pShuttle-CMV-EGFP-C

价格:1600元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pShuttle-CMV-EGFP-C
质粒类型: 腺病毒载体
高拷贝/低拷贝: 低拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8156 bp
5' 测序引物及序列: GGCACCAAAATCAACGGGACTTTCC
3' 测序引物及序列: --
载体标签: EGFP
载体抗性: Kanamycin
筛选标记: --
备注: 克隆菌株:DH5alpha
产品目录号: 13887
稳定性: --
组成型: --
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pShuttle-CMV-EGFP-C载体图谱



pShuttle-CMV-EGFP-C载体图谱2



载体简介

载体序列

LOCUS       pShuttle-CMV-EGFP-C	8156 bp 	DNA	SYN
DEFINITION  pShuttle-CMV-EGFP-C
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="pShuttle-CMV-EGFP-C"
                     /mol_type="other DNA"
     misc_feature    complement(27..105)
                     /label="RITR"
     misc_feature    27..105
                     /label="LITR"
     misc_feature    185..333
                     /label="Encap"
     promoter        349..901
                     /label="CMV_immearly_promoter"
     misc_feature    404..691
                     /label="CAG_enhancer"
     misc_feature    858..878
                     /label="CMV_fwd_primer"
     promoter        859..928
                     /label="CMV_promoter"
     CDS             972..1763
                     /label="ORF frame 3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
     gene            975..1688
                     /label="EGFP"
                     /gene="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
     misc_feature    complement(1017..1038)
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
     misc_feature    1155..1184
                     /label="Y66 (EGFP)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
     misc_feature    1625..1646
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
     terminator      1805..2033
                     /label="SV40_PA_terminator"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
     misc_feature    2003..2022
                     /label="EBV_rev_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
     CDS             complement(2609..3967)
                     /label="ORF frame 2"
                     /translation="MLPGRRAGRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADE
                     DPAPVERHDPGPSGRAPTTAVQRKPPQPAKRGDMLDRDAVEQVTELWDRLELLGQTLK
                     SMPTADGLKPLKNFASLQELLSLGGERLLADLVRENMRVRDMLNEVAPLLRDDGSCSS
                     LNYQLHPVIGVIYGPTGCGKSQLLRNLLSSQLISPTPETVFFIAPQVDMIPPSELKAW
                     EMQICEGNYAPGPDGTIIPQSGTLRPRFVKMAYDDLILEHNYDVSDPRNIFAQAAARG
                     PIAIIMDECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDMAGN
                     IANLKIQSKMHLISPRMHPSQLNRFVNTYTKGLPLAISLLLKDIFRHHAQRSCYDWII
                     YNTTPQHEALQWCYLHPRDGLMPMYLNIQSHLYHVLEKIHRTLNDRDRWSRAYRARKT
                     PK*"
     CDS             complement(3715..4236)
                     /label="ORF frame 3"
                     /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
                     PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
                     RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
                     NPRNEEICWIEMP*"
     misc_feature    complement(5194..5289)
                     /label="LITR"
                     /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
                     PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
                     RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
                     NPRNEEICWIEMP*"
     misc_feature    5194..5289
                     /label="RITR"
                     /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
                     PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
                     RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
                     NPRNEEICWIEMP*"
     rep_origin      complement(5534..6153)
                     /label="pBR322_origin"
                     /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
                     PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
                     RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
                     NPRNEEICWIEMP*"
     gene            complement(6308..6607)
                     /label="Ampicillin (299 - 0)"
                     /gene="Ampicillin (299 - 0)"
                     /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
                     PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
                     RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
                     NPRNEEICWIEMP*"
     promoter        6810..6859
                     /label="NEOKAN_promoter"
                     /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
                     PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
                     RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
                     NPRNEEICWIEMP*"
     CDS             6948..7742
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6951..7739
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
ORIGIN
    1 NNTCCCTTCC AGCTCTCTGC CCCTTTTGGA TTGAAGCCAA TATGATAATG AGGGGGTGGA
   61 GTTTGTGACG TGGCGCGGGG CGTGGGAACG GGGCGGGTGA CGTAGTAGTG TGGCGGAAGT
  121 GTGATGTTGC AAGTGTGGCG GAACACATGT AAGCGACGGA TGTGGCAAAA GTGACGTTTT
  181 TGGTGTGCGC CGGTGTACAC AGGAAGTGAC AATTTTCGCG CGGTTTTAGG CGGATGTTGT
  241 AGTAAATTTG GGCGTAACCG AGTAAGATTT GGCCATTTTC GCGGGAAAAC TGAATAAGAG
  301 GAAGTGAAAT CTGAATAATT TTGTGTTACT CATAGCGCGT AANNNNTAAT AGTAATCAAT
  361 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA
  421 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT
  481 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA
  541 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT
  601 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC
  661 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA
  721 GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT
  781 TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA
  841 CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
  901 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGAGATCg atcTGGTACC GTCGACGTCG
  961 CGGCCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG
 1021 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG
 1081 ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC
 1141 CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG
 1201 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC
 1261 GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG
 1321 GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA
 1381 TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA
 1441 AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG
 1501 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
 1561 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG
 1621 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC
 1681 TGTACAAGTC CGGACTCAGA TCTGTAGGTG CATTAATGGA AGTGATGCAG AAGAGGAGCA
 1741 AAGCCATTCA TTCTTCAGAC TGAAGCTTCT AGATAAGATA TCCGATCCAC CGGATCTAGA
 1801 TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC
 1861 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT
 1921 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
 1981 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGGA
 2041 Tctgggcgtg gtTAAGGGTG GGAAAGAATA TATAAGGTGG GGGTCTTATG TAGTTTTGTA
 2101 TCTGTTTTGC AGCAGCCGCC GCCGCCATGA GCACCAACTC GTTTGATGGA AGCATTGTGA
 2161 GCTCATATTT GACAACGCGC ATGCCCCCAT GGGCCGGGGT GCGTCAGAAT GTGATGGGCT
 2221 CCAGCATTGA TGGTCGCCCC GTCCTGCCCG CAAACTCTAC TACCTTGACC TACGAGACCG
 2281 TGTCTGGAAC GCCGTTGGAG ACTGCAGCCT CCGCCGCCGC TTCAGCCGCT GCAGCCACCG
 2341 CCCGCGGGAT TGTGACTGAC TTTGCTTTCC TGAGCCCGCT TGCAAGCAGT GCAGCTTCCC
 2401 GTTCATCCGC CCGCGATGAC AAGTTGACGG CTCTTTTGGC ACAATTGGAT TCTTTGACCC
 2461 GGGAACTTAA TGTCGTTTCT CAGCAGCTGT TGGATCTGCG CCAGCAGGTT TCTGCCCTGA
 2521 AGGCTTCCTC CCCTCCCAAT GCGGTTTAAA ACATAAATAA AAAACCAGAC TCTGTTTGGA
 2581 TTTGGATCAA GCAAGTGTCT TGCTGTCTTT ATTTAGGGGT TTTGCGCGCG CGGTAGGCCC
 2641 GGGACCAGCG GTCTCGGTCG TTGAGGGTCC TGTGTATTTT TTCCAGGACG TGGTAAAGGT
 2701 GACTCTGGAT GTTCAGATAC ATGGGCATAA GCCCGTCTCT GGGGTGGAGG TAGCACCACT
 2761 GCAGAGCTTC ATGCTGCGGG GTGGTGTTGT AGATGATCCA GTCGTAGCAG GAGCGCTGGG
 2821 CGTGGTGCCT AAAAATGTCT TTCAGTAGCA AGCTGATTGC CAGGGGCAGG CCCTTGGTGT
 2881 AAGTGTTTAC AAAGCGGTTA AGCTGGGATG GGTGCATACG TGGGGATATG AGATGCATCT
 2941 TGGACTGTAT TTTTAGGTTG GCTATGTTCC CAGCCATATC CCTCCGGGGA TTCATGTTGT
 3001 GCAGAACCAC CAGCACAGTG TATCCGGTGC ACTTGGGAAA TTTGTCATGT AGCTTAGAAG
 3061 GAAATGCGTG GAAGAACTTG GAGACGCCCT TGTGACCTCC AAGATTTTCC ATGCATTCGT
 3121 CCATAATGAT GGCAATGGGC CCACGGGCGG CGGCCTGGGC GAAGATATTT CTGGGATCAC
 3181 TAACGTCATA GTTGTGTTCC AGGATGAGAT CGTCATAGGC CATTTTTACA AAGCGCGGGC
 3241 GGAGGGTGCC AGACTGCGGT ATAATGGTTC CATCCGGCCC AGGGGCGTAG TTACCCTCAC
 3301 AGATTTGCAT TTCCCACGCT TTGAGTTCAG ATGGGGGGAT CATGTCTACC TGCGGGGCGA
 3361 TGAAGAAAAC GGTTTCCGGG GTAGGGGAGA TCAGCTGGGA AGAAAGCAGG TTCCTGAGCA
 3421 GCTGCGACTT ACCGCAGCCG GTGGGCCCGT AAATCACACC TATTACCGGG TGCAACTGGT
 3481 AGTTAAGAGA GCTGCAGCTG CCGTCATCCC TGAGCAGGGG GGCCACTTCG TTAAGCATGT
 3541 CCCTGACTCG CATGTTTTCC CTGACCAAAT CCGCCAGAAG GCGCTCGCCG CCCAGCGATA
 3601 GCAGTTCTTG CAAGGAAGCA AAGTTTTTCA ACGGTTTGAG ACCGTCCGCC GTAGGCATGC
 3661 TTTTGAGCGT TTGACCAAGC AGTTCCAGGC GGTCCCACAG CTCGGTCACC TGCTCTACGG
 3721 CATCTCGATC CAGCATATCT CCTCGTTTCG CGGGTTGGGG CGGCTTTCGC TGTACGGCAG
 3781 TAGTCGGTGC TCGTCCAGAC GGGCCAGGGT CATGTCTTTC CACGGGCGCA GGGTCCTCGT
 3841 CAGCGTAGTC TGGGTCACGG TGAAGGGGTG CGCTCCGGGC TGCGCGCTGG CCAGGGTGCG
 3901 CTTGAGGCTG GTCCTGCTGG TGCTGAAGCG CTGCCGGTCT TCGCCCTGCG CGTCGGCCAG
 3961 GTAGCATTTG ACCATGGTGT CATAGTCCAG CCCCTCCGCG GCGTGGCCCT TGGCGCGCAG
 4021 CTTGCCCTTG GAGGAGGCGC CGCACGAGGG GCAGTGCAGA CTTTTGAGGG CGTAGAGCTT
 4081 GGGCGCGAGA AATACCGATT CCGGGGAGTA GGCATCCGCG CCGCAGGCCC CGCAGACGGT
 4141 CTCGCATTCC ACGAGCCAGG TGAGCTCTGG CCGTTCGGGG TCAAAAACCA GGTTTCCCCC
 4201 ATGCTTTTTG ATGCGTTTCT TACCTCTGGT TTCCATGAGC CGGTGTCCAC GCTCGGTGAC
 4261 GAAAAGGCTG TCCGTGTCCC CGTATACAGA CTNNNGTTTA AACGAATTCN NNATATAAAA
 4321 TGCAAGGTGC TGCTCAAAAA ATCAGGCAAA GCCTCGCGCA AAAAAGAAAG CACATCGTAG
 4381 TCATGCTCAT GCAGATAAAG GCAGGTAAGC TCCGGAACCA CCACAGAAAA AGACACCATT
 4441 TTTCTCTCAA ACATGTCTGC GGGTTTCTGC ATAAACACAA AATAAAATAA CAAAAAAACA
 4501 TTTAAACATT AGAAGCCTGT CTTACAACAG GAAAAACAAC CCTTATAAGC ATAAGACGGA
 4561 CTACGGCCAT GCCGGCGTGA CCGTAAAAAA ACTGGTCACC GTGATTAAAA AGCACCACCG
 4621 ACAGCTCCTC GGTCATGTCC GGAGTCATAA TGTAAGACTC GGTAAACACA TCAGGTTGAT
 4681 TCATCGGTCA GTGCTAAAAA GCGACCGAAA TAGCCCGGGG GAATACATAC CCGCAGGCGT
 4741 AGAGACAACA TTACAGCCCC CATAGGAGGT ATAACAAAAT TAATAGGAGA GAAAAACACA
 4801 TAAACACCTG AAAAACCCTC CTGCCTAGGC AAAATAGCAC CCTCCCGCTC CAGAACAACA
 4861 TACAGCGCTT CACAGCGGCA GCCTAACAGT CAGCCTTACC AGTAAAAAAG AAAACCTATT
 4921 AAAAAAACAC CACTCGACAC GGCACCAGCT CAATCAGTCA CAGTGTAAAA AAGGGCCAAG
 4981 TGCAGAGCGA GTATATATAG GACTAAAAAA TGACGTAACG GTTAAAGTCC ACAAAAAACA
 5041 CCCAGAAAAC CGCACGCGAA CCTACGCCCA GAAACGAAAG CCAAAAAACC CACAACTTCC
 5101 TCAAATCGTC ACTTCCGTTT TCCCACGTTA CGTAACTTCC CATTTTAAGA AAACTACAAT
 5161 TCCCAACACA TACAAGTTAC TCCGCCCTAA AACCTACGTC ACCCGCCCCG TTCCCACGCC
 5221 CCGCGCCACG TCACAAACTC CACCCCCTCA TTATCATATT GGCTTCAATC CAAAATAAGG
 5281 TATATTATTG ATGATNNNTT AATTAAGGAT CCNNNCGGTG TGAAATACCG CACAGATGCG
 5341 TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
 5401 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
 5461 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
 5521 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
 5581 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
 5641 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
 5701 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
 5761 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
 5821 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
 5881 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
 5941 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG
 6001 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
 6061 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
 6121 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
 6181 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
 6241 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
 6301 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
 6361 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
 6421 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG
 6481 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
 6541 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
 6601 TGCGCAACGT TGTTGNNNNA AAAAGGATCT TCACCTAGAT CCTTTTCACG TAGAAAGCCA
 6661 GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG
 6721 AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG
 6781 ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA
 6841 AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTC GCCGCCAAGG ATCTGATGGC
 6901 GCAGGGGATC AAGCTCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG
 6961 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG
 7021 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC
 7081 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG
 7141 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA
 7201 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT
 7261 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA
 7321 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC
 7381 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC
 7441 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG
 7501 TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG
 7561 GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
 7621 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG
 7681 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT
 7741 GAATTTTGTT AAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC
 7801 AACATCCCTT ATAAATCAAA AGAATAGACC GCGATAGGGT TGAGTGTTGT TCCAGTTTGG
 7861 AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT
 7921 CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA GTTTTTTGCG GTCGAGGTGC
 7981 CGTAAAGCTC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG
 8041 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG
 8101 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CGCGCTTAAT GCGCCG

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