FUW-tetO-hMYC

价格:900元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: FUW-tetO-hMYC
质粒类型: 慢病毒系统载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9786bp
5' 测序引物及序列: LNCX: AGCTCGTTTAGTGAACCGTCAGATC
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: 博来霉素(Zeocin)
备注: 克隆菌株为Stbl3和TOP10。
产品目录号: 20723
稳定性: --
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

FUW-tetO-hMYC载体图谱



载体简介

载体序列

LOCUS       FUW-tetO-hMYC	9786 bp 	DNA	SYN
DEFINITION  FUW-tetO-hMYC
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="FUW-tetO-hMYC"
                     /mol_type="other DNA"
     promoter        8..584
                     /label="CMV_immearly_promoter"
     misc_feature    87..374
                     /label="CAG_enhancer"
     misc_feature    541..561
                     /label="CMV_fwd_primer"
     misc_feature    604..784
                     /label="truncHIV-1_3_LTR"
     misc_feature    604..784
                     /label="HIV-1_5_LTR"
     misc_feature    895..939
                     /label="HIV-1_psi_pack"
     CDS             1327..2214
                     /label="ORF frame 1"

     misc_feature    1449..1682
                     /label="RRE"

     misc_feature    2213..2228
                     /label="cPPT"
  
     misc_feature    2371..2688
                     /label="TRE"

     promoter        2689..2808
                     /label="CMV2_promoter"
 
     misc_feature    2720..2739
                     /label="pCEP_fwd_primer"

     misc_feature    2722..2746
                     /label="LNCX_primer"

     CDS             2903..4222
                     /label="ORF frame 2"

     misc_feature    4130..4159
                     /label="Myc"

     misc_feature    4249..4836
                     /label="WPRE"

     CDS             4337..5386
                     /label="ORF frame 2"

     CDS             4350..4862
                     /label="ORF frame 3"

     misc_feature    complement(4839..4855)
                     /label="pBluescriptKS_primer"

     misc_feature    5023..5044
                     /label="U3PPT"

     misc_feature    5023..5038
                     /label="cPPT"

     misc_feature    5360..5540
                     /label="truncHIV-1_3_LTR"

     misc_feature    5360..5540
                     /label="HIV-1_5_LTR"

     misc_feature    complement(5566..5583)
                     /label="BGH_rev_primer"

     terminator      5569..5796
                     /label="bGH_PA_terminator"

     rep_origin      5859..6165
                     /label="f1_origin"

     misc_feature    complement(6279..6299)
                     /label="pBABE_3_primer"

     misc_feature    complement(6285..6500)
                     /label="SV40_enhancer"

     promoter        6297..6565
                     /label="SV40_promoter"

     rep_origin      6464..6541
                     /label="SV40_origin"

     misc_feature    6526..6545
                     /label="SV40pro_F_primer"

     promoter        6659..6726
                     /label="EM7_promoter"

     gene            6727..7098
                     /label="bleo"
                     /gene="bleo"

     misc_feature    6727..7101
                     /label="sh_ble"

     terminator      7234..7353
                     /label="SV40_PA_terminator"

     misc_feature    7322..7341
                     /label="EBV_rev_primer"

     promoter        complement(7397..7415)
                     /label="M13_reverse_primer"

     misc_feature    complement(7414..7436)
                     /label="M13_pUC_rev_primer"

     promoter        complement(7450..7479)
                     /label="lac_promoter"

     gene            complement(8562..9422)
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             complement(8562..9422)
                     /label="ORF frame 2"

     promoter        complement(9464..9492)
                     /label="AmpR_promoter"

ORIGIN
    1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
   61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
  121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
  181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
  241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
  301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
  361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
  421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
  481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
  541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
  601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
  661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
  721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
  781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
  841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
  901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
  961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
 1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
 1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
 1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
 1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
 1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
 1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
 1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
 1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
 1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
 1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
 1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
 1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
 1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
 1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
 1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
 1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
 1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
 2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
 2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
 2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
 2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
 2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
 2341 ggacagcaga gatccagttt ggttaatgat ctcgagttta ccactcccta tcagtgatag
 2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
 2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
 2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
 2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
 2641 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgagta ggcgtgtacg
 2701 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
 2761 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
 2821 attcctctga aaggctctcc ttgcagctgc ttagacgctg gatttttttc gggtagtgga
 2881 aaaccagcag cctcccgcga cgatgcccct caacgttagc ttcaccaaca ggaactatga
 2941 cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga acttctacca
 3001 gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct ggaagaaatt
 3061 cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct gctcgccctc
 3121 ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg gcgggagctt
 3181 ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca tggtgaacca
 3241 gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca tccaggactg
 3301 tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg cctcctacca
 3361 ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg tctgctccac
 3421 ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg acccctcggt
 3481 ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct cgcaagactc
 3541 cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct ccccgcaggg
 3601 cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca gcgactctga
 3661 ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga ggcaggctcc
 3721 tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac ctcctcacag
 3781 cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg cagcgcctcc
 3841 ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg tcagagtcct
 3901 gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca ccgaggagaa
 3961 tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag
 4021 cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt
 4081 agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct
 4141 catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca
 4201 gctacggaac tcttgtgcgt aagaattcga tatcaagctt atcgataatc aacctctgga
 4261 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
 4321 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
 4381 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
 4441 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
 4501 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
 4561 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
 4621 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
 4681 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
 4741 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
 4801 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
 4861 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
 4921 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
 4981 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
 5041 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
 5101 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
 5161 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
 5221 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
 5281 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
 5341 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
 5401 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
 5461 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
 5521 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
 5581 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
 5641 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
 5701 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
 5761 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
 5821 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
 5881 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
 5941 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
 6001 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
 6061 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
 6121 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
 6181 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
 6241 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
 6301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
 6361 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
 6421 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
 6481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
 6541 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
 6601 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
 6661 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
 6721 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
 6781 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
 6841 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
 6901 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
 6961 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
 7021 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
 7081 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
 7141 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
 7201 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
 7261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
 7321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
 7381 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
 7441 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
 7501 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
 7561 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
 7621 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
 7681 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
 7741 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
 7801 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
 7861 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
 7921 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
 7981 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
 8041 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
 8101 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
 8161 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
 8221 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
 8281 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
 8341 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
 8401 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
 8461 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
 8521 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
 8581 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
 8641 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
 8701 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
 8761 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
 8821 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
 8881 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
 8941 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
 9001 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
 9061 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
 9121 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
 9181 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
 9241 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
 9301 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
 9361 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
 9421 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
 9481 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
 9541 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
 9601 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
 9661 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
 9721 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
 9781 gatata
//

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