pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG载体质粒基本信息

质粒名称: pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG
出品公司: Wekwikgene
目录编号: 0001661
产品用途: 3' AAVLINK plasmid for TCOF1 expression
存储实验室: Zhonghua Lu Lab

载体骨架基本信息
载体名称: pAAV-MCS2
表达系统: 腺病毒
启动子:
SCP1
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称: TCOF1
物种来源: H.sapiens
密码子优化: 未优化

文献引用方法
参考文献部分:
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG载体序列质粒序列多克隆位点信息

pAAVLINKv2-SCP1-Cre-UDeg3a-3-TCOF1-FLAG载体图谱

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG质粒载体简介

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG质粒序列载体序列

LOCUS      pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCOF1-FLAG             6695 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNWKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3408
                     /codon_start=1
                     /label=TCOF1
                     /translation="GAVRRSQTVRRRRRRWLR*SLQGRPTRSELPWLLPRSPPGKGLPQ
                     HLLGRQGLRLPRQGSRMTQGAAARNQTVMGRHRQL*PLPR*LNPL*FLSTLIVVQLAQL
                     LHPHKPRLQAPRGRPEPRRAQPGAPPPRARMRT*SPLHSA*LLASEPMW*PCPLPTQE*
                     PPKPAWLGPAAARSPVGYQMARNRRDQPLRCQRRTQLPSH*PRLP*RSSPRKPVRLSLL
                     LPGPSLQVGLTVLWEHSLQQVPRAPPSRPKGPTSSENLSFLRSSRPPKPLRAQMTVRTA
                     ATVLQGVRKMVKGPRGPSQPTRW*VPPPPGQRPWWRRPQQSPARMMWWRHPSLSSQVI*
                     PLD*PQPIPRPQKPLPS*TPAPQFPLLWPPKMTQMASRRQSPNRQQACCPLKQVEKRLL
                     QAPHLRSPGSPRKGLGTPKPQPWRCKATSPSASWANPGP*MRPRCRPQW*RS*LSCWNR
                     KERRWWTPPRRAAGRAGRAASGSYRETSQLPGPPGARRRRSWGPGKVGRPLFPQKRPPR
                     LPRGKQRETKQVVMSRRRKGRGLLAPKGPRTSQKRSFRRGWGRLKVEIKATQRARRRRR
                     NPTREKKTKKKKKRRRKQKRPQPKILSHRPRRKRRKRRRQQSRL"
     misc_feature    1556..1561
                     /label=SDS
     misc_feature    1574..1579
                     /label=SDS
     misc_feature    1577..1582
                     /label=SDS
     misc_feature    1684..1689
                     /label=SDS
     misc_feature    1931..1936
                     /label=SDS
     misc_feature    2105..2110
                     /label=SDS
     misc_feature    2206..2211
                     /label=SDS
     misc_feature    2678..2683
                     /label=SDS
     misc_feature    2838..2843
                     /label=SDS
     CDS             3415..3480
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3484..3915
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3628..3633
                     /label=SDS
     repeat_region   3958..4098
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3958..4087
                     /label=AAV2 ITR
     rep_origin      4173..4628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4910..5014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5015..5875
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6046..6634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataactgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag cagtgaggag
     1561 gagtcagaca gtgaggagga ggcggagacg ctggctcagg tgaagccttc agggaagacc
     1621 caccagatca gagctgcctt ggctcctgcc aaggagtccc ccaggaaagg ggctgcccca
     1681 acacctcctg ggaagacagg gccttcggct gcccaggcag ggaagcagga tgactcaggg
     1741 agcagcagcg aggaatcaga cagtgatggg gaggcaccgg cagctgtgac ctctgcccag
     1801 gtgattaaac cccctctgat ttttgtcgac cctaatcgta gtccagctgg cccagctgct
     1861 acacccgcac aagcccaggc tgcaagcacc ccgaggaagg cccgagcctc ggagagcaca
     1921 gccaggagct cctcctccga gagcgaggat gaggacgtga tccccgctac acagtgcttg
     1981 actcctggca tcagaaccaa tgtggtgacc atgcccactg cccacccaag aatagccccc
     2041 aaagccagca tggctggggc cagcagcagc aaggagtcca gtcggatatc agatggcaag
     2101 aaacaggagg gaccagccac tcaggtgtca aagaagaacc cagcttccct cccactgacc
     2161 caggctgccc tgaaggtcct cgcccagaaa gccagtgagg ctcagcctcc tgttgccagg
     2221 acccagcctt caagtggggt tgacagtgct gtgggaacac tccctgcaac aagtccccag
     2281 agcacctccg tccaggccaa agggaccaac aagctcagaa aacctaagct tcctgaggtc
     2341 cagcaggcca ccaaagcccc tgagagctca gatgacagtg aggacagcag cgacagttct
     2401 tcagggagtg aggaagatgg tgaagggccc cagggggcca agtcagccca cacgctggta
     2461 ggtcccaccc cctccaggac agagaccctg gtggaggaga ccgcagcaga gtccagcgag
     2521 gatgatgtgg tggcgccatc ccagtctctc ctctcaggtt atatgacccc tggactaacc
     2581 ccagccaatt cccaggcctc aaaagccact cccaagctag actccagccc ctcagtttcc
     2641 tctactctgg ccgccaaaga tgacccagat ggcaagcagg aggcaaagcc ccaacaggca
     2701 gcaggcatgt tgtcccctaa aacaggtgga aaagaggctg cttcaggcac cacacctcag
     2761 aagtcccgga agcccaagaa aggggctggg aacccccaag cctcaaccct ggcgctgcaa
     2821 agcaacatca cccagtgcct cctgggccaa ccctggcccc tgaatgaggc ccaggtgcag
     2881 gcctcagtgg tgaaggtcct gactgagctg ctggaacagg aaagaaagaa ggtggtggac
     2941 accaccaagg agagcagcag gaagggctgg gagagccgca agcggaagct atcgggagac
     3001 cagccagctg ccaggacccc caggagcaag aagaagaaga agctgggggc cggggaaggt
     3061 ggggaggcct ctgtttcccc agaaaagacc tccacgactt ccaaggggaa agcaaagaga
     3121 gacaaagcaa gtggtgatgt caaggagaag aaagggaagg ggtctcttgg ctcccaaggg
     3181 gccaaggacg agccagaaga ggagcttcag aaggggatgg ggacggttga aggtggagat
     3241 caaagcaacc caaagagcaa gaaggagaag aagaaatccg acaagagaaa aaaagacaaa
     3301 gaaaaaaaag aaaagaagaa gaaagcaaaa aaggcctcaa ccaaagattc tgagtcaccg
     3361 tcccagaaga aaaagaagaa aaagaagaag acagcagagc agactgtatg tacagactac
     3421 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     3481 taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3541 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3601 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
     3661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3721 ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3781 tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3841 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3901 tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
     3961 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4021 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4081 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4141 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4381 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4501 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4621 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4921 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4981 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5041 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5101 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5161 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5221 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5281 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5341 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5401 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5461 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5521 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5581 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5641 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5701 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5761 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5821 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5881 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6661 gttcctggcc ttttgctggc cttttgctca catgt
//

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