pFGC-pcoCas9

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pFGC-pcoCas9
质粒类型: 植物载体;RNAi载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: 35SPPDK promoter
载体大小: 13330 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 2XFLAG、SV40NLS(N ter)
载体抗性: 卡那霉素
筛选标记: 草胺膦、草丁膦
克隆菌株: HB101等菌株
宿主细胞(系): 农杆菌/植物细胞
备注: --
产品目录号: #52256
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

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载体质粒图谱和多克隆位点信息

pFGC-pcoCas9载体图谱



载体简介

载体序列

LOCUS       Exported               13330 bp ds-DNA     circular SYN 27-JUL-2016
DEFINITION  Binary plant vector for expressing plant codon-optimized Case9 from 
            the 35SPPDK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pFGC-pcoCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Sheen Lab / Addgene #52256
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, July 27, 2016
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pFGC-pcoCas9|
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     misc_feature    5..145
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      489..683
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(749..1822)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2251..2880)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4181..4205
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        4487..4996
                     /note="35SPPDK promoter"
                     /note="hybrid promoter consisting of the cauliflower mosaic
                     virus 35S enhancer fused to the maize C4PPDK basal promoter
                     (Yoo et al., 2007)"
     CDS             5005..5007
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             5008..5055
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /note="2xFLAG"
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             5062..5082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             join(5107..6543,6733..9396)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /note="pcoCas9"
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          6544..6732
                     /note="IV2 intron"
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             9397..9444
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     terminator      9453..9705
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9946..10326
                     /note="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             10332..10883
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="BlpR"
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      10893..11145
                     /note="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    11231..11255
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11680..12474
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12561..13149
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
      301 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
      361 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
      421 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
      481 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
      541 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
      601 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
      661 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
      721 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
      781 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
      841 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
      901 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
      961 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     1021 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     1081 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     1141 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     1201 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     1261 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     1321 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     1381 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     1441 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     1501 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     1561 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     1621 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     1681 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     1741 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     1801 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     1861 gggccggcag gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     1921 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     1981 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     2041 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     2101 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     2161 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     2221 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     2281 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     2341 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     2401 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     2461 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     2521 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     2581 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     2641 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
     2701 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
     2761 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
     2821 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
     2881 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
     2941 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
     3001 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
     3061 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
     3121 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
     3181 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
     3241 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
     3301 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
     3361 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
     3421 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
     3481 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
     3541 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
     3601 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
     3661 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
     3721 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
     3781 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
     3841 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
     3901 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
     3961 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
     4021 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
     4081 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt
     4141 taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
     4201 tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc
     4261 tgctctagca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     4321 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     4381 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttaggcctt
     4441 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     4501 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     4561 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     4621 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacaagga
     4681 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     4741 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgccccaa gcttgggccc
     4801 aagcttgggt cgcgccccac ggatggtata agaataaagg cattccgcgt gcaggattca
     4861 cccgttcgcc tctcaccttt tcgctgtact ctctcgccac acacaccccc tctccagctc
     4921 cgttggagct ccggacagca gcaggcgcgg ggcggtcacg tagtaagcag ctctcggctc
     4981 cctctcccct tgctccgtgg atccatggat tacaaggatg atgatgataa ggattacaag
     5041 gatgatgatg ataagatggc tccaaagaag aagagaaagg ttggaatcca cggagttcca
     5101 gctgctgata agaagtactc tatcggactt gacatcggaa ccaactctgt tggatgggct
     5161 gttatcaccg atgagtacaa ggttccatct aagaagttca aggttcttgg aaacaccgat
     5221 agacactcta tcaagaagaa ccttatcggt gctcttcttt tcgattctgg agagaccgct
     5281 gaggctacca gattgaagag aaccgctaga agaagataca ccagaagaaa gaacagaatc
     5341 tgctaccttc aggaaatctt ctctaacgag atggctaagg ttgatgattc tttcttccac
     5401 agacttgagg agtctttcct tgttgaggag gataagaagc acgagagaca cccaatcttc
     5461 ggaaacatcg ttgatgaggt tgcttaccac gagaagtacc caaccatcta ccaccttaga
     5521 aagaagttgg ttgattctac cgataaggct gatcttagac ttatctacct tgctcttgct
     5581 cacatgatca agttcagagg acacttcctt atcgagggag accttaaccc agataactct
     5641 gatgttgata agttgttcat ccagcttgtt cagacctaca accagctttt cgaggagaac
     5701 ccaatcaacg cttctggagt tgatgctaag gctatccttt ctgctagact ttctaagtct
     5761 cgtagacttg agaaccttat cgctcagctt ccaggagaga agaagaacgg acttttcgga
     5821 aaccttatcg ctctttctct tggacttacc ccaaacttca agtctaactt cgatcttgct
     5881 gaggatgcta agttgcagct ttctaaggat acctacgatg atgatcttga taaccttctt
     5941 gctcagatcg gagatcagta cgctgatctt ttccttgctg ctaagaacct ttctgatgct
     6001 atccttcttt ctgacatcct tagagttaac accgagatca ccaaggctcc actttctgct
     6061 tctatgatca agagatacga tgagcaccac caggatctta cccttttgaa ggctcttgtt
     6121 agacagcagc ttccagagaa gtacaaggaa atcttcttcg atcagtctaa gaacggatac
     6181 gctggataca tcgatggagg agcttctcag gaggagttct acaagttcat caagccaatc
     6241 cttgagaaga tggatggaac cgaggagctt cttgttaagt tgaacagaga ggatcttctt
     6301 agaaagcaga gaaccttcga taacggatct atcccacacc agatccacct tggagagctt
     6361 cacgctatcc ttcgtagaca ggaggatttc tacccattct tgaaggataa cagagagaag
     6421 atcgagaaga tccttacctt cagaatccca tactacgttg gaccacttgc tagaggaaac
     6481 tctcgtttcg cttggatgac cagaaagtct gaggagacca tcaccccttg gaacttcgag
     6541 gaggtaagtt tctgcttcta cctttgatat atatataata attatcatta attagtagta
     6601 atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt
     6661 ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt
     6721 gttgatgtgc aggttgttga taagggagct tctgctcagt ctttcatcga gagaatgacc
     6781 aacttcgata agaaccttcc aaacgagaag gttcttccaa agcactctct tctttacgag
     6841 tacttcaccg tttacaacga gcttaccaag gttaagtacg ttaccgaggg aatgagaaag
     6901 ccagctttcc tttctggaga gcagaagaag gctatcgttg atcttctttt caagaccaac
     6961 agaaaggtta ccgttaagca gttgaaggag gattacttca agaagatcga gtgcttcgat
     7021 tctgttgaaa tctctggagt tgaggataga ttcaacgctt ctcttggaac ctaccacgat
     7081 cttttgaaga tcatcaagga taaggatttc cttgataacg aggagaacga ggacatcctt
     7141 gaggacatcg ttcttaccct tacccttttc gaggatagag agatgatcga ggagagactc
     7201 aagacctacg ctcacctttt cgatgataag gttatgaagc agttgaagag aagaagatac
     7261 accggatggg gtagactttc tcgtaagttg atcaacggaa tcagagataa gcagtctgga
     7321 aagaccatcc ttgatttctt gaagtctgat ggattcgcta acagaaactt catgcagctt
     7381 atccacgatg attctcttac cttcaaggag gacatccaga aggctcaggt ttctggacag
     7441 ggagattctc ttcacgagca catcgctaac cttgctggat ctccagctat caagaaggga
     7501 atccttcaga ccgttaaggt tgttgatgag cttgttaagg ttatgggtag acacaagcca
     7561 gagaacatcg ttatcgagat ggctagagag aaccagacca cccagaaggg acagaagaac
     7621 tctcgtgaga gaatgaagag aatcgaggag ggaatcaagg agcttggatc tcaaatcttg
     7681 aaggagcacc cagttgagaa cacccagctt cagaacgaga agttgtacct ttactacctt
     7741 cagaacggaa gagatatgta cgttgatcag gagcttgaca tcaacagact ttctgattac
     7801 gatgttgatc acatcgttcc acagtctttc ttgaaggatg attctatcga taacaaggtt
     7861 cttacccgtt ctgataagaa cagaggaaag tctgataacg ttccatctga ggaggttgtt
     7921 aagaagatga agaactactg gagacagctt cttaacgcta agttgatcac ccagagaaag
     7981 ttcgataacc ttaccaaggc tgagagagga ggactttctg agcttgataa ggctggattc
     8041 atcaagagac agcttgttga gaccagacag atcaccaagc acgttgctca gatccttgat
     8101 tctcgtatga acaccaagta cgatgagaac gataagttga tcagagaggt taaggttatc
     8161 accttgaagt ctaagttggt ttctgatttc agaaaggatt tccagttcta caaggttaga
     8221 gagatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt tggaaccgct
     8281 cttatcaaga agtacccaaa gttggagtct gagttcgttt acggagatta caaggtttac
     8341 gatgttagaa agatgatcgc taagtctgag caggagatcg gaaaggctac cgctaagtac
     8401 ttcttctact ctaacatcat gaacttcttc aagaccgaga tcacccttgc taacggagag
     8461 atcagaaaga gaccacttat cgagaccaac ggagagaccg gagagatcgt ttgggataag
     8521 ggaagagatt tcgctaccgt tagaaaggtt ctttctatgc cacaggttaa catcgttaag
     8581 aaaaccgagg ttcagaccgg aggattctct aaggagtcta tccttccaaa gagaaactct
     8641 gataagttga tcgctagaaa gaaggattgg gacccaaaga agtacggagg attcgattct
     8701 ccaaccgttg cttactctgt tcttgttgtt gctaaggttg agaagggaaa gtctaagaag
     8761 ttgaagtctg ttaaggagct tcttggaatc accatcatgg agcgttcttc tttcgagaag
     8821 aacccaatcg atttccttga ggctaaggga tacaaggagg ttaagaagga tcttatcatc
     8881 aagttgccaa agtactctct tttcgagctt gagaacggaa gaaagagaat gcttgcttct
     8941 gctggagagc ttcagaaggg aaacgagctt gctcttccat ctaagtacgt taacttcctt
     9001 taccttgctt ctcactacga gaagttgaag ggatctccag aggataacga gcagaagcag
     9061 cttttcgttg agcagcacaa gcactacctt gatgagatca tcgagcaaat ctctgagttc
     9121 tctaagagag ttatccttgc tgatgctaac cttgataagg ttctttctgc ttacaacaag
     9181 cacagagata agccaatcag agagcaggct gagaacatca tccacctttt cacccttacc
     9241 aaccttggtg ctccagctgc tttcaagtac ttcgatacca ccatcgatag aaaaagatac
     9301 acctctacca aggaggttct tgatgctacc cttatccacc agtctatcac cggactttac
     9361 gagaccagaa tcgatctttc tcagcttgga ggagataaga gaccagctgc taccaagaag
     9421 gctggacagg ctaagaagaa gaagtgactg cagatcgttc aaacatttgg caataaagtt
     9481 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     9541 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     9601 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     9661 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcaggcc tgtcgacgcc
     9721 cgggcggtac cgcgatcgct cgcgacctgc aggcataaag ccgtcagtgt ccgcataaag
     9781 aaccacccat aatacccata atagctgttt gccatcgcta ccttaggacc gttatagtta
     9841 attaccctgt tatccctatt aattaagagc tcgctacctt aagagaggat atcggcgcgc
     9901 ctctagaatt taaatggatc ctacgtactc gaggaattct gagatttttc aaatcagtgc
     9961 gcaagacgtg acgtaagtat ccgagtcagt ttttattttt ctactaattt ggtcgtttat
    10021 ttcggcgtgt aggacatggc aaccgggcct gaatttcgcg ggtattctgt ttctattcca
    10081 actttttctt gatccgcagc cattaacgac ttttgaatag atacgctgac acgccaagcc
    10141 tcgctagtca aaagtgtacc aaacaacgct ttacagcaag aacggaatgc gcgtgacgct
    10201 cgcggtgacg ccatttcgcc ttttcagaaa tggataaata gccttgcttc ctattatatc
    10261 ttcccaaatt accaatacat tacactagca tctgaatttc ataaccaatc tcgatacacc
    10321 aaatcgaatc gatgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg
    10381 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc
    10441 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct
    10501 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct
    10561 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc
    10621 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac
    10681 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc
    10741 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga
    10801 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg
    10861 tcctgcccgt caccgagatt tgactgcaga atcttggact cccatgttgg caaaggcaac
    10921 caaacaaaca atgaatgatc cgctcctgca tatggggcgg tttgagtatt tcaactgcca
    10981 tttgggctga attgaagaca tgctcctgtc agaaattccg tgatcttact caatattcag
    11041 taatctcggc caatatccta aatgtgcgtg gctttatctg tctttgtatt gtttcatcaa
    11101 ttcatgtaac gtttgctttt cttatgaatt ttcaaataaa ttatcagatc cgagctcgaa
    11161 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
    11221 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
    11281 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
    11341 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
    11401 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
    11461 gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga
    11521 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
    11581 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
    11641 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
    11701 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
    11761 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
    11821 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
    11881 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
    11941 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
    12001 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
    12061 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
    12121 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
    12181 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
    12241 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
    12301 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
    12361 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
    12421 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
    12481 gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    12541 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    12601 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    12661 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    12721 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    12781 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    12841 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    12901 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    12961 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    13021 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    13081 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    13141 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    13201 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    13261 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    13321 gaagcggaag
//

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