| 质粒名称: | pcDNA3-TLR8-YFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13024 |
| 存储实验室: | Doug Golenbock |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3-YFP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6100 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Toll-Like Receptor 8,TLR8 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3177 bp |
| 基因突变: | -- |
| GenBank编号 | NM_016610 |
| 标签/融合蛋白: | YFP（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | GTCTTGTAGTTGCCGTCGTC |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3-TLR8-YFP was a gift from Doug Golenbock (Addgene plasmid # 13024 ; http://n2t.net/addgene:13024 ; RRID:Addgene_13024) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3-TLR8-YFP 9279 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcDNA3-TLR8-YFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9279)
TITLE TLR Signaling Plasmids
REFERENCE 2 (bases 1 to 9279)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9279
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4096..4815
/codon_start=1
/product="enhanced YFP"
/label=EYFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4141..4162)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4402..4421)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4749..4770
/label=EGFP-C
/note="EGFP, forward primer"
promoter complement(4831..4849)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4832..4849)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4869..4886)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4875..5099
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5145..5573
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5232..5251)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5442..5463
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5582..5602)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5587..5917
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5768..5903
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5830..5849
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5984..6778
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6038..6057)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6648..6667
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6952..7073
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6989..7008)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7043..7062
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7122..7138)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7122..7138)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7135..7157)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7146..7162
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7170..7200)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7215..7236
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7353..7370)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7524..8112)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7604..7623)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8283..9143)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8906..8925
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9144..9248)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccatgaagga gtcatctttg caaaatagct cctgcagtct gggaaaggag
961 actaaaaagg aaaacatgtt ccttcagtcg tcaatgctga cctgcatttt cctgctaata
1021 tctggttcct gtgagttatg cgccgaagaa aatttttcta gaagctatcc ttgtgatgag
1081 aaaaagcaaa atgactcagt tattgcagag tgcagcaatc gtcgactaca ggaagttccc
1141 caaacggtgg gcaaatatgt gacagaacta gacctgtctg ataatttcat cacacacata
1201 acgaatgaat catttcaagg gctgcaaaat ctcactaaaa taaatctaaa ccacaacccc
1261 aatgtacagc accagaacgg aaatcccggt atacaatcaa atggcttgaa tatcacagac
1321 ggggcattcc tcaacctaaa aaacctaagg gagttactgc ttgaagacaa ccagttaccc
1381 caaataccct ctggtttgcc agagtctttg acagaactta gtctaattca aaacaatata
1441 tacaacataa ctaaagaggg catttcaaga cttataaact tgaaaaatct ctatttggcc
1501 tggaactgct attttaacaa agtttgcgag aaaactaaca tagaagatgg agtatttgaa
1561 acgctgacaa atttggagtt gctatcacta tctttcaatt ctctttcaca cgtgccaccc
1621 aaactgccaa gctccctacg caaacttttt ctgagcaaca cccagatcaa atacattagt
1681 gaagaagatt tcaagggatt gataaattta acattactag atttaagcgg gaactgtccg
1741 aggtgcttca atgccccatt tccatgcgtg ccttgtgatg gtggtgcttc aattaatata
1801 gatcgttttg cttttcaaaa cttgacccaa cttcgatacc taaacctctc tagcacttcc
1861 ctcaggaaga ttaatgctgc ctggtttaaa aatatgcctc atctgaaggt gctggatctt
1921 gaattcaact atttagtggg agaaatagcc tctggggcat ttttaacgat gctgccccgc
1981 ttagaaatac ttgacttgtc ttttaactat ataaagggga gttatccaca gcatattaat
2041 atttccagaa acttctctaa acttttgtct ctacgggcat tgcatttaag aggttatgtg
2101 ttccaggaac tcagagaaga tgatttccag cccctgatgc agcttccaaa cttatcgact
2161 atcaacttgg gtattaattt tattaagcaa atcgatttca aacttttcca aaatttctcc
2221 aatctggaaa ttatttactt gtcagaaaac agaatatcac cgttggtaaa agatacccgg
2281 cagagttatg caaatagttc ctcttttcaa cgtcatatcc ggaaacgacg ctcaacagat
2341 tttgagtttg acccacattc gaacttttat catttcaccc gtcctttaat aaagccacaa
2401 tgtgctgctt atggaaaagc cttagattta agcctcaaca gtattttctt cattgggcca
2461 aaccaatttg aaaatcttcc tgacattgcc tgtttaaatc tgtctgcaaa tagcaatgct
2521 caagtgttaa gtggaactga attttcagcc attcctcatg tcaaatattt ggatttgaca
2581 aacaatagac tagactttga taatgctagt gctcttactg aattgtccga cttggaagtt
2641 ctagatctca gctataattc acactatttc agaatagcag gcgtaacaca tcatctagaa
2701 tttattcaaa atttcacaaa tctaaaagtt ttaaacttga gccacaacaa catttatact
2761 ttaacagata agtataacct ggaaagcaag tccctggtag aattagtttt cagtggcaat
2821 cgccttgaca ttttgtggaa tgatgatgac aacaggtata tctccatttt caaaggtctc
2881 aagaatctga cacgtctgga tttatccctt aataggctga agcacatccc aaatgaagca
2941 ttccttaatt tgccagcgag tctcactgaa ctacatataa atgataatat gttaaagttt
3001 tttaactgga cattactcca gcagtttcct cgtctcgagt tgcttgactt acgtggaaac
3061 aaactactct ttttaactga tagcctatct gactttacat cttcccttcg gacactgctg
3121 ctgagtcata acaggatttc ccacctaccc tctggctttc tttctgaagt cagtagtctg
3181 aagcacctcg atttaagttc caatctgcta aaaacaatca acaaatccgc acttgaaact
3241 aagaccacca ccaaattatc tatgttggaa ctacacggaa acccctttga atgcacctgt
3301 gacattggag atttccgaag atggatggat gaacatctga atgtcaaaat tcccagactg
3361 gtagatgtca tttgtgccag tcctggggat caaagaggga agagtattgt gagtctggag
3421 ctaacaactt gtgtttcaga tgtcactgca gtgatattat ttttcttcac gttctttatc
3481 accaccatgg ttatgttggc tgccctggct caccatttgt tttactggga tgtttggttt
3541 atatataatg tgtgtttagc taaggtaaaa ggctacaggt ctctttccac atcccaaact
3601 ttctatgatg cttacatttc ttatgacacc aaagatgcct ctgttactga ctgggtgata
3661 aatgagctgc gctaccacct tgaagagagc cgagacaaaa acgttctcct ttgtctagag
3721 gagagggatt gggacccggg attggccatc atcgacaacc tcatgcagag catcaaccaa
3781 agcaagaaaa cagtatttgt tttaaccaaa aaatatgcaa aaagctggaa ctttaaaaca
3841 gctttttact tggctttgca gaggctaatg gatgagaaca tggatgtgat tatatttatc
3901 ctgctggagc cagtgttaca gcattctcag tatttgaggc tacggcagcg gatctgtaag
3961 agctccatcc tccagtggcc tgacaacccg aaggcagaag gcttgttttg gcaaactctg
4021 agaaatgtgg tcttgactga aaatgattca cggtataaca atatgtatgt cgattccatt
4081 aagcaatacc tcgagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
4141 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
4201 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
4261 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgaagtgctt cgcccgctac
4321 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
4381 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
4441 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
4501 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
4561 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
4621 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
4681 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
4741 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
4801 gagctgtaca agtaatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
4861 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
4921 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
4981 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
5041 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
5101 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
5161 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
5221 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
5281 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
5341 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
5401 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
5461 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
5521 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
5581 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat
5641 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
5701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
5761 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
5821 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
5881 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
5941 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
6001 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
6061 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
6121 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
6181 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
6241 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
6301 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
6361 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
6421 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
6481 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
6541 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
6601 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
6661 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
6721 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
6781 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
6841 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
6901 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
6961 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
7021 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
7081 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
7141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
7201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
7261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
7321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
7381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
7441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
7501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
7561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
7621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
7681 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
7741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
7801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
7861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
7921 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
7981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
8041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
8101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
8161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
8221 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
8281 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
8341 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
8401 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
8461 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
8521 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
8581 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
8641 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
8701 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
8761 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
8821 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
8881 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
8941 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
9001 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
9061 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
9121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
9181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
9241 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
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