttgccagcg gcttaccatc cagcgccacc
     1561 atccagtgca ggagctcgtt atcgctatga cggaacaggt attcgctggt cacttcgatg
     1621 gtttgcccgg ataaacggaa ctggaaaaac tgctgctggt gttttgcttc cgtcagcgct
     1681 ggatgcggcg tgcggtcggc aaagaccaga ccgttcatac agaactggcg atcgttcggc
     1741 gtatcgccaa aatcaccgcc gtaagccgac cacgggttgc cgttttcatc atatttaatc
     1801 agcgactgat ccacccagtc ccagacgaag ccgccctgta aacggggata ctgacgaaac
     1861 gcctgccagt atttagcgaa accgccaaga ctgttaccca tcgcgtgggc gtattcgcaa
     1921 aggatcagcg ggcgcgtctc tccaggtagc gaaagccatt ttttgatgga ccatttcggc
     1981 acagccggga agggctggtc ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtg
     2041 gccgtggtgt cggctccgcc gccttcatac tgcaccgggc gggaaggatc gacagatttg
     2101 atccagcgat acagcgcgtc gtgattagcg ccgtggcctg attcattccc cagcgaccag
     2161 atgatcacac tcgggtgatt acgatcgcgc tgcaccattc gcgttacgcg ttcgctcatc
     2221 gccggtagcc agcgcggatc atcggtcaga cgattcattg gcaccatgcc gtgggtttca
     2281 atattggctt catccaccac atacaggccg tagcggtcgc acagcgtgta ccacagcgga
     2341 tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt tctgcttcat cagcaggata
     2401 tcctgcacca tcgtctgctc atccatgacc tgaccatgca gaggatgatg ctcgtgacgg
     2461 ttaacgcctc gaatcagcaa cggcttgccg ttcagcagca gcagaccatt ttcaatccgc
     2521 acctcgcgga aaccgacatc gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc
     2581 agttcaacca ccgcacgata gagattcggg atttcggcgc tccacagttt cgggttttcg
     2641 acgttcagac gtagtgtgac gcgatcggca taaccaccac gctcatcgat aatttcaccg
     2701 ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccataaaga aactgttacc
     2761 cgtaggtagt cacgcaactc gccgcacatc tgaacttcag cctccagtac agcgcggctg
     2821 aaatcatcat taaagcgagt ggcaacatgg aaatcgctga tttgtgtagt cggtttatgc
     2881 agcaacgaga cgtcacggaa aatgccgctc atccgccaca tatcctgatc ttccagataa
     2941 ctgccgtcac tccaacgcag caccatcacc gcgaggcggt tttctccggc gcgtaaaaat
     3001 gcgctcaggt caaattcaga cggcaaacga ctgtcctggc cgtaaccgac ccagcgcccg
     3061 ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc
     3121 tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg attctccgtg
     3181 ggaacaaacg gcggattgac cgtaatggga taggttacgt tggtgtagat gggcgcatcg
     3241 taaccgtgca tctgccagtt tgaggggacg acgacagtat cggcctcagg aagatcgcac
     3301 tccagccagc tttccggcac cgcttctggt accggaaacc aggcaaagcg ccattcgcca
     3361 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     3421 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     3481 tcacgacgtt gtaaaacgac gggatctatc attggatcct tatcgtcatc gtcgtacaga
     3541 tcccgaccca tttgctgtcc accagtcatg ctagccatac catgatgatg atgatgatga
     3601 gaacccccca tggaggtgca caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg
     3661 agctaggcac ttaaatacaa ttatctctgc aatgcggaat tcagtggttc gtccaatcca
     3721 tgtcagacct gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa
     3781 tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat
     3841 gttgcaagac tacaagtgta ttgcataaga ctacatttcc ccctccctat gcaaaagcga
     3901 aactactata tcctgagggg actcctaacc gcgtacaacc gaagccccgc tgggcgccta
     3961 aacacaccct agtcccctca gatacgcgta tatctggccc gtacatcgcg aagcagcgca
     4021 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
     4081 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
     4141 tggcttaact atgcggcagc agagcagatt gtactgagag tgcaccatac aagctcagga
     4201 tctgcgatga taagctgtca aacatgagaa ttggtcgacc actgggcgcc agaaatccgc
     4261 gcggtggttt ttgggggtcg ggggtgtttg gcagccacag acgcccggtg ttcgtgtcgc
     4321 gccagtacat gcggtccatg cccaggccat ccaaaaacca tgggtctgtc tgctcagtcc
     4381 agtcgtggac cagaccccac gcaacgccca aaataataac ccccacgaac cataaaccat
     4441 tccccatggg ggaccccgtc cctaacccac ggggccagtg gctatggcag ggcctgccgc
     4501 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     4561 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     4621 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acacccgtgc gttttattct
     4681 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     4741 ttagcctccc ccatctcccc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     4801 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     4861 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     4921 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     4981 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     5041 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     5101 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     5161 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     5221 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     5281 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     5341 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     5401 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     5461 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     5521 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
     5581 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     5641 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     5701 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     5761 tcgcggtgag ttcaggcttt ttcatatctc attgcccggg atctgcggca cgctgttgac
     5821 gctgttaagc gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg tcaccttaat
     5881 atgcgaagtg gacctgggac cgcgccgccc cgactgcatc tgcgtgttcg aattcgccaa
     5941 tgacaagacg ctgggcgggg tttgtgtcat catagaacta aagacatgca aatatatttc
     6001 ttccggggac accgccagca aacgcgagca acgggccacg gggatgaagc agggcatggc
     6061 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     6121 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     6181 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     6241 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     6301 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     6361 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     6421 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     6481 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
     6541 cgcagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7021 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     7681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     7981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8341 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8401 ttcgtcttca agaattctca tgtttgacag cttatcatcg cgataagctg atcctcacag
     8461 gccgcaccca gcttttcttc cgttgcccca gtagcatctc tgtctggtga ccttgaagag
     8521 gaagaggagg ggtcccgaga atccccatcc ctaccgtcca gcaaaaaggg ggacgaggaa
     8581 tttgaggcct ggcttgaggc tcaggacgca aatcttgagg atgttcagcg ggagttttcc
     8641 gggctgcgag taattggtga tgaggacgag gatggttcgg aggatgggga attttcagac
     8701 ctggatctgt ctgacagcga ccatgaaggg gatgagggtg ggggggctgt tggagggggc
     8761 aggagtctgc actccctgta ttcactgagc gtcgtctaat aaagatgtct attgatctct
     8821 tttagtgtga atcatgtctg acgaggggcc aggtacagga cctggaaatg gcctaggaga
     8881 gaagggagac acatctggac cagaaggctc cggcggcagt ggacctcaaa gaagaggggg
     8941 tgataaccat ggacgaggac ggggaagagg acgaggacga ggaggcggaa gaccaggagc
     9001 cccgggcggc tcaggatcag ggccaagaca tagagatggt gtccggagac cccaaaaacg
     9061 tccaagttgc attggctgca aagggaccca cggtggaaca ggagcaggag caggagcggg
     9121 aggggcagga gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg
     9181 aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
     9241 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
     9301 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg
     9361 agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc
     9421 aggagcagga ggaggggcag gagcaggagg ggcaggaggg gcaggagggg caggagcagg
     9481 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggggcagg
     9541 agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagggg caggagcagg
     9601 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg
     9661 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg
     9721 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggagg
     9781 ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag gcagtggagg
     9841 ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc gccggggtag
     9901 aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag gtcgtggacg
     9961 tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt ctccaccgcg
    10021 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga
    10081 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca
    10141 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg
    10201 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc
    10261 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag
    10321 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc
    10381 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt
    10441 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga
    10501 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa
    10561 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac
    10621 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg
    10681 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg
    10741 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg
    10801 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat
    10861 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgggcatacc
    10921 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg
    10981 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac
    11041 ctaagaatgg gagcaaccag catgcaggaa aaggacaagc agcgaaaatt cacgccccct
    11101 tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc
    11161 tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac
    11221 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc
    11281 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc
    11341 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc
    11401 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc
    11461 cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc
    11521 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
    11581 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc
    11641 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
    11701 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg
    11761 gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg
    11821 cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct
    11881 atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg
    11941 ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt
    12001 attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca
    12061 atttcaaaaa aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc
    12121 gtttaatttt cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc
    12181 tctttcccct tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct
    12241 gggacacatc ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc
    12301 ataaattcgt gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct
    12361 attgttaaag atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt
    12421 gtgagggtta tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta
    12481 ttctgggggc gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac
    12541 aactcagcag ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag
    12601 gagagttcac tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac
    12661 taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga
    12721 tatcgctgtt ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg
    12781 ggtaacatat gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc
    12841 atatgctacc cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct
    12901 tgagggtccg cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc
    12961 gtagtcttcc tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc
    13021 caagagttac acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca
    13081 ggatgaaagc cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca
    13141 gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt
    13201 ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgaatac aaaacaaaag
    13261 cgctcctcgt accagcgaag aaggggcaga gatgccgtag tcaggtttag ttcgtccggc
    13321 ggcggggc
//

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