| 质粒名称: | T7-VEE-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 58970 |
| 存储实验室: | Steven Dowdy |
|
载体骨架基本信息 |
|
| 载体名称: | VEE backbone |
| 载体出品公司: | gift from I. Frolov |
| 空载体大小: | 10793 bp |
| 载体修饰: | 添加了IRES puro |
| 载体类型: | 哺乳动物表达；委内瑞拉马脑炎（VEE）病毒RNA复制子 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 启动子: | 26S subgenomic promoter |
| 3’测序引物 | pCDH-rev GCATTCCTTTGGCGAGAG |
|
文献引用方法 |
|
| 材料和方法部分: | T7-VEE-IRES-Puro was a gift from Steven Dowdy (Addgene plasmid # 58970 ; http://n2t.net/addgene:58970 ; RRID:Addgene_58970) |
| 参考文献部分: | Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS T7-VEE-IRES-Puro 10787 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION noninfectious (non-packaging), self-replicating Venezuelan equine
encephalitis (VEE) virus RNA replicon.
ACCESSION .
VERSION .
KEYWORDS T7-VEE-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10787)
AUTHORS Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR,
Chi NC, Fu XD, Yu BD, Dowdy SF
TITLE Efficient generation of human iPSCs by a synthetic self-replicative
RNA.
JOURNAL Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi:
10.1016/j.stem.2013.06.001.
PUBMED 23910086
REFERENCE 2 (bases 1 to 10787)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10787
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 1535..1543
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
primer_bind complement(7820..7837)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8047..8066
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 8228..8827
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8228..8247)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8724..8744
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
promoter 9025..9129
/gene="bla"
/label=AmpR promoter
CDS 9130..9990
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9348..9367)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10161..10749
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10650..10669
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 10770..10787
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc
421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg
1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct
3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
7561 gtctagcata tgggcgcgcc ctcagcatcg attcaattgg gccggccgcg gccgcaacta
7621 acttaagcta gcaacggttt ccctctagcg ggatcaattc cgcccccccc ccctaacgtt
7681 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
7741 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
7801 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
7861 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
7921 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
7981 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
8041 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
8101 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
8161 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
8221 taataccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
8281 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
8341 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
8401 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
8461 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
8521 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
8581 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
8641 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
8701 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
8761 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
8821 tgcctgagaa ttggcaagct gcttacatag aactcgcggc gattggcatg ccgccttaaa
8881 atttttattt tatttttctt ttcttttccg aatcggattt tgtttttaat atttcaaaaa
8941 aaaaaaaaaa aaaaaaaaaa acgcgtcgag gggaattaat tcttgaagac gaaagggcca
9001 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
9061 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
9121 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
9181 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
9241 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
9301 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
9361 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
9421 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
9481 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
9541 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
9601 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
9661 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
9721 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
9781 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
9841 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
9901 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
9961 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
10021 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
10081 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
10141 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
10201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
10261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
10321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
10381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
10441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
10501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
10561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
10621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
10681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
10741 ctatggaaaa acgccagcaa cgcgagctct aatacgactc actatag
//
