| 质粒名称: | pJM111 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 134162 |
| 存储实验室: | Iain Cheeseman |
|
载体骨架基本信息 |
|
| 载体名称: | pJAG98 (pBABEblast) |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Nde1_SSSC 295-C |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | SSSC |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | GFP（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJM111 was a gift from Iain Cheeseman (Addgene plasmid # 134162 ; http://n2t.net/addgene:134162 ; RRID:Addgene_134162) |
| 参考文献部分: | Nde1 promotes diverse dynein functions through differential interactions and exhibits an isoform-specific proteasome association. Monda JK, Cheeseman IM. Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 10.1091/mbc.E18-07-0418. Epub 2018 Jul 19. 10.1091/mbc.E18-07-0418 PubMed 30024347 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJM111 6058 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Retroviral plasmid expressing GFP - Nde1.
ACCESSION .
VERSION .
KEYWORDS pJM111
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6058)
AUTHORS Monda JK, Cheeseman IM
TITLE Nde1 promotes diverse dynein functions through differential
interactions and exhibits an isoform-specific proteasome
association.
JOURNAL Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi:
10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
PUBMED 30024347
REFERENCE 2 (bases 1 to 6058)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6058
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 942..1358
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1288..1310
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1326..1342
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 1393..1402
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1399..2115
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1399..2115
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1444..1465)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1705..1724)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2052..2073
/label=EGFP-C
/note="EGFP, forward primer"
CDS 2161..2181
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 2194..2238
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
primer_bind complement(2547..2567)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2552..2881
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2732..2867
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2794..2813
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2889..3287
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
primer_bind complement(4132..4149)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4303..4891)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4383..4402)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5070..5930)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5693..5712
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5931..6035)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
1381 acgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
1441 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
1501 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
1561 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
1621 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
1681 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
1741 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
1801 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
1861 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
1921 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
1981 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
2041 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
2101 gacgagctgt acaagtccgg aggggatcgg tggagctcca ccggtggcgg ccgctctaga
2161 gagaatcttt attttcaggg cgccgccaaa ttcaaagaaa ccgctgctgc taaattcgaa
2221 cgccagcaca tggacagcgg aggtggaggt actagtctcg agatgagcaa gaacagagat
2281 ggcggggaga gacggccaag cagcaccagc gtgcctttgg gtgataaggg gttggacacg
2341 agttgccgct ggttgtccaa atcaacaacc aggtcgtcca gctcgtgctg accgcgggcc
2401 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
2461 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
2521 ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
2581 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
2641 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2701 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
2761 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
2821 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
2881 aaagcttcat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
2941 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
3001 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
3061 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
3121 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
3181 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
3241 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagat cgataaaata
3301 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
3361 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
3421 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
3481 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
3541 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
3601 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
3661 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
3721 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
3781 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
3841 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
3901 wmtmcmmkwm ascskskkkc wkttgcatcc gacttgtggt ctcgctgttc cttgggaggg
3961 tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa
4021 attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat
4081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
4141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
4321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
4501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4741 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
4861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
4921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
4981 gatcttcacc tagatccttt tgcggccgca aatcaatcta aagtatatat gwswawmyww
5041 rgtmtrwmwg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
5101 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
5161 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
5221 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
5281 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
5341 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
5401 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
5461 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
5521 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
5581 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
5641 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
5701 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
5761 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
5821 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
5881 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
5941 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
6001 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
