| 出品公司: | -- |
|---|---|
| 载体名称: | pKD13 |
| 质粒类型: | 细菌表达；基因敲除系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3434 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | -- |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pKD13 3434 bp DNA circular SYN 11-SEP-2001
DEFINITION Template plasmid pKD13, complete sequence.
ACCESSION AY048744
VERSION AY048744.1 GI:15554335
KEYWORDS .
SOURCE Template plasmid pKD13
ORGANISM Template plasmid pKD13
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3434)
AUTHORS Datsenko,K.A. and Wanner,B.L.
TITLE One-step inactivation of chromosomal genes in Escherichia coli K-12
using PCR products
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000)
PUBMED 10829079
REFERENCE 2 (bases 1 to 3434)
AUTHORS Datsenko,K.A. and Wanner,B.L.
TITLE Direct Submission
JOURNAL Submitted (29-JUL-2001) Biological Sciences, Purdue University,
Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA
FEATURES Location/Qualifiers
source 1..3434
/organism="Template plasmid pKD13"
/mol_type="genomic DNA"
/db_xref="taxon:170493"
misc_feature 31..50
/note="priming site 1"
misc_feature complement(51..98)
/note="natural FRT site"
CDS complement(111..905)
/note="kanamycin resistance"
/codon_start=1
/transl_table=11
/product="Tn5 neomycin phosphotransferase"
/protein_id="AAL02037.1"
/db_xref="GI:15554336"
misc_feature 274..293
/note="common priming site kt"
misc_feature 744..763
/note="common priming site k2"
misc_feature 835..854
/note="common priming site k1"
misc_feature complement(1273..1307)
/note="distal 35-nt of natural FRT site"
misc_feature 1334..1353
/note="priming site 4"
terminator 1402..1644
/note="lambda terminator"
rep_origin 1678..2076
/note="R6K gamma replication origin"
CDS complement(2146..3006)
/note="bla; ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAL02038.1"
/db_xref="GI:15554337"
terminator 3153..3412
/note="rrnB transcription terminator"
ORIGIN
1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctta ttagaagaac
121 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
181 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
241 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
301 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
361 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
421 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
481 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
541 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
601 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
661 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
721 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
781 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
841 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
901 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
961 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
1021 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
1081 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
1141 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
1201 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgagctt
1261 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcgaac tgcaggtcga
1321 cggatccccg gaattaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc
1381 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt
1441 acaaagcgag gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag
1501 cggctggctg aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg
1561 agttaagaac gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc
1621 caataccagt agaaacagac gaagaagcta gctttgcact ggattgcgag gctttgccat
1681 ggctaattcc catgtcagcc gttaagtgtt cctgtgtcac tgaaaattgc tttgagaggc
1741 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa
1801 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta
1861 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac
1921 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg
1981 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt
2041 agtacgtact atcaacaggt tgaactgcgg atcttgcggc cgcaaaaatt aaaaatgaag
2101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
2341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
2401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2461 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2761 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
2941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3121 ccgaaaagtg ccacctgcat cgatggcccc ccgatggtag tgtggggtct ccccatgcga
3181 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
3241 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
3301 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
3361 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtggccagtg
3421 ccaagcttgc atgc
///
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