| 出品公司: | Biofeng |
|---|---|
| 载体名称: | pcDNA3.1-Cre |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6647bp |
| 5' 测序引物及序列: | CMV-F: CTAGAGAACCCACTGCTTAC |
| 3' 测序引物及序列: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | G418 |
| 克隆菌株: | DH5a |
| 宿主细胞（系）: | 293T等哺乳细胞系 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 瞬时表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pcDNA3.1-Cre载体质粒基本信息
pcDNA3.1-Cre质粒图谱载体图谱和pcDNA3.1-Cre载体序列质粒序列多克隆位点信息
pcDNA3.1-Cre质粒载体简介
pcDNA3.1-Cre质粒序列载体序列
LOCUS pcDNA3.1-Cre 6647 bp ds-DNA circular SYN 01-DEC-2019
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6647)
AUTHORS 海吉浩格
TITLE Direct Submission
JOURNAL Exported Tuesday, Jun 8, 2021 from SnapGene Viewer 5.1.4.1 to Vector
NTI(R) format
https://www.snapgene.com
COMMENT VNTDATE|801619200|
COMMENT VNTDBDATE|895881600|
COMMENT VNTNAME|pcDNA3.1-Cre|
COMMENT VNTAUTHORNAME|海吉浩格|
FEATURES Location/Qualifiers
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 986..2017
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
promoter complement(2199..2217)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 2243..2467
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2513..2941
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2955..3285
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3136..3271
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3352..4146
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
polyA_signal 4320..4441
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4490..4506)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(4538..4568)
/label=lac promoter
/note="promoter for the E. coli lac operon"
rep_origin complement(4892..5480)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5651..6511)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(6512..6616)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcacg
901 agctggactt taagcaagca cctaaggaca tccaaaagcg taaacggatc cactgttaac
961 ggccgcgggt gtgctggaat tcaccatgtc caatttactg accgtacacc aaaatttgcc
1021 tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag
1081 ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg
1141 ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg
1201 cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt
1261 gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc
1321 tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa
1381 acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag
1441 cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt
1501 acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag
1561 aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc
1621 acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga
1681 tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc
1741 caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat
1801 ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg
1861 tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc
1921 tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaccctgg atagtgaaac
1981 aggggcaatg gtgcgcctgc tggaagatgg cgattagctc gagagtctca tcgccaatga
2041 acgtcaggtg ttttggagaa ggtcacaccg cgcggagatg ttgaaacaaa gatctaactc
2101 ttttgcaaca agatcaataa aataatgtta aaagcaaaaa aaaaaaaaaa aaaaaaaaaa
2161 aaaaatctag atcgatagcg cgctagcccg ggccctattc tatagtgtca cctaaatgct
2221 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
2281 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
2341 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
2401 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
2461 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
2521 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
2581 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
2641 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
2701 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
2761 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
2821 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
2881 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
2941 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc
3001 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3061 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
3241 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
3301 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
3361 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
3421 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
3481 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
3541 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
3601 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
3661 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
3721 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
3781 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
3841 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
3901 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
3961 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
4021 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
4081 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
4141 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
4201 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
4261 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
4321 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4381 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4441 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
4501 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
4561 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4621 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
4681 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
4741 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
4801 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
4861 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
4921 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
4981 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
5041 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
5101 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
5161 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5221 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5281 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
5341 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5401 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
5461 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
5521 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
5581 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
5641 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
5701 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
5761 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
5821 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
5881 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
5941 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
6001 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
6061 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
6121 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6181 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6241 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
6301 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
6361 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
6421 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
6481 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
6541 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
6601 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
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