| 质粒名称: | pAL119-TK |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21911 |
| 存储实验室: | Maria Castro |
|
载体骨架基本信息 |
|
| 载体名称: | pAL119 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7337 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 腺病毒表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HSV Type 1 thymidine kinase,HSV1-TK,TK |
| 物种来源: | Herpes Simplex Type 1 |
| 插入大小: | 1142 bp |
| 基因突变: | -- |
| GenBank编号 | FJ655139 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI (未破坏) |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAL119-TK was a gift from Maria Castro (Addgene plasmid # 21911 ; http://n2t.net/addgene:21911 ; RRID:Addgene_21911) |
| 参考文献部分: | Chronic brain inflammation and persistent herpes simplex virus 1 thymidine kinase expression in survivors of syngeneic glioma treated by adenovirus-mediated gene therapy: implications for clinical trials. Dewey RA, Morrissey G, Cowsill CM, Stone D, Bolognani F, Dodd NJ, Southgate TD, Klatzmann D, Lassmann H, Castro MG, Loewenstein PR. Nat Med. 1999 Nov . 5(11):1256-63. 10.1038/15207 PubMed 10545991 |
买家导航
pAL119TK载体基本信息
pAL119TK载体图谱质粒图谱和多克隆位点信息
pAL119TK载体简介
pAL119TK载体序列
LOCUS pAL119-TK 8478 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAL119-TK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8478)
AUTHORS Dewey RA, Morrissey G, Cowsill CM, Stone D, Bolognani F, Dodd NJ,
Southgate TD, Klatzmann D, Lassmann H, Castro MG, Loewenstein PR
TITLE Chronic brain inflammation and persistent herpes simplex virus 1
thymidine kinase expression in survivors of syngeneic glioma treated
by adenovirus-mediated gene therapy: implications for clinical
trials.
JOURNAL Nat Med. 1999 Nov . 5(11):1256-63.
PUBMED 10545991
REFERENCE 2 (bases 1 to 8478)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8478
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..103
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
promoter 577..780
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 731..751
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 777..801
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 876..2006
/codon_start=1
/product="herpes simplex virus thymidine kinase"
/label=HSV TK
/note="confers sensitivity to nucleoside analogs such as
acyclovir or ganciclovir"
/translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
CDS 6030..6221
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(6215..6237)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 6323..6463
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 6378..6397
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(6478..6495)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6649..7237)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6729..6748)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7408..8268)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8031..8050
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8269..8373)
/gene="bla"
/label=AmpR promoter
primer_bind 8441..8459
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
181 gtgtgcgccg gtgtanacng gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
241 taaatttggg cgtaaccnag taanatttgg ccattttcgc gggaaaactg aataagagga
301 agtgaaatct gaataattnt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcnagaag cttgcatgcc
481 tgcaggtcaa ttccctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
601 aatgggcgtg gatagcggtt tgactcacgg ggatttncaa gtctccaccc cattgacgtc
661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
781 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
841 agacaccggg accgatccag cctggggatc ccgacatggc ttcgtacccc tgccatcaac
901 acgcgtctgc gttcgaccag gctgcgcgtt ctcgcggcca tagcaaccga cgtacggcgt
961 tgcgccctcg ccggcagcaa gaagccacgg aagtccgcct ggagcagaaa atgcccacgc
1021 tactgcgggt ttatatagac ggtcctcacg ggatggggaa aaccaccacc acgcaactgc
1081 tggtggccct gggttcgcgc gacgatatcg tctacgtacc cgagccgatg acttactggc
1141 aggtgctggg ggcttccgag acaatcgcga acatctacac cacacaacac cgcctcgacc
1201 agggtgagat atcggccggg gacgcggcgg tggtaatgac aagcgcccag ataacaatgg
1261 gcatgcctta tgccgtgacc gacgccgttc tggctcctca tatcgggggg gaggctggga
1321 gctcacatgc cccgcccccg gccctcaccc tcatcttcga ccgccatccc atcgccgccc
1381 tcctgtgcta cccggccgcg cgatacctta tgggcagcat gaccccccag gccgtgctgg
1441 cgttcgtggc cctcatcccg ccgaccttgc ccggcacaaa catcgtgttg ggggcccttc
1501 cggaggacag acacatcgac cgcctggcca aacgccagcg ccccggcgag cggcttgacc
1561 tggctatgct ggccgcgatt cgccgcgttt acgggctgct tgccaatacg gtgcggtatc
1621 tgcagggcgg cgggtcgtgg cgggaggatt ggggacagct ttcggggacg gccgtgccgc
1681 cccagggtgc cgagccccag agcaacgcgg gcccacgacc ccatatcggg gacacgttat
1741 ttaccctgtt tcgggccccc gagttgctgg cccccaacgg cgacctgtac aacgtgtttg
1801 cctgggcctt ggacgtcttg gccaaacgcc tccgtcccat gcacgtcttt atcctggatt
1861 acgaccaatc gcccgccggc tgccgggacg ccctgctgca acttacctcc gggatggtcc
1921 agacccacgt caccaccccc ggctccatac cgacgatctg cgacctggcg cgcacgtttg
1981 cccgggagat gggggaggct aactgaggat cccacgtcac tattgtatac tctatattat
2041 actctatgtt atactctgta atcctactca ataaacgtgt cacgcctgtg aaaccgtact
2101 aagtctcccg tgtcttctta tcaccatcag gtgacatcct cgcccaggct gtcaatcatg
2161 ccggtatcga ttccagtagc accggcccca cgctgacaac ccactcttgc agcgttagca
2221 gcgcccctct taacaagccg acccccacca gcgtcgcggt tactaacact cctctccccg
2281 acctgcagcc caagcttctc gagtctagag tggaaggtgc tgaggtacga tgagacccgc
2341 accaggtgca gaccctgcga gtgtggcggt aaacatatta ggaaccagcc tgtgatgctg
2401 gatgtgaccg aggagctgag gcccgatcac ttggtgctgg cctgcacccg cgctgagttt
2461 ggctctagcg atgaagatac agattgaggt actgaaatgt gtgggcgtgg cttaagggtg
2521 ggaaagaata tataaggtgg gggtcttatg tagttttgta tctgttttgc agcagccgcc
2581 gccgccatga gcaccaactc gtttgatgga agcattgtga gctcatattt gacaacgcgc
2641 atgcccccat gggccggggt gcgtcagaat gtgatgggct ccagcattga tggtcgcccc
2701 gtcctgcccg caaactctac taccttgacc tacgagaccg tgtctggaac gccgttggag
2761 actgcagcct ccgccgccgc ttcagccgct gcagccaccg cccgcgggat tgtgactgac
2821 tttgctttcc tgagcccgct tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac
2881 aagttgacgg ctcttttggc acaattggat tctttgaccc gggaacttaa tgtcgtttct
2941 cagcagctgt tggatctgcg ccagcaggtt tctgccctga aggcttcctc ccctcccaat
3001 gcggtttaaa acataaataa aaaaccagac tctgtttgga tttggatcaa gcaagtgtct
3061 tgctgtcttt atttaggggt tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg
3121 ttgagggtcc tgtgtatttt ttccaggacg tggtaaaggt gactctggat gttcagatac
3181 atgggcataa gcccgtctct ggggtggagg tagcaccact gcagagcttc atgctgcggg
3241 gtggtgttgt agatgatcca gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct
3301 ttcagtagca agctgattgc caggggcagg cccttggtgt aagtgtttac aaagcggtta
3361 agctgggatg ggtgcatacg tggggatatg agatgcatct tggactgtat ttttaggttg
3421 gctatgttcc cagccatatc cctccgggga ttcatgttgt gcagaaccac cagcacagtg
3481 tatccggtgc acttgggaaa tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg
3541 gagacgccct tgtgacctcc aagattttcc atgcattcgt ccataatgat ggcaatgggc
3601 ccacgggcgg cggcctgggc gaagatattt ctgggatcac taacgtcata gttgtgttcc
3661 aggatgagat cgtcataggc catttttaca aagcgcgggc ggagggtgcc agactgcggt
3721 ataatggttc catccggccc aggggcgtag ttaccctcac agatttgcat ttcccacgct
3781 ttgagttcag atggggggat catgtctacc tgcggggcga tgaagaaaac ggtttccggg
3841 gtaggggaga tcagctggga agaaagcagg ttcctgagca gctgcgactt accgcagccg
3901 gtgggcccgt aaatcacacc tattaccggc tgcaactggt agttaagaga gctgcagctg
3961 ccgtcatccc tgagcagggg ggccacttcg ttaagcatgt ccctgactcg catgttttcc
4021 ctgaccaaat ccgccagaag gcgctcgccg cccagcgata gcagttcttg caaggaagca
4081 aagtttttca acggtttgag accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc
4141 agttccaggc ggtcccacag ctcggtcacc tgctctacgg catctcgatc cagcatatct
4201 cctcgtttcg cgggttgggg cggctttcgc tgtacggcag tagtcggtgc tcgtccagac
4261 gggccagggt catgtctttc cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg
4321 tgaaggggtg cgctccgggc tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg
4381 tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag gtagcatttg accatggtgt
4441 catagtccag cccctccgcg gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc
4501 cgcacgaggg gcagtgcaga cttttgaggg cgtagagctt gggcgcgaga aataccgatt
4561 ccggggagta ggcatccgcg ccgcaggccc cgcagacggt ctcgcattcc acgagccagg
4621 tgagctctgg ccgttcgggg tcaaaaacca ggtttccccc atgctttttg atgcgtttct
4681 tacctctggt ttccatgagc cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc
4741 cgtatacaga cttgagaggc ctgtcctcga ccgatgccct tgagagcctt caacccagtc
4801 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
4861 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
4921 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
4981 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
5041 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
5101 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
5161 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
5221 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
5281 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
5341 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
5401 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
5461 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
5521 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
5581 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
5641 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
5701 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
5761 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
5821 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
5881 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
5941 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
6001 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
6061 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
6121 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
6181 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
6241 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
6301 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
6361 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
6421 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
6481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
6541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
6601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
6661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
6721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
6781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
6841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
6901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
6961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
7021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
7081 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
7141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
7201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
7261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
7321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
7381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
7441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
7501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
7561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
7621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
7681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
7741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
7801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
7861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
7921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
7981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
8041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
8101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
8161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
8221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
8281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
8341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
8401 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
8461 ctttcgtctt caagaatt
//
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pAL119TK载体质粒应用举例
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